GenBank-Updates@genbank.bio.net (11/30/90)
LOCUS BSUPYDHY 9019 bp ds-DNA BCT 30-NOV-1990
DEFINITION B.subtilis pyruvate dehydrogenase complex genes, complete cds.
ACCESSION M57435 M31542
KEYWORDS dihydrolipoamide acetyltransferase E2 subunit;
dihydrolipoamide dehydrogenase E3 subunit;
pyruvate decarboxylase E1-alpha subunit;
pyruvate decarboxylase E1-beta subunit;
pyruvate dehydrogenase complex.
SOURCE B.subtilis (strain 168) BRB1 (sacA321 metB5) cell line DNA, clones
sc2 and sc4.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 9019)
AUTHORS Hemila,H., Palva,A., Paulin,L., Arvidson,S. and Palva,I.
TITLE Secretory S complex of Bacillus subtilis: Sequence analysis and
identity to pyruvate dehydrogenase
JOURNAL J. Bacteriol. 172, 5052-5063 (1990)
STANDARD full staff_review
FEATURES from to/span description
pept 540 1253 ORF5
signal 1548 1553 /new_qualifier="-35_signal"
signal 1573 1578 /new_qualifier="-10_signal"
rpt 1598 1611 /rpt_type="inverted"
signal 1655 1666 /new_qualifier="RBS"
pept 1677 2792 pyruvate decarboxylase E1-alpha subunit
/EC_number="1.2.4.1"
signal 2778 2787 /new_qualifier="RBS"
pept 2796 3773 pyruvate decarboxylase E1-beta subunit
/EC_number="1.2.4.1"
signal 3742 3747 /new_qualifier="-35_signal"
signal 3769 3774 /new_qualifier="-10_signal"
rpt 3790 3815 /rpt_type="inverted"
signal 3873 3879 /new_qualifier="RBS"
pept 3888 5216 dihydrolipoamide acetyltransferase E2 subunit
/EC_number="2.3.1.12"
signal 5134 5139 /new_qualifier="-35_signal"
signal 5160 5165 /new_qualifier="-10_signal"
signal 5204 5213 /new_qualifier="RBS"
pept 5221 6633 dihydrolipoamide dehydrogenase E3 subunit
/EC_number="1.8.1.4"
pept > 9018 7630 (c) ORF6
BASE COUNT 2796 a 1795 c 1982 g 2446 t
ORIGIN
1 gctgctgttt ttctgcatca gttggaggca gctcaacagg ttcggcagtc tctctgaggg
61 ctggatggcc gtcacgtacg atgttttcca tagtaatcaa gaaacttcac tcctaaagct
121 taatatgtaa attatatcgg atttcttcac aaaggtgcaa cgaaacgaat gaccagccgt
181 atatggctgg tcaatttcat gctgtgtggt tagcaacagc ctccacctaa ataagcagct
241 ccgacaatga ttaacagaat gaataaaaca acgattagcg caaaggtgcg gccgtagcca
301 tatccgcagc caccatatcc gtaaccacca taactgcagc agcctccgcc gtaaccatat
361 ccatacatat tacataacct cctcttttaa ttacgccaga gcctttataa gtaggcatag
421 cgtctaatat aacctatgta aaaacaaaaa agttgtatgg tcatctgtcc tgatttttca
481 cttttttgat ttaatttcag tattgcattt atacagcacg gctattgcca aatgtcccca
541 tggattctat agtagaaatt gtcaagattg ggggatttaa cgtgaaagac gctataaaac
601 ggcggcctgg aatcaagatc ggagcttttt gtgccatggc actgatatta acaggatgca
661 tgggacagga cccggttgat tcacttcatc attcattaga aaaagccgcg gaatcggaaa
721 agccgtttca agaagaacaa aacacactca aagaattaga ggccaaagaa aatcagcttt
781 acgatagtgt tatgaagctc aatatggatg attataaaaa aattgttgct ttatctaata
841 aggcgcttga aaatgtcagc aagcacgaag aacacctaaa gcttgaaaat gacagcatga
901 aaaaatcgga aacagaattt gaggaagcaa aaacgtcagc ggaacatatt aaggaaaaag
961 acataaaaga aaaggccgat gccgcagcaa accatatgga gaaaagatat acatcatatg
1021 gcactatgta taaagagtat aaaaaagcgc ttgagcttaa taaaaaactg tatatgcagc
1081 tgaaagacaa agatttgaca agagatgatc tggatcagca gatcgataaa gtcaatgctt
1141 cttatgaaaa gatccttaaa tatagcggag aatttaatga acaaaccgaa aaatataata
