[bionet.molbio.genbank.updates] Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1

GenBank-Updates@genbank.bio.net (01/16/91)

LOCUS       RATTRO06      101 bp ds-DNA             ROD       16-JAN-1991
DEFINITION  Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1
            gene, alternative exon 6.
ACCESSION   L00377 M12080 M14127
KEYWORDS    alternate splicing; tropomyosin.
SEGMENT     6 of 11
SOURCE      Rat adult rat skeletal muscle, cDNA to mRNA, clone pRSkM-22 [Mol.
            Cell. Biol. 6, 3582-3595 (1986)]; embryonic fibroblast, cDNA to
            mRNA [J. Biol. Chem. 260, 14440-14445 (1985)]; DNA, library of
            R.O.Hynes [Mol. Cell. Biol. 6, 3582-3595 (1986)].
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 101)
  AUTHORS   Helfman,D.M., Cheley,S., Kuismanen,E., Finn,L.A. and
            Yamawaki-Kataoka,Y.
  TITLE     Nonmuscle and muscle tropomyosin isoforms are expressed from a
            single gene by alternative RNA splicing and polyadenylation
  JOURNAL   Mol. Cell. Biol. 6, 3582-3595 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 16 to 91)
  AUTHORS   Valiante,N.M. and Caton,A.J.
  TITLE     A new Igk-V gene family in the mouse
  JOURNAL   Immunogenetics 32, 345-350 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     precursor_RNA   <1..>101
                     /note="beta mRNA and intron"
     precursor_RNA   <1..>101
                     /note="trop1 mRNA and intron"
     intron          <1..15
                     /note="trop1 intron E"
     intron          <1..>101
                     /note="beta intron E"
     exon            16..91
                     /note="embryonic fibroblast tropomyosin 1, exon 6"
     intron          92..>101
                     /note="trop1 intron F"
BASE COUNT       22 a     31 c     33 g     15 t
ORIGIN      About 150 bp after segment 5.
        1 gccccctgcc cccagccgag ctaggcagct ggaggaggag cttcgaacca tggaccaggc
       61 tctcaagtcg ctgatagcct cagaggagga ggtagtagcc t
//

GenBank-Updates@genbank.bio.net (01/16/91)

LOCUS       RATTRO05       96 bp ds-DNA             ROD       16-JAN-1991
DEFINITION  Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1
            gene, exon 5.
ACCESSION   L00376 M12080 M14127
KEYWORDS    alternate splicing; tropomyosin.
SEGMENT     5 of 11
SOURCE      Rat adult rat skeletal muscle, cDNA to mRNA, clone pRSkM-22 [Mol.
            Cell. Biol. 6, 3582-3595 (1986)]; embryonic fibroblast, cDNA to
            mRNA [J. Biol. Chem. 260, 14440-14445 (1985)]; DNA, library of
            R.O.Hynes [Mol. Cell. Biol. 6, 3582-3595 (1986)].
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 96)
  AUTHORS   Helfman,D.M., Cheley,S., Kuismanen,E., Finn,L.A. and
            Yamawaki-Kataoka,Y.
  TITLE     Nonmuscle and muscle tropomyosin isoforms are expressed from a
            single gene by alternative RNA splicing and polyadenylation
  JOURNAL   Mol. Cell. Biol. 6, 3582-3595 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 16 to 86)
  AUTHORS   Valiante,N.M. and Caton,A.J.
  TITLE     A new Igk-V gene family in the mouse
  JOURNAL   Immunogenetics 32, 345-350 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     precursor_RNA   <1..>96
                     /note="beta mRNA and intron"
     precursor_RNA   <1..>96
                     /note="trop1 mRNA and intron"
     intron          <1..15
                     /note="beta intron D"
     intron          <1..15
                     /note="trop1 intron D"
     exon            16..86
                     /note="skeletal muscle beta-tropomyosin, exon 5"
     exon            16..86
                     /note="embryonic fibroblast tropomyosin 1, exon 5"
     intron          87..>96
                     /note="beta intron E"
     intron          87..>96
                     /note="trop1 intron E"
BASE COUNT       21 a     22 c     38 g     15 t
ORIGIN      About 100 bp after segment 3.
        1 cccgcttccc ctcaggtggc caggaagctg gtgatcctgg aaggagagct ggagcgctct
       61 gaagagagag ccgaggtggc tgagaggtag ggatac
//

