GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS LABORGRPL 3331 bp ds-DNA BCT 31-JAN-1991
DEFINITION Plasmid pLAB1000 (from Lactobacillus hilgardii) replication (Rep)
and mobilization (Mob) genes, complete cds.
ACCESSION M55222 M37906
KEYWORDS mobilization protein; origin of replication; replication protein.
SOURCE Plasmid pLAB1000 (from Lactobacillus hilgardii) DNA.
ORGANISM Plasmid pLAB1000
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 3331)
AUTHORS Josson,K., Soetaert,P., Michiels,F., Joos,H. and Mahillon,J.
TITLE Lactobacillus hilgardii plasmid pLAB1000 consists of two funcional
cassettes commonly found in other gram-positive organisms
JOURNAL J. Bacteriol. 172, 3089-3099 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 77..1162
/gene="mob"
/codon_start=77
/product="mobilization protein"
terminator 1700..1728
/note="potential"
/gene="mob"
terminator 1756..1777
/note="potential"
/gene="mob"
terminator 2075..2151
/note="potential"
/gene="mob"
CDS 2216..3160
/gene="rep"
/codon_start=2216
/product="replication protein"
terminator 3160..3200
/note="potential"
/gene="rep"
BASE COUNT 1109 a 562 c 739 g 921 t
ORIGIN
1 gagctcacac tttataacat aaagtatagt gggttatact ttacttggaa ggtaactccg
61 aaatgaggtg catacaatga gttttgcagt ggctagaatg acgaaattaa aagctgataa
121 tttagtcggc attgggaatc atgaccaacg gaaaacgact aatcacagca acgaagatat
181 tgatgtttcc cactctcacc tgaattatga tttagtggct gggcgcactg ataactttaa
241 aacggatatt gaagcctata tcaacgaaaa caaagcgagt aagcgggcag ttcgcaaaga
301 cgctgtttta gtcaatgagt ggattataac cagtgacaaa gatttttttg agcaattaga
361 cgaagccgaa acccgtaaat attttgaaac agccaaacaa tattttgcag ataactatgg
421 tgacgaaaat attcgctatg cagttgttca tatggacgag aagacccctc acatgcatat
481 gggcattgtg ccctttgatg atgataaaaa gctctcagct aagcgtatat tcaatcgtga
541 agccttacag cgcattcaag aggaattacc acagtacctc aaagaaaatg gctttgatgt
601 tcaacgtggt aacaaaaata aagagcgtaa gaatttatca gtacccgaat acaaagctat
661 gcgggaagaa ttgaaaaaaa tagagaccga aaaacaagag acacaagcaa agcttgcaga
721 tactaaaaaa cagcttgatg aaatcaaacc acgggatacc aaaaaaattg ctagtaaacc
781 caccttgatg aataaaaata aagtcacggt tgataaatct gatctcgctg atttggaaca
841 aagggcggtg actagcgacg cttataactt tgaaaaaatt catctggaag tagaaaatca
901 tagtttacgt aatgatttga gtgaagccaa gggccgcaac tatgaactga gaaaagaaaa
961 tgagcgattg caaaaactag taggaacgct tcaaggcatt atacgaaatg ttgatgagtt
1021 tctacacaaa aaactaggta ttaatttacc tgaaaagtgg ctagagcgtg caggactaaa
1081 agaaccgtct aaaaaagccc ctgaaagctc acaggagcgt tcagagggac accatgagga
1141 attagatggt ccaagccttt agattccttt accggttaaa aggcttgcta gatatgctat
1201 tttagctgtt attttttgtc tatttgatta cctaaaagtc ctactaatac tccacatact
1261 attaagacat atcctgttat attgtgataa ggccaattgt aatggttact tgcccataaa
1321 ataaataata aggcgatgat cagtatactt cgaatccata ggctattttt catatataac
1381 caatccttat tgccaaccga aactttcaac gactgtatta ctttctattg tttgatcgtt
1441 tgcgtgtgct tgaatagcgg gaaaactagt agctaaaagt gttgtaccaa caatagcaaa
1501 ggtaactgat ttaataattt ttatgagtat attcctcctt aaaatactta gtacaattat
1561 attttattgc taaaagtaag cgtactcaat aaaaatgttg agaggctaat gctgccgctg
1621 tcaatggtac cggacgagcg gagcgtggga ggataaggaa ttgacagcat tactcgaatc
1681 tagacactaa ccggcttgcc ggaacaagta gatttaccac ttgttccgcc tgccacacgg
1741 cgagtgggag tgagtttgag cggggaaccc cgctcaatta tagggtcaag ctggtacagc
1801 ttggactggg ttcggggcgt cagcgcccga gggcttggaa actggtcgat tttgaccagt
1861 ttcttcttat cttgatacta ctacaaacaa cgtcattttt ttggttttgc tgtaaagcgt
1921 tgacaggccg gcgtttatag tgcataattt ggcttattaa agttgactga gggttttgaa
1981 cttttattga ctcagttgtc ttagattatt taaaaaactg cataaaaaaa ctcacgtgga
2041 aatcctaact ttggcgagca ggacacgtga gctaatcaat aatatacgtg agataagtct
2101 actgacaagt gagttatttg tcaacgttga ctatctaaag tctattttta tactttacga
2161 atgtgattag ctccaagcca cgtgagaaat tcattgctgg attggagcta tttttatgtc
2221 gaaaaagatt ttaaaagatg ttagtcgtaa cagaaaagaa cggccgtggc gtgaacgtaa
2281 attggaaaac ttacagtacg cggagtattt gagaatactt aattttaaaa aagctaatcg
2341 tgttaaagaa tgcggtgaag tattgagatt tgttgctgat gatgagggcc gactgcggct
2401 ttatcagaca tggttttgta agagtcgatt gtgtccattg tgcaattggc gtcgctcaat
2461 gggacaatct aatcagttaa tgcaagtttt agacgaagct cataagcaac gaaaaacggg
2521 tcgctttttg tttttaacgc tgacggcgga aaacgcatct ggcgaaaact tgaaacagga
2581 agtcagaaaa atgggacggg caatttctaa gctgtttcaa tacaagaaac cggccaaaaa
2641 cttgttgggt tatgtgcgtt caaccgaaat tacgatcaac aaaaatggga cttatcatca
2701 gcacatgcac gtgctgcttt tcgttaaacc aacgtatttt aaagattcag caaattatat
2761 taatcaggca gagtggtcaa aactttggca acgggctatg aagttagatt atcagccaat
2821 tgttaatgtt gaggctgttc gctcgaacaa ggctaaaggt aaaaactcgt taattgcgag
2881 tgcacaagaa accgctaaat atcaagttaa gtccaaagat attttgacga atgaccaaga
2941 acgtgacctg caagtggttg aggatttgga acagggattg gcggggtcac gtcaaattag
3001 ttatggtggg ttatttaaag aaattcgcaa gcagttacaa cttgaagatg ttgatgctca
3061 cttaattaat gtcgatgatg ataaagtaaa aatcgatgag gtggttcgcg aggtcgttgc
3121 gaaatgggat tataataagc aaaattattt tatctggtaa aggggaaaag aacggagcta
3181 gtatccgttc ttttttgctt gaaaaagcaa acgtgagttg agattatttt ggcgtaaaaa
3241 aacagccaaa attttcgata acgagagcgg aatttttcgt cagaaaaata agcggtgggg
3301 tctgtcccgc tggcaaggcg tccctctgaa a
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