GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS LACUSP45 1592 bp ds-DNA BCT 16-JAN-1991
DEFINITION L.lactis secreted protein (usp45) gene, complete cds.
ACCESSION M60178 M35374
KEYWORDS secreted protein.
SOURCE L.lactis lactis (strain MG1363) DNA.
ORGANISM Lactococcus lactis
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 1592)
AUTHORS Van Asseldonk,M., Rutten,G., Oteman,M., Siezen,R.J., De Vos,W.M.
and Simons,G.
TITLE Cloning of usp45, a gene encoding a secreted protein from
Lactococcus lactis subsp. lactis MG1363
JOURNAL Gene 95, 155-160 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 25..30
/gene="usp45"
-10_signal 45..50
/gene="usp45"
mRNA 57..1513
/gene="usp45"
/partial
/product="secreted protein"
CDS 101..1486
/gene="usp45"
/codon_start=101
/product="secreted protein"
sig_peptide 101..181
/gene="usp45"
/codon_start=101
mat_peptide 182..1483
/gene="usp45"
/codon_start=182
/product="secreted protein"
terminator 1514..1553
/gene="usp45"
BASE COUNT 579 a 291 c 308 g 414 t
ORIGIN
1 gtgttttgta atcataaaga aatattaagg tggggtagga atagtataat atgtttattc
61 aaccgaactt aatgggagga aaaattaaaa aagaacagtt atgaaaaaaa agattatctc
121 agctatttta atgtctacag tgatactttc tgctgcagcc ccgttgtcag gtgtttacgc
181 tgacacaaac tcagatattg ctaaacaaga tgcgacaatt tcaagcgcgc aatctgctaa
241 agcacaagca caagcacaag ttgatagctt gcaatcaaaa gttgacagct tacaacaaaa
301 gcaaacaagt actaaagcac aaatcgctaa aatcgaaagc gaactgaaag cacttaatgc
361 tcaaattgct actttgaacg aaagtatcaa agaacgtaca aagacattgg aagctcaagc
421 acgtagtgct caagttaaca gctcagcaac aaattatatg gatgctgttg ttaattcaaa
481 atctttgaca gatgttattc aaaaagtaac agctattgct actgtttcta gtgccaacaa
541 acaaatcttg gaacaacaag aaaaagagca aaaagagctt agccaaaagt cagaaactgt
601 taaaaagaac tacaaccagt tcgtttctct ttcacaaagt ttggattctc aagctcaaga
661 attgacttca caacaagctg aactcaaagt tgcgactttg aactatcaag caacaattgc
721 aactgcgcaa gataaaaaac aagctttatt agatgaaaaa gcagctgcag aaaaagcagc
781 tcaagaagca gctaaaaaac aagcggctta tgaagctcaa caaaaagaag cagcacaagc
841 acaagcagct tcaacagcag caactgctaa agctgtagaa gcagcaactt catcagcttc
901 tgcttcatct agtcaagctc cacaagtaag tacaagcact gataatacaa catcaaatgc
961 tagtgcctca aacagttcta atagttcatc aaactcaagt tcaagttcta gcagttcatc
1021 aagctcaagc tcaagctcaa gtaattctaa tgctggtggg aatacaaatt caggcactag
1081 tactggaaat actggaggaa caactactgg tggtagcggt ataaatagtt caccaattgg
1141 aaatccttat gctggtggtg gatgtactga ctatgtatgg caatactttg ctgcacaagg
1201 aatttatatc agaaatatca tgcctggtaa tggtggacaa tgggcttcta atggacctgc
1261 ccaaggcgtg ctccatgttg taggagctgc tcctggtgtt atcgcatcaa gcttctcagc
1321 tgattttgtt ggatatgcaa actcacctta cggtcacgta gctattgtaa aatcagttaa
1381 ttcagatggt acaattacta tcaaagaagg cggatatggt acaacttggt ggggacatga
1441 acgtactgta agtgcgtctg gtgttacttt cttgatgcca aactagaaaa aagtcttaat
1501 aaataaaaaa tagtggtttg atagtgggga ataattttcc ttctgtcaaa tcatttttta
1561 ttattgtggt ataataataa ggaaaaatga ta
//