GenBank-Updates@genbank.bio.net (01/16/91)

LOCUS       RATTRO02      151 bp ds-DNA             ROD       16-JAN-1991
DEFINITION  Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1
            gene, exon 2.
ACCESSION   L00373 M12080 M14127
KEYWORDS    alternate splicing; tropomyosin.
SEGMENT     2 of 11
SOURCE      Rat adult rat skeletal muscle, cDNA to mRNA, clone pRSkM-22 [Mol.
            Cell. Biol. 6, 3582-3595 (1986)]; embryonic fibroblast, cDNA to
            mRNA [J. Biol. Chem. 260, 14440-14445 (1985)]; DNA, library of
            R.O.Hynes [Mol. Cell. Biol. 6, 3582-3595 (1986)].
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 151)
  AUTHORS   Helfman,D.M., Cheley,S., Kuismanen,E., Finn,L.A. and
            Yamawaki-Kataoka,Y.
  TITLE     Nonmuscle and muscle tropomyosin isoforms are expressed from a
            single gene by alternative RNA splicing and polyadenylation
  JOURNAL   Mol. Cell. Biol. 6, 3582-3595 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 16 to 141)
  AUTHORS   Valiante,N.M. and Caton,A.J.
  TITLE     A new Igk-V gene family in the mouse
  JOURNAL   Immunogenetics 32, 345-350 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     precursor_RNA   <1..>151
                     /note="beta mRNA and intron"
     precursor_RNA   <1..>151
                     /note="trop1 mRNA and intron"
     intron          <1..15
                     /note="beta intron A"
     intron          <1..15
                     /note="trop1 intron A"
     exon            16..141
                     /note="skeletal muscle beta-tropomyosin, exon 2"
     exon            16..141
                     /note="embryonic fibroblast tropomyosin 1, exon 2"
     intron          142..>151
                     /note="beta intron B"
     intron          142..>151
                     /note="trop1 intron B"
BASE COUNT       45 a     32 c     53 g     21 t
ORIGIN      About 0.5 kb after segment 1.
        1 ccccttcttc cacagctgga ggaagagcag caggccctcc agaagaaact gaaggggaca
       61 gaggatgagg tggaaaagta ttccgagtct gtgaaggatg cccaggagaa actggagcag
      121 gccgagaaga aggccaccga tgtgagtgtg g
//

GenBank-Updates@genbank.bio.net (01/16/91)

