GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS ANANIF 5933 bp ds-DNA PRI 16-JAN-1991
DEFINITION Anabaena sp. nitrogen fixation-associated protein genes ORF1-ORF4,
complete cds.
ACCESSION M38044
KEYWORDS nitrogen fixation protein.
SOURCE Anabeana sp. (strain Pasteur culture collection 7120) DNA, clone
pAN207.
ORGANISM Anabaena sp.
Prokaryota; Bacteria; Gracilicutes; Oxyphotobacteria;
Cyanobacteria; Nostocales; Nostocaceae.
REFERENCE 1 (sites)
AUTHORS Lammers,P.J., McLaughlin,S., Papin,S., Trujillo-Provencio,C. and
Ryncarz,A.J.III.
TITLE Developmental rearrangement of cyanobacterial nif genes: Nucleotide
sequence, open reading frames and cytochrome P450 homology from the
Anabaena 7120 nifD element
JOURNAL Unpublished , 0-0 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by P.J.Lammers, 28-AUG-1990.
New Mexico State University
Box 30001 Dept. 3C
Las Cruces, NM 88003
FEATURES Location/Qualifiers
CDS 220..1185
/note="ORF1"
/codon_start=220
/product="nitrogen fixation-assocated protein"
CDS 1631..2125
/note="ORF2"
/codon_start=1631
/product="nitrogen fixation-assocated protein"
CDS 2468..3532
/note="ORF3"
/codon_start=2468
/product="nitrogen fixation-assocated protein"
CDS 4020..4136
/note="ORF4"
/codon_start=4020
/product="nitrogen fixation-assocated protein"
BASE COUNT 1931 a 1186 c 1117 g 1699 t
ORIGIN
1 aagcttaagc atttatactg acaaaaacgc ctttaattta aaaatatgca aatacgcttt
61 tttgctacca accgcgatcg ccaaaatcta ggtctcaatg ttgaccgtga tacacgcatt
121 aaattactaa aatttggtta tcactgggtt gatatgaaaa aatatatggc tcactatttg
181 gcgactactg atccttcaac tatgccacca ggagtaatta tggaagattc tgaggagacg
241 gtatttaaca agttctcaaa aagaggcgta aacacattat cattggcact catggttaca
301 tgtcctttca tgggcattaa ctcttttcga tttggcagac cccttaaggg agccttaaaa
361 aagcataatt ttactttgat tgctgatcca gaagaaaaga tagatatcaa ctccaaccaa
421 aatttaatcg cttttgtcgg attttcctgg ccttccaatg gcaaagtttt agattacaat
481 tcggatcgta ctgagtgtgt acaatctgcc ccagccttag ctaatctgat cagttatatt
541 cgcactaaaa aacctgacat taaaatctac gtcatagctc acagtatggg cagttacctt
601 gtttgccatt atgtggaaca gctagttaac caagcctttg agccaacaga attgaatgag
661 gagattaaaa acagattaaa acgaaaagat agaggcggag aaaatacttt ttttgtcgat
721 cgctatttta tgttagctcc tgatgtggaa cgtcgggaag tgaccaagtg tgatctaggc
781 ggatctgaat atacaggccc gttttactct ggtctggaac acctggttca agaaagtcat
841 gtattctact ctcgctacga taatgcacta aaagcctcag tagttgaaaa agacgcaatt
901 cgtgagtcat tgcagaaagg attcgagtta ttcaccggcc ccgacttgca aaaacgttgg
961 gagagcagcc ttggtctcaa ccccttacca gctttagctc ccaataatgt ctacagtcat
1021 aatgcaacag tgttgactaa tcgacaaatc gaccatggag actatttcga tgctcccgca
1081 atcatagatc aaatcgccaa tatcatatct gaagcaaata ctagtcgcat tcctgagctt
1141 ccctggcgtt tttctgagtc aagcgatcgc cctctaatag agtaaaattt gagatgcaaa
1201 aattaacgtc ggctcaatag ctcatggcga tgaccttcgg ctgtcgggga ctatcgcatc