1201 aagcccggga agatttatat gatgctgcag gctatcgtgt gaaaaagagc tgactcgatc
1261 agctcttttt ttgaatgaaa tgcgattcgg atttcgttca aaattttcat ttctattgta
1321 gtacagcata gtaaggtata ctagtacagt tttgggtgcg gttggcatct gtttttttga
1381 tgaaaatgta aagcgaatac atttattgaa aatcagcgat gaaaacgatt gacggttctt
1441 ctttatttgg tgtaaactaa tccatgttaa gcggtgttgt attagttttt tacaacttcg
1501 aaataataca gttcaaagca gagtgtaact atcctacata aaaggttttg gcaaactggg
1561 gatcggctaa aatataatac gacttactgc tgatacttta gggtacccta agtttgtatg
1621 tataaaagga atgcacgtct aatgactgtt taaaagaaag gaagaggtga cttagtatgg
1681 ctgcaaaaac gaaaaaagct atcgttgaca gtaagaagca atttgatgcc attaaaaagc
1741 agttcgaaac gttccaaatt ttaaatgaaa agggagaagt cgtgaatgaa gcggcgatgc
1801 ctgatttaac tgatgatcaa ttaaaagagc taatgcgccg catggtattt acgcgtgtgc
1861 ttgaccaacg ctctatctca ttaaaccgtc aaggacgtct cggattttac gctcctactg
1921 cgggtcaaga agcttctcag attgcaacgc atttcgcgct tgaaaaagaa gactttgttc
1981 ttcctggata ccgtgatgtg cctcagttaa tttggcacgg ccttccatta tatcaagcgt
2041 ttcttttctc tcgcggacat ttcagaggaa accaaatgcc tgatgatgtg aatgcccttt
2101 ctccacaaat cattatcggt gctcaataca ttcaaactgc cggtgttgcg ctaggtctta
2161 aaaaacgcgg taagaaagct gtcgcaatca cttacactgg tgacggcgga cgttctcaag
2221 gggacttcta cgaaggaatt aactttgccg gagcttataa agcacctgca atcttcgtgg
2281 tgcaaaacaa ccgttacgcg atttcaactc ctgttgaaaa acaatctgca gctgaaacaa
2341 ttgcacaaaa agctgtagct gccggtattg tcggtgtaca agtagacgga atggatccgc
2401 ttgctgtata cgcagcaact gctgaagcac gcgagcgcgc aatcaacggc gaaggtccaa
2461 cactaattga aacacttaca ttccgttatg gcccgcacac aatggctggt gacgatccta
2521 ctaaatatcg tacaaaagaa atcgaaaatg agtgggaaca aaaagatccg cttgtacgtt
2581 tccgtgcgtt ccttgaaaac aaaggcttat ggtctgaaga agaagaagca aaagtgattg
2641 aggatgcgaa agaagaaatt aagcaagcga tcaaaaaagc tgatgctgaa ccgaagcaaa
2701 aagtaactga tttaatgaaa atcatgtacg aaaaaatgcc tcacaacctt gaggagcaat
2761 ttgaaattta tacacagaag gagtcgaagt aagccatggc gcaaatgaca atgattcaag
2821 caatcacgga tgcgttacgc acagaactga aaaatgacga aaatgtctta gttttcggag
2881 aagacgttgg tgtaaacggc ggcgtattcc gtgcgacaga aggattgcaa aaagaattcg
2941 gtgaagaccg tgtgtttgac acgccacttg ctgaatctgg tatcggcggt cttgcccttg
3001 gtttaggctt aaacggcttc cgtccggtaa tggaaatcca attcttcgga tttgtttatg
3061 aagtaatgga ttcagtttct ggccaaatgg ctcgtatgcg ctaccgttct ggcggacgct
3121 ggacttcacc tgtaacaatt cgttctccat tcggcggcgg tgttcatact cctgaacttc
3181 acgctgacag cttggaaggt cttgttgcac aacagcctgg tatcaaagtt gttattccat
3241 caactcctta cgatgccaaa ggacttttaa tttctgcgat cagagacaat gatcctgttg
3301 tcttcttaga gcatatgaag ctttaccgtt ctttccgtca ggaagttcct gaagaagaat
3361 acacaattga gcttggaaaa gctgacgtga aacgtgaagg tactgatctt tcaatcatca
3421 cttacggcgc aatggttcat gaatcattaa aagctgctga tgagcttgaa aaagacggca
3481 tttctgctga agttgtcgac cttcgtacag taagcccact tgatatcgat acaattatcg
3541 cctctgtaga aaaaacagga cgcgcgattg tcgttcaaga ggcacaaaaa caagccggta
3601 ttgctgcaaa cgtagtagca gaaattaatg accgtgcgat cctgagcttg gaagcacctg
3661 tacttcgcgt tgcagcgcca gatacagtat ttcctttctc tcaagcggag agcgtatggc
3721 ttccaaacca taaagacgtt cttgaaacag caagaaaagt gcttgaattt taatcaaact
3781 gcataatcga gagggaagat gaacgttttc cctctattat atatctgttt tcaatgctta
