GenBank-Updates@genbank.bio.net (11/28/90)
LOCUS BSUPEPFA 4654 bp ds-DNA BCT 28-NOV-1990
DEFINITION B.subtilis bacillopeptidase F (bpr) gene, complete cds.
ACCESSION M29035
KEYWORDS bacillopeptidase; serine protease.
SOURCE B.subtilis (168, strain GP216) DNA, clone pCR83.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 4654)
AUTHORS Sloma,A., Rufo,G.A.Jr., Rudolph,C.F., Sullivan,B.J.,
Theriault,K.A. and Pero,J.
TITLE Bacillopeptidase F of Bacillus subtilis: Purification of the
protein and cloning of the gene
JOURNAL J. Bacteriol. 172, 1470-1477 (1990)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Sloma,A., Rufo,G.A.Jr., Rudolph,C.F., Sullivan,B.J.,
Theriault,K.A. and Pero,J.
TITLE Errata: Bacillopeptidase F of Bacillus subtilis: Purfication of the
protein and cloning of the gene
JOURNAL J. Bacteriol. 172, 5520-5521 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol. 172,
1470-1477 (1990)] kindly submitted by A.Sloma, 14-OCT-1989.
FEATURES from to/span description
pept 200 4501 bacillopeptidase F precursor
sigp 200 289 bacillopeptidase F signal peptide
matp 786 2463 bacillopeptidase F
binding 186 197 ribosome binding site
BASE COUNT 1525 a 906 c 1133 g 1090 t
ORIGIN
1 taacaaacag ataattagac ccatttattt tgtgagattt tatcatttca tatatatgga
61 aattgaacga cacgaaacga caatatctgt aattcagatt gtctacagtt aatatacagc
121 gatgttctga caaaccattc attattaaaa ggagggacga cacttttttt aaaaagcatg
181 ttgaaaaagg gggatgaaaa tgaggaaaaa aacgaaaaac agactcatca gctctgtttt
241 aagtacagtt gtcatcagtt cactgctgtt tccgggagca gccggggcaa gcagtaaagt
301 cacctcacct tctgttaaaa aggagcttca atctgcggaa tccattcaaa acaagatttc
361 gagttcatta aagaaaagct ttaaaaagaa agaaaaaacg acttttctga ttaaatttaa
421 agatctggct aacccagaaa aagcggcaaa agcggctgtt aaaaaagcga aatcgaagaa
481 gctgtctgcc gctaagacgg aatatcaaaa gcgttctgct gttgtgtcat ctttaaaagt
541 cacagccgat gaatcccagc aagatgtcct aaaatacttg aacacccaga aagataaagg
601 aaatgcagac caaattcatt cttattatgt ggtgaacggg attgctgttc atgcctcaaa
661 agaggttatg gaaaaagtgg tgcagtttcc cgaagtggaa aaggtgcttc ctaatgagaa
721 acggcagctt tttaagtcat cctccccatt taatatgaaa aaagcacaga aagctattaa
781 agcaactgac ggtgtggaat ggaatgtaga ccaaatcgat gccccaaaag cttgggcact
841 tggatatgat ggaactggca cggttgttgc gtccattgat accggggtgg aatggaatca
901 tccggcatta aaagagaaat atcgcggata taatccggaa aatcctaatg agcctgaaaa
961 tgaaatgaac tggtatgatg ccgtagcagg cgaggcaagc ccttatgatg atttggctca
1021 tggaacccac gtgacaggca cgatggtggg ctctgaacct gatggaacaa atcaaatcgg
1081 tgtagcacct ggcgcaaaat ggattgctgt taaagcgttc tctgaagatg gcggcactga