1261 agagataatt aatcatgtct gcatacataa cttaaaagct catgaaaaca tggttccggt
1321 atgtgttgat atcgaatgca atcttcaatt tgtttccagc agtcatgttt cgaattcttt
1381 tgcgatcact ttatctactg ggctatgctg tttcaaacca gtcaaaaaac ttacgtatcg
1441 ccaaatagac aatttgctac tattaaaaaa attgagaatt gcctacaatc caaattaggt
1501 aataaactcg atcggagacc atcgccagaa tagcagtttg caaaaacact atggcaaaac
1561 gcaagaaaag caatctccag tggattaaag aaactctcga cttaaaacca gacatcgttg
1621 ggaagcgcag atggctacga atattgtagc taatcgcggg gctgttcgct tcaacgtccc
1681 tcaagactgg ttttttgagc cacaggaaaa atccttcaaa ttcagcgata aaaaaccacc
1741 caaagatgat tgcgcgttgg aagtgtcttt taatcaactt cctcccaacg actggactct
1801 gtttcccctc aaatccacct tgaggaaagt agtagaagac gacggccgtg atgtgattga
1861 gaaaggagaa atcattagcc tcaagcgcca aactgccaaa attgtctgga cagaactcaa
1921 atttatcgat acccaagaag aaccacggga agctttttcg cggatttgca ttggtttggg
1981 gtcaaatgtg caatgtttga ttacattcga gttttgggct gatcaagcag agcaaatgac
2041 acctgtttgg gatgaagtca tgcgatcgct tacactaggt ctgtatatcc gtgacccaag
2101 cactggtttg gctttccccg actgagccac agttaataat cagggtaaat attatgttga
2161 ctcaattacc aaatcctatt agtgtccctt catggtggca acttatcaat tggattgctg
2221 atccgattgg atttcagaaa aaatatagta aaaagtatgg caacattttt tccatgacgc
2281 ttctggaatc ggctctttcg aattttaggg gagccacaag ctcttcaaga aattttcacc
2341 caagattcta gatttgatgt tggtcgggga aatacacttg cagagcctct cattgggcga
2401 acttctctta tgttaatgga tggcgatcgc gatcgacgag aacgaaaatt attaatgccc
2461 ccctttcatg gagaaaggca tacaagctta tgctcaacaa atctgcttaa ttaccaacca
2521 gatcgccagc gaatggcaaa ttgggcaacc ttttgttgct aggtctgcca tgcagaaact
2581 gagtctagag gtgattatac aaattgtctt tggtttagct gacggagaac gctatcaaca
2641 aataaagcct ctattcacag attggctgaa tatgactgat tctcccttac gctccagtat
2701 gctttttcta aagtccctac aaaaagattg gggaacctgg actccttggg gacagatgaa
2761 acataaacaa cgttctattt atgatctact ccaagcagaa atagaagaga aaagaacaaa
2821 ggaaaatgag cagcgcggtg atgttctaag tttgatgatg gcagcacgag atgaaaatgg
2881 gcaagccatg acagacgagg aactaaaaga tgaattgcta acaattttat ttgctggaca
2941 tgaaactacc gcaaccacaa tagcgtgggc tttctatcaa attttaaaaa atgtaaatgt
3001 gcaggaaaaa ttacagcagg aactcgacag attgggagca aaccctaacc caatggaaat
3061 tgcccagctt ccttacctaa cagcagtttc tcaagaaact ctgcggatgt atccagtctt
3121 accaacactt tttccacgca ttaccaaatc atctataaat attgccggat accaattgga
3181 acctgacaca accttaatgg cgagtatata cctcatacat taccgggaag acttgtatcc
3241 taatcctcaa caatttcgac cagaacgttt tatagaacgg caatattctc cttcagaata
3301 cattcctttt ggtggtggaa gtcgtcgctg tttgggtata gctctagctc tgttagaaat
3361 aaaattggtt attgcaacag ttctatcaaa ttatcaactt gctctagcgg aagataaacc
3421 tgttaacgtg caacgacgtg gatttaccct ggctccagat ggtggagtta gggtaataat
3481 gactggcaaa aaatcattga aatttgagca aagtagcaag attttcaact gaagatttgt
3541 cggtatttca tattgagctt atttagagcg tcaaggcgaa ttaagttggt attgattagc
3601 ccatagaata ctaattattg agcatataga tacaataact tatgactcta tctaaatctt
