GenBank-Updates@genbank.bio.net (01/23/91)
LOCUS CLOADC 4054 bp ds-DNA BCT 23-JAN-1991
DEFINITION Clostridium acetobutylicum acetoacetate decarboxylase gene,
complete cds.
ACCESSION M55392 M34078
KEYWORDS acetoacetate decarboxylase.
SOURCE C.acetobutylicum (strain DSM 792) DNA, clones pUG[67,80].
ORGANISM Clostridium acetobutylicum
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4054)
AUTHORS Gerischer,U. and Duerre,P.
TITLE Cloning, sequencing, and molecular analysis of the acetoacetate
decarboxylase gene region from Clostridium acetobutylicum
JOURNAL J. Bacteriol. 172, 6907-6918 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 1..1410
/note="ORF1"
/codon_start=1
/partial
CDS complement(1450..2004)
/note="ORF2"
/codon_start=1450
RBS complement(2012..2016)
/note="for ORF2"
RBS 2400..2405
/note="for ORF3"
/gene="adc"
CDS 2414..3148
/EC_number="4.1.1.4"
/gene="adc"
/codon_start=2414
/product="acetoacetate decarboxylase"
terminator 3155..3191
/gene="adc"
CDS complement(3214..3879)
/note="ORF3"
/codon_start=3214
RBS complement(3889..3893)
/note="for ORF3"
BASE COUNT 1330 a 609 c 763 g 1352 t
ORIGIN
1 ggtgcaatta aatcaggtga tttaactaat gcgcagggca tggggggact tgatagtaat
61 aagtgtgtgg atatgcttga aacacatgat gagtacgagc ataatgaaag taaagattta
121 acagactggc aaagaaaagc aggttgggca atagctgctt caagagctgg ttcagttccc
181 ttattctttg atagacctac aggaaatata ggaagtgagg gagatgctct ctggaaggat
241 tctgatgttg tagctgttaa tgaatttcat aacgcgatgg caggtcaaaa tgaatattta
301 aggttacaaa ataacaataa ggcaatgatt atagaaagag gaagtaaagg agctgttatt
361 gtaaatgaag gcgacagctt taatttaaac accccaacta atttagagga cggaaattat
421 gataatcatg gtagtgcaac cgattcttta actgtttctc aggggagaat gacaggaaca
481 gtaccagcaa attcaatcat agtgatttac aacaagaata gtaacccagg cagcgacaga
541 gttacattaa gtgagcaagc tgcaaaagca ggagatagtg taacgataac ttatgatgcg
601 ggaacaacag cgctaaagga tgcttcaaat gtaaacttgt actggggtta tgacggtttt
661 agcgctgcaa caagtaaggc aatgacatca cttggagaca ataagtggca gacaactata
721 acagtgccta aagaggttac taagaatgtt aatttttctt ttacagatgg gacaagttgg
781 gataataaca atggagctaa ttggaatata ccattagcca gtaattacct tccacatgga
841 ggctataaag ttgattatga cagcagcaac ctagtttcgg gtaataattt tacaatttac
901 tataatggaa acttagcgaa ttcatcaaac gtatccttgc actggggagt taacggatgg
961 agtaatatgc aaaacttagc tatggtaaag gattcaaatg gtttttggga ggctactata
1021 gcgatacctg cttcatcaaa tacattgaat ttttgtttca caaatggaag cagttgggat
1081 aataataaca acaataattg gactttaaat acatggagca gtgtccctaa agttcaagtt
1141 actccagcgc cagaggcatg taaacaaata tcagtttatt acaatggaag tttagcatct
1201 tcagcttcta atataacatt acattggggt tgtaatggat ttacgtcgcc tcaggatata
1261 aatatggtaa aacaggcgga tggaagatgg cttgcaaata taactttacc ttctggctgt
1321 tataatgtta atatggcatt taaagaccag tcaggtacat gggataataa taacagtaat
1381 aactataatt tttcttcaac aaataattaa gtgaaaaatc aatggcttgt acaatctagt
1441 acaggccatt taatttaact ttctctttag tttctttaaa gcactgtttt ttcgtctaag
1501 acatgttcta taatttatgc ctgatttttt agaatactca atagaagagt atcctaaaaa