LOCUS       RATTRO03      159 bp ds-DNA             ROD       16-JAN-1991
DEFINITION  Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1
            gene, exon 3.
ACCESSION   L00374 M12080 M14127
KEYWORDS    alternate splicing; tropomyosin.
SEGMENT     3 of 11
SOURCE      Rat adult rat skeletal muscle, cDNA to mRNA, clone pRSkM-22 [Mol.
            Cell. Biol. 6, 3582-3595 (1986)]; embryonic fibroblast, cDNA to
            mRNA [J. Biol. Chem. 260, 14440-14445 (1985)]; DNA, library of
            R.O.Hynes [Mol. Cell. Biol. 6, 3582-3595 (1986)].
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 159)
  AUTHORS   Helfman,D.M., Cheley,S., Kuismanen,E., Finn,L.A. and
            Yamawaki-Kataoka,Y.
  TITLE     Nonmuscle and muscle tropomyosin isoforms are expressed from a
            single gene by alternative RNA splicing and polyadenylation
  JOURNAL   Mol. Cell. Biol. 6, 3582-3595 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 16 to 149)
  AUTHORS   Valiante,N.M. and Caton,A.J.
  TITLE     A new Igk-V gene family in the mouse
  JOURNAL   Immunogenetics 32, 345-350 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     precursor_RNA   <1..>159
                     /note="beta mRNA and intron"
     precursor_RNA   <1..>159
                     /note="trop1 mRNA and intron"
     intron          <1..15
                     /note="beta intron B"
     intron          <1..15
                     /note="trop1 intron B"
     exon            16..149
                     /note="skeletal muscle beta-tropomyosin, exon 3"
     exon            16..149
                     /note="embryonic fibroblast tropomyosin 1, exon 3"
     intron          150..>159
                     /note="beta intron C"
     intron          150..>159
                     /note="trop1 intron C"
BASE COUNT       39 a     36 c     58 g     26 t
ORIGIN      About 2.5 kb after segment 2.
        1 ccctctgacc cacaggctga agcagatgtg gcttctctga atcgacgcat tcagctcgta
       61 gaggaggagt tggatcgggc acaggagcgc ctggctacag ccttgcaaaa gctggaggag
      121 gctgagaaag ccgctgatga gagcgagagg tggggaagg
//

GenBank-Updates@genbank.bio.net (01/16/91)

LOCUS       RATTRO04      143 bp ds-DNA             ROD       16-JAN-1991
DEFINITION  Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1
            gene, exon 4.
ACCESSION   L00375 M12080 M14127
KEYWORDS    alternate splicing; tropomyosin.
SEGMENT     4 of 11
SOURCE      Rat adult rat skeletal muscle, cDNA to mRNA, clone pRSkM-22 [Mol.
            Cell. Biol. 6, 3582-3595 (1986)]; embryonic fibroblast, cDNA to
            mRNA [J. Biol. Chem. 260, 14440-14445 (1985)]; DNA, library of
            R.O.Hynes [Mol. Cell. Biol. 6, 3582-3595 (1986)].
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 143)
  AUTHORS   Helfman,D.M., Cheley,S., Kuismanen,E., Finn,L.A. and
            Yamawaki-Kataoka,Y.
  TITLE     Nonmuscle and muscle tropomyosin isoforms are expressed from a
            single gene by alternative RNA splicing and polyadenylation
  JOURNAL   Mol. Cell. Biol. 6, 3582-3595 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 16 to 133)
  AUTHORS   Valiante,N.M. and Caton,A.J.
  TITLE     A new Igk-V gene family in the mouse
  JOURNAL   Immunogenetics 32, 345-350 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     precursor_RNA   <1..>143
                     /note="beta mRNA and intron"
     precursor_RNA   <1..>143
                     /note="trop1 mRNA and intron"
     intron          <1..15
                     /note="beta intron C"
     intron          <1..15
                     /note="trop1 intron C"
     exon            16..133
                     /note="skeletal muscle beta-tropomyosin, exon 4"
     exon            16..133
                     /note="embryonic fibroblast tropomyosin 1, exon 4"
     intron          134..>143
                     /note="beta intron D"
     intron          134..>143
                     /note="trop1 intron D"
BASE COUNT       45 a     30 c     47 g     21 t
ORIGIN      About 250 bp after segment 3.
        1 gtccctgctt ctcagaggaa tgaaggtcat tgagaaccgg gccatgaagg atgaggagaa
       61 gatggagctg caggagatgc agctgaaaga agccaagcac atcgctgagg actcagaccg
      121 caaatacgag gaggtgacag cct
//

GenBank-Updates@genbank.bio.net (01/16/91)