3661 cctgctgttc ggagactttc cacaacagaa aaactcaagc agacttcgca gcagtcaagt
3721 tagtgtctgc atgggtgcgt agtccgaatg cacctctaat tcttggtcaa accaactttt
3781 ttattgagtt cgacgtttgc ttttaccgtt cccagtttga gtttgaagta caaccaaagt
3841 cctcaacagc aagctaacaa tcattgggct cggactaatt tgcgatcgcc atcactgtgt
3901 cttttatacc ttggcgtacg ttaccatagc gatcgcgtag ctaatcaagg taattgcatt
3961 ctttttatga cgataagata aattatcagt cacgccatta gagttaatag gagcgcaaaa
4021 tgacggagaa tgatcaggca atcagccaaa tacgtcaaat tcgccacatg atatctgagg
4081 aaaatgaaca caaccctcag aaactggtga attactacat ccaactccag aagtaatatc
4141 ctcacctcat acaatataat ccacaggaca ggaactttta aaatcgtcac cacaaaaagg
4201 tattgaagcc taaatacgca agtaatctgt aaagtcttgc ccaaggtttg gttaaaaaat
4261 tttattgcca ttagcgtagt ttactgtagc gatcgcctag ctaatcaaga taattgcatt
4321 ctgttgatga cgataagata aattatcagt gatgggaaca actacttaag cttaccgtag
4381 gtatcgctaa ctttagccat tagtgcgtta gcggagctta ccgtaggtat cgccagccca
4441 tcaagctaat tcaatcatat aaaaatccaa ggttttttat gtgcttacat aaatgcgctt
4501 cacaaatttt atctaaaata tgaatgtatt atttaatact ttattttttt ggctataact
4561 ttagctaatg ctcgcaagaa attctatata atctagattt gtgtttaact tgtttacgta
4621 ataatactac tacaacaatc ttaaattggt gctaaattaa aacaatactt taatactact
4681 gagaggcagt tgttagtgat accagaaggg acagtggttg tggaagcaaa cgtcagactg
4741 actgtcaaaa tcttaaaaga tggcgatcag gttttaaatg tctggtactg caattcaagt
4801 tattttgtcg catccgaaaa taatggagag gtttgtgtga tttgtaacct tagatgaaga
4861 aaagattcca agactaagca aaactccgcc tttgaaagtc acatttggtg aaggtgaggt
4921 agaagccatt ataagttcag aagatggaga tattaaagta acgactgact aagcagaact
4981 tcaaacacaa atatattatg gcaaaaaatt cagacagaaa tcgagctatt aacgcaaaaa
5041 ttgacaattt gactgctgaa gagcaagaaa aactcagcga cgctataagg aaagctaaaa
5101 gaatatgctc caaatgccag atccacagtt gttgaatacg agcaaggtaa gttaccttct
5161 tctcaatctc aaaatcaact tgaaggacag aacgattaaa gtaagaattt gagaaataac
5221 attttatgac acaaaaaaga ccttacgaac atcgaaaagc acaaaaacaa gtcaagaatt
5281 tagaatctta tcaatgtatg gtttgctggg aggttaactc caaagctaat ggtcatcact
5341 tagttcccta tagtgaagga ggttctgctg atattcaaaa catgatgact ctttgtcctt
5401 catgtcatac aagtatcata aaggtgaact aaaaatcgat attcatggat tttgatattg
5461 actaacaaag ctcagtatct taagtttatt attcgcaatt tatatcaata gttgtgttga
5521 agagactcac aactctattg tagaggatgt ttttagcatc ctctaatttt acatttcttt
5581 tctatccttg aataacttta attattttct atatagtagc tggaatgtag attttctgtc
5641 gcatacctca tcgcatactt cctagtctta gtgattcaaa gcgatcgcct aaccgaccca
5701 cattaatcaa ataataacaa ttaaatagtc ttttatttat ataccccaaa tctaaccaaa
5761 acccaaaaac cagacccaat aacagttata ttgtcttaag ccgcaaaaat gtattgttcc
5821 cagataaccc tacaacctgt atttaggcta aaaatagact cttttttctc ccctcacaaa
5881 aaaaccttac ctcatctgac atctgcaccc cgtcgccaag ttcggagtaa tcc
//GenBank-Updates@genbank.bio.net (01/19/91)
LOCUS ANANIF 5933 bp ds-DNA PRI 19-JAN-1991
DEFINITION Anabaena sp. nitrogen fixation-associated protein genes ORF1-ORF4,
complete cds.
