GenBank-Updates@genbank.bio.net (01/19/91)
LOCUS ECOTRAX 1413 bp ds-DNA BCT 19-JAN-1991
DEFINITION lasmid F (from Escherichia coli K-12) conjugative transfer
co-repressor (finO) and traX genes, complete cds, and DNA helicase
(traI), 3' end.
ACCESSION M38047
KEYWORDS conjugative transfer co-repressor; traX gene.
SOURCE Plasmid F (from E.coli K12) DNA.
ORGANISM Plasmid F
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1413)
AUTHORS Cram,D.S., Loh,S.M., Cheah,K.C. and Skurray,R.A.
TITLE Nucleotide sequence and evolution of the distal end of the transfer
region on plasmids F and R6-5: Identification of new open reading
frames
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by S.M.Loh, 28-AUG-1990.
Monash University
Dept Microbiology
Wellington Road
Clayton, Melbourne
Victoria, Australia 3168
FEATURES Location/Qualifiers
CDS 1..33
/gene="traI"
/codon_start=1
/partial
/product="DNA helicase"
RBS 35..43
/gene="traX"
CDS 53..799
/gene="traX"
/codon_start=53
/product="TraX protein"
RBS 834..842
/gene="finO"
CDS 853..1413
/note="This gene has been inactivated by the insertion of
IS3 between nt 1248 and 1249"
/gene="finO"
/codon_start=853
/product="conjugative transfer co-repressor"
misc_signal 1246..1248
/note="IS3 insertion target sequence"
BASE COUNT 303 a 357 c 449 g 304 t
ORIGIN
1 ctgcagaaag agaaaaccct gggtggagac tgatacagga agacaaacgc tgatgacaac
61 ggataacacg aacacgacac gtaacgattc actggttgcc cggaccgata cctggttgca
121 gtcgttgctg gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cactggtgct
181 gatggttctg gaccatgcaa accgtattct gcatctggac cagtcgtgga tgtttctagc
241 cgggcgcggg gcctttccgc tgtttgccct ggtgtggggg ctgaatctgt cccgtcatgc
301 gcatatccgg caactggcca ttaaccggct gtggggatgg gcagtttttt cccagttctg
361 gtattacttt gccggctttc cctggtatga ggggaatatc ctgtttgcct ttgcagtggc
421 ggcacaggtg ctgacatggt gtgaaacgcg cagtgggtgg cgtactgcag cggccattct
481 catgatggca ctgtgggggc ctttgtccgg caccagttac ggcattgccg ggctgctgat
541 gctggctgtc agccaccggc tgtaccgggc ggaagacaga atggaacgtc tggtgcttgt
601 cgcttgcctg ctggccgtga ttccggcgct taaccttgcc accagtgatg cggcggctgt
661 agccggcctt gtgatgacgg tgctgaccgt tggtctggtg tcgtgtgcag ggaaattatt
721 atcccgtttc tggcccggag actttttccc gacgttctat gcctgtcatc tggctgtgct
781 gggcgttctg gcgctgtgac gggtgtggta tctttggccg caagaggtga ttcgtcagag
841 gcagaacaca gcatgacaga gcagaagcga ccggtactga cactgaagcg gaagacagaa
901 ggggagacgc ttgtccgcag ccggaaaacc atcatcaatg tcaccacgcc accaaagtgg
961 aaggtgaaaa agcagaagct ggccgagaaa gccgcccggg aagcagagct ggcggcaaaa
1021 aaagcgcagg ccagacaggc gctgtccatt tatctgaatc tgcccacgct ggatgatgcc
1081 gtgaacaccc tgaagccctg gtggccggga ttatttgacg gtgacacgcc ccggcttctg
1141 gcctgcggta tccgggacgt gttactggaa gacgtggcgc agcggaatat cccgctctcg
1201 cataaaaaac tgcgcagggc gctgaaggcc atcacccgtt cagaaagcta tctgtgtgcc
1261 atgaaagccg gtgcctgccg gtatgacacg gaagggtatg tgacggagca tatttctcag
1321 gaggaggaag cgtatgcggc agagcgtctg gataaaatcc gccgccagaa ccggataaag
1381 gcagaacttc aggccgtgct tgatgagaaa taa
//