GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS TFEMERAC 3017 bp ds-DNA BCT 16-JAN-1991 DEFINITION T.ferrooxidans mer operon. ACCESSION D90110 M32353 KEYWORDS merA gene; merC gene; mercuric reductase; mercury resistance. SOURCE T.ferrooxidans (strain E-15) DNA; clones pTM31[4,5]. ORGANISM Thiobacillus ferrooxidans Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Colorless sulfur bacteria. REFERENCE 1 (bases 1379 to 3017) AUTHORS Inoue,C., Sugawara,K., Shiratori,T., Kusano,T. and Kitagawa,Y. TITLE Nucleotide sequence of the Thiobacillus ferrooxidans chromosomal gene encoding mercuric reductase JOURNAL Gene 84, 47-54 (1989) STANDARD simple staff_review REFERENCE 2 (bases 1 to 1378) AUTHORS Inoue,C., Sugawara,K. and Kusano,T. TITLE Thiobacillus ferrooxidans mer operon: Sequence analysis of the promoter and adjacent genes JOURNAL Gene 96, 115-120 (1990) STANDARD full staff_entry COMMENT These data kindly submitted in computer readable form by: Tomonobu Kusano Biotechnology Institute Akita Prefectural college of Agriculture Ohgata, Minami-Akitagun Akita 010-04 Japan Phone: 0185-45-2026 x403 Fax: 0185-45-2678 FEATURES Location/Qualifiers CDS 577..798 /note="URF-1" misc_signal 795..800 /note="-35 region" misc_signal 820..825 /note="-10 region" CDS 895..1326 /note="merC" CDS 1352..2989 /note="mercuric reductase (merA)" BASE COUNT 626 a 889 c 904 g 598 t ORIGIN 1 gtcgacaggt actccggtca gacgatatat tcttttttcc agcggtccca ggaaacgggt 61 ggcccagaat cgatgacctt caacaacacg atacatatag tggcccaacg gcaatgccag 121 aaaaactgac aatgccagaa ctgcaacaag gagaatgatt gcagaagtca taaaggattg 181 ctccgctttg ggtgggaacg acaagaacga cagcaagtaa aagaacagga tgacgcgcaa 241 tctcagacca acccagccga aggcggtcat ttgtgcctcc tcattcgtcc cagcatctcg 301 gcgcctccat aaaaccaaaa actaccacaa aaataagctg atagatcact gcgtgcttaa 361 aggcactata actaatatat cggataccat aactacccac cacatctagt ctggtatggt 421 tgggcgctaa ccggataagc acggatcact gttcatcagt aaggattcta tcttccggtg 481 aatcgttgcc cgccacatac acgaagacta gcaaggttcc atctataaat tccatataaa 541 atacatataa tttgtctata aatttggatc gagagagtgc gtgtcccgca tgaaacggcc 601 cgaggccagt ccatcaccgc ctacctgtgg ggcgaactgg ctgtgctgac ctgcccctgc 661 cacctcttca ttgccgtcgt gctggcaggt acgacgccgg cacgttcctc ggtgagcatt 721 tgggttatcg cggcgctcgg tttgaccgac ttgttccttc tgtcgctgtc gctggcgttg 781 cgggcatcag gcccttgact ccgtaccctg gtacggcagt aagttgggcc tacccaaccc 841 ctataataag cttatatcgt gatgacatag cgtgatgacc aggaggatct gtccatgtca 901 gccataaccc gcatcatcga caaaattggc atagtcggta ccatcgtcgg tagtttcagt 961 tgcgccatgt gtttccccgc agcagcgagc ctcggcgctg caatcggatt gggctttctc 1021 agccagtggg aaggcctgtt cgtgcagtgg ctgattccga ttttcgccag cgtggcatta 1081 ttggcgacct tggcgggctg gttctcgcac cgccaatggc aacgcacgct gctgggctcg 1141 atcggtccgg tgctagcgct tgtcggggtg tttgggttaa cgcatcactt tctggacaag 1201 gacctggcgc gcgtaatttt ttataccgga ttggtggtga tgttccttgt ctccatctgg 1261 gacatggtca atccggcgaa ccgctgcgcg accgacggct gcgaaacgcc cgccccgcgt 1321 agctgagcac atagacactt tggaggatat tatgaccgag aacgcgccca ccgaactcgc 1381 tatcactggc atgacctgcg acggttgcgc cgcgcatgtg cgcaaagcac tcgaaggcgt 1441 gcccggcgta cgcgaggcgc aggtgtccta cccggatgcc acggcccggg tcgtgctgga 1501 gggcgaggtg ccgatgcagc ggctaatcaa ggcggtggtt gcaagtggct atggtgtgca 1561 tccacggagc gacggtgcct cctccacaaa cgatggacag gagctacaca tcgctgtgat 1621 