GenBank-Updates@genbank.bio.net (01/19/91)
LOCUS HUMGRFP1 474 bp ds-DNA PRI 19-JAN-1991
DEFINITION Human growth hormone-releasing factor (GRF) gene, exon 1 (complete
cds in 5 segments).
ACCESSION N00013 K00645 K00646
KEYWORDS growth hormone; growth hormone-releasing factor; hormone;
preprosomatocrinin.
SEGMENT 1 of 5
SOURCE Human: pancreatic tumor, cDNA to mRNA, clones 8 and 21 [Proc. Natl.
Acad. Sci. U.S.A. 80, 4311-4314 (1983)]; genomic DNA, clones
hGRF-lambda-[101,111] and hGRFcos49 [Proc. Natl. Acad. Sci. U.S.A.
82, 63-67 (1985)].
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 372 to 464)
AUTHORS Gubler,U., Monahan,J.J., Lomedico,P.T., Bhatt,R.S., Collier,K.J.,
Hoffman,B.J., Boehlen,P., Esch,F., Ling,N., Zeytin,F., Brazeau,P.,
Poonian,M.S. and Gage,L.P.
TITLE Cloning and sequence analysis of cDNA for the precursor of human
growth hormone-releasing factor
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80, 4311-4314 (1983)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 474)
AUTHORS Mayo,K.E., Cerelli,G.M., Lebo,R.V., Bruce,B.D., Rosenfeld,M.G. and
Evans,R.M.
TITLE Gene encoding human growth hormone-releasing factor precursor:
Structure, sequence, and chromosomal assignment
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82, 63-67 (1985)
STANDARD full staff_review
COMMENT [Proc. Natl. Acad. Sci. U.S.A. 82, 63-67 (1985)] notes the
following consensus sequences: a CAAT box (318-325)
and a TATA box (363-369). [Proc. Natl. Acad. Sci. U.S.A. 80,
4311-4314 (1983)] reports two cDNA sequences, PPGRF-
107 and PPGRF-108. The two are very homologous: PPGRF-108 has an
additional 21 bp of mRNA 5' to the start of exon 1, contains an
extra serine codon at the beginning of exon 5, and has an extra 7
bp just prior to the poly-A site relative to PPGRF-107. The first
20 amino acids represent a possible hydrophobic signal.
FEATURES Location/Qualifiers
precursor_RNA 393..>474
/note="GRF-108 mRNA (5' end +/- 1 bp)[Proc. Natl. Acad.
Sci. U.S.A. 82, 63-67 (1985)] /nomgen='GHRF'
/map='20p11.23-qter' /hgml_locus_uid='LM0006U'"
precursor_RNA 393..>474
/note="GRF-107 mRNA (5' end +/- 1 bp)[Proc. Natl. Acad.
Sci. U.S.A. 82, 63-67 (1985)]"
intron 465..>474
/note="GRF-107 mRNA intron A"
BASE COUNT 124 a 114 c 120 g 116 t
ORIGIN 1 bp upstream of XbaI site on chromosome 20.
1 tctagacagg gtctcattat gttgccaggt tggtctcaaa cttctgagct caggcaatcc
61 acccgcctca gcctcccaaa gtgctaggat ggcaggtgtg agccaccgcg cccagccgag
121 ttctccaatc actattatag cagtatatat tctctatatc ctcttggaat aatgttacac
181 ctttgtacta tgtccactgt gccaaagata aaaggagact ttactaggag tctaagtctg
241 caaggggcca aacctctttc accaacaggg tttgtcagtg tgatatgatg ctaaaaacag
301 tcctttggtt gacttgtggg taattgattc tctgacgctg acaacgctta ggaaaatgaa
361 gagataaatg atgggaacgc caggcggctg ccagagcaaa cacccagccc agggcccctg
421 gatttgagca gtgcctcgga gcagagggat atctgccgca tcaggtgaga gggg
//