GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS AVIH2AA 3800 bp ds-DNA BCT 16-JAN-1991
DEFINITION A.vinelandii H2 uptake hydrogenase (hoxK), complete cds, and H2
uptake hydrogenase (hoxG), complete cds.
ACCESSION M33152
KEYWORDS H2 uptake hydrogenase.
SOURCE A.vinelandii (strain OP) DNA, clone pALM21.
ORGANISM Azotobacter vinelandii
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Aerobic rods and
cocci; Azotobacteraceae.
REFERENCE 1 (bases 1 to 3800)
AUTHORS Menon,A.L., Stults,L.W., Robson,R.L. and Mortenson,L.E.
TITLE Cloning,sequencing and characterization of the [NiFe]
hydrogenase-encoding structural genes (hoxK and hoxG) from
Azotobacter vinelandii
JOURNAL Gene 96, 67-74 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)
U of Georgia, Dep Biochemistry, Athens, GA 30602] kindly submitted
by R.L.Robson, 22-MAR-1990.
FEATURES Location/Qualifiers
CDS 149..1225
/note="H2 uptake hydrogenase (hoxK) precursor"
sig_peptide 149..283
/note="H2 uptake hydrogenase signal peptide (put.)"
mat_peptide 284..1222
/note="H2 uptake hydrogenase"
CDS 1222..3030
/note="H2 uptake hydrogenase (hoxG)"
BASE COUNT 686 a 1318 c 1213 g 583 t
ORIGIN
1 tgtatcaagc catgacaaaa acatggcatt ggcgcattat tcgtgcggtt ttcattcagc
61 aaccgtgggc catacaaccg gcgcgccgtc atagccgaag gacggtgcgc aggggcgccg
121 ataacgacct ggccacaagg gtaacggcat gtctcgactc gaaactttct atgacgtgat
181 gcggcgtcag ggcatcacgc gccgcagctt tctcaaatat tgcagcctga ccgccgcggc
241 cctgggcctc ggcccggcct tcgccccgcg gatcgcccac gcgatggaaa ccaagccgcg
301 cactccggtg ctctggctgc acggcctgga gtgcacctgc tgctccgagt cgttcatccg
361 ttcggcccac ccgctggtca aggacgtggt gctgtcgatg atctcgctgg actacgacga
421 caccctgatg gccgccgccg gccaccaggc cgaggccgcc ctcgaagaga ccatgcgcaa
481 gtacaagggc gagtacatcc tcgccgtgga gggcaacccg ccgctcaacg aggacggcat
541 gttctgcatc gtcggcggca agccgttcat cgagcagctc aggcatgtgg cgaaggacgc
601 caaggcggtg atcgcctggg gcagttgcgc cagttggggc tgcgtgcagg cggcccggcc
661 caacccgacc caggcggtgc cgatccacaa ggtcatcacc gacaagccga tcgtcaaggt
721 gcccggctgc ccgccgatcg ccgaggtgat gaccggggtg atcacctaca tgctgacctt
781 cggcaagctg cccgagctgg accgccaggg gcggccgaag atgttctacg gccagcgcat
841 ccacgacaag tgctaccgcc gcccgcactt cgacgccggc cagttcgtcg agcactggga
901 cgacgagggc gcgcgcaagg gctactgcct gtacaaggtc ggctgcaagg gcccgaccag
961 ctacaacgcc tgctcgacgg tgcgctggaa cgagggcact tccttcccga tccaggccgg
1021 ccacggctgc atcggctgct cggaggacgg tttctgggac aagggctcgt tctatgaacg
1081 cctgaccacc attccgcagt tcggcatcga gaagaacgcc gacgaaatcg gcgccgccgt
1141 cgccggcggg gtcggcgcgg ccatcgccgc gcatgccgcg gtcaccgcca tcaagcgcct
1201 gcagaacaag ggggatcgcc catgagcagc ctgccgaacg ccagccaact ggacaagtcc
1261 ggcaggcgca tcgtcgtcga cccggtgacc cgcatcgagg gccacatgcg ctgcgaggtc
1321 aacgtcgacg ccagcaacgt gatcaccaac gccgtctcca ccggcaccat gtggcgcggc
1381 ctggaggtca tcctcaaggg ccgcgacccg cgcgacgcct gggccttcgt cgagcgcatc
