GenBank-Updates@genbank.bio.net (11/27/90)
LOCUS DOGSNCE 3199 bp ss-mRNA MAM 27-NOV-1990
DEFINITION Dog cardiac sarcolemmal sodium-calcium exchanger mRNA, complete
cds.
ACCESSION M57523 M36119
KEYWORDS sodium-calcium exchanger.
SOURCE Dog cardiac muscle, cDNA to mRNA, clone TB11 and A4.
ORGANISM Canis sp.
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Carnivora; Caniformia; Canoidea; Canidae.
REFERENCE 1 (bases 1 to 3199)
AUTHORS Nicoll,D.A., Longoni,S. and Philipson,K.D.
TITLE Molecular cloning and functional expression of the cardiac
sarcolemmal Na+-Ca2+ exchanger
JOURNAL Science 250, 562-565 (1990)
STANDARD full staff_entry
FEATURES from to/span description
pept 28 2940 sodium-calcium exchanger
binding 251 270 calmodulin binding site
binding 680 694 peptide antigen
BASE COUNT 847 a 697 c 828 g 827 t
ORIGIN
1 acacttggag gtctaccatt gtacaacatg ctgcagttaa gactattacc taccttttca
61 atgggatgtc atctgttagc cgttgtggct ctcttatttt cccacgtgga ccttataagt
121 gcagagacag aaatggaagg agaaggcaat gaaaccggcg agtgtactgg ctcatattat
181 tgtaagaagg gggtgatttt acccatttgg gagccccaag acccttcctt tggagacaaa
241 attgccagag ctactgtgta ttttgtggcc atggtctaca tgtttctcgg agtctctatc
301 attgctgacc ggttcatgtc ctctatagaa gtcatcacat ctcaagaaaa agaaataacc
361 ataaagaaac ccaatggaga gaccaccaag accactgtga ggatctggaa tgagactgtt
421 tccaatctaa ccttgatggc cctgggatct tctgctccag agattctcct ttcagtaatt
481 gaagtatgtg gccataactt cactgcagga gacctaggtc ccagcaccat tgtgggaagt
541 gctgcattca acatgttcat catcatcgcg ctttgtgttt atgtggtccc agatggagag
601 acaaggaaga ttaagcattt gcgtgtgttc ttcgtgacag cagcctggag catctttgcc
661 tatacgtggc tttacattat tttgtctgtc atctctcctg gggtcgtgga agtctgggaa
721 ggtttgctta ctttcttctt ctttcctatc tgtgttgtgt tcgcttgggt ggcagatagg
781 aggctgctgt tttacaagta tgtctacaag aggtatcggg ccggcaagca gaggggaatg
841 atcatcgagc acgaaggaga caggccatct tccaagactg aaattgaaat ggatgggaaa
901 gtggtcaatt cccacgttga caatttctta gatggtgctc tggttctgga ggttgatgag
961 agggaccaag atgatgaaga ggcgaggcga gaaatggcta ggattctgaa ggagctcaag
1021 cagaagcatc cggagaaaga aatagagcaa ttgattgaat tagctaacta ccaagtccta
1081 agtcagcagc agaaaagccg agcattttac cgcatccaag ctactcgcct gatgactgga
1141 gctggcaaca ttttaaagag gcacgcagct gaccaggcaa ggaaggctgt cagcatgcat
1201 gaggtcaaca ctgaagtggc tgaaaatgac cccgtgagta agatcttctt tgaacaaggg
1261 acctatcagt gtctggagaa ctgtgggact gtagccctga ccattatccg cagaggtggc
1321 gatttgacta acactgtgtt tgttgacttc agaacagagg atggcacggc caacgctggc
1381 tctgattatg aatttactga aggaactgtg gtctttaagc caggtgagac ccagaaggaa
1441 atcagagttg gcatcatcga tgatgacatc tttgaggagg atgagaattt ccttgtgcac
1501 ttgagcaatg ttaaagtatc ttctgaggcc tcagaagacg gcatcctgga agccaatcat
1561 gtttctgcgc tcgcttgcct gggatctccc tccaccgcca ccgtgacgat ttttgacgat
1621 gaccacgctg gcatctttac ttttgaggag cccgtgactc acgtgagtga gagcattggc
1681 attatggagg tgaaggtatt gcgaacatct ggagctcgag gaaatgttat cgttccctat
1741 aaaaccatcg aagggactgc cagaggtgga ggggaggact ttgaggacac ttgtggagag
1801 ctcgaattcc agaacgatga aattgtcaaa acaatatcag tcaaggtaat tgatgatgag
1861 gagtatgaga aaaacaagac cttcttcctt gagattggag agccccgcct ggtggagatg
