GenBank-Updates@genbank.bio.net (12/07/90)
LOCUS PSEMDLABC 5099 bp ds-DNA BCT 07-DEC-1990
DEFINITION Pseudomonas putida benzoylformate decarboxylase (mdlC), S-mandelate
dehydrogenase (mdlB), and mandelate racemase (mdlA) genes, complete
cds.
ACCESSION J05293
KEYWORDS S-mandelate dehydrogenase; benzoylformate decarboxylase;
mandelate racemase.
SOURCE Pseudomonas putida (strain: ATCC 12633) DNA.
ORGANISM Pseudomonas putida
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 5099)
AUTHORS Tsou,A.Y., Ransom,S.C., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and
Kenyon,G.L.
TITLE Mandelate pathway of Pseudomonas putida: Sequence relationships
involoving mandelate racemase, (S)-mandelate dehydrogenase, and
benzoylformate decarboxylase and expression of benzoylformate
decarboxylase in Escherichia coli
JOURNAL Biochemistry 29, 9856-9862 (1990)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [Biochemistry (1990)
In press] kindly submitted by A.Y.Tsou, 27-AUG-1990.
FEATURES from to/span description
pept 656 2155 benzoylformate decarboxylase (mdlC)
/EC_number="4.1.1.7"
pept 2249 3430 S-mandelate dehydrogenase (mdlB),
pept 3427 4506 mandelate racemase (mdlA) /EC_number="5.1.2.2"
BASE COUNT 1198 a 1425 c 1345 g 1131 t
ORIGIN
1 aagctttctg aacatatggc tggagggtcg atagcagacg ttcttctctc tcgctgaagg
61 gtttgctccc cttgctcctc cataccctga gatcgccgat gtcccgatca ccgtcgaaga
121 aaaagacgtt gaggccatgc tccatatctg ccggctgaag gaagtcgttg tagaactcgc
181 tcttgagcat tgctgaacgt ggcacatggc agtcgactcc gccggcgccc tcacctctcg
241 catgatcggg gttaccaaat cgacctggta aaagtgcttc tgataagcct ctatgaactg
301 gctctccata ttgaaagcca gcgggtcgat ggatcgcccc tcacttttgt cccatacgaa
361 tgaagcaacg aactccgcgt cgagaaaggc cgctaccgct tgcaaaatcg cagcccgagc
421 gtttggtttt ccctggaact gagcaagaag ccagaccagc tcagcaagct gatccatatc
481 ggttccacgc agcactgttg ccattgccta cctccgaaaa tgctgtcaaa gcgtcacaga
541 agggtaaaag catcaaaata gaaccgatct atatattatt ttagaatcta tctatttgat
601 ctatttaggt ctcgatttta tcgtgggatt caccatttgg taagagataa aaatcatggc
661 ttcggtacac ggcaccacat acgaactctt gcgacgtcaa ggcatcgata cggtcttcgg
721 caatcctggc tcgaacgagc tcccgttttt gaaggacttt ccagaggact ttcgatacat
781 cctggctttg caggaagcgt gtgtggtggg cattgcagac ggctatgcgc aagccagtcg
841 gaagccggct ttcattaacc tgcattctgc tgctggtacc ggcaatgcta tgggtgcact
901 cagtaacgcc tggaactcac attccccgct gatcgtcact gccggccagc agaccagggc
961 gatgattggc gttgaagctc tgctgaccaa cgtcgatgcc gccaacctgc cacgaccact
1021 tgtcaaatgg agctacgagc ccgcaagcgc agcagaagtc cctcatgcga tgagcagggc
1081 tatccatatg gcaagcatgg cgccacaagg ccctgtctat ctttcggtgc catatgacga
1141 ttgggataag gatgctgatc ctcagtccca ccaccttttt gatcgccatg tcagttcatc
1201 agtacgcctg aacgaccagg atctcgatat tctggtgaaa gctctcaaca gcgcatccaa
