GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS RSPNIFHD 1375 bp ds-DNA BCT 16-JAN-1991
DEFINITION R.rubrum dinitrogenase reductase (nifH) gene, complete cds; and
dinitrogenase alpha subunit (nifD') gene, 5' end.
ACCESSION M33774
KEYWORDS dinitrogenase alpha subunit; dinitrogenase reductase.
SOURCE R.rubrum DNA.
ORGANISM Rhodospirillum rubrum
Prokaryota; Bacteria; Gracilicutes; Anoxyphotobacteria; Purple
nonsulfur bacteria.
REFERENCE 1 (bases 1 to 1375)
AUTHORS Lehman,L.J., Fitzmaurice,W.P. and Roberts,G.P.
TITLE The cloning and functional characterization of the nifH gene of
Rhodospirillum rubrum
JOURNAL Gene 95, 143-147 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Gene (1990) In
press] kindly submitted
by L.J.Lehman, 18-APR-1990.
FEATURES Location/Qualifiers
misc_signal 145..160
/note="upstream activating sequence"
misc_signal 175..190
/note="upstream activating sequence"
misc_signal 280..296
/note="ntr promoter"
misc_binding 355..358
/note="ribosome binding site"
CDS 367..1254
/note="dinitrogenase reductase (nifH)"
misc_binding 1357..1361
/note="ribosome binding site"
CDS 1370..>1375
/note="dinitrogenase alpha subunit (nifD)"
BASE COUNT 287 a 404 c 431 g 253 t
ORIGIN
1 ggggatccca aggagggacc ctccgtcctg accaagaact gtgcgttcgt gttttcgccc
61 attcctttat caccgatcgg gcggcgacgg gaagggcgtt ctgggcagta gcgcaccatc
121 gcgcatagcg tcgcgccgcc ccgctgttgg gtccgcgaca gggttttggg gcgttgtcgg
181 gtttgcgaca cggttgaaaa taaccctaca ttccttgtta gatagattta ttcctttgat
241 taaaaacgat aattttcaaa aaaatcgggc aaatcaggtt tggcacgctg ggtgcattaa
301 cggggtgagc gtggcggtgg agcttccccg ccttaggacc ggatcacgaa gaaaaggatt
361 gattccatga gcgcactgcg tcagatcgcg ttctacggca agggcggtat cggcaagtcg
421 acgacgtccc agaacacgct ggccgctctg gtcgaaatgg gccagcggat cctgattgtc
481 ggctgcgatc ccaaggccga ttccacccgc ctgatcctga acaccaagct gcaggacacc
541 gttcttcatc tggccgccga ggccggctcg gtcgaagacc tcgacgtcgc cgatgtggtg
601 aagatcggct acaagggcat caagtgcacc gaatccggtg gtccggagcc cggcgtcggc
661 tgtgccggcc gtggcgtgat caccgccatc aacttccttg aggaaaacgg cgcctacgac
721 gacctcgatt acgtctctta cgacgtgctg ggcgacgtgg tttgcggtgg cttcgccatg
781 ccgatccgcg agaacaaggc tcaggaaatc tacatcgtca tgtcgggcga gatgatggcg
841 ctgtatgccg ccaacaacat cgccaagggc attttgaaat acgcccacac cggtggggtt
901 cgtctgggcg gtctgatctg caacgagcgt cagactgata aggaagtcga actggccgag
961 gccctggccg gccgtttggg ctgccgcctc atccacttcg tgccgcgcga caacggcgtt
1021 cagcacgccg agctgcgccg ccaaacggtg atccaatacg cccccgacag caagcaggcc
1081 ggtgagtatc gcacgctggc gaccaagatc cacaacaact ccggccaggg cgtggtgccg
1141 accccgatca ccatggaaga cctggaagag atgctgatgg aattcggcat catgaagtcc
1201 gacgaagagg cgctggccga gctcgaagcc aaggaatccg ccgccgccaa ttaaggccgt
1261 cgccgtgcga cgttcccccc ggccggccgg tcaggcgcag gggcgacgga agcgggcgac
1321 ctccaagaag gcctcttcgc gaggcgatag tcgatcagag gagagggata tgagc
//