1141 tgctgacatt ttggaagctg gtgaatgggt tttagcacca aaggacgcgg aaggaaatcc
1201 ccacccggaa atggctcctg atgttgtcaa taactcatgg ggagggggct ctggacttga
1261 tgaatggtac agagacatgg tcaatgcctg gcgtgcggcc gatattttcc ctgagttttc
1321 agcggggaat acggatctct ttattcccgg cgggcctggt tctatcgcaa atccggcaaa
1381 ctatccagaa tcgtttgcaa ctggagcgac tgatatcaat aaaaagctcg ctgacttttc
1441 tcttcaaggg ccatctccat atgatgaaat aaagccggaa atatctgcac cgggcgttaa
1501 tattcgttca tccgttcccg gtcagacata tgaggatggt tgggacggca catcaatggc
1561 agggccgcat gtatccgctg ttgctgcact gctgaaacag gcgaatgcct cactttctgt
1621 tgatgagatg gaggatatat taaccagcac ggctgaaccg ctcacggatt caacatttcc
1681 tgattcaccg aataacggat atggccatgg tctggtgaat gcttttgatg ctgtatccgc
1741 tgttacagat ggattaggga aagcggaagg acaagtttct gtagaggggg atgaccaaga
1801 gcctcctgtc tatcagcatg agaaagtaac tgaagcttat gaaggtggca gcctaccact
1861 gactttgaca gctgaagaca atgtgagtgt gacatctgta aagctgtcct acaagcttga
1921 tcaaggtgaa tggacagaaa taacggctaa acgaatcagc ggtgatcatc taaaaggaac
1981 gtatcaggca gagatcccag atataaaagg aactaaacta agctataagt ggatgattca
2041 cgattttggc ggtcatgtcg tttcgtctga cgtatacgat gtaacagtga aaccaagcat
2101 cacggcggga tataagcagg actttgaaac tgcacccggc ggctgggttg cgagcggaac
2161 aaataataac tgggaatggg gagttccgtc aactggccca aatacagcag catccggaga
2221 aaaagtatat ggaacgaatt tgacaggaaa ttatgccaac tcagcaaaca tgaaccttgt
2281 tatgcctcct attaaagcac ctgattcagg aagtctgttc cttcaattta aaagctggca
2341 caatttagag gatgattttg attacggcta cgtttttgtt cttccggaag gtgaaaagaa
2401 ttgggagcag gctggtgtct ataacggaaa gacatcaagc tggacggacg aagaaattga
2461 tttatcggct tataaaggcc aaaacattca agtgatgttt aaccttcaat ctgatgaaag
2521 cattgcaaaa gagggctggt atattgatga tgtagtgctt tctgacaaat cagccggaaa
2581 aacagtcaaa aagaataagc tgggcgtcga aaagccgtct ggaaagcaaa agaaaaagcc
2641 agtaaatccg aaaaaggcta agccatctgc aaacacagcg gtaaaacatc agaacaaggc
2701 tatacagcct caagttttgc cgctcaaggc acaagtcagt gtagtggaaa caggaaaatc
2761 aacatattca gatcaatcca cagggcagta cacgctgaag cacaaagcgg gagactatac
2821 gcttatggca gaagcatatg gttatcagtc gaaaacacaa aaagtatctt taaagacgga
2881 tcagacgaca caagctaatt ttacgttaga agagatgaag aagggcacat taaaaggcac
2941 ggtcatcaat aaaacgacag gagagccggt aacaggcgct tccgtttatg ttgtagagga
3001 tgctgcggtg gaaccggcta tgacaaacga caaaggtgaa tatatgctgg aggcctatga
3061 aggcgcttat acgattaaag tcgctgcacc gggttattac agtgatgaat tttccgttga
3121 gttaaaaggt gatgttacaa aagaaactgc attgaagcct ttcgtcggtt atccgggtga
3181 aattgcatat gatgacggaa cagcggagaa tgccaattcc tattttgctg ccggtaacgg
3241 atgggcggta aaaatgacgc tcgctgacgg caaggacaaa ggcatgctta caggagggct