1441 tgcggcgtct gcaccggcac ccatgcgctg acctcggtgc gcgcggtgga ggatgccctg
1501 gacatccgca tcccctacaa cgcccacctg atccgcaacc tgatggacaa gacgctgcag
1561 gtgcacgacc acatcgtgca cttctaccac ctgcacgcgc tggactgggt caacccggtc
1621 aacgccctga aggccgatcc caaggctacc tccgccctgc agcaggcggt ttcgccggcc
1681 catgccaagt ccagccccgg ctacttccgc gacgtgcaga cgcgcctgaa gaagttcgtc
1741 gagagcggcc agctcggcct gttctccaac ggctactggg acaatccggc ctacaagctg
1801 ccgcccgagg cggacctgat ggccgtggcc cactacctgg aggcgctgga cctgcagaag
1861 gacatcgtca agatccatac catcttcggc ggcaagaacc cgcatccgaa ctacatggtc
1921 ggcggcgtgg cctgcgccat caacctggac gacgtcggcg ccgccggcgc gccggtcaac
1981 atgaccagcc tgaacttcgt cctcgaacgc atccacgagg cccgcgagtt caccaggaac
2041 gtctacctgc cggacgtgct ggcggtcgcc gggatctaca aggactggct gtacggcggc
2101 ggtctggccg cgcacaacct gctgtcctac ggcaccttca ccaaggtgcc ctacgacaag
2161 tccagcgacc tgttgccggc cggcgccatc gtcggcggca attgggacga ggtgctgccg
2221 gtcgacgtgc gcgatcccga ggagatccag gagttcgtca gccactcctg gtacagctac
2281 gccgacgaaa ccaaggggct gcatccctgg gacggcgtca ccgagccgaa attcgagctc
2341 ggcccgaaca ccaagggcag ccgcacccac atccaggaaa tcgacgaggc gcacaagtac
2401 agctggatca aggcgccgcg ctggcgcggc cacgctatgg aggtcggccc gctggcacgt
2461 tacatcatcg cctacgcttc gggccgcgaa tacgtgaagg aacaggtcga ccgctcgctg
2521 gccgccttca accagagcac cggcctgaac ctcggcctca agcagttcct gccctcgacc
2581 ctcggccgca ccctggcgcg cgccctggag tgcgagctgg cggtggacag catgctcgac
2641 gactggcagg ccctggtcgg caacatcaag gccggcgacc gcgccaccgc caacgtcgag
2701 aagtgggacc cgagcacctg gccgaaggag gccaagggcg tgggcatcaa cgaggcgccg
2761 cgcggcgccc tgggccactg gatcaggatc aaggacggca agatcgagaa ctaccaggcg
2821 atcgtgccga ccacctggaa cggcaccccg cgcgaccatc tgggcaacat cggcgcctac
2881 gaggccgcgc tgctcaacac caggatggag cgcccggacg agccggtgga gatcctgcgc
2941 accctgcaca gcttcgaccc ctgcctggcc tgttcgaccc acgtgatgtc gccggacggc
3001 caggagctga cccgggtgaa ggtccgctga accggaggat tgcgcgatgg cactggaaaa
3061 atccctggaa accggcgacg gccaggagaa ggtccgcaag cagaccgcgg tgtacgtcta
3121 cgaggcgccg ctgcgcctct ggcactgggt cacggcgctg tccatcgtcg tgctcggcgt
3181 gaccggctac ttcatcggcg cgccgctgcc gacgatgccc ggcgaggcga tggacaacta
3241 cctgatgggc tacatccgct tcgcccactt cgccgccggc tacgtgctgg cgatcggctt
3301 cctcggccgg gtctactggg ccttcgtcgg caaccaccac gcccgcgagc tgttcctcgt
3361 gccggtgcac cgcaaggcct ggtggaagga gctgtggcac gaggtgcgct ggtacctgtt
3421 cctggaaaag accccgaaga agtacatcgg ccacaacccc ctgggccagt tggcgatgtt
3481 ctgcttcttc gtggtcggcg cggtgttcat gagcgtcacc ggcttcgccc tctacgccga
3541 ggggctgggg cgggacagct gggccgaccg gctgttcggc tgggtgatcc cgctgttcgg
3601 ccagagccag gacgtgcaca cctggcacca cctgggcatg tggtacctcg tcgtcttcgt
3661 catggtgcat gtctacctgg ccgtgcgcga agacatcgtt tcccggcagt cgctgatctc
3721 caccatggtc ggcggctggc ggatgttcaa ggacgaccgg ccggattgag ccccgtgtcg
3781 tcccttccgt ccgggccggt
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