GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS SCNGAM1 3751 bp ds-DNA PLN 16-JAN-1991
DEFINITION S.occidentalis glucoamylase (GAM1) gene, complete cds.
ACCESSION M60207 M34666
KEYWORDS glucoamylase.
SOURCE S.occidentalis (ATCC 26076) DNA.
ORGANISM Schwanniomyces occidentalis
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 3751)
AUTHORS Dohmen,J.R., Strasser,A.W.M., Dahlems,U.M. and Hollenberg,C.
TITLE Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1)
and its expression in saccharomyces cerevisiae
JOURNAL Gene 95, 111-121 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
TATA_signal 257..262
/gene="GAM1"
mRNA 299..3199
/note="minor mRNA start"
/gene="GAM1"
/partial
/product="glucoamylase"
mRNA 304..3199
/note="minor mRNA start"
/gene="GAM1"
/partial
/product="glucoamylase"
mRNA 312..3199
/note="major mRNA start"
/gene="GAM1"
/product="glucoamylase"
mRNA 315..3199
/note="minor mRNA start"
/gene="GAM1"
/product="glucoamylase"
CDS 323..3199
/gene="GAM1"
/codon_start=323
/product="glucoamylase"
sig_peptide 323..388
/gene="GAM1"
/codon_start=323
CDS 389..3196
/gene="GAM1"
/codon_start=389
/product="glucoamylase"
BASE COUNT 1132 a 662 c 657 g 1300 t
ORIGIN
1 agatctacat tttaaacccc agtctactcc agatattgga gtataacccc attcttaccg
61 ttatatccat gacccgcatc gaaattttca aaggatttcg aggaaattct ttcctaaaat
121 acgaagtgtt attggtgatt caattactac ggaaactact catatggtag tagagttggt
181 gaatgtagcg caattgtaat ttgcgaagtt atagtaatag tttggcaaac tggagaattt
241 ttcattattg ggaaaatata aataaaggca agtatccatt gaaattttaa aatgaactca
301 tgactgtatt ataacaagca agatgatttt tctgaagctg attaaaagta tagtaattgg
361 tttgggatta gttagtgcta tccaagcagc ccctgcctct tcgattggat ctagtgcttc
421 agcatctagt tcaagtgaga gttctcaggc tacaattccc aatgatgtaa cattaggtgt
481 taaacaaatt cctaatatct ttaatgactc tgctgtcgat gctaatgcag ctgctaaagg
541 gtatgacttg gtaaatgtta ctaatactcc aagaggatta accggtatct taaaattaaa
601 agaagctacc aatatttatg gttatgattt tgattattta aacttaactg ttgaatacca
661 agctgatacc agattaaacg ttcatattga accaactgat ttatctgatg tatttgtttt
721 accagagcat ttagttgtta aaccactggt ggaaggtgat gcacaatctt ataacttcga
781 caattccgat ttggttttcg aatactctaa tactgacttc tcctttgaag ttattagatc
841 atctactaaa gaagttttat tttctactaa aggtaatcca ttggtttttt caaatcaatt
901 cattcaattc aattcgtcat tgccaaagaa ccatgttatt actggtcttg gtgaatctat
961 tcacggttta gttaacgaac caggtagcgt taaaacatta tttgctaatg atgttggtga
1021 tccaatcgat ggtaatattt atggtgtcca tccagtttat cttgatcaaa gatatgacac
1081 tgaaactacc catgctgttt attggagaac ttctgctatt caagaagtat taatcggtga
1141 ggaatctatt acttggagag ctctttcagg tgttattgat ttatacttct ttagtggtcc
1201 tacaccaaaa gatgccattc aacagtatgt caaagagatt ggtttaccag ctttccaacc
1261 atactggtcg ttaggttacc atcaatgtag atggggttac gatactatcg aaaaattatc
1321 tgaagttgtt gaaaacttca agaaatttaa tattccatta gaaactatct ggtcagacat