3301 gttcagattc tgggatacag agttccctga tccgggcggc acagagttta aggttgaggt
3361 atacgatgcc acaggaaaag acggagcacc gggcaagaaa attgcagggc catttaacgc
3421 ggaagctctt cgcaatggcg agtggactaa ggtagatctc agttcaaaag ggattatggt
3481 cgataaagat ttttatctcg tatatatcca gtcgaaacct gatccgtatt cacctggatt
3541 ggcaatggat gaaaccggcc agaattccgg ccgcaactgg cagtatatag atggaaagtg
3601 gcagccaggt gacaaagcgg atggcaacta tatgattcgc gcattagttg attatgaagc
3661 tgctgtacct gagattactt caccgacaga caaatcatac acaaataagg atagcgtcac
3721 tgtaaaagga aacgcttctc ctggcacaac ggtacacatt tataatggag agaaagaagc
3781 aggagaaacg aaagctgctg cggatggcac gttccatgca ggcatcatac tcaacaaggg
3841 tgaaaatgag ctgacggcaa ctgcatcaac tgacaacgga acaacagatg cctccagccc
3901 aatcacggtc acgcttgatc aagaaaagcc tgaattaaca ctggacaatc caaaggatgg
3961 cgggaaaaca aataaagaaa cgctgactgt caaaggggct gtatccgatg acaatctgaa
4021 agacgtcaag gtgaatggca aaaaagcaac agtagctgat ggttcatact cagcccgtat
4081 tcttttggaa aatggaagaa atgaaatcaa ggtaattgct acagacttgg caggcaacaa
4141 aacgacgaaa aagacagtca ttgatgtgaa ctttgacaag cctgtcattt ccggcttaat
4201 tccgggagag gataaaaact taaaagccgg tgaatctgtg aaaatcgctt tctcaagcgc
4261 tgaggattta gatgcaacgt ttaccattcg tatgccgctg accaatgcaa gagcgagtgt
4321 gcaaaatgcc accgaactcc cgttaagaga aatctctccg gggagatatg aaggctattg
4381 gactgccact tcttctatta aagcaaaagg agcaaaagta gaagtgatcg tccgagatga
4441 ttatggaaat gaaacaagaa aaactgcgaa tggaaaactt aatatgaaca cagaaaatta
4501 agtggaaaaa aagctgccgt cattggcagc tttttttata tcctctcatt atacttcctc
4561 gacaaattaa gcagatttcc ctgaaaaatt gtattttcct ctcaacatta attgacagac
4621 tttcccacag agcttgcttt atacttatga agca
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS BSUPEPFA 4654 bp ds-DNA BCT 31-JAN-1991
DEFINITION B.subtilis bacillopeptidase F (bpr) gene, complete cds.
ACCESSION M29035
KEYWORDS bacillopeptidase; serine protease.
SOURCE B.subtilis (168, strain GP216) DNA, clone pCR83.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4654)
AUTHORS Sloma,A., Rufo,G.A., Rudolph,C.F., Sullivan,B.J., Theriault,K.A.
and Pero,J.
TITLE Bacillopeptidase F of Bacillus subtilis: Purification of the
protein and cloning of the gene
JOURNAL J. Bacteriol. 172, 1470-1477 (1990)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Sloma,A., Rufo,G.A.Jr., Rudolph,C.F., Sullivan,B.J., Theriault,K.A.
and Pero,J.
TITLE Errata: Bacillopeptidase F of Bacillus subtilis: Purfication of the
protein and cloning of the gene
JOURNAL J. Bacteriol. 172, 5520-5521 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol. 172,
1470-1477 (1990)] kindly submitted
by A.Sloma, 14-OCT-1989.