ACCESSION M38044
KEYWORDS nitrogen fixation protein.
SOURCE Anabeana sp. (strain Pasteur culture collection 7120) DNA, clone
pAN207.
ORGANISM Anabaena sp.
Prokaryota; Bacteria; Gracilicutes; Oxyphotobacteria;
Cyanobacteria; Nostocales; Nostocaceae.
REFERENCE 1 (sites)
AUTHORS Lammers,P.J., McLaughlin,S., Papin,S., Trujillo-Provencio,C. and
Ryncarz,A.J.II.
TITLE Developmental rearrangement of cyanobacterial nif genes: nucleotide
sequence, open reading frames, and cytochrome p-450 homology of the
Anabaena sp. strain PCC 7120 nifD element
JOURNAL J. Bacteriol. 172, 6981-6990 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 220..1185
/note="ORF1"
/codon_start=220
/product="nitrogen fixation-assocated protein"
CDS 1631..2125
/note="ORF2"
/codon_start=1631
/product="nitrogen fixation-assocated protein"
CDS 2468..3532
/note="ORF3"
/codon_start=2468
/product="nitrogen fixation-assocated protein"
CDS 4020..4136
/note="ORF4"
/codon_start=4020
/product="nitrogen fixation-assocated protein"
BASE COUNT 1931 a 1186 c 1117 g 1699 t
ORIGIN
1 aagcttaagc atttatactg acaaaaacgc ctttaattta aaaatatgca aatacgcttt
61 tttgctacca accgcgatcg ccaaaatcta ggtctcaatg ttgaccgtga tacacgcatt
121 aaattactaa aatttggtta tcactgggtt gatatgaaaa aatatatggc tcactatttg
181 gcgactactg atccttcaac tatgccacca ggagtaatta tggaagattc tgaggagacg
241 gtatttaaca agttctcaaa aagaggcgta aacacattat cattggcact catggttaca
301 tgtcctttca tgggcattaa ctcttttcga tttggcagac cccttaaggg agccttaaaa
361 aagcataatt ttactttgat tgctgatcca gaagaaaaga tagatatcaa ctccaaccaa
421 aatttaatcg cttttgtcgg attttcctgg ccttccaatg gcaaagtttt agattacaat
481 tcggatcgta ctgagtgtgt acaatctgcc ccagccttag ctaatctgat cagttatatt
541 cgcactaaaa aacctgacat taaaatctac gtcatagctc acagtatggg cagttacctt
601 gtttgccatt atgtggaaca gctagttaac caagcctttg agccaacaga attgaatgag
661 gagattaaaa acagattaaa acgaaaagat agaggcggag aaaatacttt ttttgtcgat
721 cgctatttta tgttagctcc tgatgtggaa cgtcgggaag tgaccaagtg tgatctaggc
781 ggatctgaat atacaggccc gttttactct ggtctggaac acctggttca agaaagtcat
841 gtattctact ctcgctacga taatgcacta aaagcctcag tagttgaaaa agacgcaatt
901 cgtgagtcat tgcagaaagg attcgagtta ttcaccggcc ccgacttgca aaaacgttgg
961 gagagcagcc ttggtctcaa ccccttacca gctttagctc ccaataatgt ctacagtcat
1021 aatgcaacag tgttgactaa tcgacaaatc gaccatggag actatttcga tgctcccgca
1081 atcatagatc aaatcgccaa tatcatatct gaagcaaata ctagtcgcat tcctgagctt
1141 ccctggcgtt tttctgagtc aagcgatcgc cctctaatag agtaaaattt gagatgcaaa
1201 aattaacgtc ggctcaatag ctcatggcga tgaccttcgg ctgtcgggga ctatcgcatc
1261 agagataatt aatcatgtct gcatacataa cttaaaagct catgaaaaca tggttccggt
1321 atgtgttgat atcgaatgca atcttcaatt tgtttccagc agtcatgttt cgaattcttt
