GenBank-Updates@genbank.bio.net (01/17/91)
LOCUS PFL261CAT 4589 bp ds-DNA circular SYN 17-JAN-1991
DEFINITION Plasmid pFL261 cloning vector cat gene, encoding chloramphenicol
acetyltransferase, complete cds.
ACCESSION M29363
KEYWORDS cloning vector.
SOURCE Plasmid p15A-derived plasmid pFL261 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 4589)
AUTHORS Larimer,F.W., Mural,R.J. and Soper,T.S.
TITLE ;
JOURNAL Unpublished (1989) ORNL, PO Box 2009, Oak Ridge, TN 37831
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Larimer,F.W., Mural,R.J. and Soper,T.S.
TITLE Versatile protein engineering vectors for mutagenesis, expression,
and hybrid enzyme formation
JOURNAL Protein Eng. 3, 227-231 (1989)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Protein Eng. (1989)
In press],[Unpublished (1989) ORNL, PO Box 2009, Oak Ridge, TN
37831] kindly
submitted by F.W.Larimer, 23-OCT-1989.
FEATURES Location/Qualifiers
misc_signal 318..345
/note="tac promoter"
misc_binding 380..384
/note="ribosomal binding site"
rep_origin 3061..3061
/note="p15A origin"
BASE COUNT 1133 a 1185 c 1202 g 1069 t
ORIGIN
1 gaattctggc gaatcctctg accagccaga aaacgacctt tctgtggtga aaccggatgc
61 tgcaattcag agcgccagca agtgggggac agcagaagac ctgaccgccg cagagtggat
121 gtttgacatg gtgaagacta tcgcaccatc agccagaaaa ccgaattttg ctgggtgggc
181 taacgatatc cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct
241 gttccgctgg gcatgccagg acaacttctg gtccggtaac gtgctgagcc cggccaagct
301 tactccccat ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
361 cggataacaa tttcacacag gaggaaggat ccatgggtcg accggccgac gcgctgggct
421 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
481 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
541 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg
601 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
661 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
721 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
781 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
841 gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt
901 tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
961 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
1021 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
1081 ttcgtaaagt ctggaaacgc ggaagtcccc tacgtgctgc tgaagttgcc cgcaacagag
1141 agtggaacca accggtgata ccacgatact atgactgaga gtcaacgcca tgagcggcct
1201 catttcttat tctgagttac aacagtccgc accgctgtcc ggtagctcct tccggtgggc
1261 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
1321 acaggtgccg gcagcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
1381 gaaacaagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
1441 ctgtggaaca cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag
1501 gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg
1561 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
1621 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat
1681 ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg
1741 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
1801 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg
1861 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccattgt
1921 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
1981 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
2041 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
2101 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
2161 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
2221 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
2281 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
2341 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
2401 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
2461 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
2521 tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc
2581 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
2641 ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
2701 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
2761 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
2821 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
2881 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
2941 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
3001 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
3061 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
3121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cctggcggct ccctcgtgcg
3181 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
3241 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
3301 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
3361 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
3421 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
3481 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
3541 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
3601 tctcaagaag atcatcttat taatcagata aaatatttct agagctaaga aaccattatt
3661 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
3721 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
3781 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
3841 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
3901 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta
3961 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
4021 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
4081 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
4141 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
4201 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
4261 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
4321 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
4381 atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctgcgcaac tgttgggaag
4441 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
4501 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
4561 gtgaattgta atacgactca ctatagggc
//