GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS ECOPCKA 5352 bp ds-DNA BCT 16-JAN-1991
DEFINITION E.coli phosphoenolpyruvate carboxykinase (pckA) gene, complete cds.
ACCESSION M59823 M34605
KEYWORDS pckA gene; phosphoenolpyruvate carboxykinase.
SOURCE E.coli (K12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 5352)
AUTHORS Medina,V., Pontarollo,R., Glaeske,D., Tabel,H. and Goldie,H.
TITLE Sequence of the pckA gene of Escherichia coli K-12: Relevance to
genetic and allosteric regulation and homology of E.coli
phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma
brucei and Saccharomyces cerevisiae
JOURNAL J. Bacteriol. 172, 7151-7156 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
-35_signal 101..106
/gene="pckA"
-10_signal 127..133
/gene="pckA"
mRNA 140..1673
/gene="pckA"
/partial
/product="phosphoenolpyruvate carboxykinase"
misc_binding 247..254
/note="nucleotide binding site"
RBS 267..271
/gene="pckA"
CDS 279..1673
/EC_number="4.1.1.49"
/gene="pckA"
/codon_start=279
/product="phosphoenolpyruvate carboxykinase"
misc_binding 316..328
/note="calcium binding site"
BASE COUNT 1297 a 1492 c 1355 g 1198 t 10 others
ORIGIN
1 atcgatactt taccggttga atttgcattt tcattcagga atgcgattcc actcacaata
61 ttcccgccat ataaaccaag atttaacctt ttgagaacat tttccacacc taaaatgcta
121 tttctgcgat aatagcaacc gtttcgtgac aggaatcacg gagttttttg tcaaatatga
181 atttctccag atacgtaaat ctatgagcgt tgtcgcggtt aacaccccca aaaagacttt
241 actattcagg caatacatat tggctaagga gcagtgaaat gcgcgttaac aatggtttga
301 ccccgcaaga actcgaggct tatggtatca gtgacgtaca tgatatcgtt tacaacccaa
361 gctacgacct gctgtatcag gaagagctcg atccgagcct gacaggttat gagcgcgggg
421 tgttaactaa tctgggtgcc gttgccgtcg ataccgggat cttcaccggt cgttcacaaa
481 agataagtat atccgtccgt gacgatacca ctcgcgatac tttctggtgg gcagacaaag
541 gcaaaggtaa gaacgacaac aaacctctct ctccggaaac ctggcagcat ctgaaaggcc
601 tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt cgacgctttc tgtggtgcga
661 acccggatac tcgtctttcc gtccgtttca tcaccgaagt ggcctggcag gcgcattttg
721 tcaaaaacat gtttattcgc ccgagcgatg aagaactggc aggtttcaaa ccagacttta
781 tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa agaacagggt ctcaactccg
841 aaaacttcgt gcgtttaacc gaccgagcgc atgcagctga ttgcggcacc tggtacggcg
901 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
961 cttctatgca ctcgtccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc
1021 tttccggcaa cggtaaaaca gcctttccac gaccgaaacg tcgcctgatt ggcgatgacg
1081 aacacggctg ggacgatgac ggcgtgttta acttgaaggc ggctgggtac gcaaaaacta
1141 tcaagctgtc gaaagaagcg gaacctgaaa tctacaagct atccgtcgtg atggcgttgc
1201 tggaaaacgt caccgtgcgt gaagatggca ctatcgactt tgatgatggt tcaaaaaccg
1261 agaacacccg cgtttcttat ccgatctatc acatcgataa cattgttagg ccggtttcca
1321 aagcgggcca cggacgaagg ttatcattgc tgactgctca tgctttcggc gtgttgccgc
1381 cggtttctcg cctgactgcc gatcaaaccc agtatcactt cctctctggc ttcaccgcga
1441 aactggccgg tactgagcgt ggcatcaccg aaccgacgcc aaccttctcc gcttgcttcg
1501 gcgcggcatt cctgtcgctg cacccgactc agtacgcaga agtgctggtg aaacgtatgc
1561 aggcggcggg cgcgcaggct tatctggtta acactggctg gaacggcagt ggcaaaagta
1621 tctcgattaa agataccgcg cattatcgac gcatctcacg gttcgctgga taatcgaaac