1381 tgcgatcact ttatctactg ggctatgctg tttcaaacca gtcaaaaaac ttacgtatcg
1441 ccaaatagac aatttgctac tattaaaaaa attgagaatt gcctacaatc caaattaggt
1501 aataaactcg atcggagacc atcgccagaa tagcagtttg caaaaacact atggcaaaac
1561 gcaagaaaag caatctccag tggattaaag aaactctcga cttaaaacca gacatcgttg
1621 ggaagcgcag atggctacga atattgtagc taatcgcggg gctgttcgct tcaacgtccc
1681 tcaagactgg ttttttgagc cacaggaaaa atccttcaaa ttcagcgata aaaaaccacc
1741 caaagatgat tgcgcgttgg aagtgtcttt taatcaactt cctcccaacg actggactct
1801 gtttcccctc aaatccacct tgaggaaagt agtagaagac gacggccgtg atgtgattga
1861 gaaaggagaa atcattagcc tcaagcgcca aactgccaaa attgtctgga cagaactcaa
1921 atttatcgat acccaagaag aaccacggga agctttttcg cggatttgca ttggtttggg
1981 gtcaaatgtg caatgtttga ttacattcga gttttgggct gatcaagcag agcaaatgac
2041 acctgtttgg gatgaagtca tgcgatcgct tacactaggt ctgtatatcc gtgacccaag
2101 cactggtttg gctttccccg actgagccac agttaataat cagggtaaat attatgttga
2161 ctcaattacc aaatcctatt agtgtccctt catggtggca acttatcaat tggattgctg
2221 atccgattgg atttcagaaa aaatatagta aaaagtatgg caacattttt tccatgacgc
2281 ttctggaatc ggctctttcg aattttaggg gagccacaag ctcttcaaga aattttcacc
2341 caagattcta gatttgatgt tggtcgggga aatacacttg cagagcctct cattgggcga
2401 acttctctta tgttaatgga tggcgatcgc gatcgacgag aacgaaaatt attaatgccc
2461 ccctttcatg gagaaaggca tacaagctta tgctcaacaa atctgcttaa ttaccaacca
2521 gatcgccagc gaatggcaaa ttgggcaacc ttttgttgct aggtctgcca tgcagaaact
2581 gagtctagag gtgattatac aaattgtctt tggtttagct gacggagaac gctatcaaca
2641 aataaagcct ctattcacag attggctgaa tatgactgat tctcccttac gctccagtat
2701 gctttttcta aagtccctac aaaaagattg gggaacctgg actccttggg gacagatgaa
2761 acataaacaa cgttctattt atgatctact ccaagcagaa atagaagaga aaagaacaaa
2821 ggaaaatgag cagcgcggtg atgttctaag tttgatgatg gcagcacgag atgaaaatgg
2881 gcaagccatg acagacgagg aactaaaaga tgaattgcta acaattttat ttgctggaca
2941 tgaaactacc gcaaccacaa tagcgtgggc tttctatcaa attttaaaaa atgtaaatgt
3001 gcaggaaaaa ttacagcagg aactcgacag attgggagca aaccctaacc caatggaaat
3061 tgcccagctt ccttacctaa cagcagtttc tcaagaaact ctgcggatgt atccagtctt
3121 accaacactt tttccacgca ttaccaaatc atctataaat attgccggat accaattgga
3181 acctgacaca accttaatgg cgagtatata cctcatacat taccgggaag acttgtatcc
3241 taatcctcaa caatttcgac cagaacgttt tatagaacgg caatattctc cttcagaata
3301 cattcctttt ggtggtggaa gtcgtcgctg tttgggtata gctctagctc tgttagaaat
3361 aaaattggtt attgcaacag ttctatcaaa ttatcaactt gctctagcgg aagataaacc
3421 tgttaacgtg caacgacgtg gatttaccct ggctccagat ggtggagtta gggtaataat
3481 gactggcaaa aaatcattga aatttgagca aagtagcaag attttcaact gaagatttgt
3541 cggtatttca tattgagctt atttagagcg tcaaggcgaa ttaagttggt attgattagc
3601 ccatagaata ctaattattg agcatataga tacaataact tatgactcta tctaaatctt
