GenBank-Updates@genbank.bio.net (01/19/91)
LOCUS HUMGRFP1 474 bp ds-DNA PRI 19-JAN-1991 DEFINITION Human growth hormone-releasing factor (GRF) gene, exon 1 (complete cds in 5 segments). ACCESSION N00013 K00645 K00646 KEYWORDS growth hormone; growth hormone-releasing factor; hormone; preprosomatocrinin. SEGMENT 1 of 5 SOURCE Human: pancreatic tumor, cDNA to mRNA, clones 8 and 21 [Proc. Natl. Acad. Sci. U.S.A. 80, 4311-4314 (1983)]; genomic DNA, clones hGRF-lambda-[101,111] and hGRFcos49 [Proc. Natl. Acad. Sci. U.S.A. 82, 63-67 (1985)]. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 372 to 464) AUTHORS Gubler,U., Monahan,J.J., Lomedico,P.T., Bhatt,R.S., Collier,K.J., Hoffman,B.J., Boehlen,P., Esch,F., Ling,N., Zeytin,F., Brazeau,P., Poonian,M.S. and Gage,L.P. TITLE Cloning and sequence analysis of cDNA for the precursor of human growth hormone-releasing factor JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80, 4311-4314 (1983) STANDARD full staff_review REFERENCE 2 (bases 1 to 474) AUTHORS Mayo,K.E., Cerelli,G.M., Lebo,R.V., Bruce,B.D., Rosenfeld,M.G. and Evans,R.M. TITLE Gene encoding human growth hormone-releasing factor precursor: Structure, sequence, and chromosomal assignment JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82, 63-67 (1985) STANDARD full staff_review COMMENT [Proc. Natl. Acad. Sci. U.S.A. 82, 63-67 (1985)] notes the following consensus sequences: a CAAT box (318-325) and a TATA box (363-369). [Proc. Natl. Acad. Sci. U.S.A. 80, 4311-4314 (1983)] reports two cDNA sequences, PPGRF- 107 and PPGRF-108. The two are very homologous: PPGRF-108 has an additional 21 bp of mRNA 5' to the start of exon 1, contains an extra serine codon at the beginning of exon 5, and has an extra 7 bp just prior to the poly-A site relative to PPGRF-107. The first 20 amino acids represent a possible hydrophobic signal. FEATURES Location/Qualifiers precursor_RNA 393..>474 /note="GRF-108 mRNA (5' end +/- 1 bp)[Proc. Natl. Acad. Sci. U.S.A. 82, 63-67 (1985)] /nomgen='GHRF' /map='20p11.23-qter' /hgml_locus_uid='LM0006U'" precursor_RNA 393..>474 /note="GRF-107 mRNA (5' end +/- 1 bp)[Proc. Natl. Acad. Sci. U.S.A. 82, 63-67 (1985)]" intron 465..>474 /note="GRF-107 mRNA intron A" BASE COUNT 124 a 114 c 120 g 116 t ORIGIN 1 bp upstream of XbaI site on chromosome 20. 1 tctagacagg gtctcattat gttgccaggt tggtctcaaa cttctgagct caggcaatcc 61 acccgcctca gcctcccaaa gtgctaggat ggcaggtgtg agccaccgcg cccagccgag 121 ttctccaatc actattatag cagtatatat tctctatatc ctcttggaat aatgttacac 181 ctttgtacta tgtccactgt gccaaagata aaaggagact ttactaggag tctaagtctg 241 caaggggcca aacctctttc accaacaggg tttgtcagtg tgatatgatg ctaaaaacag 301 tcctttggtt gacttgtggg taattgattc tctgacgctg acaacgctta ggaaaatgaa 361 gagataaatg atgggaacgc caggcggctg ccagagcaaa cacccagccc agggcccctg 421 gatttgagca gtgcctcgga gcagagggat atctgccgca tcaggtgaga gggg //