FEATURES Location/Qualifiers
misc_binding 186..197
/note="ribosome binding site"
CDS 200..4501
/note="bacillopeptidase F precursor"
/product="bacillopeptidase F"
sig_peptide 200..289
/note="bacillopeptidase F signal peptide"
mat_peptide 786..2463
/note="bacillopeptidase F"
BASE COUNT 1525 a 906 c 1133 g 1090 t
ORIGIN
1 taacaaacag ataattagac ccatttattt tgtgagattt tatcatttca tatatatgga
61 aattgaacga cacgaaacga caatatctgt aattcagatt gtctacagtt aatatacagc
121 gatgttctga caaaccattc attattaaaa ggagggacga cacttttttt aaaaagcatg
181 ttgaaaaagg gggatgaaaa tgaggaaaaa aacgaaaaac agactcatca gctctgtttt
241 aagtacagtt gtcatcagtt cactgctgtt tccgggagca gccggggcaa gcagtaaagt
301 cacctcacct tctgttaaaa aggagcttca atctgcggaa tccattcaaa acaagatttc
361 gagttcatta aagaaaagct ttaaaaagaa agaaaaaacg acttttctga ttaaatttaa
421 agatctggct aacccagaaa aagcggcaaa agcggctgtt aaaaaagcga aatcgaagaa
481 gctgtctgcc gctaagacgg aatatcaaaa gcgttctgct gttgtgtcat ctttaaaagt
541 cacagccgat gaatcccagc aagatgtcct aaaatacttg aacacccaga aagataaagg
601 aaatgcagac caaattcatt cttattatgt ggtgaacggg attgctgttc atgcctcaaa
661 agaggttatg gaaaaagtgg tgcagtttcc cgaagtggaa aaggtgcttc ctaatgagaa
721 acggcagctt tttaagtcat cctccccatt taatatgaaa aaagcacaga aagctattaa
781 agcaactgac ggtgtggaat ggaatgtaga ccaaatcgat gccccaaaag cttgggcact
841 tggatatgat ggaactggca cggttgttgc gtccattgat accggggtgg aatggaatca
901 tccggcatta aaagagaaat atcgcggata taatccggaa aatcctaatg agcctgaaaa
961 tgaaatgaac tggtatgatg ccgtagcagg cgaggcaagc ccttatgatg atttggctca
1021 tggaacccac gtgacaggca cgatggtggg ctctgaacct gatggaacaa atcaaatcgg
1081 tgtagcacct ggcgcaaaat ggattgctgt taaagcgttc tctgaagatg gcggcactga
1141 tgctgacatt ttggaagctg gtgaatgggt tttagcacca aaggacgcgg aaggaaatcc
1201 ccacccggaa atggctcctg atgttgtcaa taactcatgg ggagggggct ctggacttga
1261 tgaatggtac agagacatgg tcaatgcctg gcgtgcggcc gatattttcc ctgagttttc
1321 agcggggaat acggatctct ttattcccgg cgggcctggt tctatcgcaa atccggcaaa
1381 ctatccagaa tcgtttgcaa ctggagcgac tgatatcaat aaaaagctcg ctgacttttc
1441 tcttcaaggg ccatctccat atgatgaaat aaagccggaa atatctgcac cgggcgttaa
1501 tattcgttca tccgttcccg gtcagacata tgaggatggt tgggacggca catcaatggc
1561 agggccgcat gtatccgctg ttgctgcact gctgaaacag gcgaatgcct cactttctgt
1621 tgatgagatg gaggatatat taaccagcac ggctgaaccg ctcacggatt caacatttcc
1681 tgattcaccg aataacggat atggccatgg tctggtgaat gcttttgatg ctgtatccgc
1741 tgttacagat ggattaggga aagcggaagg acaagtttct gtagaggggg atgaccaaga
1801 gcctcctgtc tatcagcatg agaaagtaac tgaagcttat gaaggtggca gcctaccact
1861 gactttgaca gctgaagaca atgtgagtgt gacatctgta aagctgtcct acaagcttga
1921 tcaaggtgaa tggacagaaa taacggctaa acgaatcagc ggtgatcatc taaaaggaac
1981 gtatcaggca gagatcccag atataaaagg aactaaacta agctataagt ggatgattca
2041 cgattttggc ggtcatgtcg tttcgtctga cgtatacgat gtaacagtga aaccaagcat
2101 cacggcggga tataagcagg actttgaaac tgcacccggc ggctgggttg cgagcggaac
2161 aaataataac tgggaatggg gagttccgtc aactggccca aatacagcag catccggaga
