GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS FSBACTBA 3994 bp ds-DNA VRT 31-JAN-1991
DEFINITION Common carp (C.carpio) beta-actin gene, complete cds.
ACCESSION M24113
KEYWORDS actin; beta-actin.
SOURCE Common carp (C.carpio) liver DNA, clone CA16.
ORGANISM Cyprinus carpio
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Osteichthyes;
Actinopterygii; Cypriniformes; Cyprinoidei; Cyprinidae.
REFERENCE 1 (bases 1 to 3994)
AUTHORS Liu,Z., Zhu,Z., Roberg,K., Faras,A., Guise,K., Kapuscinski,A.R. and
Hackett,P.B.
TITLE Isolation and characterization of beta-actin gene of carp (Cyprinus
carpio)
JOURNAL DNA Sequence 1, 125-136 (1990)
STANDARD simple staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1989)
Univ.Minnesota, St.Paul, MN 55108-1095] kindly submitted
by P.B.Hackett, 18-APR-1989.
FEATURES Location/Qualifiers
CDS join(1568..1690,1797..2036,
2340..2778,2872..3053,3155..3298)
/note="beta-actin"
prim_transcript 210..>3820
/note="beta-actin mRNA and introns"
intron 302..1565
/note="B-actin intron A"
misc_feature 1356..1404
/note="enhancer region"
exon 1568..1690
/note="beta-actin, exon 2 (first expressed exon)"
intron 1691..1796
/note="B-actin intron B"
exon 1797..2036
/note="beta-actin, exon 3"
intron 2037..2339
/note="B-actin intron C"
exon 2340..2778
/note="beta-actin, exon 4"
intron 2779..2871
/note="B-actin intron D"
exon 2872..3053
/note="beta-actin, exon 5"
intron 3054..3154
/note="B-actin intron E"
exon 3155..3298
/note="beta-actin, exon 6"
misc_feature 3780..3805
/note="B-actin downregulation sequence"
BASE COUNT 976 a 920 c 893 g 1205 t
ORIGIN 139 bp upstream of SstI site.
1 tttgatgaaa atcgcttagg ccttgctctt caaacaatcc agcttctcct tctttcactc
61 tcaagttgca agaagcaagt gtagcaatgt gcacgcgaca gccgggtgtg tgacgctgga
121 ccaatcagag cgcagagctc cgaaagttta ccttttatgg ctagagccgg catctgccgt
181 catataaaag agcgcgccca gcgtctcagc ctcactttga gctcctccac acgcagctag
241 tgcggaatat catctgcctg taacccattc tctaaagtcg acaaaccccc ccaaacctaa
301 ggtgagttga tctttaagct ttttacattt tcagctcgca tatatcaatt cgaacgttta
361 attagaatgt ttaaataaag ctagattaaa tgattaggct cagttaccgg tctttttttt
421 ctcatttacg tgcgaactct gcttaaactc tagttattct ttattaatat gtggttattt
481 ttatatatgt atgttatcat aactgtactg gctatgtcag gtggtaatga ctgtaacgtt
541 acgttactcg ttgtaggcac gacattgaat gggccggtgt tgaaataagt cttcaacccc
601 ttttaacctc aaaatgtgct ctggttaaca aggattttaa cagctatcag tatgactgtg
661 cggttttaaa gccgttagtg aggcacgttg cacacttgat ggatggccgg aatgggaagt
721 tctttatgca ggcagtgctg cagcagggtg tgacctactt tagctaacgt tagccggcta
781 accagcattc atctgccggt aacttgagtc taatattctc tatgtgatat cgaagtgatc
841 aaagacacgt ctgttagctc actttaacca actgtagtga aaaatagcgc agtgtgcagc
901 ccttcaagtc tttcatttag gctgattatt caatcatttt attaactatt aacgcgttac
961 taaacgtaag gtaacgtagt cagtttttaa taactggtga aaagtactgg ttgggtttaa
1021 atggtgactt ataattgtgt tggaggggga aacctttttg ataaaggcta tataatctca
1081 aatgaatggg ctgaggatgg tgttcacagg tgctttagtg aagtccgctc gtgaagagtc
1141 gctgaagtga ctgcagatct gtagcgcatg cgttttggca gacggccgtt gaaattcggt
1201 tgagtaattg ataccaggtg aggctagagg atgtagaaat tcatttgtgt agaatttagg
1261 gagtggcctg gcgtgatgaa tgtcgaaatc cgttcctttt tactgaaccc tatgtctctg
