GenBank-Updates@genbank.bio.net (11/02/90)
LOCUS VACRPO132 4914 bp ds-DNA VRL 02-NOV-1990
DEFINITION Vaccinia virus DNA-dependent RNA polymerase-subunit gene, complete
cds.
ACCESSION M37415
KEYWORDS DNA-dependent RNA polymerase-subunit rpo132.
SOURCE Vaccinia virus (strain WR) DNA.
ORGANISM Vaccinia virus
Viridae; ds-DNA enveloped viruses; Poxviridae; Orthopoxvirus.
REFERENCE 1 (bases 1 to 4914)
AUTHORS Amegadzie,B.Y., Holmes,M.H., Cole,N.B., Jones,E.V., Earl,P.L. and
Moss,B.
TITLE Identification, sequence, and expression of the gene encoding the
second-largest subunit of the vaccinia virus DNA-dependent RNA
polymerase
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by B.Y.Amegadzie, 03-AUG-1990.
Author address: B.Y.Amegadzie NIAID, Lab of Viral Diseases, 9000
Rockville Pike, Bethesda, MD 20892 USA
FEATURES from to/span description
pept 455 3949 RNA polymerase-subunit rpo132
pept 4583 3954 (c) ORF
BASE COUNT 1598 a 863 c 940 g 1513 t
ORIGIN
1 gtatacacat gattgacaaa ttattctatg tctattataa tttatttctg ttgttcgaag
61 atatcatcca aaacgagtac tttaaagaag tagctaatgt tgtaaaccac gtacttacgg
121 ctacggcatt agatgagaaa ttattcctaa ttaagaaaat ggctgaacac gatgtttatg
181 gagttagcaa tttcaaaata gggatgttta acctgacatt tattaagtcg ttggatcata
241 ccgttttccc ctctctgtta gatgaggata gcaaaataaa gttttttaag gggaaaaagc
301 tcaatattgt agcattacga tctctggagg attgtataaa ttacgtgact aaatccgaga
361 atatgataga aatgatgaag gaaagatcga ctattttaaa tagcatagat atagaaacgg
421 aatcggtaga tcgtctaaaa gaattgcttc taaaatgaaa aaaaacactg attcagaaat
481 ggatcaacga ctagggtata agtttttggt gcctgatcct aaagccggag ttttttatag
541 accgttacat ttccaatatg tatcgtattc taattttata ttgcatcgat tgcatgaaat
601 cttgaccgtc aagcggccac tcttatcgtt taagaataat acagaacgaa ttatgataga
661 aattagcaat gttaaagtga ctcctccaga ttactcacct ataatcgcga gtattaaagg
721 taagagttat gatgcattag ccacgttcac tgtaaatatc tttaaagagg taatgaccaa
781 agagggtata tccatcacta aaataagtag ttatgaggga aaagattctc atttgataaa
841 aattccgcta ctaataggat acgggaataa aaatccactt gatacagcca agtatcttgt
901 tcctaatgtc ataggtggag tctttatcaa taaacaatct gtcgaaaaag taggaattaa
961 tctagtagaa aagattacaa catggccaaa atttagggtt gttaagccaa actcattcac
1021 tttctcgttt tcctccgtat cccctcctaa tgtattaccg acaagatatc gccattacaa
1081 gatatctctg gatatatcac aattggaagc gttgaatata tcatcgacaa agacatttat
1141 aacggtcaat attgttttgc tgtctcaata tttatctaga gtgagtctag aattcattag
1201 acgtagttta tcatacgata tgcctccaga agttgtctat ctagtaaacg cgataataga
1261 tagtgctaaa cgaattactg aatctattac tgactttaat attgatacat acattaatga
