GenBank-Updates@genbank.bio.net (12/05/90)
LOCUS BCICHIA3 2400 bp ds-DNA BCT 05-DEC-1990
DEFINITION B.circulans chitinase A1 (chiA) gene, complete cds.
ACCESSION M57601 J05599
KEYWORDS chitinase A1.
SOURCE Bacillus circulans (strain WL-12) DNA.
ORGANISM Bacillus circulans
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 2400)
AUTHORS Watanabe,T., Suzuki,K., Oyanagi,W., Ohnishi,K. and Tanaka,H.
TITLE Gene cloning of chitinase A1 from Bacillus circulans WL-12 revealed
its evolutionary relationship to Serratia chitinase and to the type
III homology units of fibronectin
JOURNAL J. Biol. Chem. 265, 15659-15665 (1990)
STANDARD simple staff_entry
FEATURES from to/span description
signal 47 52 35_signal
signal 69 74 10_signal
signal 222 229 ribosome binding site
pept 241 2340 chitinase A1 precursor /EC_number="3.2.1.14"
sigp 241 360 chitinase A1 signal peptide
matp 361 2337 chitinase A1
BASE COUNT 665 a 583 c 615 g 537 t
ORIGIN
1 agcggctgga ggcggctata cggcgccagc tgagctgcaa aaggcattga attatctgat
61 caaaggagta tcatacggcg gttcctatac cttgcgccag ctgcgggcta tgtcggtctc
121 aagggcatta tgacctggtc aatcaactgg gacgcgtata cgaataacca gttctcgaac
181 gcacatcgtc cattcctgaa tgggcttagc acgcaaaaga cagaggaggt tgtgtattaa
241 atgataaatt taaataaaca cactgctttt aagaagactg caaagttttt ccttggtctg
301 tccctgctct tatccgtcat cgttccttcc ttcgcgctcc aacctgctac ggccgaagca
361 gcagattctt ataaaatcgt tggttactat ccgtcctggg ctgcgtacgg cagaaactat
421 aatgtagccg atatcgatcc gaccaaagtg acgcatatca actatgcttt tgctgatatt
481 tgctggaacg gcattcatgg aaatccggac ccttcgggcc ccaatcctgt aacctggacc
541 tgtcagaatg aaaaaagcca aacgatcaat gtgccaaacg gaacaatcgt gctcggcgat
601 ccatggatcg atacgggcaa gacatttgca ggggatacgt gggatcagcc cattgcaggc
661 aatatcaatc agcttaacaa gttgaaacaa accaatccta acctgaagac tattatctcc
721 gttggaggat ggacgtggtc caaccgtttc tctgatgtag ccgcgactgc tgcaacccga
781 gaggtctttg cgaactctgc cgtcgatttc ctacggaagt acaattttga cggggtagat
841 ctggactggg agtacccggt atcaggcgga ctcgatggta acagcaaacg tcctgaagat
901 aagcaaaact acacattgct cttgagcaaa atccgtgaaa agctggatgc agcgggagct
961 gtggacggca agaagtatct gcttacgatt gcaagcggtg cgtctgcgac ctatgctgcc
1021 aatacggagc ttgcaaaaat tgctgctatc gtcgactgga ttaacattat gacatacgat
1081 tttaacgggg cttggcaaaa aatcagcgca cataatgcgc cgttgaacta tgatcctgcg
1141 gcgtcagccg ctggcgtgcc agatgccaat acatttaatg tggctgccgg agcacaaggg
1201 catttggatg caggcgtacc ggccgctaaa cttgtgcttg gtgttccatt ctatggccgt
1261 ggctgggatg gatgcgcaca ggcaggcaac ggccagtatc agacgtgcac gggtggttct
1321 tccgttggga catgggaagc aggatccttt gacttctatg atctggaagc gaattacatc
1381 aacaaaaacg gatacacgcg ttactggaat gacacggcca aagtgccata tctctataat
1441 gcgtccaata agcgctttat cagttatgac gatgcggagt ccgttggata taaaactgct
1501 tatatcaaga gcaaaggact cggcggagcg atgttctggg agctcagcgg tgaccgtaac
1561 aaaacactcc aaaacaaact gaaggccgat ctgcctaccg gaggtacagt gccaccagta
1621 gatacgacag caccaagcgt accagggaat gcccgttcga caggcgtgac agcaaactcg
1681 gtgacgctgg cctggaatgc ttcaacggat aatgtaggcg ttaccggtta taacgtctat
1741 aatggcgcta atcttgcgac atccgtcacc ggaacgacag caacaattag tggacttaca
1801 gcggggacct catatacctt cacgataaaa gcaaaagatg ccgcaggcaa tctgtctgca
1861 gccagtaatg ctgtaactgt aagcacaaca gctcagccgg gaggcgatac gcaagcacca
1921 accgcaccaa cgaaccttgc gtcgaccgcc caaacaacat ccagcataac actgagctgg
1981 actgcatcca ctgataatgt tggtgtaacg ggttatgacg tatacaacgg aacggcactg
2041 gcaacaacgg tgactggaac gacggcaacg atcagcgggc ttgctgcgga tacctcgtat
2101 acatttacgg taaaagcaaa ggatgcggca ggcaacgtgt ctgcagcgag caacgcggtg
2161 agtgtgaaga cagccgccga aacgacaaat cctggtgtat ccgcttggca ggtcaacaca
2221 gcttatactg cgggacaatt ggtcacatat aacggcaaga cgtataaatg tttgcagccc
2281 cacacctcct tggcaggatg ggaaccatcc aacgttcctg ccttgtggca gcttcaatag
2341 ttaaaaattg aaatacaaca tttttaattg aaatgtgcag tgttggcgag cttagtttag
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS BCICHIA3 2400 bp ds-DNA BCT 31-JAN-1991
DEFINITION B.circulans chitinase A1 (chiA) gene, complete cds.