1381 tgattacatg gactcttata aagatttcac ttatgatcca cacagattcc cactagatga
1441 atatcgtaaa ttccttgatg agttgcacaa aaataatcaa cactatgttc ctattttgga
1501 tgctgctatt tacgttccaa acccaaacaa tgctacggat aacgaatacc aacctttcca
1561 ctatggtaat gaaaccgatg tcttcttaaa gaatccagat ggttcattat atattggtgc
1621 tgtttggcag gttacactgt tttccagatt tcttagcaga aaacattcag atatggataa
1681 agtcattaaa gattggtatg aattaactcc ttttgatggt atttgggctg atatgaatga
1741 agtctcatca ttctgtgttg gttcttgtgg tactggtaaa tacttcgaaa acccagcata
1801 tcctccattt actgttggaa gtaaagctac ctcttatcca gttggtttcg atgtttctaa
1861 cgcatctgaa tggaaatcta ttcaaagctc aatttctgct actgctaaga cttcttcaac
1921 ttcttccgta tcgtcgtctt catccacaat cgattatatg aacactttag ctccaggtaa
1981 aggtaatatt aattatccac catatgctat ttacaacatg caaggtgact ccgatcttgc
2041 tactcatgca gtatctccaa atgctacaca tgctgatggt acagttgaat atgatattca
2101 caatctttat ggttacttgc aagaaaatgc tacttatcat gcattattgg aagtttttcc
2161 taacaagaga ccattcatga tttccagatc aacctttcca cgcgctggta aatggaccgg
2221 ccattggggt ggtgacaaca ctgctgattg ggcttatgct tacttctcta tccctcaagc
2281 attctcaatg ggtattgctg gccttccatt ctttggtgcc gatgtttgtg gtttcaatgg
2341 taattctgat tctgaattat gttcaagatg gatgcaatta ggttctttct tcccattcta
2401 cagaaaccac aactatttag gtgctattga tcaggaacca tatgtctggg aatcagttgc
2461 tgaagctact agaacttcta tggccattag atacttatta ttaccatatt actacacttt
2521 attacatgaa tctcatacta ctggtttacc aatcttaaga gctttctcgt ggcaattccc
2581 taacgatcgt tccttaagtg gtgtcgataa ccaatttttt gtcggtgatg gtttagttgt
2641 tactcctgtc ttagaacctg gtgttgataa ggttaaaggt gttttcccag gagctggtaa
2701 agaggaagtt tactacgact ggtacaccca aagagaagtt cactttaaag acggtaagaa
2761 tgaaacttta gatgcaccat taggtcatat tccattacac attagaggtg gtaacgtctt
2821 gccaactcaa gagccaggtt atactgttgc tgagtcaaga caaaatccat ttggtttaat
2881 tgtcgcttta gataacgatg gcaaagctca aggtagctta taccttgatg atggtgaatc
2941 attagtagta gactcttcat tgttggttag tttctctgtt tctgataaca cattatcagc
3001 atctccatct ggtgactata aagctgatca acctttagct aatgttacca tcttaggggt
3061 tggccataaa ccaaaatcag ttaaatttga aaacgctaat gttgatttca cctacaagaa
3121 atcaaccgtt ttcgttactg gcttagataa atacaccaag gatggtgcat tttctaagga
3181 tttcaccatt acttggtaat tttaacatcc acttagttca attccattct tttctttttc
3241 ccgtgaaatt ctgaatttga aattatttga atgatatcat tttagttttc ttcagcttat
3301 gctatgttta tttcgatttt aaatgttaaa agttttttat gtttatgttg ttttattgat
3361 gtagttgata aaatatagca aatacatcga aaaatttgcg atgaaatttt gcagctcatt
3421 agaaatgtag tcaatcatta gtcacattgg accactatat aacaaacaac aactattcca
3481 agaaaaatat atgtaaggat actagatcat aaattcttat tgactttgtt ttttttaaca
3541 atagttacat aaggaatatt cgtttactac aaaaccattg gtcttgtaaa gaagcagacg
3601 aggcgtatgt ttgtggttgc ggccgctaat actagtttac aaagctcacc ttatcattgt
3661 gctagacttt cttttcaacg aaaggaaagc aaaagtagat gactgctcta ctttaattca
3721 attaagataa atttggtatt ttgctagatc t
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