GenBank-Updates@genbank.bio.net (01/29/91)
LOCUS TATPROCLOT 1501 bp ss-mRNA INV 29-JAN-1991
DEFINITION Tachypleus tridentatus proclotting enzyme mRNA, complete cds.
ACCESSION M58366
KEYWORDS proclotting enzyme.
SOURCE Tachypleus tridentatus hemocyte, cDNA to mRNA.
ORGANISM Tachypleus tridentatus
Eukaryota; Animalia; Metazoa; Arthropoda; Chelicerata; Merostomata;
Xiphosura; Xiphosurida; Limulidae.
REFERENCE 1 (bases 1 to 1501)
AUTHORS Muta,T., Hashimoto,R., Miyata,T., Nishimura,H., Toh,Y. and
Iwanaga,S.
TITLE Proclotting enzyme from horseshoe crab hemocytes: cDNA cloning,
disulfide locations, and subcellular localization
JOURNAL J. Biol. Chem. 265, 22426-22433 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
mat_peptide join(304..594,595..1341)
/evidence=EXPERIMENTAL
/product="proclotting enzyme"
sig_peptide 217..303
/codon_start=217
/product="proclotting enzyme"
CDS 217..1344
/codon_start=217
/product="proclotting enzyme"
mat_peptide 304..594
/note="light chain of larger mature peptide"
/evidence=EXPERIMENTAL
/codon_start=304
/product="proclotting enzyme"
mat_peptide 595..1341
/note="heavy chain of larger mature peptide"
/evidence=EXPERIMENTAL
/codon_start=595
/product="proclotting enzyme"
BASE COUNT 458 a 310 c 326 g 407 t
ORIGIN
1 gcaatacagg ctacaaacat ctcatcagac gcattacctg gttgtttaat tccgcgaaag
61 cttcacgaga acaagtcaaa cttagcttgt ggtcccgcga ctgactgcct ggaaagtagt
121 tcccaaaatt gcggctttat aaatcaacct aaacaaaacc gtcctgaaac tttaccgttc
181 cacatccacc gaggtagccg ggtcgtcctc agcagtatgt tggtgaataa cgtgttttca
241 ctactgtgtt tcccactctt gatgtctgtg gttagatgca gtactctcag cagacagcgt
301 agacagtttg ttttccctga cgaggaagaa ctttgctcaa accgatttac tgaagaagga
361 acatgcaaaa atgtcttgga ttgtagaata cttttacaaa aaaatgatta taatttactc
421 aaagaatcaa tatgcggctt tgaaggcata acacccaaag tttgttgtcc gaaatcaagc
481 catgtaattt caagtacaca ggcacctcca gaaaccacta cgactgaacg cccaccaaaa
541 cagataccac ccaatcttcc tgaagtgtgt ggaattcaca atactacaac taccaggatt
601 attggaggtc gggaagcacc tattggagcc tggccgtgga tgactgctgt ctacataaaa
661 caaggaggaa tcagaagtgt tcagtgtggt ggcgcacttg tcactaacag gcacgtgatt
721 acagcttcgc actgtgttgt aaacagtgca ggaacagatg tgatgccagc tgatgtattc
781 tcggttcgtc tgggtgaaca caatttatac agtaccgatg acgattcgaa tccaatagat
841 tttgcagtta cgtcggtgaa acatcacgaa cactttgtac tcgcgacgta tttgaatgac
901 atcgcaattc taacgttaaa tgacacagtt acgtttacag acagaattcg acccatttgt
961 ctaccttatc gtaagttgag atacgatgat ctagcaatga gaaaaccgtt tatcactgga
1021 tggggaacaa cagcatttaa cggcccatct agtgcagtgt tgagagaagt acagttacca
1081 atatgggaac acgaggcctg tagacaggcc tacgagaagg atttaaatat tacaaacgtg
1141 tatatgtgtg ctggctttgc agatggcggg aaggatgctt gccagggtga ttctggaggt
1201 ccaatgatgt tgcctgttaa aaccggagag ttttatctca ttggaattgt gtctttcgga
1261 aagaaatgcg cattgcctgg atttcctggg gtttacacaa aagtgacaga gtttttagat
1321 tggattgcag aacatatggt gtagactaaa gctgtgaatg actccatttc gaatatttaa
1381 cccatactcg cctgtagcaa aacgactgaa gaatcaagca ggggacaaaa ccaacttttt
1441 tttttttttt tcttcaatag ttctaccatg tattgaaaac caacataaga caatcaaata
1501 a
//