1921 agtgagaaga aagccctgtt attgaatgag cttggtggct tcacaataac aggaaaatac
1981 ctgtatggcc aacctgtctt ccggaaagtt catgctagag aacatccgat tccctctact
2041 gtaatcacca ttgcagagga atatgatgac aagcaaccgc tgaccagcaa agaggaggag
2101 gagaggcgca ttgcagaaat ggggcggccc attctgggag aacacaccaa gctggaagtg
2161 atcattgaag aatcctatga attcaagagt accgtggaca aacttattaa gaagacaaac
2221 ttagcccttg tggttgggac aaacagctgg agagagcagt ttattgaagc catcaccgtc
2281 agtgctgggg aagatgatga tgacgatgaa tgtggagagg agaagctgcc ctcctgtttc
2341 gattatgtga tgcattttct gactgtgttc tggaaagtcc tcttcgcctt cgtccccccg
2401 acagaatact ggaatggctg ggcctgtttc attgtctcca tcctcatgat tggcatactg
2461 acagctttca ttggagacct ggcttcccac tttggatgca ccatcggctt gaaagattct
2521 gtgactgcgg tagtgttcgt cgcacttgga acttcggtac cagacacatt tgcaagcaaa
2581 gtggcagcca cccaggatca gtatgcagac gcgtccatag gtaacgtcac tggcagcaac
2641 gcggtgaacg tctttctggg aatcggcgtg gcctggtcca tcgctgccat ctaccatgca
2701 gccaatgggg aacagttcaa agtgtcccct ggcacgctag ctttctctgt cactctcttc
2761 accatttttg ctttcatcaa tgtgggggtg ctgctgtatc ggcggaggcc agagatcgga
2821 ggtgagctgg gtgggccccg gactgccaag ctcctcacat cctgcctctt cgtgctccta
2881 tggctcttgt acattttctt ctcctccctg gaggcctact gccacataaa aggcttctaa
2941 aggaacaatc agatatagta aatttatata tatatacgta tatatataca taaaatatta
3001 tgtataatga acagaggaaa ctgacatttg tcatgttcac ttacctgctg atggaatcca
3061 gcttcaagag catactctgt actagggctg aagtgagaaa ccatcacctc ccattcccag
3121 gggcgtcatc acattgaaca aggcatggag gcaggggcat ctttgcagct cagcctagaa
3181 ggactgtgtt ctggaattc
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS DOGSNCE 3199 bp ss-mRNA MAM 31-JAN-1991
DEFINITION Dog cardiac sarcolemmal sodium-calcium exchanger mRNA, complete
cds.
ACCESSION M57523 M36119
KEYWORDS sodium-calcium exchanger.
SOURCE Dog cardiac muscle, cDNA to mRNA, clone TB11 and A4.
ORGANISM Canis sp.
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Carnivora; Caniformia; Canoidea; Canidae.
REFERENCE 1 (bases 1 to 3199)
AUTHORS Nicoll,D.A., Longoni,S. and Philipson,K.D.
TITLE Molecular cloning and functional expression of the cardiac
sarcolemmal Na+-Ca2+ exchanger
JOURNAL Science 250, 562-565 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 28..2940
/product="sodium-calcium exchanger"
/codon_start=28
protein_bind 251..270
/note="calmodulin binding site"
protein_bind 680..694
/note="peptide antigen"
BASE COUNT 847 a 697 c 828 g 827 t
ORIGIN
1 acacttggag gtctaccatt gtacaacatg ctgcagttaa gactattacc taccttttca
61 atgggatgtc atctgttagc cgttgtggct ctcttatttt cccacgtgga ccttataagt
121 gcagagacag aaatggaagg agaaggcaat gaaaccggcg agtgtactgg ctcatattat
181 tgtaagaagg gggtgatttt acccatttgg gagccccaag acccttcctt tggagacaaa
241 attgccagag ctactgtgta ttttgtggcc atggtctaca tgtttctcgg agtctctatc
301 attgctgacc ggttcatgtc ctctatagaa gtcatcacat ctcaagaaaa agaaataacc
361 ataaagaaac ccaatggaga gaccaccaag accactgtga ggatctggaa tgagactgtt
421 tccaatctaa ccttgatggc cctgggatct tctgctccag agattctcct ttcagtaatt
481 gaagtatgtg gccataactt cactgcagga gacctaggtc ccagcaccat tgtgggaagt
541 gctgcattca acatgttcat catcatcgcg ctttgtgttt atgtggtccc agatggagag