1261 cccggcgatc gtcctgggcc cggacgtcga cgcagcaaat gcgaacgcag actgcgtcat
1321 gttggccgaa cgcctcaaag ctccggtttg ggttgcgcca tccgctccac gctgcccatt
1381 ccctacccgt catccttgct tccgtggatt gatgccagct ggcatcgcag cgatttctca
1441 gctgctcgaa ggtcacgatg tggttttggt aatcggcgct ccagtgttcc gttaccacca
1501 atacgaccca ggtcaatatc tcaaacctgg cacgcgattg atttcggtga cctgcgaccc
1561 gctcgaagct gcacgcgcgc caatgggcga tgcgatcgtg gcagacattg gtgcgatggc
1621 tagcgctctt gccaacttgg ttgaagagag cagccgccag ctcccaactg cagctccgga
1681 acccgcgaag gttgaccaag acgctggccg acttcaccca gagacagtgt tcgacacact
1741 gaacgacatg gccccggaga atgcgattta cctgaacgag tcgacttcaa cgaccggcaa
1801 atgtgcagcg ctgaacatgc gcaaccctgg tagctactac ttctgtgcag ctggcggact
1861 gggcttcgcc ctgcctgcag caattggcgt tcaactcgca gaacccgagc gacaagtcat
1921 cgccgtcatt ggcgacggat cggcgaacta cagcattagt gcgttgtgga ctgcagctca
1981 gtacaacatc cccactatct tcgtgatcat gaacaacggc acctacggtc ggtttgcgat
2041 ggtttgccgg cgttctcgaa gcagaaaacg ttcctgggct ggatgtgcca gggatcgact
2101 tccgcgcact cgccaagggc tatggtgtcc aagcgctgaa agccgacaac cttgagcagc
2161 tcaagggttc gctacaagaa gcgctttctg ccaaaggccc ggtacttatc gaagtaagca
2221 ccgtaagccc ggtgaagtga ggtaagccat gagccagaat ctctttaacg ttgaggacta
2281 tcgcaagctt cggcaaaagc gcttgccgaa gatggtctac gactatctgg aaggtggggc
2341 tgaagacgaa tacggggtga aacacaaccg cgacgtcttc cagcaatggc gattcaaacc
2401 gaagcggcta gtagacgtca gccgccgcag cctccaagcg gaagtacttg gaaagaggca
2461 gtcgatgcct ctcttgattg ggcctactgg gctgaacggt gcgctgtggc ctaaggggga
2521 tctcgcttta gctcgagcag caaccaaggc cggaatcccg ttcgtgctgt cgaccgcctc
2581 caacatgtcc attgaagacc tcgcacgtca gtgtgatggc gatctgtggt tccagctcta
2641 tgtgatccac cgagagattg cgcaggggat ggtgctcaaa gccctgcaca ctggttacac
2701 gacactggtg cttactacgg atgtggcggt taacggctat cgcgagcgcg acctgcataa
2761 ccgattcaag ataccaatga gctactccgc aaaggtggtg ctggacggat gcctgcatcc
2821 gcgctggtcg ctcgacttcg tgcgacacgg catgccgcaa ctggccaatt tcgtcagcag
2881 tcaaacgtct agcttagaaa tgcaggcagc attgatgagc cgccaaatgg atgccagttt
2941 caactgggag gcattgagat ggctgcgtga cctctggccg cacaaactcc tcgtaaaggg
3001 gttgctcagt gctgaggacg ccgatcggtg catcgctgaa ggtgcagacg gcgtaatcct
3061 atcaaaccac ggcggtcgcc aactcgattg cgcgatatcg ccaatggaag ttttggctca
3121 atcggtagcg aaaactggaa aaccagtgct tatcgatagc ggcttccgac ggggttcgga
3181 catcgttaaa gcacttgcgc taggtgctga ggctgtactc ctgggtcgtg caactttgta
3241 tggccttgca gcacgaggtg aaacgggtgt tgacgaggtg ctaaccctcc taaaagcgga
3301 tatcgaccgc acccttgccc agattggatg ccctgacatc acctcccttt ctcctgatta
3361 cctccaaaac gagggagtga ctaacaccgc tccagtcgat cacctcattg gtaaaggaac
3421 acacgcatga gtgaagtact gattaccggc ctgaggacgc gggccgtcaa tgtcccattg
3481 gcctaccccg ttcacaccgc tgttggaact gttggcacag cgcctcttgt tctgatcgac
3541 ctggccacca gtgccggcgt ggtaggccat tcctacctgt tcgcatacac ccccgttgcg
