GenBank-Updates@genbank.bio.net (01/29/91)
LOCUS BSUGLPKD 4259 bp ds-DNA BCT 29-JAN-1991
DEFINITION B.subtilis glycerol kinase (glpK) and glycerol-3-phosphate
dehydrogenase (glpD) genes, complete cds.
ACCESSION M34393
KEYWORDS glycerol kinase; glycerol-3- phosphate dehydrogenase.
SOURCE B.subtilis (starin W168) DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4259)
AUTHORS Holmberg,C., Beijer,L., Rutberg,B. and Rutberg,L.
TITLE Glycerol catabolism in Bacillus subtilis: Nucleotide sequence of
the genes encoding glycerol kinase (glpK) and glycerol-3-phosphate
dehydrogenase (glpD)
JOURNAL J. Gen. Microbiol. 136, 2367-2375 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)
University of Lund, Dept. of Microbiology,Solvegatan 21, S-22]
kindly submitted
by C.M.E.Holmberg, 22-MAY-1990.
FEATURES Location/Qualifiers
CDS <1..679
/note="ORF 1 (AA at 2)"
misc_binding 682..690
/note="ribosome binding site"
CDS 698..2188
/note="glycerol kinase (glpK) (EC 2.7.1.30)"
misc_signal 2243..2303
/note="transcription termination signal"
misc_binding 2315..2321
/note="ribosome binding site"
CDS 2329..3996
/note="glycerol-3-phosphate dehydrogenase (glpD) (EC
1.1.99.5)"
misc_signal 4000..4033
/note="transcription termination signal"
misc_signal 4067..4072
/note="-35 region"
misc_signal 4090..4095
/note="-10 region"
misc_binding 4116..4121
/note="ribosome binding site"
CDS 4128..>4259
/note="ORF 4"
BASE COUNT 1269 a 853 c 1092 g 1045 t
ORIGIN
1 ccatggcgga tacgcggttg gcggcatcag cggtgcccat ttgaatccgg cgctaacgat
61 agcgcttgca tttgtaggag attttccttg gaaagaggtt ccggtttata ttgcggcgca
121 aatgatcgga gcgatcatcg gtgcggtgat tatttatctg cattacctcc cgcactggaa
181 gtcaacggat gatcccgctg ccaagctggg tgttttctca acagggccca gcattccgca
241 tacatttgca aacgttttaa gcgaagtgat tgggacattt gtccttgtac ttggaatctt
301 ggccataggt gcaaatcaat ttacagaagg acttaatcct ttaatcgtcg gtttcctcat
361 tgtagcaatc ggtatttctc tgggaggcac caccggctat gctatcaatc ctgcacgtga
421 cttaggtccg cggatcgccc acgcttttct tccgattccg gggaagggct catcaaactg
481 gaaatacgca tgggttccgg tagtcggccc gattttaggc ggatcattcg gcggtgtatt
541 ttacaacgct gcatttaaag ggcacatcac aagcagcttc tggattgtaa gcgttatatt
601 ggttgtggta ttgttaggac tctatgttta tacgaaatca cattctgcta aaacattatc
661 aaattctaaa tatatttaat caaaggggag acatcttatg gaaacgtaca ttttatcctt
721 agatcagggg acgacaagtt caagagcgat tctgtttaat aaagaaggca aaattgtcca
781 ctctgctcaa aaggaattta cacaatactt cccgcatcct ggctgggttg agcataatgc
841 caatgaaatt tggggctctg tcctcgcggt tatcgcctca gtcatctctg aatcaggaat
901 cagcgcttct caaattgccg gcatcggcat cacgaaccag cgcgagacga cggttgtgtg
961 ggataaagat acaggaagtc ctgtctataa tgcaatcgtt tggcagtcca gacagacgtc
1021 cggcatttgt gaggaacttc gtgaaaaagg atataatgat aaattcagag aaaaaacagg
1081 gcttttaatc gatccttact tctccggcac gaaggtgaag tggattttag acaatgtgga
1141 aggcgcaaga gaaaaagcgg aaaaaggcga gctgctgttt ggaacgattg atacgtggct
1201 catttggaaa atgtcaggcg gaaaagcgca tgtgaccgat tactccaatg cctcaagaac
1261 actgatgttt aatatttacg atttaaaatg ggacgatcaa ctgctcgaca ttctaggcgt
1321 accgaaatcc atgctccctg aagtgaagcc gtcctctcat gtgtatgcgg agactgttga
1381 ttatcacttc ttcggaaaaa atatcccgat tgctggagcg gcaggcgacc agcagtccgc
1441 attgttcggc caggcatgct ttgaagaagg catggggaaa aacacttacg gcacaggatg