1561 gtaaatatgg tctataatat cttgttcttt cttgtctaaa ctggttaagg catttttgat
1621 agataagttc ttaaaatgat tcgttttatc tataagaata tcttgaaaag tgcagttttg
1681 gtcagttact tttgcgttaa ggcttaattc agactggaat tttgcttgat ttcttatttt
1741 ataatatatg ttatttttaa tggaacttat ggtgtaagca gtgaagttat atttttttat
1801 gtcacatttt ttgattgata acagaattga aaagttacat tcctcgtata tatcgctttt
1861 gtcgaagttt cttatatata tgtttttagt gaaacttttt ataaatgggc caaaatagtt
1921 aaatatttta tttaaagagg cattatcacc agagcgtgat ttaagaacca aaatatttag
1981 gttttcgtta gataaaggct tcattaaaaa acctccaatt tatttatttt ttgtattgga
2041 attgtttata gtgtttgtga gattatttat ggagcttgta agggcttcca ttttaccttc
2101 tattctaact aaaaggtata ttgatacagc tatgggaaag ccaacattgc ctataagaga
2161 cactatatcg cctgaattca taaaaacacc tccacataag tttatataaa tctattatgc
2221 agaattttta ggaagtgtac ttttattttc gaaagctagt agaaaaaatt aatatatggt
2281 gtaaaaattt acttaaaaaa acaatatgtg ttataatgta aatataaata aataggacta
2341 gaggcgattt ataatgtgaa gataaagtat gttagaaaag ctaaacatta ttaaatttag
2401 gaaggtgact tttatgttaa aggatgaagt aattaaacaa attagcacgc cattaacttc
2461 gcctgcattt cctagaggac cctataaatt tcataatcgt gagtatttta acattgtata
2521 tcgtacagat atggatgcac ttcgtaaagt tgtgccagag cctttagaaa ttgatgagcc
2581 cttagtcagg tttgaaatta tggcaatgca tgatacgagt ggacttggtt gttatacaga
2641 aagcggacag gctattcccg taagctttaa tggagttaag ggagattatc ttcatatgat
2701 gtatttagat aatgagcctg caattgcagt aggaagggaa ttaagtgcat atcctaaaaa
2761 gctcgggtat ccaaagcttt ttgtggattc agatacttta gtaggaactt tagactatgg
2821 aaaacttaga gttgcgacag ctacaatggg gtacaaacat aaagccttag atgctaatga
2881 agcaaaggat caaatttgtc gccctaatta tatgttgaaa ataataccca attatgatgg
2941 aagccctaga atatgtgagc ttataaatgc gaaaatcaca gatgttaccg tacatgaagc
3001 ttggacagga ccaactcgac tgcagttatt tgatcacgct atggcgccac ttaatgattt
3061 gccagtaaaa gagattgttt ctagctctca cattcttgca gatataatat tgcctagagc
3121 tgaagttata tatgattatc ttaagtaata aaaataagag ttaccttaaa tggtaactct
3181 tattttttta atattgtttc atagtatttc tttctaaaca gccatgggtc taagttcatt
3241 ggatatgagt aaatctgcag cagttaaaga ccttatttca tcaatggttg tgtttttatt
3301 aatttcagtg agaagtaaac catcattaat aacctcaatt actccaagtt ctgttacaat
3361 tagatttgct tgagactttg ccgtgagggg aagtgtacat ttttttaaaa ttttaggttg
3421 acctttattt gtatgtctca ttgcaattat tactttctta gctccattta ctaaatccat
3481 agctccaccc ataccagaga gcatttttcc aggaacaatc caattggcta tattaccctt
3541 ttcatctacc tggagagccc ctaaaacagt aacatctacg tgaccaccac ggattagtga
3601 aaacgaaact gagctatcga aaaatgtgcc gtcaggaagt actgttgtat agtctcctcc
3661 tgcatttact acatctttat ctgcctcatt tattttagga ctagcgccca ttccaactat
3721 tccgttttct gattggaaag taattttgaa attttttggt atataatctg caaccatggt
3781 aggaagacct acacctaagt ttacaagttg accatttttt aattctcttg caactctttt
3841 ggctattatt tctttcgcta ggtttttatc attaatcatt ttatgcaggc tcctttacta
3901 tataatttat aagaactccg ggggtcattg ctttttcctt ttctagtttt tcacagctaa
3961 ctaaattttc agcttcaact attacggttt tagctgccat tgccatatag ggattaaagt
4021 ttttagtagt acctttatag aaggtgtttc cggt
//