1321 cctggtggaa gctgaacaca ttaaacaaaa atctcagtta acgatcaacg agttcaaata
1381 tgaaatgctg cataactttt tacctcatat gaactataca cccgatcaac taaagggatt
1441 ttatatgata tctttactaa gaaagtttct ctactgtatc taccacactt ctagatatcc
1501 agatagagat tcgatggttt gtcatcgcat cctaacgtac ggcaaatatt ttgagacgtt
1561 ggcacatgat gaattagaga attacatagg caacatccga aacgatatca tgaacaatca
1621 caagaacaga ggcacttacg cggtaaacat tcatgtacta acaactcccg gacttaatca
1681 cgcgttttct agcttattga gtggaaagtt caaaaagtca gacggtagtt atcgaacaca
1741 tcctcactat tcatggatgc agaatatttc tattcctagg agtgttggat tttatccgga
1801 tcaagtaaag atttcaaaga tgttttctgt cagaaaatac catccaagtc aatatcttta
1861 cttttgttca tcggacgttc cggaaagagg tcctcaggta ggtttagtat ctcaattgtc
1921 tgtcttgagt tccattacaa atatactaac gtctgagtat ttggatttgg aaaagaaaat
1981 ttgtgagtat atcagatcat attataaaga tgatataagt tactttgaaa caggatttcc
2041 aatcactata gaaaatgctc tagtcgcatc tcttaatcca aatatgatat gtgattttgt
2101 aactgacttt agacgtagaa aacggatggg attcttcggt aacttggagg taggtattac
2161 tttagttagg gatcacatga atgaaattcg cattaatatt ggagcgggaa gattagtcag
2221 accattcttg gttgtggata acggagagct catgatggat gtgtgtccgg agttagaaag
2281 cagattagac gacatgacat tctctgacat tcagaaagag tttccgcatg tcatcgaaat
2341 ggtagatata gaacaattta cttttagtaa cgtatgtgaa tcggttcaaa aatttagaat
2401 gatgtcaaag gatgaaagaa agcaatacga tttatgtgac tttcctgccg aatttagaga
2461 tggatatgtg gcatcttcat tagtgggaat caatcacaat tctggaccca gagctattct
2521 tggatgtgct caagctaaac aagctatctc ttgtctgagt tcggatatac gaaataaaat
2581 agacaatgga attcatttga tgtatccaga gaggccaatc gtgattagta aggctttaga
2641 aacttcaaag attgcggcta attgcttcgg ccaacatgtt actatagcat taatgtcgta
2701 caaaggtatc aatcaagagg atggaattat catcaaaaaa caatttattc agagaggcgg
2761 tctcgatata gttaccgcaa agaaacatca agtagaaatt ccgttggaaa actttaataa
2821 caaagaaaga gataggtcta acgcctattc aaaattagaa agtaatggat tagttagact
2881 gaatgctttc ttggaatccg gagacgctat ggcacgaaat atctcatcaa gaactcttga
2941 agatgatttt gctagagata atcagattag cttcgatgtt tccgagaaat ataccgatat
3001 gtacaaatct cgcgttgaac gagtacaagt agaacttact gacaaagtta aggtacgagt
3061 attaaccatg aaagaaagaa gacccattct aggagataaa tttaccacta gaacgagtca
3121 aaagggaaca gtcgcgtatg tcgcggatga aacggaactt ccatacgacg aaaatggtat
3181 cacaccagat gtcattatta attctacatc catcttctct agaaaaacta tatctatgtt
3241 gatagaggtt attttaacag ccgcatattc tgctaagccg tacaacaata agggagaaaa
3301 ccgacctgtc tgttttccta gtagtaacga aacatccatc gatacatata tgcaattcgc
3361 taaacaatgt tatgagcatt caaatccgaa attgtccgat gaagaattat cggataaaat
3421 cttttgtgaa aagattctct atgatcctga aacggataag ccttatgcat ccaaagtatt
