GenBank-Updates@genbank.bio.net (01/25/91)
LOCUS ECHOUT 2316 bp ds-DNA BCT 25-JAN-1991
DEFINITION Erwinia chrysanthemi peptic enzymes H,I,J, and K genes, 3' end,
complete, 3' end, and partial cds.
ACCESSION M37886
KEYWORDS pectic enzyme; secretion protein.
SOURCE Erwinia chrysanthemi (strain EC16) DNA.
ORGANISM Erwinia chrysanthemi
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2316)
AUTHORS He,S.Y., Lindeberg,M., Chatterjee,A. and Collmer,A.
TITLE Cloned out genes from Erwinia chrysanthemi EC16 allows Escherichia
coli to specifically secrete the multiple pectic enzymes
JOURNAL Unpublished (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by S.Y.He, 21-AUG-1990.
Dept Plant Pathology
334 Plant Science Bldg
Cornell University
Ithaca, NY 14953
FEATURES Location/Qualifiers
CDS <1..289
/gene="outH"
/codon_start=2
/product="peptic enzyme H"
CDS 293..670
/gene="outI"
/codon_start=293
/product="peptic enzyme I"
CDS 667..1287
/gene="outJ"
/codon_start=667
/partial
/product="peptic enzyme J"
CDS 1401..>2316
/gene="outK"
/codon_start=1402
/product="peptic enzyme K"
BASE COUNT 498 a 569 c 740 g 509 t
ORIGIN
1 gccggaagaa caacagcgtc tgcgctatgt ctggataccg tggcaaccgt accgcatgtc
61 ggttcccagc gagttgccgg acagctttca catcgagctg tctacacaag tcggggccgc
121 gggagacggc accggttttt ccccaggcaa cggcgatcct catgttctga ttctgccggg
181 cggcgaagtg acgccatttc gtctgacctt gcgtaatggc aacgattccg cctggttaca
241 ggtggatacc aacggtcagg tgcacacgtc gccggaagcg gagcagtaag ggatgaaaca
301 acagggaatg acgttgcttg aggtgatggt ggcactggtc atctttgcgc tggcgggttt
361 gacggtgctg aaaacgacgg ctcagcaggc gaatgggttg gggcgcctgg aagagaagac
421 cttcgcactc tggattgcag aaaatcaaca ggcggcgatg cggctggaaa aacagtggcc
481 gcagagccaa tgggtcgatg gtgaagtgaa tttcgccggt tcactgtggt tttggcgtat
541 tcagggcatc gccacggcgg atacgcgggt gcgggcaatt gatgttgaag ttcgtcatga
601 tcgcgacagc cgggcggcgg atgccgtctt gcgcagttat ctggtccagc ccggagaacc
661 tgcgcagtga agcagcctga acgcggtttt accctgctgg aagtgatgct ggcgctggcg
721 attttcgctg cgttgagtct ggctgcctcg caggtgatga acggtgtgat gcgcaatgac
781 gaggcgtctg cgcgtaaaga ggcgcgtctg gccgagttgc aacgcggttt ttccttgatg
841 gagcgggatt tttcacaaat cgttccgcgc cgcagccaag ggtatgagct ggggttttac
901 gccgagcgtt atcaattaag cagtgccgat tgggccgtca gttttattcg caacggctgg
961 ctcaaccctc tggggattat gccgcgttct gaattacagc gggtgggata ccggctacgc
1021 ggcgatacgc tggagcggct gttttacgat tcctcggaac cgctgtcgac gcaggaaccg
1081 gcggtacgcc cggtgttaac cggcgtgacg gggtttgttc tgcgcttttt cggtaaggac
1141 ggctggcagg agcgttggga tgacccggcc aatctgccgc aaggtatcgc tatcgttgtt
1201 accttacgcg attacggcga gattacccgt atattcctga tcacgccgcg ctacggagcc
1261 gcaacccaaa ggcggtccga acgataataa cagcgacgaa aataaggacc aacagaatca
1321 ggagcagcaa ccacaggaaa ataacgattc gtccggcgat cagagtcagg cctcgccgga
1381 tgataacggc ggaggtgact cgtgagtcgt cggcaacggg gtgtcgcttt gttgatcgtg
1441 atgctgatgc tgtcgttgat ggtcacgatt gcggcgtcta ttaccgagcg cagcggcaaa
1501 gcgtggcagc gtaccagcaa tctgttgaat cgtacccagg cgcgttggta tgcattaggc
1561 tttatcggtc agccctggtc aaaagtggat caccagatga tggcggacgg cactgaaatt
1621 cgtgctcagg cgctggacgg gcaggcatgt ctcaatctga acgcgttgag cccggctaga
1681 aacgtgacgc ccaataacgc atccggtaat aacacatccg gtaataacaa tgcggctaac
1741 ggcagtagcg gcaacggtaa ttcgcctcag ccgcccaaag tcggcacttc tgagcaagtg
1801 ccttatgcgg cacaggtttt tcggcagtta atgatcgtac tgggggaaga ccctaagcag
1861 gcggagcgta taaccgacgc cttacgggat tggctcgacg aagatagcga accgttgatg
1921 aatggggcgg aggatgacag ctatgtcaat tttcatcccg gtaatcagcg tatgacggac
1981 gttacggaat tacgggcggt catgggcatg gatgcggcgt tgtaccgccg gttattgcct
2041 tacgtttgcg tgttgccggt ggataaactg gcgattaacg tcaacaccct gatgccgggc
2101 agcgcgccgc tattatcggc gttatttatg ggcgatatca gtctggatat ggccgagcgt
2161 attttgcaac aacgaccgcc gcagggatgg cgcaatctta atgattttat gggaatgtcg
2221 cgctgccgga aagcggcaaa aatggcgctc gtcaggtgtt ggttatcaag agcgactggt
2281 tcttcgccga cattcagatt cgggtggacg acagtt
//