3601 ttgaagtcgc tgaagcagct cctggacgac atggcagcca tgatcgtcaa cgaaccattg
3661 gcaccggtta gcttggaagc catgctcgca aaacgcttct gcctggcagg ttatacggga
3721 ttgatccgaa tggccgctgc tggcatcgat atggcagctt gggatgcgct gggcaaggtt
3781 cacgaaacgc cactagtaaa actcctcggc gcgaacgctc gaccagttca ggcttatgac
3841 agccacagct tggatggagt caaactcgcg actgagcgcg ctgtgaccgc agcagaactc
3901 ggattccggg cggttaagac caagatcggc tatccggcat tggatcaaga tctggcagtc
3961 gtgcgcagca tacgccaagc agtaggtgac gacttcggca tcatggtcga ctacaaccag
4021 agtttggatg taccagccgc aatcaaacgc agccaggcgc tgcagcaaga gggcgtcacc
4081 tggattgaag agccgacgct tcaacacgat tacgaaggcc atcagcgcat tcaaagcaag
4141 ctcaatgtgc ccgtccagat gggtgagaac tggctcggcc ctgaggagat gttcaaagca
4201 ttgagcatcg gtgcatgccg gttggctatg ccagatgcaa tgaagatcgg tggcgtgacg
4261 ggctggatcc gagccagtgc actcgcacaa caattcggta ttccaatgtc cagccacctg
4321 ttccaagaaa tcagcgctca cctgctagcc gccaccccaa ctgcgcattg gctggagcgt
4381 ttggatctcg ccggcagcgt catcgagcct actcttacat ttgagggcgg aaatgctgtg
4441 attccggatc tccctggcgt tgggatcatc tggcgcgaga aagaaatcgg gaaatatctg
4501 gtgtaatgca aaataaaaag gcccgcttat gcgggccttt tcttatacag actttccatg
4561 ctttagcgtt tcggaaggta gctctgaaaa cctggcttta tattcagccg agaaacggcc
4621 caaatgcgta aaaccgtaat ccatcgcgag ttcagttaca ctcttttttc gaccgtcagc
4681 attcaacaaa gccgcacgta tccgatctag tttcctgctg acaaaatacc gacggggtgg
4741 ctcgcccatg tgctgctcga agagaacgta gaggctgcgc acacttacgt tagcataacg
4801 agccaacgtt tcaggtgtca aatcttgctt caaattatcc tcaatatatt gcagcacctt
4861 gcgcaaaact tgactctgca ctccctcccc ctttacatcc aggttagtca aaaaagaagt
4921 aagtgctttg ataattataa tttgaacgta atgctcttga accgcacgaa tatgttctca
4981 ctttcaatct cgttacagac aaggctcaac agctgcgcaa tcccttccag gtcgattagc
5041 ttgtatactt tatcctcaaa acgtaggcct tcggctggca gacaccagcg ctggagctc
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS PSEMDLABC 5099 bp ds-DNA BCT 31-JAN-1991
DEFINITION Pseudomonas putida benzoylformate decarboxylase (mdlC), S-mandelate
dehydrogenase (mdlB), and mandelate racemase (mdlA) genes, complete
cds.
ACCESSION J05293
KEYWORDS S-mandelate dehydrogenase; benzoylformate decarboxylase;
mandelate racemase.
SOURCE Pseudomonas putida (strain: ATCC 12633) DNA.
ORGANISM Pseudomonas putida
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 5099)
AUTHORS Tsou,A.Y., Ransom,S.C., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and
Kenyon,G.L.
TITLE Mandelate pathway of Pseudomonas putida: Sequence relationships
involoving mandelate racemase, (S)-mandelate dehydrogenase, and
benzoylformate decarboxylase and expression of benzoylformate
decarboxylase in Escherichia coli
JOURNAL Biochemistry 29, 9856-9862 (1990)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [Biochemistry (1990)
In press] kindly submitted
by A.Y.Tsou, 27-AUG-1990.