3841 cgatgtaaac tttaaaattg cttaatcaaa ctaggaggtc gagaactgtg gcatttgaat
3901 ttaaacttcc agatatcggg gaaggtatcc acgaaggcga aatcgtaaaa tggtttgtca
3961 agcctaacga cgaggtagac gaagatgatg tactggctga agtccaaaat gataaagcag
4021 tagtagaaat tccttcacct gttaaaggaa aagtattaga attaaaagtt gaagagggaa
4081 cggttgcaac tgttggacaa acgattatta cgtttgatgc acctggttac gaagatcttc
4141 aatttaaagg cagcgacgag tctgacgatg cgaaaactga agcacaagtt cagtcaactg
4201 cagaagctgg acaagacgtt gcgaaagaag agcaagctca agagcctgca aaagctactg
4261 gcgcaggaca gcaggatcaa gctgaagttg acccgaacaa acgcgtgatc gctatgcctt
4321 ccgtacgtaa atatgcacgt gaaaaaggtg tagacatccg taaggttact ggttcaggca
4381 acaacggacg tgttgtaaaa gaagatatcg acagctttgt aaacggagga gcgcaagaag
4441 ctgcaccgca agaaacagct gcaccacaag aaacagctgc taaaccggct gctgcaccag
4501 ctccagaggg cgaattccca gaaacacgcg aaaaaatgag cggtatccgt aaagcaattg
4561 caaaagcgat ggttaactct aaacacactg ctcctcacgt aacgttaatg gacgaagtgg
4621 acgtaacaaa ccttgttgca catcgtaaac agttcaaaca ggttgctgct gatcaaggaa
4681 tcaagctgac ttacttgcct tacgttgtaa aagctcttac atctgcactg aaaaaattcc
4741 ctgttttaaa cacgtcaatt gacgataaaa cagatgaagt catccaaaaa cattacttca
4801 acatcggtat cgctgctgac actgaaaaag gcttgcttgt accggttgtg aaaaatgcag
4861 atcgtaaatc tgtctttgaa atttctgatg aaatcaatgg ccttgcaaca aaagctcgtg
4921 aaggcaagct tgctccagct gaaatgaaag gcgcatcttg cacaattaca aacatcggtt
4981 ctgccggcgg acaatggttc actccggtta tcaaccatcc agaagttgcg attcttggta
5041 tcggacgcat tgcagaaaaa gcgattgttc gtgatggcga aatcgtagca gctccagtct
5101 tagctctttc tctcagcttc gaccaccgta tgatcgacgg agcaactgcg caaaatgcat
5161 taaatcacat caagcgttta ctgaacgatc cacaattaat tttaatggag gcgtaatgtt
5221 atggtagtag gagatttccc tattgaaaca gatactcttg taattggtgc gggacctggc
5281 ggctatgtag ctgccatccg cgctgcacag cttggacaaa aagtaacagt cgttgaaaaa
5341 gcaactcttg gaggcgtttg tctgaacgtt ggatgtatcc cttcaaaagc gctgatcaat
5401 gcaggtcacc gttatgagaa tgcaaaacat tctgatgaca tgggaatcac tgctgagaat
5461 gtaacagttg atttcacaaa agttcaagaa tggaaagctt ctgttgtcaa caagcttact
5521 ggcggtgtag caggtcttct taaaggcaac aaagtagatg ttgtaaaagg tgaagcttac
5581 tttgtagaca gcaattcagt tcgtgttatg gatgagaact ctgctcaaac atacacgttt
5641 aaaaacgcaa tcattgctac tggttctcgt cctatcgaat tgccaaactt caaatatagt
5701 gagcgtgtcc tgaattcaac tggcgctttg gctcttaaag aaattcctaa aaagctcgtt
5761 gttatcggcg gcggatacat cggaactgaa cttggaactg cgtatgctaa cttcggtact
5821 gaacttgtta ttcttgaagg cggagatgaa attcttcctg gcttcgaaaa acaaatgagt
5881 tctctcgtta cacgcagact gaagaaaaaa ggcaacgttg aaatccatac aaacgcgatg
5941 gctaaaggcg ttgaagaaag accagacggc gtaacagtta ctttcgaagt aaaaggcgaa
6001 gaaaaaactg ttgatgctga ttacgtattg attacagtag gacgccgtcc aaacactgat
6061 gagcttggtc ttgagcaagt cggtatcgaa atgacggacc gcggtatcgt gaaaactgac
6121 aaacagtgcc gcacaaacgt acctaacatt tatgcaatcg gtgatatcat cgaaggaccg
6181 ccgcttgctc ataaagcatc ttacgaaggt aaaatcgctg cagaagctat cgctggagag
6241 cctgcagaaa tcgattacct tggtattcct gcggttgttt tctctgagcc tgaacttgca
6301 tcagttggtt acactgaagc acaggcgaaa gaagaaggtc ttgacattgt tgctgctaaa
6361 ttcccatttg cagcaaacgg ccgcgcgctt tctcttaacg aaacagacgg cttcatgaag
6421 ctgatcactc gtaaagagga cggtcttgtg atcggtgcgc aaatcgccgg agcaagtgct
6481 tctgatatga tttctgaatt aagcttagcg attgaaggcg gcatgactgc tgaagatatc