3481 ttttggacca atttattact tgcgtctgag gcatttaact caggacaagg caaccgttag
3541 atgtagaggt aaaaagacga agctcattag acaggcgaat gagggacgaa aacgtggagg
3601 aggtatcaag ttcggagaaa tggagagaga ctgtttaata gcgcatggcg cagccaatac
3661 tattacagaa gttttaaaag actcagaaga ggattatcaa gatgtgtatg tttgtgaaaa
3721 ttgtggagac atagcagcac aaatcaaggg tattaataca tgtcttagat gttcaaaact
3781 taatctctct cctctcttaa caaaaattga taccacgcac gtatctaaag tatttcttac
3841 tcaaatgaac gccagaggcg taaaagtcaa attagatttc gaacgaaggc ctccttcgtt
3901 ttataaacca ttagataaag ttgatctcaa gccgtctttt ctggtgtaat attctagttt
3961 ggtagtagat acatatcaat atcatcaaat tcgagatccg aattataaaa tgggcgtgga
4021 ttgttaacta tagaatcgga cgtctgatat tcgaaaatct gtggagtttt aggttttggt
4081 ggaggtgtaa ctgctacttg ggatactgaa gtctgatatt cagaaagctg ggggatgttc
4141 tggttcgaca tccacgatgg tgtcacatca ctaatcggtt cggtaacgtc tgtggatgga
4201 ggtgctactt ctacagaacc tgtagcctca gttgtcaacg gagatacatt tttaatgcga
4261 ggaaatgtat aatttggtaa tggtttctca tgtggatctg aagaagaggt aagatatcta
4321 ctagaaagat accgatcacg ttctagttct cttttgtaga acttaacttt ttctttctca
4381 gcatctagtt gatattccaa cctcttcacg ttactacgtt cagattccaa ttcacgttcg
4441 catgggttac ctccgcagtt tttacgagcg atttcacgtt cagccttcat gcgtctctcc
4501 ttctctctat cgagtttatc agagcagtct ttctgaaggc gatcgaactc cataaatttc
4561 tccaacgctt tgattgtttc catagatttc cgaagttcag cttttaggac tgtgattctt
4621 tttctttcga attcacagct ggatgtgcaa ccgtttccat taccgccatc tctaagtttc
4681 ttttctagat cggcaacatt tcatccccat gccttttaca ttcctcgagt ctactgtcgt
4741 cgaaatatcg ttccagctcc ttttcgacat caataacttt agcacgttgt ctctcaagct
4801 ctcttttgta gttatctgat tccctggcac gtttaagatc ttcatgcaat tgagtcagct
4861 cttaacacaa tctcttgctt cttcgtcata gtacttacaa tcactatggg atcc
//GenBank-Updates@genbank.bio.net (01/25/91)
LOCUS VACRPO132 4914 bp ds-DNA VRL 25-JAN-1991
DEFINITION Vaccinia virus DNA-dependent RNA polymerase-subunit gene, complete
cds.
ACCESSION M37415
KEYWORDS DNA-dependent RNA polymerase-subunit rpo132.
SOURCE Vaccinia virus (strain WR) DNA.
ORGANISM Vaccinia virus
Viridae; ds-DNA enveloped viruses; Poxvirinae; Orthopoxvirus.
REFERENCE 1 (bases 1 to 4914)
AUTHORS Amegadzie,B.Y., Holmes,M.H., Cole,N.B., Jones,E.V., Earl,P.L. and
Moss,B.
TITLE Identification, sequence, and expression of the gene encoding the
second-largest subunit of the vaccinia virus DNA-dependent RNA
polymerase
JOURNAL Virology 180, 88-98 (1991)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)
NIAID, Lab of Viral Diseases, 9000 RockvillePike, Bethesda, M]
kindly submitted
by B.Y.Amegadzie, 03-AUG-1990.