cggcaccggc ggagcggcga tggcgtgcgc attgaaggct gtcgagcggg gcgcgcgcgt 1681 gacgctgatc gaacgcagca ccatcggcgg cacctgcgtg aacatcggtt gcgtgccgtc 1741 caagatcatg atccgcgccg cccatatcgc ccacctccgc cgggaaagcc cattcgatgg 1801 cggcatccag gcggtcgcgc cgaccatcca gcgcacagcg ctgctggtcc aacagcaggc 1861 ccgtgtcgat gaactgcgtc acgccaagta cgaaggcatc ctggacggca acccggccat 1921 caccgttctg cgcggtgaag cgcgtttcaa ggacagccgg agtgttgtcg tccatttgaa 1981 cgatggtggc gagcgcgtcg taatgttcga ccgctgcctg gttgccacgg gcgccagtcc 2041 ggccgtgccg ccgattcccg gcttgaaaga cactccttat tggacctcca ccgaagggct 2101 ggtcagcgaa tcgatccccg agcgtctggc cgtgatcggc tcgtcggtgg tggcgctgga 2161 actggcgcaa gccttcgccc ggctcggcag ccatgtgacg atcctggcgc gcggcacctt 2221 gttcctccgg gaagacccgg ccatcggtga ggccatcacg gcggcgtttc gcgccgaagg 2281 catcgaggtg ctggagcaca cccaggccag ccaggtcgct tatgcggatg gcgaatttgt 2341 gctagccacc gggcacggcg aactgcgcgc cgataagctg ctggtcgcca ctggtcgcgc 2401 accgaacaca cgccgcctga atctggaagc ggcgggcgtg gccatcaatg cgcaaggggc 2461 catcgtcatc gaccagggta tgcgcacgaa cagcccgaac atttacgccg ctggcgactg 2521 caccgaccag ccgcaattcg tctacgtggc ggcagcggcc ggcacccgtg cggccatcaa 2581 catgatgggc ggtagtgcag ccctggactt gacggcgatg ccagccgtgg tgttcaccga 2641 tccgcaagtg gcgactgtgg gttacagcgc ggaagcgcat cgcgacggca tcgaaaccga 2701 cagccgcatg acgctcgaca acgtgccgcg ggcgctcgcc aatttcaata cacgcggctt 2761 catcaagctg gtagccgaag tgggcagtgg ctcgctaatc ggcgtgcagg tggtcgcccc 2821 ggaagcgggc gagctgatcc agactgccgc gctggcgatt cgtaaccgga tgacggtaca 2881 ggaactggct gaccagttgt ttccctacct gacgatggtc gaagggctga agcttgctgc 2941 ccagaccttc accagggatg tgaagcagtt gtcctgctgt gcgggttgag acggattgat 3001 aaaggagtcc ctgttgc //
GenBank-Updates@genbank.bio.net (01/30/91)
LOCUS TFEMERAC 3017 bp ds-DNA BCT 30-JAN-1991 DEFINITION T.ferrooxidans mer operon. ACCESSION D90110 M32353 KEYWORDS merA gene; merC gene; mercuric reductase; mercury resistance. SOURCE T.ferrooxidans (strain E-15) DNA; clones pTM31[4,5]. ORGANISM Thiobacillus ferrooxidans Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Colorless sulfur bacteria. REFERENCE 1 (bases 1379 to 3017) AUTHORS Inoue,C., Sugawara,K., Shiratori,T., Kusano,T. and Kitagawa,Y. TITLE Nucleotide sequence of the Thiobacillus ferrooxidans chromosomal gene encoding mercuric reductase JOURNAL Gene 84, 47-54 (1989) STANDARD simple staff_review REFERENCE 2 (bases 1 to 1378) AUTHORS Inoue,C., Sugawara,K. and Kusano,T. TITLE Thiobacillus ferrooxidans mer operon: Sequence analysis of the promoter and adjacent genes JOURNAL Gene 96, 115-120 (1990) STANDARD full staff_entry COMMENT These data kindly submitted in computer readable form by: Tomonobu Kusano Biotechnology Institute Akita Prefectural college of Agriculture Ohgata, Minami-Akitagun Akita 010-04 Japan Phone: 0185-45-2026 x403 Fax: 0185-45-2678 FEATURES Location/Qualifiers CDS 577..798 /note="URF-1" misc_signal 795..800 /note="-35 region" misc_signal 820..825 /note="-10 region" CDS 895..1326 /note="merC" CDS 1352..2989 /note="mercuric reductase (merA)" BASE COUNT 626 a 889 c 904 g 598 t ORIGIN 1 gtcgacaggt actccggtca gacgatatat tcttttttcc agcggtccca ggaaacgggt 61 ggcccagaat cgatgacctt caacaacacg atacatatag tggcccaacg gcaatgccag 121 aaaaactgac aatgccagaa ctgcaacaag gagaatgatt gcagaagtca taaaggattg 181 ctccgctttg ggtgggaacg acaagaacga cagcaagtaa aagaacagga