6541 gcaatgacaa ttcacgctca cccaacattg ggcgaaatca caatggaagc tgctgaagtg
6601 gcaatcggaa gtccgattca catcgtaaaa taattttcat atcaaaaaca gccccgcttt
6661 gagcgagggc tgttttttta ttttgacagc acatgctgta atggtttaat gatgtcttct
6721 ttctttttca cacatccctc gattttcacc acgacatggc gctgatcaac aaccataagg
6781 gtagggacag tttcaatctc atcttcccag ccttcttttt tatcatagac cttcatttta
6841 tctatttgct cgggataatc tttttctaaa tcaagcagtg catcgtaata aggcgcttcc
6901 tggtctattt ggttctcatc agaaaaaaac aggatttgtg tatgatgaat gttgtcaatc
6961 cggctttttt tcatcggatt gatggtggaa agcgtacagc ccgataaagc aaatactatt
7021 atcagcatgg ggaataccgc gcgatatcgc atctatcttc tcctctgtat aaacagtagt
7081 taactagctt tagtttatca cgagagacgg cttaatctgc ttcttgttac ctaattgtta
7141 tataattgaa accgttttta aagtgagatt acatattata tcaatcgttt aaaatagcaa
7201 cctggggtat attgaatttt ttgctgcatt cttggatgaa agttcaagaa aacgtgcaat
7261 tacttatgtg ctgtggcata tatatcgagt aaagcttctg tatgaagaag atattgtatg
7321 atggaaaatg gaaaaacaag gtgaatatga tgatatgaaa taaaatgtgt tatacagttt
7381 gttgttgaca ttttaaatgt gacatattaa tataataaca acaaaagaaa ttaagcgttt
7441 tcaaatttca aagaaatggg gaataagaga tgggtacgat tgtttgccaa gattgcaacg
7501 aagccattca ttactttgaa gatgagaaag tgacaacatt atatggaact tgctgcggac
7561 aatgtcaatg tcctgttgat gaagagtaaa aaaaagcatg cggcttaaag ccgcatggct
7621 ttttattttt tattgaattg ctttttgttc tttgatgacc cggatcatat gtaaagtcga
7681 gtcctcaggt ccttgaacag ggaggcctgc gtcaaggttt ttaaaaatgt agctgatgtt
7741 ttcttctgta ataatttctc ctgggatgaa gattggaata ccaggcggat ataccatgac
7801 aaattcagca ataatccgtc cggatgcttc ttttaatggg atgacttccg tatttgcata
7861 gaaagcatca cgcggagtca ttgccaataa aggtatttct ggaagcagaa cctcagtttg
7921 ctgatgtgtt acatcttgtt ctgacatttg ctgtgcgatc tcagttaaag cctctacaag
7981 ccggtctgca tcattttggc tgtcaccagg agtgaaaata cacaaaatat tgtacagatc
8041 agaaagctcc acctcaatat taaaggattc acgaagccat ttttccacgt catgtcctgt
8101 caggccaaga cttttcacag agataatcaa ttttgtcgga tcatagctgt aagctgcttt
8161 cgacccaaga atctcagaac cgacacaata aatgccttca atctgattga ggcgatctct
8221 cgtctgattg gcaagcttga gtgtttcctc agcaagctgg cggccttctg ttgcaaggcg
8281 ttttctggca acatccaaag aagcgagaag caagtaagaa gttgacgtag ttgtcagcat
8341 gctcaggatg gattgcactc tgtctttaga tacaaggcct tctctcatgt ttaaaataga
8401 gctttgtgtc agtgatccgc ccagcttgtg tacacttgtc gctgctatgt ccgctcctgc
8461 ctgcatagcc gatagcggca attcgtcgtg aaaatgaatg tgaacgccgt gtgcttcatc
8521 cacaagcact ggcacatcaa acgaatgagc gagttctacg atgcttttta agtcggctgc
8581 aacaccgaaa tatgtcgggt tgatgacgag aagccctttt gcatccggat gctcggtaag
8641 cgcccgttta gcagattcaa gtgtaattcc gtgggagatg cccaattcat tgtcaatttc
8701 aggatgaata aaaattggaa ccgctccgga aaatacaata gctgtcataa ttgatttatg
8761 aacatttctt ggaataataa ttttatcacc aggtccgcaa acagccatta ccattgtcat
8821 aatggcgccg cttgttcctt gaacagaaaa aaatgtatga tccgctccaa atgcttcagc
8881 agccagatct tgtgcttgtt taataatgcc ttttggcgcg tgcagatcgt ctaatggttc
8941 aatattaatc aaatctatgc ttaacgcgtt ttcaccgata aattgtctga attctgggtc
9001 cattcctgcc cctttttta
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS BSUPYDHY 9019 bp ds-DNA BCT 31-JAN-1991
DEFINITION B.subtilis pyruvate dehydrogenase complex genes, complete cds.