FEATURES Location/Qualifiers
CDS 455..3949
/codon_start=455
/product="RNA polymerase-subunit rpo132"
CDS complement(3949..4583)
/codon_start=3949
BASE COUNT 1598 a 863 c 940 g 1513 t
ORIGIN
1 gtatacacat gattgacaaa ttattctatg tctattataa tttatttctg ttgttcgaag
61 atatcatcca aaacgagtac tttaaagaag tagctaatgt tgtaaaccac gtacttacgg
121 ctacggcatt agatgagaaa ttattcctaa ttaagaaaat ggctgaacac gatgtttatg
181 gagttagcaa tttcaaaata gggatgttta acctgacatt tattaagtcg ttggatcata
241 ccgttttccc ctctctgtta gatgaggata gcaaaataaa gttttttaag gggaaaaagc
301 tcaatattgt agcattacga tctctggagg attgtataaa ttacgtgact aaatccgaga
361 atatgataga aatgatgaag gaaagatcga ctattttaaa tagcatagat atagaaacgg
421 aatcggtaga tcgtctaaaa gaattgcttc taaaatgaaa aaaaacactg attcagaaat
481 ggatcaacga ctagggtata agtttttggt gcctgatcct aaagccggag ttttttatag
541 accgttacat ttccaatatg tatcgtattc taattttata ttgcatcgat tgcatgaaat
601 cttgaccgtc aagcggccac tcttatcgtt taagaataat acagaacgaa ttatgataga
661 aattagcaat gttaaagtga ctcctccaga ttactcacct ataatcgcga gtattaaagg
721 taagagttat gatgcattag ccacgttcac tgtaaatatc tttaaagagg taatgaccaa
781 agagggtata tccatcacta aaataagtag ttatgaggga aaagattctc atttgataaa
841 aattccgcta ctaataggat acgggaataa aaatccactt gatacagcca agtatcttgt
901 tcctaatgtc ataggtggag tctttatcaa taaacaatct gtcgaaaaag taggaattaa
961 tctagtagaa aagattacaa catggccaaa atttagggtt gttaagccaa actcattcac
1021 tttctcgttt tcctccgtat cccctcctaa tgtattaccg acaagatatc gccattacaa
1081 gatatctctg gatatatcac aattggaagc gttgaatata tcatcgacaa agacatttat
1141 aacggtcaat attgttttgc tgtctcaata tttatctaga gtgagtctag aattcattag
1201 acgtagttta tcatacgata tgcctccaga agttgtctat ctagtaaacg cgataataga
1261 tagtgctaaa cgaattactg aatctattac tgactttaat attgatacat acattaatga
1321 cctggtggaa gctgaacaca ttaaacaaaa atctcagtta acgatcaacg agttcaaata
1381 tgaaatgctg cataactttt tacctcatat gaactataca cccgatcaac taaagggatt
1441 ttatatgata tctttactaa gaaagtttct ctactgtatc taccacactt ctagatatcc
1501 agatagagat tcgatggttt gtcatcgcat cctaacgtac ggcaaatatt ttgagacgtt
1561 ggcacatgat gaattagaga attacatagg caacatccga aacgatatca tgaacaatca
1621 caagaacaga ggcacttacg cggtaaacat tcatgtacta acaactcccg gacttaatca
1681 cgcgttttct agcttattga gtggaaagtt caaaaagtca gacggtagtt atcgaacaca
1741 tcctcactat tcatggatgc agaatatttc tattcctagg agtgttggat tttatccgga
1801 tcaagtaaag atttcaaaga tgttttctgt cagaaaatac catccaagtc aatatcttta
1861 cttttgttca tcggacgttc cggaaagagg tcctcaggta ggtttagtat ctcaattgtc
1921 tgtcttgagt tccattacaa atatactaac gtctgagtat ttggatttgg aaaagaaaat
1981 ttgtgagtat atcagatcat attataaaga tgatataagt tactttgaaa caggatttcc
2041 aatcactata gaaaatgctc tagtcgcatc tcttaatcca aatatgatat gtgattttgt
2101 aactgacttt agacgtagaa aacggatggg attcttcggt aacttggagg taggtattac
2161 tttagttagg gatcacatga atgaaattcg cattaatatt ggagcgggaa gattagtcag
2221 accattcttg gttgtggata acggagagct catgatggat gtgtgtccgg agttagaaag
2281 cagattagac gacatgacat tctctgacat tcagaaagag tttccgcatg tcatcgaaat
2341 ggtagatata gaacaattta cttttagtaa cgtatgtgaa tcggttcaaa aatttagaat
