GenBank-Updates@genbank.bio.net (12/05/90)
LOCUS STYPRF2 1351 bp ds-DNA BCT 05-DEC-1990
DEFINITION S.typhimurium peptide chain-release-factor 2 (prfB) gene, complete
cds, and HerC gene product, 5' end.
ACCESSION M38590
KEYWORDS HerC product; peptide chain release factor 2.
SOURCE S.typhimurium (sub-species LT2) DNA.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1351)
AUTHORS Kawakami,K. and Nakamura,Y.
TITLE Autogenous suppression of an opal mutation in the gene encoding
peptide chain release factor 2
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 8432-8436 (1990)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by K.Kawakami, 17-SEP-1990.
FEATURES from to/span description
pept < 1 46 unknown protein (AA at 2)
pept 151 225 peptide chain release factor 2
227 1249 peptide chain release factor 2
pept 1259 > 1351 HerC product
BASE COUNT 365 a 319 c 386 g 281 t
ORIGIN
1 caaccgtagt ttacagatta ttatcgatga tatttggccg ctatgatcgg ggcgatttaa
61 caacactttc gctgtaatta aggggcgaaa tccgctaata tttcgccctt ataaccgcat
121 tttgacaagt caaataaaag aaatcagacc atgtttgaaa ttaacccggt gaataaccgc
181 attcaggacc tcacggaacg caccaacgtt cttagggggt atctttgact acgatgctaa
241 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
301 tgagcgcgcg caggcgctgg gaaaagagcg ttcatcgctc gaagcgatcg tcgatacgct
361 tgatcaaatg actcaggggc tggacgacgt ttccgggctg ctggaactgg cggtagaagc
421 tgacgacgaa gagacgttta acgaagccgt cgcggaactg aatacgctgg aagagaagtt
481 ggcgcagttg gaattccgcc ggatgttctc cggcgagtac gatagcgccg attgctatct
541 cgatattcag gccggttccg gtggtactga agcgcaggac tgggccagca tgttgctgcg
601 tatgtatctg cgttgggcgg aagcgcgcgg cttcaagaca gaagtcattg aagaatcgga
661 aggggaagtc gccggtatta agtccgcgac tatcaaaatc tccggcgaat atgcctatgg
721 ctggctgcgc actgaaaccg gcgtacatcg tctggtgcgt aaaagcccgt ttgactccgg
781 cggtcgccgt catacctcgt ttagctccgc gtttgtgtac ccggaagtgg acgacgatat
841 cgatatcgac attaacccgg cggatctgcg tatcgacgtc tatcgcgcgt ctggcgcagg
901 cggtcagcac gttaaccgta cggaatccgc cgtgcgtatc acccatattc caaccggaat
961 cgtgacgcag tgccagaacg accgttcgca gcacaaaaac aaagaccagg ccatgaagca
1021 gatgaaagcg aagctttatg aactggagat gcagaaaaag aacgctgaaa aacaggcgat
1081 ggaagacact aagtccgata tcggctgggg aagccagatt cgttcctatg tcctggacga
1141 ttcccgcatt aaagacctgc gtactggggt ggaaacccgc aatacacagg cggtactgga
1201 cggtagtctg gatcaattta tcgaagcaag tttgaaagca gggctatgag gaatcaacat
1261 gtctgaacaa aacgcacagg gcgctgacga ggtagtcgat cttaacaatg aaatgaaggc
1321 acgtcgcgag aagctggctg cactgcgtga g
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS STYPRF2 1351 bp ds-DNA BCT 31-JAN-1991
DEFINITION S.typhimurium peptide chain-release-factor 2 (prfB) gene, complete
cds, and HerC gene product, 5' end.
ACCESSION M38590
KEYWORDS HerC product; peptide chain release factor 2.
SOURCE S.typhimurium (sub-species LT2) DNA.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1351)
AUTHORS Kawakami,K. and Nakamura,Y.
TITLE Autogenous suppression of an opal mutation in the gene encoding
peptide chain release factor 2
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 8432-8436 (1990)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [Proc. Natl. Acad.
Sci. U.S.A. (1990) In press] kindly submitted
by K.Kawakami, 17-SEP-1990.
FEATURES Location/Qualifiers
CDS join(151..225,227..1249)
/product="peptide chain release factor 2"
/gene="prfB"
CDS <1..46
/codon_start=2
CDS 1259..>1351
/gene="HerC"
/codon_start=1259
BASE COUNT 365 a 319 c 386 g 281 t
ORIGIN
1 caaccgtagt ttacagatta ttatcgatga tatttggccg ctatgatcgg ggcgatttaa
61 caacactttc gctgtaatta aggggcgaaa tccgctaata tttcgccctt ataaccgcat
121 tttgacaagt caaataaaag aaatcagacc atgtttgaaa ttaacccggt gaataaccgc
181 attcaggacc tcacggaacg caccaacgtt cttagggggt atctttgact acgatgctaa
241 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
301 tgagcgcgcg caggcgctgg gaaaagagcg ttcatcgctc gaagcgatcg tcgatacgct
361 tgatcaaatg actcaggggc tggacgacgt ttccgggctg ctggaactgg cggtagaagc
421 tgacgacgaa gagacgttta acgaagccgt cgcggaactg aatacgctgg aagagaagtt
481 ggcgcagttg gaattccgcc ggatgttctc cggcgagtac gatagcgccg attgctatct
541 cgatattcag gccggttccg gtggtactga agcgcaggac tgggccagca tgttgctgcg
601 tatgtatctg cgttgggcgg aagcgcgcgg cttcaagaca gaagtcattg aagaatcgga
661 aggggaagtc gccggtatta agtccgcgac tatcaaaatc tccggcgaat atgcctatgg
721 ctggctgcgc actgaaaccg gcgtacatcg tctggtgcgt aaaagcccgt ttgactccgg
781 cggtcgccgt catacctcgt ttagctccgc gtttgtgtac ccggaagtgg acgacgatat
841 cgatatcgac attaacccgg cggatctgcg tatcgacgtc tatcgcgcgt ctggcgcagg
901 cggtcagcac gttaaccgta cggaatccgc cgtgcgtatc acccatattc caaccggaat
961 cgtgacgcag tgccagaacg accgttcgca gcacaaaaac aaagaccagg ccatgaagca
1021 gatgaaagcg aagctttatg aactggagat gcagaaaaag aacgctgaaa aacaggcgat
1081 ggaagacact aagtccgata tcggctgggg aagccagatt cgttcctatg tcctggacga
1141 ttcccgcatt aaagacctgc gtactggggt ggaaacccgc aatacacagg cggtactgga
1201 cggtagtctg gatcaattta tcgaagcaag tttgaaagca gggctatgag gaatcaacat
1261 gtctgaacaa aacgcacagg gcgctgacga ggtagtcgat cttaacaatg aaatgaaggc
1321 acgtcgcgag aagctggctg cactgcgtga g
//