ACCESSION M57435 M31542
KEYWORDS dihydrolipoamide acetyltransferase E2 subunit;
dihydrolipoamide dehydrogenase E3 subunit;
pyruvate decarboxylase E1-alpha subunit;
pyruvate decarboxylase E1-beta subunit;
pyruvate dehydrogenase complex.
SOURCE B.subtilis (strain 168) BRB1 (sacA321 metB5) cell line DNA, clones
sc2 and sc4.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 9019)
AUTHORS Hemila,H., Palva,A., Paulin,L., Arvidson,S. and Palva,I.
TITLE Secretory S complex of Bacillus subtilis: Sequence analysis and
identity to pyruvate dehydrogenase
JOURNAL J. Bacteriol. 172, 5052-5063 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 540..1253
/label=ORF5
/codon_start=540
-35_signal 1548..1553
/gene="aceA"
-10_signal 1573..1578
/gene="aceA"
repeat_unit 1598..1611
/rpt_type=inverted
/gene="aceA"
RBS 1655..1666
/gene="aceA"
CDS 1677..2792
/EC_number="1.2.4.1"
/product="pyruvate decarboxylase E-1 alpha subunit"
/gene="aceA"
/codon_start=1677
RBS 2778..2787
/gene="aceA"
CDS 2796..3773
/EC_number="1.2.4.1"
/product="pyruvate decarboxylase E1-beta subunit"
/gene="aceA"
/codon_start=2796
-35_signal 3742..3747
/gene="E2 subunit gene"
-10_signal 3769..3774
/gene="E2 subunit gene"
repeat_unit 3790..3815
/rpt_type=inverted
/gene="E2 subunit gene"
RBS 3873..3879
/gene="E2 subunit gene"
CDS 3888..5216
/EC_number="2.3.1.12"
/product="dihydrolipoamide acetyltransferase E2 subunit"
/codon_start=3888
-35_signal 5134..5139
/gene="citL"
-10_signal 5160..5165
/gene="citL"
RBS 5204..5213
/gene="citL"
CDS 5221..6633
/EC_number="1.8.1.4"
/product="dihydrolipoamide dehydrogenase E3 subunit"
/gene="citL"
/codon_start=5221
CDS complement(7630..>9018)
/label=ORF6
/codon_start=9018
BASE COUNT 2796 a 1795 c 1982 g 2446 t
ORIGIN
1 gctgctgttt ttctgcatca gttggaggca gctcaacagg ttcggcagtc tctctgaggg
61 ctggatggcc gtcacgtacg atgttttcca tagtaatcaa gaaacttcac tcctaaagct
121 taatatgtaa attatatcgg atttcttcac aaaggtgcaa cgaaacgaat gaccagccgt
181 atatggctgg tcaatttcat gctgtgtggt tagcaacagc ctccacctaa ataagcagct
241 ccgacaatga ttaacagaat gaataaaaca acgattagcg caaaggtgcg gccgtagcca
301 tatccgcagc caccatatcc gtaaccacca taactgcagc agcctccgcc gtaaccatat
361 ccatacatat tacataacct cctcttttaa ttacgccaga gcctttataa gtaggcatag
421 cgtctaatat aacctatgta aaaacaaaaa agttgtatgg tcatctgtcc tgatttttca
481 cttttttgat ttaatttcag tattgcattt atacagcacg gctattgcca aatgtcccca
541 tggattctat agtagaaatt gtcaagattg ggggatttaa cgtgaaagac gctataaaac
601 ggcggcctgg aatcaagatc ggagcttttt gtgccatggc actgatatta acaggatgca
661 tgggacagga cccggttgat tcacttcatc attcattaga aaaagccgcg gaatcggaaa
721 agccgtttca agaagaacaa aacacactca aagaattaga ggccaaagaa aatcagcttt
781 acgatagtgt tatgaagctc aatatggatg attataaaaa aattgttgct ttatctaata
841 aggcgcttga aaatgtcagc aagcacgaag aacacctaaa gcttgaaaat gacagcatga
901 aaaaatcgga aacagaattt gaggaagcaa aaacgtcagc ggaacatatt aaggaaaaag
961 acataaaaga aaaggccgat gccgcagcaa accatatgga gaaaagatat acatcatatg
1021 gcactatgta taaagagtat aaaaaagcgc ttgagcttaa taaaaaactg tatatgcagc
1081 tgaaagacaa agatttgaca agagatgatc tggatcagca gatcgataaa gtcaatgctt
1141 cttatgaaaa gatccttaaa tatagcggag aatttaatga acaaaccgaa aaatataata