tgacgcgcaa 241 tctcagacca acccagccga aggcggtcat ttgtgcctcc tcattcgtcc cagcatctcg 301 gcgcctccat aaaaccaaaa actaccacaa aaataagctg atagatcact gcgtgcttaa 361 aggcactata actaatatat cggataccat aactacccac cacatctagt ctggtatggt 421 tgggcgctaa ccggataagc acggatcact gttcatcagt aaggattcta tcttccggtg 481 aatcgttgcc cgccacatac acgaagacta gcaaggttcc atctataaat tccatataaa 541 atacatataa tttgtctata aatttggatc gagagagtgc gtgtcccgca tgaaacggcc 601 cgaggccagt ccatcaccgc ctacctgtgg ggcgaactgg ctgtgctgac ctgcccctgc 661 cacctcttca ttgccgtcgt gctggcaggt acgacgccgg cacgttcctc ggtgagcatt 721 tgggttatcg cggcgctcgg tttgaccgac ttgttccttc tgtcgctgtc gctggcgttg 781 cgggcatcag gcccttgact ccgtaccctg gtacggcagt aagttgggcc tacccaaccc 841 ctataataag cttatatcgt gatgacatag cgtgatgacc aggaggatct gtccatgtca 901 gccataaccc gcatcatcga caaaattggc atagtcggta ccatcgtcgg tagtttcagt 961 tgcgccatgt gtttccccgc agcagcgagc ctcggcgctg caatcggatt gggctttctc 1021 agccagtggg aaggcctgtt cgtgcagtgg ctgattccga ttttcgccag cgtggcatta 1081 ttggcgacct tggcgggctg gttctcgcac cgccaatggc aacgcacgct gctgggctcg 1141 atcggtccgg tgctagcgct tgtcggggtg tttgggttaa cgcatcactt tctggacaag 1201 gacctggcgc gcgtaatttt ttataccgga ttggtggtga tgttccttgt ctccatctgg 1261 gacatggtca atccggcgaa ccgctgcgcg accgacggct gcgaaacgcc cgccccgcgt 1321 agctgagcac atagacactt tggaggatat tatgaccgag aacgcgccca ccgaactcgc 1381 tatcactggc atgacctgcg acggttgcgc cgcgcatgtg cgcaaagcac tcgaaggcgt 1441 gcccggcgta cgcgaggcgc aggtgtccta cccggatgcc acggcccggg tcgtgctgga 1501 gggcgaggtg ccgatgcagc ggctaatcaa ggcggtggtt gcaagtggct atggtgtgca 1561 tccacggagc gacggtgcct cctccacaaa cgatggacag gagctacaca tcgctgtgat 1621 cggcaccggc ggagcggcga tggcgtgcgc attgaaggct gtcgagcggg gcgcgcgcgt 1681 gacgctgatc gaacgcagca ccatcggcgg cacctgcgtg aacatcggtt gcgtgccgtc 1741 caagatcatg atccgcgccg cccatatcgc ccacctccgc cgggaaagcc cattcgatgg 1801 cggcatccag gcggtcgcgc cgaccatcca gcgcacagcg ctgctggtcc aacagcaggc 1861 ccgtgtcgat gaactgcgtc acgccaagta cgaaggcatc ctggacggca acccggccat 1921 caccgttctg cgcggtgaag cgcgtttcaa ggacagccgg agtgttgtcg tccatttgaa 1981 cgatggtggc gagcgcgtcg taatgttcga ccgctgcctg gttgccacgg gcgccagtcc 2041 ggccgtgccg ccgattcccg gcttgaaaga cactccttat tggacctcca ccgaagggct 2101 ggtcagcgaa tcgatccccg agcgtctggc cgtgatcggc tcgtcggtgg tggcgctgga 2161 actggcgcaa gccttcgccc ggctcggcag ccatgtgacg atcctggcgc gcggcacctt 2221 gttcctccgg gaagacccgg ccatcggtga ggccatcacg gcggcgtttc gcgccgaagg 2281 catcgaggtg ctggagcaca cccaggccag ccaggtcgct tatgcggatg gcgaatttgt 2341 gctagccacc gggcacggcg aactgcgcgc cgataagctg ctggtcgcca ctggtcgcgc 2401 accgaacaca cgccgcctga atctggaagc ggcgggcgtg gccatcaatg cgcaaggggc 2461 catcgtcatc gaccagggta tgcgcacgaa cagcccgaac atttacgccg ctggcgactg 2521 caccgaccag ccgcaattcg tctacgtggc ggcagcggcc ggcacccgtg cggccatcaa 2581 catgatgggc ggtagtgcag ccctggactt gacggcgatg ccagccgtgg tgttcaccga 2641 tccgcaagtg gcgactgtgg gttacagcgc ggaagcgcat cgcgacggca tcgaaaccga 2701 cagccgcatg acgctcgaca acgtgccgcg ggcgctcgcc aatttcaata cacgcggctt 2761 catcaagctg gtagccgaag tgggcagtgg ctcgctaatc ggcgtgcagg tggtcgcccc 2821 ggaagcgggc gagctgatcc agactgccgc gctggcgatt cgtaaccgga tgacggtaca 2881 ggaactggct gaccagttgt ttccctacct gacgatggtc gaagggctga agcttgctgc 2941 ccagaccttc accagggatg tgaagcagtt gtcctgctgt gcgggttgag acggattgat 3001 aaaggagtcc ctgttgc //