FEATURES Location/Qualifiers
CDS 656..2155
/EC_number="4.1.1.7"
/product="benzoylformate decarboxylase"
/gene="mdlC"
/codon_start=656
CDS 2249..3430
/product="S-mandelate dehydrogenase"
/gene="mdlB"
/codon_start=2249
CDS 3427..4506
/EC_number="5.1.2.2"
/product="mandelate racemase"
/gene="mdlA"
/codon_start=3427
BASE COUNT 1198 a 1425 c 1345 g 1131 t
ORIGIN
1 aagctttctg aacatatggc tggagggtcg atagcagacg ttcttctctc tcgctgaagg
61 gtttgctccc cttgctcctc cataccctga gatcgccgat gtcccgatca ccgtcgaaga
121 aaaagacgtt gaggccatgc tccatatctg ccggctgaag gaagtcgttg tagaactcgc
181 tcttgagcat tgctgaacgt ggcacatggc agtcgactcc gccggcgccc tcacctctcg
241 catgatcggg gttaccaaat cgacctggta aaagtgcttc tgataagcct ctatgaactg
301 gctctccata ttgaaagcca gcgggtcgat ggatcgcccc tcacttttgt cccatacgaa
361 tgaagcaacg aactccgcgt cgagaaaggc cgctaccgct tgcaaaatcg cagcccgagc
421 gtttggtttt ccctggaact gagcaagaag ccagaccagc tcagcaagct gatccatatc
481 ggttccacgc agcactgttg ccattgccta cctccgaaaa tgctgtcaaa gcgtcacaga
541 agggtaaaag catcaaaata gaaccgatct atatattatt ttagaatcta tctatttgat
601 ctatttaggt ctcgatttta tcgtgggatt caccatttgg taagagataa aaatcatggc
661 ttcggtacac ggcaccacat acgaactctt gcgacgtcaa ggcatcgata cggtcttcgg
721 caatcctggc tcgaacgagc tcccgttttt gaaggacttt ccagaggact ttcgatacat
781 cctggctttg caggaagcgt gtgtggtggg cattgcagac ggctatgcgc aagccagtcg
841 gaagccggct ttcattaacc tgcattctgc tgctggtacc ggcaatgcta tgggtgcact
901 cagtaacgcc tggaactcac attccccgct gatcgtcact gccggccagc agaccagggc
961 gatgattggc gttgaagctc tgctgaccaa cgtcgatgcc gccaacctgc cacgaccact
1021 tgtcaaatgg agctacgagc ccgcaagcgc agcagaagtc cctcatgcga tgagcagggc
1081 tatccatatg gcaagcatgg cgccacaagg ccctgtctat ctttcggtgc catatgacga
1141 ttgggataag gatgctgatc ctcagtccca ccaccttttt gatcgccatg tcagttcatc
1201 agtacgcctg aacgaccagg atctcgatat tctggtgaaa gctctcaaca gcgcatccaa
1261 cccggcgatc gtcctgggcc cggacgtcga cgcagcaaat gcgaacgcag actgcgtcat
1321 gttggccgaa cgcctcaaag ctccggtttg ggttgcgcca tccgctccac gctgcccatt
1381 ccctacccgt catccttgct tccgtggatt gatgccagct ggcatcgcag cgatttctca
1441 gctgctcgaa ggtcacgatg tggttttggt aatcggcgct ccagtgttcc gttaccacca
1501 atacgaccca ggtcaatatc tcaaacctgg cacgcgattg atttcggtga cctgcgaccc
1561 gctcgaagct gcacgcgcgc caatgggcga tgcgatcgtg gcagacattg gtgcgatggc
1621 tagcgctctt gccaacttgg ttgaagagag cagccgccag ctcccaactg cagctccgga
1681 acccgcgaag gttgaccaag acgctggccg acttcaccca gagacagtgt tcgacacact
1741 gaacgacatg gccccggaga atgcgattta cctgaacgag tcgacttcaa cgaccggcaa
1801 atgtgcagcg ctgaacatgc gcaaccctgg tagctactac ttctgtgcag ctggcggact
1861 gggcttcgcc ctgcctgcag caattggcgt tcaactcgca gaacccgagc gacaagtcat
1921 cgccgtcatt ggcgacggat cggcgaacta cagcattagt gcgttgtgga ctgcagctca
1981 gtacaacatc cccactatct tcgtgatcat gaacaacggc acctacggtc ggtttgcgat
2041 ggtttgccgg cgttctcgaa gcagaaaacg ttcctgggct ggatgtgcca gggatcgact
2101 tccgcgcact cgccaagggc tatggtgtcc aagcgctgaa agccgacaac cttgagcagc
2161 tcaagggttc gctacaagaa gcgctttctg ccaaaggccc ggtacttatc gaagtaagca
2221 ccgtaagccc ggtgaagtga ggtaagccat gagccagaat ctctttaacg ttgaggacta
2281 tcgcaagctt cggcaaaagc gcttgccgaa gatggtctac gactatctgg aaggtggggc
2341 tgaagacgaa tacggggtga aacacaaccg cgacgtcttc cagcaatggc gattcaaacc