1201 aagcccggga agatttatat gatgctgcag gctatcgtgt gaaaaagagc tgactcgatc
1261 agctcttttt ttgaatgaaa tgcgattcgg atttcgttca aaattttcat ttctattgta
1321 gtacagcata gtaaggtata ctagtacagt tttgggtgcg gttggcatct gtttttttga
1381 tgaaaatgta aagcgaatac atttattgaa aatcagcgat gaaaacgatt gacggttctt
1441 ctttatttgg tgtaaactaa tccatgttaa gcggtgttgt attagttttt tacaacttcg
1501 aaataataca gttcaaagca gagtgtaact atcctacata aaaggttttg gcaaactggg
1561 gatcggctaa aatataatac gacttactgc tgatacttta gggtacccta agtttgtatg
1621 tataaaagga atgcacgtct aatgactgtt taaaagaaag gaagaggtga cttagtatgg
1681 ctgcaaaaac gaaaaaagct atcgttgaca gtaagaagca atttgatgcc attaaaaagc
1741 agttcgaaac gttccaaatt ttaaatgaaa agggagaagt cgtgaatgaa gcggcgatgc
1801 ctgatttaac tgatgatcaa ttaaaagagc taatgcgccg catggtattt acgcgtgtgc
1861 ttgaccaacg ctctatctca ttaaaccgtc aaggacgtct cggattttac gctcctactg
1921 cgggtcaaga agcttctcag attgcaacgc atttcgcgct tgaaaaagaa gactttgttc
1981 ttcctggata ccgtgatgtg cctcagttaa tttggcacgg ccttccatta tatcaagcgt
2041 ttcttttctc tcgcggacat ttcagaggaa accaaatgcc tgatgatgtg aatgcccttt
2101 ctccacaaat cattatcggt gctcaataca ttcaaactgc cggtgttgcg ctaggtctta
2161 aaaaacgcgg taagaaagct gtcgcaatca cttacactgg tgacggcgga cgttctcaag
2221 gggacttcta cgaaggaatt aactttgccg gagcttataa agcacctgca atcttcgtgg
2281 tgcaaaacaa ccgttacgcg atttcaactc ctgttgaaaa acaatctgca gctgaaacaa
2341 ttgcacaaaa agctgtagct gccggtattg tcggtgtaca agtagacgga atggatccgc
2401 ttgctgtata cgcagcaact gctgaagcac gcgagcgcgc aatcaacggc gaaggtccaa
2461 cactaattga aacacttaca ttccgttatg gcccgcacac aatggctggt gacgatccta
2521 ctaaatatcg tacaaaagaa atcgaaaatg agtgggaaca aaaagatccg cttgtacgtt
2581 tccgtgcgtt ccttgaaaac aaaggcttat ggtctgaaga agaagaagca aaagtgattg
2641 aggatgcgaa agaagaaatt aagcaagcga tcaaaaaagc tgatgctgaa ccgaagcaaa
2701 aagtaactga tttaatgaaa atcatgtacg aaaaaatgcc tcacaacctt gaggagcaat
2761 ttgaaattta tacacagaag gagtcgaagt aagccatggc gcaaatgaca atgattcaag
2821 caatcacgga tgcgttacgc acagaactga aaaatgacga aaatgtctta gttttcggag
2881 aagacgttgg tgtaaacggc ggcgtattcc gtgcgacaga aggattgcaa aaagaattcg
2941 gtgaagaccg tgtgtttgac acgccacttg ctgaatctgg tatcggcggt cttgcccttg
3001 gtttaggctt aaacggcttc cgtccggtaa tggaaatcca attcttcgga tttgtttatg
3061 aagtaatgga ttcagtttct ggccaaatgg ctcgtatgcg ctaccgttct ggcggacgct
3121 ggacttcacc tgtaacaatt cgttctccat tcggcggcgg tgttcatact cctgaacttc
3181 acgctgacag cttggaaggt cttgttgcac aacagcctgg tatcaaagtt gttattccat
3241 caactcctta cgatgccaaa ggacttttaa tttctgcgat cagagacaat gatcctgttg
3301 tcttcttaga gcatatgaag ctttaccgtt ctttccgtca ggaagttcct gaagaagaat
3361 acacaattga gcttggaaaa gctgacgtga aacgtgaagg tactgatctt tcaatcatca
3421 cttacggcgc aatggttcat gaatcattaa aagctgctga tgagcttgaa aaagacggca
3481 tttctgctga agttgtcgac cttcgtacag taagcccact tgatatcgat acaattatcg
3541 cctctgtaga aaaaacagga cgcgcgattg tcgttcaaga ggcacaaaaa caagccggta
3601 ttgctgcaaa cgtagtagca gaaattaatg accgtgcgat cctgagcttg gaagcacctg
3661 tacttcgcgt tgcagcgcca gatacagtat ttcctttctc tcaagcggag agcgtatggc
3721 ttccaaacca taaagacgtt cttgaaacag caagaaaagt gcttgaattt taatcaaact
