GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS VIBSCRRE 1524 bp ds-DNA BCT 16-JAN-1991
DEFINITION V.alginolyticus Scr repressor protein gene (scrR), complete cds,
and sucrose transport (scrA) protein, 5' end.
ACCESSION M35009
KEYWORDS Scr repressor protein; sucrose transport protein.
SOURCE V.alginolyticus DNA.
ORGANISM Vibrio alginolyticus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1524)
AUTHORS Blatch,G.L., Scholle,R.R. and Woods,D.R.
TITLE Nucleotide sequence and analysis of the vibrio alginolyticus
sucrose uptake-encoding region
JOURNAL Gene 95, 17-23 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by G.L.Blatch, 10-JUN-1990.
Author address: G.L.Blatch
University of Cape Town
Dept. Microbiology
Private Bag
Rondebosch
7700 SOUTH AFRICA
Email: bltgre01.uctvax@f4.n494.z5.fidonet.org
FEATURES Location/Qualifiers
CDS complement(<1..36)
/note="sucrose transport protein (scrA)"
misc_feature 12..71
/note="ScrR helix-turn-helix motif"
misc_feature 78..91
/note="repressor operator site (put.)"
misc_signal 162..167
/note="scrR -10 region"
misc_signal 178..183
/note="scrA -10 region"
misc_signal 190..195
/note="scrR -10 region"
misc_signal 203..208
/note="scrA -35 region"
misc_feature 229..242
/note="repressor operator site (put.)"
misc_binding 264..267
/note="scrR ribosomal binding site"
CDS 271..555
/note="Scr repressor protein (scrR)"
misc_signal 742..747
/note="ORF5 -35 region"
misc_signal 762..767
/note="ORF5 -35 region"
misc_binding 780..785
/note="ORF5 ribosomal binding site"
CDS 791..1090
/note="ORF5"
BASE COUNT 466 a 344 c 321 g 393 t
ORIGIN
1 agttaacaac tcttttgcta ctgccggata attcatttca taccccattg gattattatc
61 tgagcttgca gaattttcgg gaacgtttgc aaaacagatt tttgtcaata aaaccaaatg
121 atcaaagata aagcagagag aatgtgagaa tgatcgtcaa attctctccc taagagcagt
181 ataggaggtt agaatcgcta cttatcaaag agcctttcat cttctctttg caaacaatcc
241 caaaaacgaa aaacagaatg gataggaaca atggcgagtc tgcatgatgt cgcccgtctg
301 gctggcgtat caaaatcaac agtgtcacgg gttataaacg atgagtatgg cgtaaaagaa
361 gcgacaaaac agaaggtccg ccaagcagta gcggagtgtg gctatgtgcc gaaccaagtc
421 gctaaagatc ttaaagaaga aagctctgcc attattgttc aagacaaaac aggcaaagtg
481 ctgtttgtaa aagaaggcgc acttgagcaa gatgaaattg caaaggtgat cgagctaatt
541 aaacagaaca tctagtctcg ttgcttggct ccttaatatc cgtaatacac aatataagga
601 gccatgcgac caaacttaaa gctgagattt ctgttgcccc tagttgaacg cttagtgatt
661 ccaccaagga gactgtctca gattcggcga catcacatgt gttgacgtaa attttcccac
721 tagccaacaa aagagtgaac ttttactcat tatgggtgtt atattataac agtcggtaag
781 aggagctcag atgtaccacc accagcaaaa gatacggaag cattggcatc gcactgtttt
841 atttttcagt gtcgcgttgc tgatcgcttg gaactttgcg gtaatccttc atcaagttga
901 tctgactccc gaacaccaca cacaccatca ttgccagcta ttttctgggg ttcagcacgg
961 catagccaaa gctcaaccga ccctatcgac gccaacattt acgcgcatcc aataccatga
1021 tgtctttcag cgccttgtta atagtgaaga cattcgtggt gcagctcgtg ccccgcctta
1081 ttttgcttaa tttccaaact attccacact caacataatt tgtgcgtatt cctgcacctc
1141 acgtgtcagc gaatcgcacc ttagtaaata caaatctgat ccggtattgc tcgggttagt
1201 gccagcaaaa ttaggaaaca caaaatgtca tcgaaacata ttttagcgct tgtcattggt
1261 ctctctttat cgacagcagc aaatgcggac gaataccgcc agcacggtgc tcatgtgcat
1321 ggtcacgtcg aatttaatat cgcccaagat ggaaaggatc tgttagtgga gattactgct
1381 cccggtgccg atgtggtcgg tttcgagcat gctccagaaa atgaacaaca agagcaagca
1441 cttaaacaag ccattgccac cctagaagac agcaacacac tgtttgcgat taacgcgcaa
1501 gccaattgtg atattgaaga agct
//