GenBank-Updates@genbank.bio.net (01/17/91)
LOCUS PLF260BLA 3642 bp ds-DNA circular SYN 17-JAN-1991
DEFINITION Plasmid pFL260 cloning vector bla gene, encoding beta-lactamase,
complete cds.
ACCESSION M29362
KEYWORDS cloning vector.
SOURCE Plasmid ColE1-derived plasmid pFL260 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (sites)
AUTHORS Larimer,F.W., Mural,R.J. and Soper,T.S.
TITLE Versatile protein engineering vectors for mutagenesis, expression,
and hybrid enzyme formation
JOURNAL Protein Eng. 3, 227-231 (1989)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 3642)
AUTHORS Larimer,F.W., Mural,R.J. and Soper,T.S.
TITLE ;
JOURNAL Protein Eng. 3, 227-231 (1989)
STANDARD full staff_review
COMMENT
[Protein Eng. (1989) In press] sites; for [Unpublished (1989)
ORNL, PO Box 2009, Oak Ridge, TN 37831].
Draft entry and computer-readable sequence for [Protein Eng. (1989)
In press],[Unpublished (1989) ORNL, PO Box 2009, Oak Ridge, TN
37831] kindly
submitted by F.W.Larimer, 23-OCT-1989.
FEATURES Location/Qualifiers
misc_signal 318..345
/note="tac promoter"
misc_binding 380..384
/note="ribosomal binding site"
rep_origin 942..942
/note="ColE1 origin"
CDS complement(1702..2562)
/codon_start=1702
/product="beta-lactamase"
BASE COUNT 924 a 906 c 963 g 849 t
ORIGIN
1 gaattctggc gaatcctctg accagccaga aaacgacctt tctgtggtga aaccggatgc
61 tgcaattcag agcgccagca agtgggggac agcagaagac ctgaccgccg cagagtggat
121 gtttgacatg gtgaagacta tcgcaccatc agccagaaaa ccgaattttg ctgggtgggc
181 taacgatatc cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct
241 gttccgctgg gcatgccagg acaacttctg gtccggtaac gtgctgagcc cggccaagct
301 tactccccat ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
361 cggataacaa tttcacacag gaggaaggat ccatgggtcg accggccggt caaagcagaa
421 gacgcggagg ctctcccctc cgcgtctttt tttgagcttt ttcgatgggg atccgctgcc
481 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
541 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
601 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
661 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
721 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
841 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
1021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
1081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
1141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
1201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
1261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
1321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
1381 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
1441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
1501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
1561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
1621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
1681 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
1741 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
1801 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
1861 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
1921 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
1981 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
2041 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
2101 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
2161 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
2221 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
2281 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
2341 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
2401 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
2461 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
2521 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
2581 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
2641 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
2701 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
2761 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
2821 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
2881 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
2941 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
3001 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
3061 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
3121 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
3181 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
3241 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
3301 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
3361 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
3421 cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc attcgccatt caggctgcgc
3481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
3541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
3601 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gc
//