2221 aaaagtatat ggaacgaatt tgacaggaaa ttatgccaac tcagcaaaca tgaaccttgt
2281 tatgcctcct attaaagcac ctgattcagg aagtctgttc cttcaattta aaagctggca
2341 caatttagag gatgattttg attacggcta cgtttttgtt cttccggaag gtgaaaagaa
2401 ttgggagcag gctggtgtct ataacggaaa gacatcaagc tggacggacg aagaaattga
2461 tttatcggct tataaaggcc aaaacattca agtgatgttt aaccttcaat ctgatgaaag
2521 cattgcaaaa gagggctggt atattgatga tgtagtgctt tctgacaaat cagccggaaa
2581 aacagtcaaa aagaataagc tgggcgtcga aaagccgtct ggaaagcaaa agaaaaagcc
2641 agtaaatccg aaaaaggcta agccatctgc aaacacagcg gtaaaacatc agaacaaggc
2701 tatacagcct caagttttgc cgctcaaggc acaagtcagt gtagtggaaa caggaaaatc
2761 aacatattca gatcaatcca cagggcagta cacgctgaag cacaaagcgg gagactatac
2821 gcttatggca gaagcatatg gttatcagtc gaaaacacaa aaagtatctt taaagacgga
2881 tcagacgaca caagctaatt ttacgttaga agagatgaag aagggcacat taaaaggcac
2941 ggtcatcaat aaaacgacag gagagccggt aacaggcgct tccgtttatg ttgtagagga
3001 tgctgcggtg gaaccggcta tgacaaacga caaaggtgaa tatatgctgg aggcctatga
3061 aggcgcttat acgattaaag tcgctgcacc gggttattac agtgatgaat tttccgttga
3121 gttaaaaggt gatgttacaa aagaaactgc attgaagcct ttcgtcggtt atccgggtga
3181 aattgcatat gatgacggaa cagcggagaa tgccaattcc tattttgctg ccggtaacgg
3241 atgggcggta aaaatgacgc tcgctgacgg caaggacaaa ggcatgctta caggagggct
3301 gttcagattc tgggatacag agttccctga tccgggcggc acagagttta aggttgaggt
3361 atacgatgcc acaggaaaag acggagcacc gggcaagaaa attgcagggc catttaacgc
3421 ggaagctctt cgcaatggcg agtggactaa ggtagatctc agttcaaaag ggattatggt
3481 cgataaagat ttttatctcg tatatatcca gtcgaaacct gatccgtatt cacctggatt
3541 ggcaatggat gaaaccggcc agaattccgg ccgcaactgg cagtatatag atggaaagtg
3601 gcagccaggt gacaaagcgg atggcaacta tatgattcgc gcattagttg attatgaagc
3661 tgctgtacct gagattactt caccgacaga caaatcatac acaaataagg atagcgtcac
3721 tgtaaaagga aacgcttctc ctggcacaac ggtacacatt tataatggag agaaagaagc
3781 aggagaaacg aaagctgctg cggatggcac gttccatgca ggcatcatac tcaacaaggg
3841 tgaaaatgag ctgacggcaa ctgcatcaac tgacaacgga acaacagatg cctccagccc
3901 aatcacggtc acgcttgatc aagaaaagcc tgaattaaca ctggacaatc caaaggatgg
3961 cgggaaaaca aataaagaaa cgctgactgt caaaggggct gtatccgatg acaatctgaa
4021 agacgtcaag gtgaatggca aaaaagcaac agtagctgat ggttcatact cagcccgtat
4081 tcttttggaa aatggaagaa atgaaatcaa ggtaattgct acagacttgg caggcaacaa
4141 aacgacgaaa aagacagtca ttgatgtgaa ctttgacaag cctgtcattt ccggcttaat
4201 tccgggagag gataaaaact taaaagccgg tgaatctgtg aaaatcgctt tctcaagcgc
4261 tgaggattta gatgcaacgt ttaccattcg tatgccgctg accaatgcaa gagcgagtgt
4321 gcaaaatgcc accgaactcc cgttaagaga aatctctccg gggagatatg aaggctattg
4381 gactgccact tcttctatta aagcaaaagg agcaaaagta gaagtgatcg tccgagatga
4441 ttatggaaat gaaacaagaa aaactgcgaa tggaaaactt aatatgaaca cagaaaatta
4501 agtggaaaaa aagctgccgt cattggcagc tttttttata tcctctcatt atacttcctc
4561 gacaaattaa gcagatttcc ctgaaaaatt gtattttcct ctcaacatta attgacagac
4621 tttcccacag agcttgcttt atacttatga agca
//