601 acaaggaaga ttaagcattt gcgtgtgttc ttcgtgacag cagcctggag catctttgcc
661 tatacgtggc tttacattat tttgtctgtc atctctcctg gggtcgtgga agtctgggaa
721 ggtttgctta ctttcttctt ctttcctatc tgtgttgtgt tcgcttgggt ggcagatagg
781 aggctgctgt tttacaagta tgtctacaag aggtatcggg ccggcaagca gaggggaatg
841 atcatcgagc acgaaggaga caggccatct tccaagactg aaattgaaat ggatgggaaa
901 gtggtcaatt cccacgttga caatttctta gatggtgctc tggttctgga ggttgatgag
961 agggaccaag atgatgaaga ggcgaggcga gaaatggcta ggattctgaa ggagctcaag
1021 cagaagcatc cggagaaaga aatagagcaa ttgattgaat tagctaacta ccaagtccta
1081 agtcagcagc agaaaagccg agcattttac cgcatccaag ctactcgcct gatgactgga
1141 gctggcaaca ttttaaagag gcacgcagct gaccaggcaa ggaaggctgt cagcatgcat
1201 gaggtcaaca ctgaagtggc tgaaaatgac cccgtgagta agatcttctt tgaacaaggg
1261 acctatcagt gtctggagaa ctgtgggact gtagccctga ccattatccg cagaggtggc
1321 gatttgacta acactgtgtt tgttgacttc agaacagagg atggcacggc caacgctggc
1381 tctgattatg aatttactga aggaactgtg gtctttaagc caggtgagac ccagaaggaa
1441 atcagagttg gcatcatcga tgatgacatc tttgaggagg atgagaattt ccttgtgcac
1501 ttgagcaatg ttaaagtatc ttctgaggcc tcagaagacg gcatcctgga agccaatcat
1561 gtttctgcgc tcgcttgcct gggatctccc tccaccgcca ccgtgacgat ttttgacgat
1621 gaccacgctg gcatctttac ttttgaggag cccgtgactc acgtgagtga gagcattggc
1681 attatggagg tgaaggtatt gcgaacatct ggagctcgag gaaatgttat cgttccctat
1741 aaaaccatcg aagggactgc cagaggtgga ggggaggact ttgaggacac ttgtggagag
1801 ctcgaattcc agaacgatga aattgtcaaa acaatatcag tcaaggtaat tgatgatgag
1861 gagtatgaga aaaacaagac cttcttcctt gagattggag agccccgcct ggtggagatg
1921 agtgagaaga aagccctgtt attgaatgag cttggtggct tcacaataac aggaaaatac
1981 ctgtatggcc aacctgtctt ccggaaagtt catgctagag aacatccgat tccctctact
2041 gtaatcacca ttgcagagga atatgatgac aagcaaccgc tgaccagcaa agaggaggag
2101 gagaggcgca ttgcagaaat ggggcggccc attctgggag aacacaccaa gctggaagtg
2161 atcattgaag aatcctatga attcaagagt accgtggaca aacttattaa gaagacaaac
2221 ttagcccttg tggttgggac aaacagctgg agagagcagt ttattgaagc catcaccgtc
2281 agtgctgggg aagatgatga tgacgatgaa tgtggagagg agaagctgcc ctcctgtttc
2341 gattatgtga tgcattttct gactgtgttc tggaaagtcc tcttcgcctt cgtccccccg
2401 acagaatact ggaatggctg ggcctgtttc attgtctcca tcctcatgat tggcatactg
2461 acagctttca ttggagacct ggcttcccac tttggatgca ccatcggctt gaaagattct
2521 gtgactgcgg tagtgttcgt cgcacttgga acttcggtac cagacacatt tgcaagcaaa
2581 gtggcagcca cccaggatca gtatgcagac gcgtccatag gtaacgtcac tggcagcaac
2641 gcggtgaacg tctttctggg aatcggcgtg gcctggtcca tcgctgccat ctaccatgca
2701 gccaatgggg aacagttcaa agtgtcccct ggcacgctag ctttctctgt cactctcttc
2761 accatttttg ctttcatcaa tgtgggggtg ctgctgtatc ggcggaggcc agagatcgga
2821 ggtgagctgg gtgggccccg gactgccaag ctcctcacat cctgcctctt cgtgctccta
2881 tggctcttgt acattttctt ctcctccctg gaggcctact gccacataaa aggcttctaa
2941 aggaacaatc agatatagta aatttatata tatatacgta tatatataca taaaatatta
3001 tgtataatga acagaggaaa ctgacatttg tcatgttcac ttacctgctg atggaatcca
3061 gcttcaagag catactctgt actagggctg aagtgagaaa ccatcacctc ccattcccag
3121 gggcgtcatc acattgaaca aggcatggag gcaggggcat ctttgcagct cagcctagaa
3181 ggactgtgtt ctggaattc
//