GenBank-Updates@genbank.bio.net (12/08/90)
LOCUS DVUHYD 3100 bp ds-DNA BCT 08-DEC-1990
DEFINITION D.vulgaris [NiFe] hydrogenase large and small subunit genes,
complete cds.
ACCESSION M58339 M33174
KEYWORDS [NiFe] hydrogenase.
SOURCE D.vulgaris DNA.
ORGANISM Desulfovibrio vulgaris
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Sulfate- or sulfur-reducing dissimilatory bacteria.
REFERENCE 1 (bases 1 to 3100)
AUTHORS Deckers,H.M., Wilson,F.R. and Voordouw,G.
TITLE Cloning and sequencing of a [NiFe] hydrogenase operon from
Desulfovibrio vulgaris Miyazaki F
JOURNAL J. Gen. Microbiol. 136, 2021-2028 (1990)
STANDARD simple staff_entry
FEATURES from to/span description
signal 207 212 small subunit ribosome binding site
pept 219 1172 [NiFe] hydrogenase small subunit
precursor(hyd)
sigp 219 368 [NiFe] hydrogenase small subunit signal
peptide
matp 369 1160 [NiFe] hydrogenase small subunit
signal 1219 1224 large subunit ribosome binding site
pept 1231 2934 [NiFe] hydrogenase large subunit (hyd)
signal 2987 3013 inverted repeat
BASE COUNT 620 a 1090 c 927 g 463 t
ORIGIN
1 cgcgatgcgg aaatgtgtgc ctcatttaca ttttcaattg gaataacgtt tcaatgtgtt
61 gtaccttccc ggcaaacgtg cggacaggtc accgcgggaa acggatgcac gcagaagcgg
121 tcgcagccgg ggggcctttc gcaccagtac gacccttacc ccgccgcgcc gctggcggcg
181 ggcaggcacg gtgatacgcc gtgccgaagg aggcagggat gaaaatctcg atcggtctcg
241 gcaaggaggg cgtggaggaa aggcttgcgg aacgcggcgt gtctcgacgc gacttcctca
301 agttctgtac ggccatcgcc gtgaccatgg gcatgggccc cgcgttcgcg ccggaagttg
361 cccgcgcgct catgggtccc cggcgcccgt ccgtggtcta cctgcacaac gccgaatgca
421 ccggctgttc cgaatcggtg ctgcgcgcgt tcgaacccta catcgacacc ctgattctgg
481 acacgctgtc cctcgactac catgagacca tcatggccgc cgcgggcgat gcggcggaag
541 ccgccctgga gcaggccgtc aacagcccgc acggcttcat cgccgtggtg gaaggcggca
601 ttcccacggc tgccaacggc atctacggca aggtggccaa ccacaccatg ctggacatct
661 gcagccgcat cctgcccaag gcccaggccg tcatcgcgta cggcacctgc gccaccttcg
721 gcggcgtgca ggcagccaag cccaacccca ccggtgccaa gggcgtcaac gacgcgctga
781 agcaccttgg cgtcaaggcc atcaacatcg ccggttgccc gccgaacccg tacaacctgg
841 tcggcaccat cgtgtactac ctgaagaaca aggccgcgcc cgagctggac agcctgaacc
901 ggcccaccat gttcttcggc cagaccgtgc acgaacagtg cccccgccta ccgcacttcg
961 acgcgggcga attcgccccg tcgttcgaat cggaagaagc ccgcaagggc tggtgcctct
1021 acgagctggg ctgcaagggc ccggtgacca tgaacaactg cccgaagatc aagttcaacc
1081 agaccaactg gcccgtggac gcggggcacc cctgcatcgg gtgcagcgaa cccgatttct
1141 gggacgccat gaccccgttc taccagaact gatcacgcgc acgcgctagg tcacccaata
1201 cccccatagg caacagccaa ggagaatacc atgagcggct gcagagccca gaatgctccg
1261 ggcggcatcc ccgtgacgcc caagagctcc tatagcggtc ccatcgtcgt cgaccccgtt
1321 acccgcatcg aaggccacct gcgcatcgag gtggaagtgg agaacggcaa ggtcaagaac
1381 gcctacagca gttccacgct gttccggggc cttgaaatca tcctgaaggg ccgcgacccc
1441 cgcgacgccc agcacttcac ccagcgcacc tgcggcgtgt gcacctatac ccatgcgctg
1501 gcctccaccc gctgcgtgga caacgccgtg ggcgtgcaca ttcccaagaa cgccacctac
1561 atccgcaacc tggtgctggg cgcgcagtac ctgcacgacc acatcgtgca cttctaccac
1621 ctgcacgccc tggacttcgt ggacgtgacc gccgcgctga aggccgaccc ggccaaggcc
1681 gccaaggtgg cctcgtccat ctcgccccgc aagaccacgg cggcggacct gaaggcggtg
1741 caggacaagc tgaagacctt cgtggaaacc gggcagctcg gcccgttcac caatgcctac