3661 cctgctgttc ggagactttc cacaacagaa aaactcaagc agacttcgca gcagtcaagt
3721 tagtgtctgc atgggtgcgt agtccgaatg cacctctaat tcttggtcaa accaactttt
3781 ttattgagtt cgacgtttgc ttttaccgtt cccagtttga gtttgaagta caaccaaagt
3841 cctcaacagc aagctaacaa tcattgggct cggactaatt tgcgatcgcc atcactgtgt
3901 cttttatacc ttggcgtacg ttaccatagc gatcgcgtag ctaatcaagg taattgcatt
3961 ctttttatga cgataagata aattatcagt cacgccatta gagttaatag gagcgcaaaa
4021 tgacggagaa tgatcaggca atcagccaaa tacgtcaaat tcgccacatg atatctgagg
4081 aaaatgaaca caaccctcag aaactggtga attactacat ccaactccag aagtaatatc
4141 ctcacctcat acaatataat ccacaggaca ggaactttta aaatcgtcac cacaaaaagg
4201 tattgaagcc taaatacgca agtaatctgt aaagtcttgc ccaaggtttg gttaaaaaat
4261 tttattgcca ttagcgtagt ttactgtagc gatcgcctag ctaatcaaga taattgcatt
4321 ctgttgatga cgataagata aattatcagt gatgggaaca actacttaag cttaccgtag
4381 gtatcgctaa ctttagccat tagtgcgtta gcggagctta ccgtaggtat cgccagccca
4441 tcaagctaat tcaatcatat aaaaatccaa ggttttttat gtgcttacat aaatgcgctt
4501 cacaaatttt atctaaaata tgaatgtatt atttaatact ttattttttt ggctataact
4561 ttagctaatg ctcgcaagaa attctatata atctagattt gtgtttaact tgtttacgta
4621 ataatactac tacaacaatc ttaaattggt gctaaattaa aacaatactt taatactact
4681 gagaggcagt tgttagtgat accagaaggg acagtggttg tggaagcaaa cgtcagactg
4741 actgtcaaaa tcttaaaaga tggcgatcag gttttaaatg tctggtactg caattcaagt
4801 tattttgtcg catccgaaaa taatggagag gtttgtgtga tttgtaacct tagatgaaga
4861 aaagattcca agactaagca aaactccgcc tttgaaagtc acatttggtg aaggtgaggt
4921 agaagccatt ataagttcag aagatggaga tattaaagta acgactgact aagcagaact
4981 tcaaacacaa atatattatg gcaaaaaatt cagacagaaa tcgagctatt aacgcaaaaa
5041 ttgacaattt gactgctgaa gagcaagaaa aactcagcga cgctataagg aaagctaaaa
5101 gaatatgctc caaatgccag atccacagtt gttgaatacg agcaaggtaa gttaccttct
5161 tctcaatctc aaaatcaact tgaaggacag aacgattaaa gtaagaattt gagaaataac
5221 attttatgac acaaaaaaga ccttacgaac atcgaaaagc acaaaaacaa gtcaagaatt
5281 tagaatctta tcaatgtatg gtttgctggg aggttaactc caaagctaat ggtcatcact
5341 tagttcccta tagtgaagga ggttctgctg atattcaaaa catgatgact ctttgtcctt
5401 catgtcatac aagtatcata aaggtgaact aaaaatcgat attcatggat tttgatattg
5461 actaacaaag ctcagtatct taagtttatt attcgcaatt tatatcaata gttgtgttga
5521 agagactcac aactctattg tagaggatgt ttttagcatc ctctaatttt acatttcttt
5581 tctatccttg aataacttta attattttct atatagtagc tggaatgtag attttctgtc
5641 gcatacctca tcgcatactt cctagtctta gtgattcaaa gcgatcgcct aaccgaccca
5701 cattaatcaa ataataacaa ttaaatagtc ttttatttat ataccccaaa tctaaccaaa
5761 acccaaaaac cagacccaat aacagttata ttgtcttaag ccgcaaaaat gtattgttcc
5821 cagataaccc tacaacctgt atttaggcta aaaatagact cttttttctc ccctcacaaa
5881 aaaaccttac ctcatctgac atctgcaccc cgtcgccaag ttcggagtaa tcc
//