GenBank-Updates@genbank.bio.net (01/17/91)
LOCUS BRLAMDA 2447 bp ds-DNA BCT 17-JAN-1991 DEFINITION Brevibacteruim enantiomer-selective amidase (amdA) gene and nitrile hydratase alpha-subunit, complete cds and 5' end. ACCESSION M60264 M32282 KEYWORDS enantiomer-selective amidase; nitrile hydratase alpha-subunit. SOURCE Brevibacterium (strain R312) DNA, clone 717.73. ORGANISM Brevibacterium sp. Prokaryota; Bacteria; Firmicutes; Irregular asporogenous rods. REFERENCE 1 (bases 1 to 2447) AUTHORS Mayaux,J.-F., Cerbelaud,E., Soubrier,F., Faucher,D. and Petre,D. TITLE Purification, cloning, and primary structure of an enantiomer-selective amidase from Brevibacterium sp. strain R312: Structural evidence for genetic coupling with nitrile hydratase JOURNAL J. Bacteriol. 172, 6764-6773 (1991) STANDARD simple staff_review FEATURES Location/Qualifiers CDS 245..1810 /gene="amdA" /evidence=EXPERIMENTAL /codon_start=245 /product="enantiomer-selective amidase" CDS 1884..2447 /gene="nitrile hydratase" /evidence=EXPERIMENTAL /codon_start=1884 /partial /product="nitrile hydratase alpha-subunit" BASE COUNT 529 a 799 c 704 g 415 t ORIGIN 1 cgatccggaa acagtacttc ggcagcttgc cacgacaccg aaaagctcta cgaacaccgg 61 tgttccactg catcggccga ttctgatcgc tgaatcggcc cgtgggcgac tgtacccccg 121 ctctctctga gcgcacgtaa cccgaactta acgagtcaat atgtcgatac ctattgacgc 181 aattatggat ccggccctag tctgaaagac aagtgaagcc gatcacatca ggagcacact 241 tctcatggcg acaatccgac ctgacgacaa agcaatagac gccgccgcaa ggcattacgg 301 catcactctc gacaaaacag cccggctcga gtggccggca ctgatcgacg gagcactggg 361 ctcctacgac gtcgtcgacc agttgtacgc cgacgaggcg accccgccga ccacgtcacg 421 cgagcacgcg gtgccaagtg cgagcgaaaa tcctttgagc gcttggtatg tgaccaccag 481 catcccgccg acgtcggacg gcgtcctgac cggccgacgc gtggcgatca aggacaacgt 541 gaccgtggcc ggagttccga tgatgaacgg atctcggacg gtagagggat ttactccgtc 601 acgcgacgcg actgtggtca ctcgactact ggcggccggt gcaaccgtcg cgggcaaagc 661 tgtgtgtgag gacctgtgtt tctccggttc gagcttcaca ccggcaagcg gaccggtccg 721 caatccatgg gaccggcagc gcgaagcagg tggatcatcc ggcggcagtg cagcactcgt 781 cgcaaacggt gacgtcgatt ttgccatcgg cggggatcaa ggcggatcga tccggatccc 841 ggcggcattc tgcggcgtcg tcgggcacaa gccgacgttc gggctcgtcc cgtataccgg 901 tgcatttccc atcgagcgaa caatcgacca tctcggcccg atcacacgca cggtccacga 961 tgcagcactg atgctctcgg tcatcgccgg ccgcgacggt aacgacccac gccaagccga 1021 cagtgtcgaa gcaggtgact atctgtccac cctcgactcc gatgtggacg gcctgcgaat 1081 cggaatcgtt cgagagggat tcgggcacgc ggtctcacag cccgaggtcg acgacgcagt 1141 ccgcgcagcg gcacacagtc tgaccgaaat cggttgcacg gtagaggaag taaacatccc 1201 gtggcatctg catgctttcc acatctggaa cgtgatcgcc acggacggtg gtgcctacca 1261 gatgttggac ggcaacggat acggcatgaa cgccgaaggt ttgtacgatc cggaactgat 1321 ggcacacttt gcttctcgac gcattcagca cgccgacgct ctgtccgaaa ccgtcaaact 1381 ggtggccctg accggccacc acggcatcac caccctcggc ggcgcgagct acggcaaagc 1441 ccggaacctc gtaccgcttg cccgcgccgc ctacgacact gccttgagac aattcgacgt 1501 cctggtgatg ccaacgctgc cctacgtcgc atccgaattg ccggcgaagg acgtagatcg 1561 tgcaaccttc atcaccaagg ctctcgggat gatcgccaac acggcaccat tcgacgtgac 1621 cggacatccg tccctgtccg ttccggccgg cctggtgaac gggcttccgg tcggaatgat 1681 gatcaccggc agacacttcg acgatgcgac agtccttcgt gtcggacgcg cattcgaaaa 1741 gcttcgcggc gcgtttccga cgccggccga acgcgcctcc aactctgcac cacaactcag 1801 ccccgcctag tcctgacgca ctgtcagaca acaaattcca ccgattcaca catgatcagc 1861 ccacataaga aaaggtgaac cagatgtcag taacgatcga ccacacaacg gagaacgccg 1921 caccggccca ggcgccggtc tccgaccggg cgtgggcact gttccgcgca ctcgacggta 1981 agggattggt acccgacggt tacgtcgagg gatggaagaa gaccttcgag gaggacttca 2041 gtccaaggcg cggagcggaa ttggtagcgc gcgcatggac cgaccccgag ttccggcagc 2101 tgcttctcac cgacggtacc gccgcagttg cccagtacgg atacctgggc ccccagggcg 2161 aatacatcgt ggcagtcgaa gacaccccga cactcaagaa cgtgatcgtg tgctcgctgt 2221 gttcatgcac cgcgtggccc atcctcggtc tgccacccac ctggtacaag agcttcgaat 2281 accgtgcgcg cgtggtccgc gaaccacgga aggttctctc cgagatggga accgagatcg 2341 cgtcggacat cgagattcgc gtctacgaca ccaccgccga aactcgctac atggtcctcc 2401 cgcagcgtcc cgccggcacc gaaggctgga gccaggaaca actgcag //