2401 gaagcggcta gtagacgtca gccgccgcag cctccaagcg gaagtacttg gaaagaggca
2461 gtcgatgcct ctcttgattg ggcctactgg gctgaacggt gcgctgtggc ctaaggggga
2521 tctcgcttta gctcgagcag caaccaaggc cggaatcccg ttcgtgctgt cgaccgcctc
2581 caacatgtcc attgaagacc tcgcacgtca gtgtgatggc gatctgtggt tccagctcta
2641 tgtgatccac cgagagattg cgcaggggat ggtgctcaaa gccctgcaca ctggttacac
2701 gacactggtg cttactacgg atgtggcggt taacggctat cgcgagcgcg acctgcataa
2761 ccgattcaag ataccaatga gctactccgc aaaggtggtg ctggacggat gcctgcatcc
2821 gcgctggtcg ctcgacttcg tgcgacacgg catgccgcaa ctggccaatt tcgtcagcag
2881 tcaaacgtct agcttagaaa tgcaggcagc attgatgagc cgccaaatgg atgccagttt
2941 caactgggag gcattgagat ggctgcgtga cctctggccg cacaaactcc tcgtaaaggg
3001 gttgctcagt gctgaggacg ccgatcggtg catcgctgaa ggtgcagacg gcgtaatcct
3061 atcaaaccac ggcggtcgcc aactcgattg cgcgatatcg ccaatggaag ttttggctca
3121 atcggtagcg aaaactggaa aaccagtgct tatcgatagc ggcttccgac ggggttcgga
3181 catcgttaaa gcacttgcgc taggtgctga ggctgtactc ctgggtcgtg caactttgta
3241 tggccttgca gcacgaggtg aaacgggtgt tgacgaggtg ctaaccctcc taaaagcgga
3301 tatcgaccgc acccttgccc agattggatg ccctgacatc acctcccttt ctcctgatta
3361 cctccaaaac gagggagtga ctaacaccgc tccagtcgat cacctcattg gtaaaggaac
3421 acacgcatga gtgaagtact gattaccggc ctgaggacgc gggccgtcaa tgtcccattg
3481 gcctaccccg ttcacaccgc tgttggaact gttggcacag cgcctcttgt tctgatcgac
3541 ctggccacca gtgccggcgt ggtaggccat tcctacctgt tcgcatacac ccccgttgcg
3601 ttgaagtcgc tgaagcagct cctggacgac atggcagcca tgatcgtcaa cgaaccattg
3661 gcaccggtta gcttggaagc catgctcgca aaacgcttct gcctggcagg ttatacggga
3721 ttgatccgaa tggccgctgc tggcatcgat atggcagctt gggatgcgct gggcaaggtt
3781 cacgaaacgc cactagtaaa actcctcggc gcgaacgctc gaccagttca ggcttatgac
3841 agccacagct tggatggagt caaactcgcg actgagcgcg ctgtgaccgc agcagaactc
3901 ggattccggg cggttaagac caagatcggc tatccggcat tggatcaaga tctggcagtc
3961 gtgcgcagca tacgccaagc agtaggtgac gacttcggca tcatggtcga ctacaaccag
4021 agtttggatg taccagccgc aatcaaacgc agccaggcgc tgcagcaaga gggcgtcacc
4081 tggattgaag agccgacgct tcaacacgat tacgaaggcc atcagcgcat tcaaagcaag
4141 ctcaatgtgc ccgtccagat gggtgagaac tggctcggcc ctgaggagat gttcaaagca
4201 ttgagcatcg gtgcatgccg gttggctatg ccagatgcaa tgaagatcgg tggcgtgacg
4261 ggctggatcc gagccagtgc actcgcacaa caattcggta ttccaatgtc cagccacctg
4321 ttccaagaaa tcagcgctca cctgctagcc gccaccccaa ctgcgcattg gctggagcgt
4381 ttggatctcg ccggcagcgt catcgagcct actcttacat ttgagggcgg aaatgctgtg
4441 attccggatc tccctggcgt tgggatcatc tggcgcgaga aagaaatcgg gaaatatctg
4501 gtgtaatgca aaataaaaag gcccgcttat gcgggccttt tcttatacag actttccatg
4561 ctttagcgtt tcggaaggta gctctgaaaa cctggcttta tattcagccg agaaacggcc
4621 caaatgcgta aaaccgtaat ccatcgcgag ttcagttaca ctcttttttc gaccgtcagc
4681 attcaacaaa gccgcacgta tccgatctag tttcctgctg acaaaatacc gacggggtgg
4741 ctcgcccatg tgctgctcga agagaacgta gaggctgcgc acacttacgt tagcataacg
4801 agccaacgtt tcaggtgtca aatcttgctt caaattatcc tcaatatatt gcagcacctt
4861 gcgcaaaact tgactctgca ctccctcccc ctttacatcc aggttagtca aaaaagaagt
4921 aagtgctttg ataattataa tttgaacgta atgctcttga accgcacgaa tatgttctca
4981 ctttcaatct cgttacagac aaggctcaac agctgcgcaa tcccttccag gtcgattagc
5041 ttgtatactt tatcctcaaa acgtaggcct tcggctggca gacaccagcg ctggagctc
//