1801 ttcctgggcg gccaccccgc ctactacctg gatccggaaa ccaacctcat cgccaccgcc
1861 cactacctgg aagccctgcg ccttcaggtg aaggccgcgc gcgccatggc cgttttcggc
1921 gccaagaacc cgcacaccca gttcaccgtg gtgggcggcg tgacctgcta cgacgccctg
1981 accccgcagc gcatcgccga attcgaggcg ctgtggaagg aaaccaaggc gttcgttgat
2041 gaagtgtaca tccccgacct gctggtggtt gccgcggcct acaaggattg gacgcagtac
2101 ggcggcaccg acaacttcat caccttcggc gaattcccga aggacgagta cgacctgaac
2161 agccggttct tcaagccggg cgtggtcttc aagcgcgact tcaagaacat caagccgttc
2221 gacaagatgc agatcgaaga acacgtgcgc cacagctggt acgaaggcgc ggaagcccgc
2281 cacccgtgga agggccagac ccagcccaag tacaccgacc tgcacggcga cgaccgctac
2341 tcgtggatga aggccccccg ctacatgggc gagcccatgg aaacggggcc gctggcccag
2401 gtgctgatcg cctactcgca gggccacccc aaggtgaagg ccgtgaccga cgcagtgctt
2461 gccaagctgg gcgtgggccc cgaggccctg ttctccaccc tgggccgcac ggcggcgcgc
2521 ggcatcgaaa ccgccgtcat cgcagaatac gtgggagtga tgctgcagga gtacaaggac
2581 aacatcgcca agggcgacaa cgtcatctgc gccccgtggg aaatgcccaa gcaggcggaa
2641 ggcgtgggct tcgtcaacgc cccgcgcggc ggcctgtcgc actggatccg catcgaggac
2701 ggcaagatcg gcaacttcca actggtcgtg ccctccacct ggacccttgg cccccgctgc
2761 gacaagaaca acgtgtcgcc ggtggaagcc tcgctcatcg gcaccccggt ggccgatgcc
2821 aagcgcccgg tggaaatcct gcgcacggtg cattcgttcg acccgtgcat cgcctgcggc
2881 gtgcacgtca tcgacgggca caccaacgaa gtgcacaagt tccgcatcct gtaagctgtc
2941 cgcagccccg cctgccaagg cggcgtgccg gaatgtcata tcgaccgaag gcccggcctt
3001 gtgccgggcc ctcggactat ctgagaagct ggcacagcgg cagccccgcc tgtgcaccgc
3061 ctaccggacg acggccccga aaactgcgcg tcaacggcgg
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS DVUHYD 3100 bp ds-DNA BCT 31-JAN-1991
DEFINITION D.vulgaris [NiFe] hydrogenase large and small subunit genes,
complete cds.
ACCESSION M58339 M33174
KEYWORDS [NiFe] hydrogenase.
SOURCE D.vulgaris DNA.
ORGANISM Desulfovibrio vulgaris
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Sulfate- or
sulfur-reducing dissimilatory bacteria.
REFERENCE 1 (bases 1 to 3100)
AUTHORS Deckers,H.M., Wilson,F.R. and Voordouw,G.
TITLE Cloning and sequencing of a [NiFe] hydrogenase operon from
Desulfovibrio vulgaris Miyazaki F
JOURNAL J. Gen. Microbiol. 136, 2021-2028 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
RBS 207..212
/note="small subunit ribosome binding site"
/gene="hyd"
CDS 219..1172
/product="[NiFe] hydrogenase small subunit"
/gene="hyd"
/codon_start=219
sig_peptide 219..368
/gene="hyd"
/codon_start=219
mat_peptide 369..1160
/gene="hyd"
/codon_start=369
RBS 1219..1224
/note="large subunit ribosome binding site"
/gene="hyd"
CDS 1231..2934
/product="[NiFe] hydrogenase large subunit"
/gene="hyd"
/codon_start=1231
terminator 2987..3013
/note="inverted repeat"
/gene="hyd"
BASE COUNT 620 a 1090 c 927 g 463 t
ORIGIN
1 cgcgatgcgg aaatgtgtgc ctcatttaca ttttcaattg gaataacgtt tcaatgtgtt
61 gtaccttccc ggcaaacgtg cggacaggtc accgcgggaa acggatgcac gcagaagcgg
121 tcgcagccgg ggggcctttc gcaccagtac gacccttacc ccgccgcgcc gctggcggcg
181 ggcaggcacg gtgatacgcc gtgccgaagg aggcagggat gaaaatctcg atcggtctcg
241 gcaaggaggg cgtggaggaa aggcttgcgg aacgcggcgt gtctcgacgc gacttcctca
301 agttctgtac ggccatcgcc gtgaccatgg gcatgggccc cgcgttcgcg ccggaagttg
361 cccgcgcgct catgggtccc cggcgcccgt ccgtggtcta cctgcacaac gccgaatgca