2401 gatgtcaaag gatgaaagaa agcaatacga tttatgtgac tttcctgccg aatttagaga
2461 tggatatgtg gcatcttcat tagtgggaat caatcacaat tctggaccca gagctattct
2521 tggatgtgct caagctaaac aagctatctc ttgtctgagt tcggatatac gaaataaaat
2581 agacaatgga attcatttga tgtatccaga gaggccaatc gtgattagta aggctttaga
2641 aacttcaaag attgcggcta attgcttcgg ccaacatgtt actatagcat taatgtcgta
2701 caaaggtatc aatcaagagg atggaattat catcaaaaaa caatttattc agagaggcgg
2761 tctcgatata gttaccgcaa agaaacatca agtagaaatt ccgttggaaa actttaataa
2821 caaagaaaga gataggtcta acgcctattc aaaattagaa agtaatggat tagttagact
2881 gaatgctttc ttggaatccg gagacgctat ggcacgaaat atctcatcaa gaactcttga
2941 agatgatttt gctagagata atcagattag cttcgatgtt tccgagaaat ataccgatat
3001 gtacaaatct cgcgttgaac gagtacaagt agaacttact gacaaagtta aggtacgagt
3061 attaaccatg aaagaaagaa gacccattct aggagataaa tttaccacta gaacgagtca
3121 aaagggaaca gtcgcgtatg tcgcggatga aacggaactt ccatacgacg aaaatggtat
3181 cacaccagat gtcattatta attctacatc catcttctct agaaaaacta tatctatgtt
3241 gatagaggtt attttaacag ccgcatattc tgctaagccg tacaacaata agggagaaaa
3301 ccgacctgtc tgttttccta gtagtaacga aacatccatc gatacatata tgcaattcgc
3361 taaacaatgt tatgagcatt caaatccgaa attgtccgat gaagaattat cggataaaat
3421 cttttgtgaa aagattctct atgatcctga aacggataag ccttatgcat ccaaagtatt
3481 ttttggacca atttattact tgcgtctgag gcatttaact caggacaagg caaccgttag
3541 atgtagaggt aaaaagacga agctcattag acaggcgaat gagggacgaa aacgtggagg
3601 aggtatcaag ttcggagaaa tggagagaga ctgtttaata gcgcatggcg cagccaatac
3661 tattacagaa gttttaaaag actcagaaga ggattatcaa gatgtgtatg tttgtgaaaa
3721 ttgtggagac atagcagcac aaatcaaggg tattaataca tgtcttagat gttcaaaact
3781 taatctctct cctctcttaa caaaaattga taccacgcac gtatctaaag tatttcttac
3841 tcaaatgaac gccagaggcg taaaagtcaa attagatttc gaacgaaggc ctccttcgtt
3901 ttataaacca ttagataaag ttgatctcaa gccgtctttt ctggtgtaat attctagttt
3961 ggtagtagat acatatcaat atcatcaaat tcgagatccg aattataaaa tgggcgtgga
4021 ttgttaacta tagaatcgga cgtctgatat tcgaaaatct gtggagtttt aggttttggt
4081 ggaggtgtaa ctgctacttg ggatactgaa gtctgatatt cagaaagctg ggggatgttc
4141 tggttcgaca tccacgatgg tgtcacatca ctaatcggtt cggtaacgtc tgtggatgga
4201 ggtgctactt ctacagaacc tgtagcctca gttgtcaacg gagatacatt tttaatgcga
4261 ggaaatgtat aatttggtaa tggtttctca tgtggatctg aagaagaggt aagatatcta
4321 ctagaaagat accgatcacg ttctagttct cttttgtaga acttaacttt ttctttctca
4381 gcatctagtt gatattccaa cctcttcacg ttactacgtt cagattccaa ttcacgttcg
4441 catgggttac ctccgcagtt tttacgagcg atttcacgtt cagccttcat gcgtctctcc
4501 ttctctctat cgagtttatc agagcagtct ttctgaaggc gatcgaactc cataaatttc
4561 tccaacgctt tgattgtttc catagatttc cgaagttcag cttttaggac tgtgattctt
4621 tttctttcga attcacagct ggatgtgcaa ccgtttccat taccgccatc tctaagtttc
4681 ttttctagat cggcaacatt tcatccccat gccttttaca ttcctcgagt ctactgtcgt
4741 cgaaatatcg ttccagctcc ttttcgacat caataacttt agcacgttgt ctctcaagct
4801 ctcttttgta gttatctgat tccctggcac gtttaagatc ttcatgcaat tgagtcagct
4861 cttaacacaa tctcttgctt cttcgtcata gtacttacaa tcactatggg atcc
//