3781 gcataatcga gagggaagat gaacgttttc cctctattat atatctgttt tcaatgctta
3841 cgatgtaaac tttaaaattg cttaatcaaa ctaggaggtc gagaactgtg gcatttgaat
3901 ttaaacttcc agatatcggg gaaggtatcc acgaaggcga aatcgtaaaa tggtttgtca
3961 agcctaacga cgaggtagac gaagatgatg tactggctga agtccaaaat gataaagcag
4021 tagtagaaat tccttcacct gttaaaggaa aagtattaga attaaaagtt gaagagggaa
4081 cggttgcaac tgttggacaa acgattatta cgtttgatgc acctggttac gaagatcttc
4141 aatttaaagg cagcgacgag tctgacgatg cgaaaactga agcacaagtt cagtcaactg
4201 cagaagctgg acaagacgtt gcgaaagaag agcaagctca agagcctgca aaagctactg
4261 gcgcaggaca gcaggatcaa gctgaagttg acccgaacaa acgcgtgatc gctatgcctt
4321 ccgtacgtaa atatgcacgt gaaaaaggtg tagacatccg taaggttact ggttcaggca
4381 acaacggacg tgttgtaaaa gaagatatcg acagctttgt aaacggagga gcgcaagaag
4441 ctgcaccgca agaaacagct gcaccacaag aaacagctgc taaaccggct gctgcaccag
4501 ctccagaggg cgaattccca gaaacacgcg aaaaaatgag cggtatccgt aaagcaattg
4561 caaaagcgat ggttaactct aaacacactg ctcctcacgt aacgttaatg gacgaagtgg
4621 acgtaacaaa ccttgttgca catcgtaaac agttcaaaca ggttgctgct gatcaaggaa
4681 tcaagctgac ttacttgcct tacgttgtaa aagctcttac atctgcactg aaaaaattcc
4741 ctgttttaaa cacgtcaatt gacgataaaa cagatgaagt catccaaaaa cattacttca
4801 acatcggtat cgctgctgac actgaaaaag gcttgcttgt accggttgtg aaaaatgcag
4861 atcgtaaatc tgtctttgaa atttctgatg aaatcaatgg ccttgcaaca aaagctcgtg
4921 aaggcaagct tgctccagct gaaatgaaag gcgcatcttg cacaattaca aacatcggtt
4981 ctgccggcgg acaatggttc actccggtta tcaaccatcc agaagttgcg attcttggta
5041 tcggacgcat tgcagaaaaa gcgattgttc gtgatggcga aatcgtagca gctccagtct
5101 tagctctttc tctcagcttc gaccaccgta tgatcgacgg agcaactgcg caaaatgcat
5161 taaatcacat caagcgttta ctgaacgatc cacaattaat tttaatggag gcgtaatgtt
5221 atggtagtag gagatttccc tattgaaaca gatactcttg taattggtgc gggacctggc
5281 ggctatgtag ctgccatccg cgctgcacag cttggacaaa aagtaacagt cgttgaaaaa
5341 gcaactcttg gaggcgtttg tctgaacgtt ggatgtatcc cttcaaaagc gctgatcaat
5401 gcaggtcacc gttatgagaa tgcaaaacat tctgatgaca tgggaatcac tgctgagaat
5461 gtaacagttg atttcacaaa agttcaagaa tggaaagctt ctgttgtcaa caagcttact
5521 ggcggtgtag caggtcttct taaaggcaac aaagtagatg ttgtaaaagg tgaagcttac
5581 tttgtagaca gcaattcagt tcgtgttatg gatgagaact ctgctcaaac atacacgttt
5641 aaaaacgcaa tcattgctac tggttctcgt cctatcgaat tgccaaactt caaatatagt
5701 gagcgtgtcc tgaattcaac tggcgctttg gctcttaaag aaattcctaa aaagctcgtt
5761 gttatcggcg gcggatacat cggaactgaa cttggaactg cgtatgctaa cttcggtact
5821 gaacttgtta ttcttgaagg cggagatgaa attcttcctg gcttcgaaaa acaaatgagt
5881 tctctcgtta cacgcagact gaagaaaaaa ggcaacgttg aaatccatac aaacgcgatg
5941 gctaaaggcg ttgaagaaag accagacggc gtaacagtta ctttcgaagt aaaaggcgaa
6001 gaaaaaactg ttgatgctga ttacgtattg attacagtag gacgccgtcc aaacactgat
6061 gagcttggtc ttgagcaagt cggtatcgaa atgacggacc gcggtatcgt gaaaactgac
6121 aaacagtgcc gcacaaacgt acctaacatt tatgcaatcg gtgatatcat cgaaggaccg
6181 ccgcttgctc ataaagcatc ttacgaaggt aaaatcgctg cagaagctat cgctggagag
6241 cctgcagaaa tcgattacct tggtattcct gcggttgttt tctctgagcc tgaacttgca
6301 tcagttggtt acactgaagc acaggcgaaa gaagaaggtc ttgacattgt tgctgctaaa
6361 ttcccatttg cagcaaacgg ccgcgcgctt tctcttaacg aaacagacgg cttcatgaag
