GenBank-Updates@genbank.bio.net (11/30/90)
LOCUS SP1DNAPOL 1157 bp ds-DNA PHG 30-NOV-1990
DEFINITION Bacteriophage SPO1 gene 31, exons 1 and 2, partial cds, and intron
ORF.
ACCESSION M37686
KEYWORDS DNA polymerase; group I self-splicing intron.
SOURCE Bacteriophage SPO1 DNA, clone pJK9 and subclones.
ORGANISM Bacteriophage SPO1
Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE 1 (bases 1 to 1157)
AUTHORS Goodrich-Blair,H., Scarlato,V., Gott,J.M., Xu,M.-Q. and Shub,D.A.
TITLE A self-splicing group I intron in the DNA polymerase gene of
Bacillus subtilis bacteriophage SPO1
JOURNAL Cell 63, 417-424 (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by D.A.Shub, 09-AUG-1990.
FEATURES from to/span description
pept < 1 135 DNA polymerase, exon 1 (EC 2.7.7.7; AA at 1)
1019 > 1156 DNA polymerase, exon 2
pept 443 967 intron ORF
IVS 136 1018 DNA polymerase intron 1 (no splice consensus)
site 249 260 P sequence
site 288 297 Q sequence
site 318 331 R sequence
signal 428 437 ribosome binding site
site 967 978 S sequence
BASE COUNT 397 a 192 c 285 g 283 t
ORIGIN
1 gaattccgta acggaaacca cttatataat aactttgtta gtaaactgtc tctgatgata
61 gaccctgata acattgttca ccctagctac aacatacatg gcactgtgac aggtcgtttg
121 agtagtaatg agcctaaaga agttgggcac cgctatggta acatagcgtg ttaaacccag
181 tgaacctata aatatagggt gtatactcca cgtgcaggag tgtcaggaaa tgggcactag
241 ggagcgtgct aacaggggaa ctgagtaggg atgagcgcct acacagcaat cctgtgccaa
301 gaccctttga gggtaaggtg caacgactat cgaaaccact cagaaatgag gaagggagta
361 gagtacctta caggtgaaac tcctgtaggg gaagcgctgg gcaaccgttg agatatgtat
421 aaaacataag gaggtgaaga atatggaatg gaaggacatt aaaggatatg aggggcacta
481 tcaagtatca aacaccgggg aagtatacag cataaagtcg ggaaagactt taaaacatca
541 gattcctaaa gatgggtatc acaggattgg actttttaaa ggtggaaaag ggaaaacgtt
601 tcaagtgcat cgtctggtgg cgattcactt ttgtgaagga tatgaggaag gtctagtagt
661 tgatcataaa gatggtaaca aggacaacaa tctcagtaca aatttaagat gggttaccca
721 aaagataaac gtagagaatc aaatgtctag agggacttta aacgtatcta aggctcaaca
781 aatcgccaag ataaaaaacc agaagccaat cattgtgatc tctccagatg gaattgagaa
841 agagtatcca tcaactaagt gtgcttgtga agaattggga ttaacaagag gtaaagtaac
901 tgatgtcctg aagggacata ggattcacca caagggatac acttttaggt acaaactcaa
961 cggttgaaga tatagtctag caggtagtat aatcggggaa tttttatact acttgtagaa
1021 cgctcagcaa ttcccacgta aggtgaacac gccaacatta ttccagtata actttgagat
1081 taagaaaatg tttaactcta ggtttgggga tggtggtgta attgtacagt ttgactactc
1141 tcagttagag ttacgta
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS SP1DNAPOL 1157 bp ds-DNA PHG 31-JAN-1991
DEFINITION Bacteriophage SPO1 gene 31, exons 1 and 2, partial cds, and intron
ORF.
ACCESSION M37686
KEYWORDS DNA polymerase; group I self-splicing intron.
SOURCE Bacteriophage SPO1 DNA, clone pJK9 and subclones.
ORGANISM Bacteriophage SPO1
Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE 1 (bases 1 to 1157)
AUTHORS Goodrich-Blair,H., Scarlato,V., Gott,J.M., Xu,M.-Q. and Shub,D.A.
TITLE A self-splicing group I intron in the DNA polymerase gene of
Bacillus subtilis bacteriophage SPO1
JOURNAL Cell 63, 417-424 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Cell (1990) In
press] kindly submitted
by D.A.Shub, 09-AUG-1990.
FEATURES Location/Qualifiers
exon <1..135
/number=1
/EC_number="E.C. 2.7.7.7"
/product="DNA polymerase"
/gene="gene 31"
CDS join(<1..135,1019..>1156)
/EC_number="2.7.7.7"
/product="DNA polymerase"
/gene="gene 31"
/codon_start=1
/partial
intron 136..1018
/number=1
/EC_number="E.C. 2.7.7.7"
/gene="gene 31"
misc_feature 249..260
/note="P sequence"
misc_feature 288..297
/note="Q sequence"
misc_feature 318..331
/note="R sequence"
RBS 428..437
/note="Shine-Delgarno sequence"
CDS 443..967
/note="intron ORF"
/codon_start=443
misc_feature 967..978
/note="S sequence"
exon 1019..>1156
/number=2
/EC_number="E.C. 2.7.7.7"
/product="DNA polymerase"
/gene="gene 31"
BASE COUNT 397 a 192 c 285 g 283 t
ORIGIN
1 gaattccgta acggaaacca cttatataat aactttgtta gtaaactgtc tctgatgata
61 gaccctgata acattgttca ccctagctac aacatacatg gcactgtgac aggtcgtttg
121 agtagtaatg agcctaaaga agttgggcac cgctatggta acatagcgtg ttaaacccag
181 tgaacctata aatatagggt gtatactcca cgtgcaggag tgtcaggaaa tgggcactag
241 ggagcgtgct aacaggggaa ctgagtaggg atgagcgcct acacagcaat cctgtgccaa
301 gaccctttga gggtaaggtg caacgactat cgaaaccact cagaaatgag gaagggagta
361 gagtacctta caggtgaaac tcctgtaggg gaagcgctgg gcaaccgttg agatatgtat
421 aaaacataag gaggtgaaga atatggaatg gaaggacatt aaaggatatg aggggcacta
481 tcaagtatca aacaccgggg aagtatacag cataaagtcg ggaaagactt taaaacatca
541 gattcctaaa gatgggtatc acaggattgg actttttaaa ggtggaaaag ggaaaacgtt
601 tcaagtgcat cgtctggtgg cgattcactt ttgtgaagga tatgaggaag gtctagtagt
661 tgatcataaa gatggtaaca aggacaacaa tctcagtaca aatttaagat gggttaccca
721 aaagataaac gtagagaatc aaatgtctag agggacttta aacgtatcta aggctcaaca
781 aatcgccaag ataaaaaacc agaagccaat cattgtgatc tctccagatg gaattgagaa
841 agagtatcca tcaactaagt gtgcttgtga agaattggga ttaacaagag gtaaagtaac
901 tgatgtcctg aagggacata ggattcacca caagggatac acttttaggt acaaactcaa
961 cggttgaaga tatagtctag caggtagtat aatcggggaa tttttatact acttgtagaa
1021 cgctcagcaa ttcccacgta aggtgaacac gccaacatta ttccagtata actttgagat
1081 taagaaaatg tttaactcta ggtttgggga tggtggtgta attgtacagt ttgactactc
1141 tcagttagag ttacgta
//