LOCUS       RATTRO11      168 bp ds-DNA             ROD       16-JAN-1991
DEFINITION  Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1
            gene, alternative exon 9.
ACCESSION   L00382 M14127
KEYWORDS    alternate splicing; tropomyosin.
SEGMENT     11 of 11
SOURCE      Rat adult rat skeletal muscle, cDNA to mRNA, clone pRSkM-22 [Mol.
            Cell. Biol. 6, 3582-3595 (1986)]; embryonic fibroblast, cDNA to
            mRNA [J. Biol. Chem. 260, 14440-14445 (1985)]; DNA, library of
            R.O.Hynes [Mol. Cell. Biol. 6, 3582-3595 (1986)].
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 168)
  AUTHORS   Helfman,D.M., Cheley,S., Kuismanen,E., Finn,L.A. and
            Yamawaki-Kataoka,Y.
  TITLE     Nonmuscle and muscle tropomyosin isoforms are expressed from a
            single gene by alternative RNA splicing and polyadenylation
  JOURNAL   Mol. Cell. Biol. 6, 3582-3595 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 16 to 168)
  AUTHORS   Valiante,N.M. and Caton,A.J.
  TITLE     A new Igk-V gene family in the mouse
  JOURNAL   Immunogenetics 32, 345-350 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     precursor_RNA   <1..168
                     /note="trop1 mRNA"
     intron          <1..15
                     /note="trop1 intron H"
     CDS             join(L00372:617..730,
                     L00373:16..141,L00374:16..149,L00375:16..133,
                     L00376:16..86,L00377:16..91,L00379:16..78,
                     L00380:16..85,16..98)
                     /note="embryonic fibroblast tropomyosin 1"
     exon            16..98
                     /note="embryonic fibroblast tropomyosin 1, exon 9"
BASE COUNT       43 a     51 c     40 g     34 t
ORIGIN      About 0.85 kb after segment 10.
        1 tgcttcccct cacagagact ttggctagtg ccaaggaaga gaacgtggag attcaccaga
       61 ccttggacca gacccttctg gaactcaaca acctgtaagg gtggccctgc tacctgccga
      121 ccagactaca tagatgcctc ctcaggccaa taaacggatg tctctggc
//

GenBank-Updates@genbank.bio.net (01/16/91)

LOCUS       RATTRO08       88 bp ds-DNA             ROD       16-JAN-1991
DEFINITION  Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1
            gene, exon 7.
ACCESSION   L00379 M12080 M14127
KEYWORDS    alternate splicing; tropomyosin.
SEGMENT     8 of 11
SOURCE      Rat adult rat skeletal muscle, cDNA to mRNA, clone pRSkM-22 [Mol.
            Cell. Biol. 6, 3582-3595 (1986)]; embryonic fibroblast, cDNA to
            mRNA [J. Biol. Chem. 260, 14440-14445 (1985)]; DNA, library of
            R.O.Hynes [Mol. Cell. Biol. 6, 3582-3595 (1986)].
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 88)
  AUTHORS   Helfman,D.M., Cheley,S., Kuismanen,E., Finn,L.A. and
            Yamawaki-Kataoka,Y.
  TITLE     Nonmuscle and muscle tropomyosin isoforms are expressed from a
            single gene by alternative RNA splicing and polyadenylation
  JOURNAL   Mol. Cell. Biol. 6, 3582-3595 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 16 to 78)
  AUTHORS   Valiante,N.M. and Caton,A.J.
  TITLE     A new Igk-V gene family in the mouse
  JOURNAL   Immunogenetics 32, 345-350 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     precursor_RNA   <1..>88
                     /note="beta mRNA and intron"
     precursor_RNA   <1..>88
                     /note="trop1 mRNA and intron"
     intron          <1..15
                     /note="beta intron F"
     intron          <1..15
                     /note="trop1 intron F"
     exon            16..78
                     /note="skeletal muscle beta-tropomyosin, exon 7"
     exon            16..78
                     /note="embryonic fibroblast tropomyosin 1, exon 7"
     variation       74..74
                     /note="a in cDNA [J. Biol. Chem. 260, 14440-14445
                     (1985)],[Mol. Cell. Biol. 6, 3582-3595 (1986)]; g in DNA
                     [Mol. Cell. Biol. 6, 3582-3595 (1986)] (table 1)"
     intron          79..>88
                     /note="beta intron G"
     intron          79..>88
                     /note="trop1 intron G"
BASE COUNT       33 a     14 c     23 g     18 t
ORIGIN      About 150 bp after segment 7.
        1 gtttgtctct taaagtattc caccaaagag gacaaatacg aagaagagat caaacttctg
       61 gaggagaagc tgaaggaggt gagactac
//

