GenBank-Updates@genbank.bio.net (01/17/91)
LOCUS PFL261CAT 4589 bp ds-DNA circular SYN 17-JAN-1991 DEFINITION Plasmid pFL261 cloning vector cat gene, encoding chloramphenicol acetyltransferase, complete cds. ACCESSION M29363 KEYWORDS cloning vector. SOURCE Plasmid p15A-derived plasmid pFL261 DNA. ORGANISM Cloning vector Artificial sequences; Cloning vehicles. REFERENCE 1 (bases 1 to 4589) AUTHORS Larimer,F.W., Mural,R.J. and Soper,T.S. TITLE ; JOURNAL Unpublished (1989) ORNL, PO Box 2009, Oak Ridge, TN 37831 STANDARD full staff_review REFERENCE 2 (sites) AUTHORS Larimer,F.W., Mural,R.J. and Soper,T.S. TITLE Versatile protein engineering vectors for mutagenesis, expression, and hybrid enzyme formation JOURNAL Protein Eng. 3, 227-231 (1989) STANDARD full staff_review COMMENT Draft entry and computer-readable sequence for [Protein Eng. (1989) In press],[Unpublished (1989) ORNL, PO Box 2009, Oak Ridge, TN 37831] kindly submitted by F.W.Larimer, 23-OCT-1989. FEATURES Location/Qualifiers misc_signal 318..345 /note="tac promoter" misc_binding 380..384 /note="ribosomal binding site" rep_origin 3061..3061 /note="p15A origin" BASE COUNT 1133 a 1185 c 1202 g 1069 t ORIGIN 1 gaattctggc gaatcctctg accagccaga aaacgacctt tctgtggtga aaccggatgc 61 tgcaattcag agcgccagca agtgggggac agcagaagac ctgaccgccg cagagtggat 121 gtttgacatg gtgaagacta tcgcaccatc agccagaaaa ccgaattttg ctgggtgggc 181 taacgatatc cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct 241 gttccgctgg gcatgccagg acaacttctg gtccggtaac gtgctgagcc cggccaagct 301 tactccccat ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag 361 cggataacaa tttcacacag gaggaaggat ccatgggtcg accggccgac gcgctgggct 421 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg 481 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg 541 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg 601 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat 661 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga 721 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc 781 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca 841 gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt 901 tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg 961 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga 1021 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt 1081 ttcgtaaagt ctggaaacgc ggaagtcccc tacgtgctgc tgaagttgcc cgcaacagag 1141 agtggaacca accggtgata ccacgatact atgactgaga gtcaacgcca tgagcggcct 1201 catttcttat tctgagttac aacagtccgc accgctgtcc ggtagctcct tccggtgggc 1261 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg 1321 acaggtgccg gcagcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc 1381 gaaacaagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc 1441 ctgtggaaca cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag 1501 gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg 1561 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac 1621 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat 1681 ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg 1741 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca 1801 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg 1861 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccattgt 1921 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact 1981 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc 2041 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc 2101 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta 2161 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc 2221 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt 2281 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt 2341 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc 2401 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct 2461 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc 2521 tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc 2581 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc 2641 ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt 2701 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg 2761 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta 2821 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct 2881 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac 2941 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat 3001 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt 3061 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt 3121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cctggcggct ccctcgtgcg 3181 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc 3241 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc 3301 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 3361 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag 3421 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc 3481 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa 3541 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga 3601 tctcaagaag atcatcttat taatcagata aaatatttct agagctaaga aaccattatt 3661 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc 3721 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 3781 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 3841 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 3901 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta 3961 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 4021 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 4081 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 4141 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 4201 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 4261 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 4321 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 4381 atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctgcgcaac tgttgggaag 4441 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 4501 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 4561 gtgaattgta atacgactca ctatagggc //