6421 ctgatcactc gtaaagagga cggtcttgtg atcggtgcgc aaatcgccgg agcaagtgct
6481 tctgatatga tttctgaatt aagcttagcg attgaaggcg gcatgactgc tgaagatatc
6541 gcaatgacaa ttcacgctca cccaacattg ggcgaaatca caatggaagc tgctgaagtg
6601 gcaatcggaa gtccgattca catcgtaaaa taattttcat atcaaaaaca gccccgcttt
6661 gagcgagggc tgttttttta ttttgacagc acatgctgta atggtttaat gatgtcttct
6721 ttctttttca cacatccctc gattttcacc acgacatggc gctgatcaac aaccataagg
6781 gtagggacag tttcaatctc atcttcccag ccttcttttt tatcatagac cttcatttta
6841 tctatttgct cgggataatc tttttctaaa tcaagcagtg catcgtaata aggcgcttcc
6901 tggtctattt ggttctcatc agaaaaaaac aggatttgtg tatgatgaat gttgtcaatc
6961 cggctttttt tcatcggatt gatggtggaa agcgtacagc ccgataaagc aaatactatt
7021 atcagcatgg ggaataccgc gcgatatcgc atctatcttc tcctctgtat aaacagtagt
7081 taactagctt tagtttatca cgagagacgg cttaatctgc ttcttgttac ctaattgtta
7141 tataattgaa accgttttta aagtgagatt acatattata tcaatcgttt aaaatagcaa
7201 cctggggtat attgaatttt ttgctgcatt cttggatgaa agttcaagaa aacgtgcaat
7261 tacttatgtg ctgtggcata tatatcgagt aaagcttctg tatgaagaag atattgtatg
7321 atggaaaatg gaaaaacaag gtgaatatga tgatatgaaa taaaatgtgt tatacagttt
7381 gttgttgaca ttttaaatgt gacatattaa tataataaca acaaaagaaa ttaagcgttt
7441 tcaaatttca aagaaatggg gaataagaga tgggtacgat tgtttgccaa gattgcaacg
7501 aagccattca ttactttgaa gatgagaaag tgacaacatt atatggaact tgctgcggac
7561 aatgtcaatg tcctgttgat gaagagtaaa aaaaagcatg cggcttaaag ccgcatggct
7621 ttttattttt tattgaattg ctttttgttc tttgatgacc cggatcatat gtaaagtcga
7681 gtcctcaggt ccttgaacag ggaggcctgc gtcaaggttt ttaaaaatgt agctgatgtt
7741 ttcttctgta ataatttctc ctgggatgaa gattggaata ccaggcggat ataccatgac
7801 aaattcagca ataatccgtc cggatgcttc ttttaatggg atgacttccg tatttgcata
7861 gaaagcatca cgcggagtca ttgccaataa aggtatttct ggaagcagaa cctcagtttg
7921 ctgatgtgtt acatcttgtt ctgacatttg ctgtgcgatc tcagttaaag cctctacaag
7981 ccggtctgca tcattttggc tgtcaccagg agtgaaaata cacaaaatat tgtacagatc
8041 agaaagctcc acctcaatat taaaggattc acgaagccat ttttccacgt catgtcctgt
8101 caggccaaga cttttcacag agataatcaa ttttgtcgga tcatagctgt aagctgcttt
8161 cgacccaaga atctcagaac cgacacaata aatgccttca atctgattga ggcgatctct
8221 cgtctgattg gcaagcttga gtgtttcctc agcaagctgg cggccttctg ttgcaaggcg
8281 ttttctggca acatccaaag aagcgagaag caagtaagaa gttgacgtag ttgtcagcat
8341 gctcaggatg gattgcactc tgtctttaga tacaaggcct tctctcatgt ttaaaataga
8401 gctttgtgtc agtgatccgc ccagcttgtg tacacttgtc gctgctatgt ccgctcctgc
8461 ctgcatagcc gatagcggca attcgtcgtg aaaatgaatg tgaacgccgt gtgcttcatc
8521 cacaagcact ggcacatcaa acgaatgagc gagttctacg atgcttttta agtcggctgc
8581 aacaccgaaa tatgtcgggt tgatgacgag aagccctttt gcatccggat gctcggtaag
8641 cgcccgttta gcagattcaa gtgtaattcc gtgggagatg cccaattcat tgtcaatttc
8701 aggatgaata aaaattggaa ccgctccgga aaatacaata gctgtcataa ttgatttatg
8761 aacatttctt ggaataataa ttttatcacc aggtccgcaa acagccatta ccattgtcat
8821 aatggcgccg cttgttcctt gaacagaaaa aaatgtatga tccgctccaa atgcttcagc
8881 agccagatct tgtgcttgtt taataatgcc ttttggcgcg tgcagatcgt ctaatggttc
8941 aatattaatc aaatctatgc ttaacgcgtt ttcaccgata aattgtctga attctgggtc
9001 cattcctgcc cctttttta
//