421 ccggctgttc cgaatcggtg ctgcgcgcgt tcgaacccta catcgacacc ctgattctgg
481 acacgctgtc cctcgactac catgagacca tcatggccgc cgcgggcgat gcggcggaag
541 ccgccctgga gcaggccgtc aacagcccgc acggcttcat cgccgtggtg gaaggcggca
601 ttcccacggc tgccaacggc atctacggca aggtggccaa ccacaccatg ctggacatct
661 gcagccgcat cctgcccaag gcccaggccg tcatcgcgta cggcacctgc gccaccttcg
721 gcggcgtgca ggcagccaag cccaacccca ccggtgccaa gggcgtcaac gacgcgctga
781 agcaccttgg cgtcaaggcc atcaacatcg ccggttgccc gccgaacccg tacaacctgg
841 tcggcaccat cgtgtactac ctgaagaaca aggccgcgcc cgagctggac agcctgaacc
901 ggcccaccat gttcttcggc cagaccgtgc acgaacagtg cccccgccta ccgcacttcg
961 acgcgggcga attcgccccg tcgttcgaat cggaagaagc ccgcaagggc tggtgcctct
1021 acgagctggg ctgcaagggc ccggtgacca tgaacaactg cccgaagatc aagttcaacc
1081 agaccaactg gcccgtggac gcggggcacc cctgcatcgg gtgcagcgaa cccgatttct
1141 gggacgccat gaccccgttc taccagaact gatcacgcgc acgcgctagg tcacccaata
1201 cccccatagg caacagccaa ggagaatacc atgagcggct gcagagccca gaatgctccg
1261 ggcggcatcc ccgtgacgcc caagagctcc tatagcggtc ccatcgtcgt cgaccccgtt
1321 acccgcatcg aaggccacct gcgcatcgag gtggaagtgg agaacggcaa ggtcaagaac
1381 gcctacagca gttccacgct gttccggggc cttgaaatca tcctgaaggg ccgcgacccc
1441 cgcgacgccc agcacttcac ccagcgcacc tgcggcgtgt gcacctatac ccatgcgctg
1501 gcctccaccc gctgcgtgga caacgccgtg ggcgtgcaca ttcccaagaa cgccacctac
1561 atccgcaacc tggtgctggg cgcgcagtac ctgcacgacc acatcgtgca cttctaccac
1621 ctgcacgccc tggacttcgt ggacgtgacc gccgcgctga aggccgaccc ggccaaggcc
1681 gccaaggtgg cctcgtccat ctcgccccgc aagaccacgg cggcggacct gaaggcggtg
1741 caggacaagc tgaagacctt cgtggaaacc gggcagctcg gcccgttcac caatgcctac
1801 ttcctgggcg gccaccccgc ctactacctg gatccggaaa ccaacctcat cgccaccgcc
1861 cactacctgg aagccctgcg ccttcaggtg aaggccgcgc gcgccatggc cgttttcggc
1921 gccaagaacc cgcacaccca gttcaccgtg gtgggcggcg tgacctgcta cgacgccctg
1981 accccgcagc gcatcgccga attcgaggcg ctgtggaagg aaaccaaggc gttcgttgat
2041 gaagtgtaca tccccgacct gctggtggtt gccgcggcct acaaggattg gacgcagtac
2101 ggcggcaccg acaacttcat caccttcggc gaattcccga aggacgagta cgacctgaac
2161 agccggttct tcaagccggg cgtggtcttc aagcgcgact tcaagaacat caagccgttc
2221 gacaagatgc agatcgaaga acacgtgcgc cacagctggt acgaaggcgc ggaagcccgc
2281 cacccgtgga agggccagac ccagcccaag tacaccgacc tgcacggcga cgaccgctac
2341 tcgtggatga aggccccccg ctacatgggc gagcccatgg aaacggggcc gctggcccag
2401 gtgctgatcg cctactcgca gggccacccc aaggtgaagg ccgtgaccga cgcagtgctt
2461 gccaagctgg gcgtgggccc cgaggccctg ttctccaccc tgggccgcac ggcggcgcgc
2521 ggcatcgaaa ccgccgtcat cgcagaatac gtgggagtga tgctgcagga gtacaaggac
2581 aacatcgcca agggcgacaa cgtcatctgc gccccgtggg aaatgcccaa gcaggcggaa
2641 ggcgtgggct tcgtcaacgc cccgcgcggc ggcctgtcgc actggatccg catcgaggac
2701 ggcaagatcg gcaacttcca actggtcgtg ccctccacct ggacccttgg cccccgctgc
2761 gacaagaaca acgtgtcgcc ggtggaagcc tcgctcatcg gcaccccggt ggccgatgcc
2821 aagcgcccgg tggaaatcct gcgcacggtg cattcgttcg acccgtgcat cgcctgcggc
2881 gtgcacgtca tcgacgggca caccaacgaa gtgcacaagt tccgcatcct gtaagctgtc
2941 cgcagccccg cctgccaagg cggcgtgccg gaatgtcata tcgaccgaag gcccggcctt
3001 gtgccgggcc ctcggactat ctgagaagct ggcacagcgg cagccccgcc tgtgcaccgc
3061 ctaccggacg acggccccga aaactgcgcg tcaacggcgg
//