GenBank-Updates@genbank.bio.net (12/01/90)
LOCUS CLOSSPC2 544 bp ds-DNA BCT 01-DEC-1990
DEFINITION Clostridium perfringens acid-soluble spore protein C2 gene,
complete cds.
ACCESSION M57434 M31619
KEYWORDS acid-soluble spore protein; spore nucleoid.
SOURCE C. perfringens DNA, clone pUAH735.
ORGANISM Clostridium perfringens
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 544)
AUTHORS Holck,A., Blom,H. and Granum,P.
TITLE Cloning and sequencing of the genes encoding acid-soluble spore
proteins from Clostridium perfringens
JOURNAL Gene 91, 107-111 (1990)
STANDARD full staff_review
FEATURES from to/span description
rpt 95 109 direct repeat
signal 122 127 -35 region
rpt 129 143 repeat
signal 146 152 -10 region
binding 206 212 ribosome binding site
pept 220 402 acid-soluble spore protein C2
BASE COUNT 221 a 68 c 69 g 186 t
ORIGIN
1 aataattttg tttgagcttt cttcaaaaat atatttaatt ttactttcat aagataaatc
61 aatttcactt gtaattttat accataatgt gatttttaat cctatattgg atataagttt
121 atagattatt aaatccaatt ttgcacatca taatattgaa aggaaaagaa aaacttaacc
181 tttcaaaaaa tcttattaaa cttaaaggag gaattcaaaa tgtcacaaca tttagttcca
241 gaagcaaaaa acggattatc aaaattcaaa aatgaggtag caaatgaaat gggagtacca
301 ttctcagatt ataatggaga ccttagctca agacaatgcg gaagtgttgg tggagaaatg
361 gttaaaagaa tggtagaaaa atacgaacaa tcaatgaaat aataacaaaa ataagaggta
421 agaaaattct tacctcttat ttttaattta agaaactttt ttaagcataa agtttctcca
481 atttccatct tgtattaata tactgttatt ttcaaagtca tctttatcat tcattccata
541 ataa
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS CLOSSPC2 544 bp ds-DNA BCT 31-JAN-1991
DEFINITION Clostridium perfringens acid-soluble spore protein C2 gene,
complete cds.
ACCESSION M57434 M31619
KEYWORDS acid-soluble spore protein; spore nucleoid.
SOURCE C. perfringens DNA, clone pUAH735.
ORGANISM Clostridium perfringens
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 544)
AUTHORS Holck,A., Blom,H. and Granum,P.
TITLE Cloning and sequencing of the genes encoding acid-soluble spore
proteins from Clostridium perfringens
JOURNAL Gene 91, 107-111 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
repeat_region 95..109
/rpt_type=direct
/gene="sspC2"
/evidence=EXPERIMENTAL
-35_signal 122..127
/gene="sspC2"
repeat_region 129..143
/gene="sspC2"
/evidence=EXPERIMENTAL
-10_signal 146..152
/gene="sspC2"
RBS 206..212
/gene="sspC2"
CDS 220..402
/product="acid-soluble spore protein C2"
/gene="sspC2"
/evidence=EXPERIMENTAL
/codon_start=220
BASE COUNT 221 a 68 c 69 g 186 t
ORIGIN
1 aataattttg tttgagcttt cttcaaaaat atatttaatt ttactttcat aagataaatc
61 aatttcactt gtaattttat accataatgt gatttttaat cctatattgg atataagttt
121 atagattatt aaatccaatt ttgcacatca taatattgaa aggaaaagaa aaacttaacc
181 tttcaaaaaa tcttattaaa cttaaaggag gaattcaaaa tgtcacaaca tttagttcca
241 gaagcaaaaa acggattatc aaaattcaaa aatgaggtag caaatgaaat gggagtacca
301 ttctcagatt ataatggaga ccttagctca agacaatgcg gaagtgttgg tggagaaatg
361 gttaaaagaa tggtagaaaa atacgaacaa tcaatgaaat aataacaaaa ataagaggta
421 agaaaattct tacctcttat ttttaattta agaaactttt ttaagcataa agtttctcca
481 atttccatct tgtattaata tactgttatt ttcaaagtca tctttatcat tcattccata
541 ataa
//