GenBank-Updates@genbank.bio.net (01/16/91)

LOCUS       RATTRO01      755 bp ds-DNA             ROD       16-JAN-1991
DEFINITION  Rat skeletal muscle beta-tropomyosin and fibroblast tropomyosin 1
            gene, exon 1.
ACCESSION   L00372 M12080 M14127
KEYWORDS    alternate splicing; tropomyosin.
SEGMENT     1 of 11
SOURCE      Rat adult rat skeletal muscle, cDNA to mRNA, clone pRSkM-22 [Mol.
            Cell. Biol. 6, 3582-3595 (1986)]; embryonic fibroblast, cDNA to
            mRNA [J. Biol. Chem. 260, 14440-14445 (1985)]; DNA, library of
            R.O.Hynes [Mol. Cell. Biol. 6, 3582-3595 (1986)].
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Helfman,D.M., Cheley,S., Kuismanen,E., Finn,L.A. and
            Yamawaki-Kataoka,Y.
  TITLE     Nonmuscle and muscle tropomyosin isoforms are expressed from a
            single gene by alternative RNA splicing and polyadenylation
  JOURNAL   Mol. Cell. Biol. 6, 3582-3595 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 555 to 729)
  AUTHORS   Valiante,N.M. and Caton,A.J.
  TITLE     A new Igk-V gene family in the mouse
  JOURNAL   Immunogenetics 32, 345-350 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     precursor_RNA   505..>755
                     /note="beta mRNA (alt.)"
     precursor_RNA   505..>755
                     /note="trop1 mRNA (alt.)"
     precursor_RNA   507..>755
                     /note="beta mRNA (alt.)"
     precursor_RNA   507..>755
                     /note="trop1 mRNA (alt.)"
     exon            617..730
                     /note="skeletal muscle beta-tropomyosin, exon 1"
     exon            617..730
                     /note="embryonic fibroblast tropomyosin 1, exon 1"
     intron          731..>755
                     /note="beta intron A"
     intron          731..>755
                     /note="trop1 intron A"
BASE COUNT      142 a    245 c    243 g    125 t
ORIGIN      615 bp upstream of NcoI site.
        1 cccggggtaa actgaggcac tcgaggcgtg caggagatcc gcctcccaag agacatttaa
       61 tctgggggga tttacaggaa acttctaaat taagggtagc ggctgctgct agctgaggga
      121 gggcacgcgg tctctgtgcc caggcagctg ccgtgacgtc acgccctgaa atagccccgg
      181 ggccccagcc gcagctgccg ctgggcccgc ctgtcactca gaggaagcgc tgagcccccg
      241 gcccaagggt cttgtagcct ctgctgacca aggtttttac agcccgcagt gagccagttt
      301 tgcttccgac ggctggctgg ggaagaagga ggcgggggcc gggatccgag ggggcggacc
      361 cgaggggtgg gcgcaaaggg tggccgcgcc caagaccggg cagtgcgtct gcccctacaa
      421 ggtttgggcc ttggtggggg agggtcctgg tcccccggct ccgcccggct tctccccgcc
      481 ttttagcgcc cgcgctgctg ggacatcaca gtccctcttg atcctcctaa cctgtcccgg
      541 gtgcatcagt gctctcggct agcccggtct acctactctt caccgctcgc ccgccggccc
      601 actccccacc gcagccatgg acgccatcaa gaagaagatg cagatgctga aactggacaa
      661 ggagaatgcc atcgaccgcg cagagcaggc cgaagccgac aagaagcaag ctgaagaccg
      721 atgcaagcag gtggggccca ccccttcttc cacag
//