GenBank-Updates@genbank.bio.net (01/17/91)
LOCUS BRLAMDA 2447 bp ds-DNA BCT 17-JAN-1991
DEFINITION Brevibacteruim enantiomer-selective amidase (amdA) gene and nitrile
hydratase alpha-subunit, complete cds and 5' end.
ACCESSION M60264 M32282
KEYWORDS enantiomer-selective amidase; nitrile hydratase alpha-subunit.
SOURCE Brevibacterium (strain R312) DNA, clone 717.73.
ORGANISM Brevibacterium sp.
Prokaryota; Bacteria; Firmicutes; Irregular asporogenous rods.
REFERENCE 1 (bases 1 to 2447)
AUTHORS Mayaux,J.-F., Cerbelaud,E., Soubrier,F., Faucher,D. and Petre,D.
TITLE Purification, cloning, and primary structure of an
enantiomer-selective amidase from Brevibacterium sp. strain R312:
Structural evidence for genetic coupling with nitrile hydratase
JOURNAL J. Bacteriol. 172, 6764-6773 (1991)
STANDARD simple staff_review
FEATURES Location/Qualifiers
CDS 245..1810
/gene="amdA"
/evidence=EXPERIMENTAL
/codon_start=245
/product="enantiomer-selective amidase"
CDS 1884..2447
/gene="nitrile hydratase"
/evidence=EXPERIMENTAL
/codon_start=1884
/partial
/product="nitrile hydratase alpha-subunit"
BASE COUNT 529 a 799 c 704 g 415 t
ORIGIN
1 cgatccggaa acagtacttc ggcagcttgc cacgacaccg aaaagctcta cgaacaccgg
61 tgttccactg catcggccga ttctgatcgc tgaatcggcc cgtgggcgac tgtacccccg
121 ctctctctga gcgcacgtaa cccgaactta acgagtcaat atgtcgatac ctattgacgc
181 aattatggat ccggccctag tctgaaagac aagtgaagcc gatcacatca ggagcacact
241 tctcatggcg acaatccgac ctgacgacaa agcaatagac gccgccgcaa ggcattacgg
301 catcactctc gacaaaacag cccggctcga gtggccggca ctgatcgacg gagcactggg
361 ctcctacgac gtcgtcgacc agttgtacgc cgacgaggcg accccgccga ccacgtcacg
421 cgagcacgcg gtgccaagtg cgagcgaaaa tcctttgagc gcttggtatg tgaccaccag
481 catcccgccg acgtcggacg gcgtcctgac cggccgacgc gtggcgatca aggacaacgt
541 gaccgtggcc ggagttccga tgatgaacgg atctcggacg gtagagggat ttactccgtc
601 acgcgacgcg actgtggtca ctcgactact ggcggccggt gcaaccgtcg cgggcaaagc
661 tgtgtgtgag gacctgtgtt tctccggttc gagcttcaca ccggcaagcg gaccggtccg
721 caatccatgg gaccggcagc gcgaagcagg tggatcatcc ggcggcagtg cagcactcgt
781 cgcaaacggt gacgtcgatt ttgccatcgg cggggatcaa ggcggatcga tccggatccc
841 ggcggcattc tgcggcgtcg tcgggcacaa gccgacgttc gggctcgtcc cgtataccgg
901 tgcatttccc atcgagcgaa caatcgacca tctcggcccg atcacacgca cggtccacga
961 tgcagcactg atgctctcgg tcatcgccgg ccgcgacggt aacgacccac gccaagccga
1021 cagtgtcgaa gcaggtgact atctgtccac cctcgactcc gatgtggacg gcctgcgaat
1081 cggaatcgtt cgagagggat tcgggcacgc ggtctcacag cccgaggtcg acgacgcagt
1141 ccgcgcagcg gcacacagtc tgaccgaaat cggttgcacg gtagaggaag taaacatccc
1201 gtggcatctg catgctttcc acatctggaa cgtgatcgcc acggacggtg gtgcctacca
1261 gatgttggac ggcaacggat acggcatgaa cgccgaaggt ttgtacgatc cggaactgat
1321 ggcacacttt gcttctcgac gcattcagca cgccgacgct ctgtccgaaa ccgtcaaact
1381 ggtggccctg accggccacc acggcatcac caccctcggc ggcgcgagct acggcaaagc
1441 ccggaacctc gtaccgcttg cccgcgccgc ctacgacact gccttgagac aattcgacgt
1501 cctggtgatg ccaacgctgc cctacgtcgc atccgaattg ccggcgaagg acgtagatcg
1561 tgcaaccttc atcaccaagg ctctcgggat gatcgccaac acggcaccat tcgacgtgac
1621 cggacatccg tccctgtccg ttccggccgg cctggtgaac gggcttccgg tcggaatgat
1681 gatcaccggc agacacttcg acgatgcgac agtccttcgt gtcggacgcg cattcgaaaa
1741 gcttcgcggc gcgtttccga cgccggccga acgcgcctcc aactctgcac cacaactcag
1801 ccccgcctag tcctgacgca ctgtcagaca acaaattcca ccgattcaca catgatcagc
1861 ccacataaga aaaggtgaac cagatgtcag taacgatcga ccacacaacg gagaacgccg
1921 caccggccca ggcgccggtc tccgaccggg cgtgggcact gttccgcgca ctcgacggta
1981 agggattggt acccgacggt tacgtcgagg gatggaagaa gaccttcgag gaggacttca
2041 gtccaaggcg cggagcggaa ttggtagcgc gcgcatggac cgaccccgag ttccggcagc
2101 tgcttctcac cgacggtacc gccgcagttg cccagtacgg atacctgggc ccccagggcg
2161 aatacatcgt ggcagtcgaa gacaccccga cactcaagaa cgtgatcgtg tgctcgctgt
2221 gttcatgcac cgcgtggccc atcctcggtc tgccacccac ctggtacaag agcttcgaat
2281 accgtgcgcg cgtggtccgc gaaccacgga aggttctctc cgagatggga accgagatcg
2341 cgtcggacat cgagattcgc gtctacgaca ccaccgccga aactcgctac atggtcctcc
2401 cgcagcgtcc cgccggcacc gaaggctgga gccaggaaca actgcag
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