1501 tttcatgctg atgaataccg gggaaaaagc aattaagtcc gaacatgggc ttttgacaac
1561 aatcgcttgg ggcattgacg gaaaagtgaa ctatgcgtta gaagggagca tttttgtcgc
1621 aggctctgcc atccagtggc ttagagacgg tttgagaatg ttccaggatt catcgctaag
1681 cgaatcttat gcagaaaaag tggattcaac tgacggcgtg tatgttgttc cagcatttgt
1741 cggactggga acgccttact gggacagcga tgtgcgcggt tcggttttcg gcctgacaag
1801 agggacaaca aaagagcact ttatccgtgc gacactggag tcattggctt atcagaccaa
1861 agatgtgctt gacgcaatgg aagcagattc aaacatttca ttaaagacgc tccgtgtaga
1921 cggaggagct gtaaaaaaca atttcctaat gcagttccaa ggagacctgt tgaatgttcc
1981 tgtggagcgc ccggaaatta atgaaacgac tgcacttggc gcggcttatt tggcgggtat
2041 cgctgtggga ttctggaagg accgttctga aatcgcgaac cagtggaatc tggataaacg
2101 gtttgagcct gaattggaag aagaaaaacg aaatgagctg tataaaggct ggcaaaaagc
2161 cgtgaaagca gctatggctt ttaaataaag taatactatg gtataatggt tacaagttaa
2221 taagaacggt cctgagatga ggagagacca cagcaccaaa gtgtaagcat gcactttggc
2281 tgttgtggtc tctttttcta tttaccgtga caacaaggag gaaacgtaat gatgaatcat
2341 caattttcaa gtcttgaaag agatcgcatg ctgacagaca tgacgaaaaa aacatatgac
2401 ctatttatta tcggaggagg aattacagga gccggaacag ctcttgacgc ggcatcaagg
2461 ggaatgaagg tcgcactaag cgaaatgcag gactttgcgg cgggaacatc aagccgatca
2521 acgaagctgg tacatggcgg cttgcgctat ttaaaacaat ttgaagtgaa aatggtcgct
2581 gaggtaggaa aagagcgggc gattgtgtat gaaaacggcc cgcatgtcac tacaccggaa
2641 tggatgctgc ttccgtttca taaaggcggc acattcggtt catttacaac atctattggg
2701 ttaagggttt atgacttcct tgcaggtgtg aaaaagtcag aacgaagaag catgctttca
2761 gcaaaagaaa cgctgcaaaa agagcctttg gtgaaaaaag acggcttaaa aggcggcggg
2821 tactatgtgg aataccgcac tgacgatgcg agactgacca tcgaagtcat gaaggaagcg
2881 gttaaattcg gggcagagcc tgtgaattac tccaaagtga aggagcttct ttacgaaaaa
2941 ggcaaagccg tcggcgtatt aattgaagat gtgctgacaa agaaagaata taaagtgtat
3001 gcgaaaaaaa ttgtcaatgc tacaggccct tgggtcgatc agctcagaga aaaagaccat
3061 tcgaaaaacg gaaagcattt gcagcataca aaaggcattc accttgtatt tgaccagtct
3121 gtctttccgc tgaaacaggc tgtatatttc gatacacctg atggccggat ggtatttgcg
3181 attcctcgtg aaggcaaaac atacgtggga acaacagaca ctgtttacaa agaggcgctg
3241 gagcatccgc ggatgacaac ggaggatcgt gattatgtca tcaaatcaat caattacatg
3301 ttcccggaac tgaatatcac tgcgaatgac atcgaatcca gctgggcggg gctgcgtccg
3361 ctgattcatg aagaaggcaa agatccttct gaaatttcac ggaaagacga gatttggaca
3421 tctgactcag gcctgatcac cattgccggc ggaaaactga ctggatacag aaaaatggcg
3481 gacgacatcg ttgatcttgt ccgtgatcgc ttaaaagaag agggcgaaaa ggattttgga
3541 ccatgtaaaa cgaaaaacat gccaatctct ggcgggcacg tcggcggttc gaaaaatctt
3601 atgtccttcg ttaccgcgaa aacaaaagaa ggaattgcag ccggtttatc agaaaaagac
3661 gcaaaacagc ttgcgatcag atacggctct aacgtagatc gcgtctttga ccgggtagaa
3721 gcgctgaaag atgaggccgc gaaacgcaac attccggttc atattcttgc tgaggcagaa
3781 tacagtatag aagaagagat gactgcaacc cctgctgact tctttgtccg cagaacggga
3841 cgtttatttt ttgatatcaa ttgggtaaga acatataaag atgccgttat tgattttatg
3901 agcgagcgat tccaatggga tgagcaggcg aaaaacaaac atacagaaaa cctcaacaag
3961 cttttacacg atgcggtcgt accgcttgag caataaatca taacgggctg tctgcagccc
4021 gttatttctt tttacgtgcc gaaaggggga gatctcaggt tatcaagtga cagaggcacc
4081 cgcttcatat aaaataatgg cataagctga tacataggag gacgaatatg acttggagaa
4141 agagctatga acgctggaaa cagacagaac atttagatct ggaattaaaa gagcgcctta
4201 ttgaattaga gggagatgaa caggcccttg aggactgttt ctataaagac cttgaattc
//