1321 ctgagtgcca caccgccggc acaaagcgtc tcaaaccatt gccttttatg gtaataatga
1381 gaatgcagag ggacttcctt tgtctggcac atctgaggcg cgcattgtca cactagcacc
1441 cactagcggt cagactgcag acaaacagga agctgactcc acatggtcac atgctcactg
1501 aagtgttgac ttccctgaca gctgtgcact ttctaaaccg gttttctcat tcatttacag
1561 ttcagccatg gatgatgaaa ttgccgcact ggttgttgac aacggatccg gtatgtgcaa
1621 agccggattc gctggagatg atgctccccg tgccgtcttc ccatccatcg tgggtcgccc
1681 cagacatcag gtgagaaact gaggattaat ttcaggctgc ccaataattt ggaatttttt
1741 ttttttaact tcctagttcc ttaatgttta tctaattaac acttgtcttg ttacagggtg
1801 tcatggttgg tatgggacag aaggacagct acgttggtga tgaggctcag agcaagagag
1861 gtatcctgac cctgaagtac cccatcgagc acggtattgt caccaactgg gatgacatgg
1921 agaagatctg gcatcacacc ttctacaacg agctgcgtgt cgccccagag gagcaccccg
1981 tcctgctcac agaggccccc ctgaacccca aggccaacag ggaaaagatg acacaggttg
2041 gtttttggct ggcgactggt gcttggaagt ctcttgtctg tcctgttacc tcattttaag
2101 ttctcctttt cattcacttc atccaggctt tgttctctga gctcctgagt ttcttcctct
2161 tttgctggaa ggcaggttat ctatacattg cctgcttgtt ttgcagtttt gacacaccta
2221 ttctttctgc acttatttct ttactccgga tattcaacta acctctgcat ggatttgatg
2281 gatggtgtgc tgtagctgtt tttagcataa agttaacttc tcaaacgctc tctttccaga
2341 tcatgtttga gaccttcaac acccccgcca tgtacgttgc catccaggct gtgctgtccc
2401 tgtatgcctc tggtcgtacc actggtatcg tgatggactc tggtgatggt gtcacccaca
2461 ctgtgcccat ctacgagggt tacgccctgc cccatgccat cctccgtctg gacttggctg
2521 gccgtgacct gacagactac ctcatgaaga tcctgaccga gagaggctac agcttcacca
2581 ccacagccga gagggaaatt gtccgtgaca tcaaggagaa gctctgctat gtggctcttg
2641 acttcgagca ggagatgggc actgctgctt cctcctcctc cctggagaag agctatgagc
2701 tgcctgacgg acaggtcatc accattggca atgagaggtt caggtgccca gaggccctgt
2761 tccagccatc cttcttgggt aggtatcctg acaaacactt cctggtctgt gtatgtactt
2821 ctagatttta gttggagaac atctgaagaa cttttcatct tccttttgca ggtatggagt
2881 cttgcggtat ccatgagacc accttcaact ccatcatgaa gtgtgacgtc gacatccgta
2941 aggacctgta tgccaacact gtattgtctg gtggtaccac catgtaccct ggcattgctg
3001 acaggatgca gaaagagatc acatccctgg cccccagcac aatgaaaatc aaggtgagct
3061 aggccttgag ctatgactgt tacctctcct atcagtctaa taactgaaca tttgtcagct
3121 ctgcatcttg ataatcgttc atctctctct acagatcatt gccccacctg agcgtaaata
3181 ctctgtctgg atcggaggtt ccatcctggc ctccctgtcc accttccagc agatgtggat
3241 tagcaagcag gagtatgatg agtctggacc atccatcgtc caccgcaaat gcttctaaac
3301 ggactgttac cacttcacgc cgactcaact gcgcagagaa aaacttcaaa cgacaacatt
3361 ggcatggctt ttgttatttt tggcgcttga ctcaggatct aaaaactgga acggcgaagg
3421 tgacggcaat gttttggcaa ataagcatcc ccgaagttct acaatgcatc tgaggactca
3481 atgttttttt tttttttttt tctttagtca ttccaaatgt ttgttaaatg cattgttccg
3541 aaacttattt gcctctatga aggctgccca gtaattggga gcatacttaa cattgtagta
3601 ttgtatgtaa attatgtaac aaaacaatga ctgggttttt gtactttcag ccttaatctt
3661 gggttttttt ttttttttgg ttccaaaaaa ctaagcttta ccattcaaga tgtaaaggtt
3721 tcattccccc tggcatattg aaaaagctgt gtggaacgtg gcggtgcaga catttggtgg
3781 ggccaacctg tacactgact aattcaaata aaagtgcaca tgtaagacat cctactctgt
3841 gtgatttttc tgtttgtgct gagtgaactt gctatgaagt cttttagtgc actctttaat
3901 aaaagtagtc ttcccttaaa gtgtcccttc ccttatggcc ttcacatttc tcaactagcg
3961 cttcaactag aaagcacttt agggactggg atgc
//