ACCESSION M57601 J05599
KEYWORDS chitinase A1.
SOURCE Bacillus circulans (strain WL-12) DNA.
ORGANISM Bacillus circulans
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2400)
AUTHORS Watanabe,T., Suzuki,K., Oyanagi,W., Ohnishi,K. and Tanaka,H.
TITLE Gene cloning of chitinase A1 from Bacillus circulans WL-12 revealed
its evolutionary relationship to Serratia chitinase and to the type
III homology units of fibronectin
JOURNAL J. Biol. Chem. 265, 15659-15665 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
-35_signal 47..52
/gene="chiA"
-10_signal 69..74
/gene="chiA"
RBS 222..229
/gene="chiA"
CDS 241..2340
/EC_number="3.2.1.14"
/product="chitinase A1"
/gene="chiA"
/codon_start=241
sig_peptide 241..360
/gene="sTnC"
/codon_start=241
mat_peptide 361..2337
/product="chitinase A1"
/gene="chiA"
/codon_start=361
BASE COUNT 665 a 583 c 615 g 537 t
ORIGIN
1 agcggctgga ggcggctata cggcgccagc tgagctgcaa aaggcattga attatctgat
61 caaaggagta tcatacggcg gttcctatac cttgcgccag ctgcgggcta tgtcggtctc
121 aagggcatta tgacctggtc aatcaactgg gacgcgtata cgaataacca gttctcgaac
181 gcacatcgtc cattcctgaa tgggcttagc acgcaaaaga cagaggaggt tgtgtattaa
241 atgataaatt taaataaaca cactgctttt aagaagactg caaagttttt ccttggtctg
301 tccctgctct tatccgtcat cgttccttcc ttcgcgctcc aacctgctac ggccgaagca
361 gcagattctt ataaaatcgt tggttactat ccgtcctggg ctgcgtacgg cagaaactat
421 aatgtagccg atatcgatcc gaccaaagtg acgcatatca actatgcttt tgctgatatt
481 tgctggaacg gcattcatgg aaatccggac ccttcgggcc ccaatcctgt aacctggacc
541 tgtcagaatg aaaaaagcca aacgatcaat gtgccaaacg gaacaatcgt gctcggcgat
601 ccatggatcg atacgggcaa gacatttgca ggggatacgt gggatcagcc cattgcaggc
661 aatatcaatc agcttaacaa gttgaaacaa accaatccta acctgaagac tattatctcc
721 gttggaggat ggacgtggtc caaccgtttc tctgatgtag ccgcgactgc tgcaacccga
781 gaggtctttg cgaactctgc cgtcgatttc ctacggaagt acaattttga cggggtagat
841 ctggactggg agtacccggt atcaggcgga ctcgatggta acagcaaacg tcctgaagat
901 aagcaaaact acacattgct cttgagcaaa atccgtgaaa agctggatgc agcgggagct
961 gtggacggca agaagtatct gcttacgatt gcaagcggtg cgtctgcgac ctatgctgcc
1021 aatacggagc ttgcaaaaat tgctgctatc gtcgactgga ttaacattat gacatacgat
1081 tttaacgggg cttggcaaaa aatcagcgca cataatgcgc cgttgaacta tgatcctgcg
1141 gcgtcagccg ctggcgtgcc agatgccaat acatttaatg tggctgccgg agcacaaggg
1201 catttggatg caggcgtacc ggccgctaaa cttgtgcttg gtgttccatt ctatggccgt
1261 ggctgggatg gatgcgcaca ggcaggcaac ggccagtatc agacgtgcac gggtggttct
1321 tccgttggga catgggaagc aggatccttt gacttctatg atctggaagc gaattacatc
1381 aacaaaaacg gatacacgcg ttactggaat gacacggcca aagtgccata tctctataat
1441 gcgtccaata agcgctttat cagttatgac gatgcggagt ccgttggata taaaactgct
1501 tatatcaaga gcaaaggact cggcggagcg atgttctggg agctcagcgg tgaccgtaac
1561 aaaacactcc aaaacaaact gaaggccgat ctgcctaccg gaggtacagt gccaccagta
1621 gatacgacag caccaagcgt accagggaat gcccgttcga caggcgtgac agcaaactcg
1681 gtgacgctgg cctggaatgc ttcaacggat aatgtaggcg ttaccggtta taacgtctat
1741 aatggcgcta atcttgcgac atccgtcacc ggaacgacag caacaattag tggacttaca
1801 gcggggacct catatacctt cacgataaaa gcaaaagatg ccgcaggcaa tctgtctgca
1861 gccagtaatg ctgtaactgt aagcacaaca gctcagccgg gaggcgatac gcaagcacca
1921 accgcaccaa cgaaccttgc gtcgaccgcc caaacaacat ccagcataac actgagctgg
1981 actgcatcca ctgataatgt tggtgtaacg ggttatgacg tatacaacgg aacggcactg
2041 gcaacaacgg tgactggaac gacggcaacg atcagcgggc ttgctgcgga tacctcgtat
2101 acatttacgg taaaagcaaa ggatgcggca ggcaacgtgt ctgcagcgag caacgcggtg
2161 agtgtgaaga cagccgccga aacgacaaat cctggtgtat ccgcttggca ggtcaacaca
2221 gcttatactg cgggacaatt ggtcacatat aacggcaaga cgtataaatg tttgcagccc
2281 cacacctcct tggcaggatg ggaaccatcc aacgttcctg ccttgtggca gcttcaatag
2341 ttaaaaattg aaatacaaca tttttaattg aaatgtgcag tgttggcgag cttagtttag
//