1681 tttaactgtt acctgcatcg caatcgaatc cgggcgtaga agcgatcaat tctcgatccg
1741 cgtaacacta cgcttatcgg tcatgcagaa acgaactgcg aactctttat cgaattgtag
1801 taacgaacct cgtcgtcgta cctgtcaatt atatcatcga atatcaacta gcagttaagg
1861 ccacgaccga aacgtcgcct gattggcgat gacgaacacg gctgggacga tgacggcgtc
1921 cgacccgaaa cgtcgtctga ttggcgatga tgagcacggt tgggatgacg acggcgtngt
1981 tcaattttga aggcggctgn ttgggatgac gacggcnggc tgctacgcca aaaccatcaa
2041 actgtcgaaa gaagcggagc cagaaattta ntcgtctgat tggcgatgat gntgatgcgc
2101 tgctggaaaa cgtcaccgtg cgcgaagacg gcacgacttt gatgatggtt caaaaaccga
2161 gaacacccgc gtttcttatc cgatctatca catcgatttc gacgatggtt ccaaaaccga
2221 aaacacccgc gtctcttacc cgatctatca tatcngataa catcgttaag cccgtgtcca
2281 aagcaggtca cgntctatca tatcgataac atcgttaant tcggcgtgtt gccgccggtt
2341 tcccgcaaac ccagtatcac ttcctctctg gcttcaccgc gaaactggcc ggtactgagc
2401 gtggccagac gcagtaccac ttcctgtcag gtttcaccgc taaactggcc ggcaccgaac
2461 gcggcatcac cgaaccgacg ccaaccttct ccgcttgctt cggcgcggca ttcctgtcgc
2521 tgcacgtcac cgaaccgacc cccaccttct ccgcctgctt cggcgccgcg ttcctgacgc
2581 tgcacccgac tcagtacgca gaagtgctgg tgaaacgtat gcaggcggcg ggcgcgcagg
2641 cttatccaac gcagtatgct gaggtgctgg tgaaacgtat gcaggcagca ggcgcgcagg
2701 cttatctggt taacactggc tggaacggca gtggcaaaag tatctcgatt aaagataccg
2761 cgcctggtta acaccggctg gaatggcacc ggcaaacgta tctccatcaa agatacgcgc
2821 gcattatcga cgcnaattat ccgacgcntc tgaacggttc tcttgataag gtaaaacagc
2881 ctttccacga ccgaaacgtc gcctgattgg cgatgacgaa cacaccggaa aaaccactct
2941 ctccaccgac ccgaaacgtc gtctgattgg cgatgatgag cacggctggg acgatgacgg
3001 cgtgtttaac ttgaaggcgg ctgggtacgc aaaaactatc aaggttggga tgacgacggc
3061 gtgttcaatt ttgaaggcgg ctgctacgcc aaaaccatca agctgtcgaa agaagcggaa
3121 cctgaaatct acaagctatc cgtcgtgatg gcgttgctgg actgtcgaaa gaagcggagc
3181 cagaaattta tcatgcgatt cgccgtgatg cgctgctgga aaacgtcacc gtgcgtgaag
3241 atggcactat cgactttgat gatggttcaa aaaccgagaa aaacgtcacc gtgcgcgaag
3301 acggcaccgt tgatttcgac gatggttcca aaaccgaaaa cacccgcgtt tcttatccga
3361 tctatcacat cgataacatt gttaggccgg tttccaaaga cacccgcgtc tcttacccga
3421 tctatcatat cgataacatc gttaagcccg tgtccaaagc gggccacgga cgaaggttat
3481 cattgctgac tgctcatgct ttcggcgtgt tgccgccgca ggtcacgcca ccaaggttat
3541 cttcctgacg gcggaccgtt cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa
3601 acccagtatc acttcctctc tggcttcacc gcgaaagttt cccgtttaac cgctaaccag
3661 acgcagtacc acttcctgtc aggtttcacc gctaaactgg ccggtactga gcgtggcatc
3721 accgaaccga cgccaacctt ctccgcttgc ttcggcctgg ccggcaccga acgcggcgtc
3781 accgaaccga cccccacctt ctccgcctgc ttcggcgcgg cattcctgtc gctgcacccg
3841 actcagtacg cagaagtgct ggtgaaacgt atgcaggccg cgttcctgac gctgcaccca
3901 acgcagtatg ctgaggtgct ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg
3961 gttaacactg gctggaacgg cagtggcaaa agtatcgcag caggcgcgca ggcttatctg
4021 gttaacaccg gctggaatgg caccggcaaa cgtatctcga ttaaagatac cgcgcattat
4081 cgacgcatct cacggttcgc tggataatct ccatcaaaga tacgcgcgca attatccgac
4141 gccattctga acggttctct tgataacgcg aaactttaac tgttacctgc atcgcaatcg
4201 aatccgggcg tagaagcgat caattctgga aaccttccgt ctgccgctgt tcgatctggc
4261 gatcccgatc cgcgtaacac tacgcttatc ggtcatgcag aaacgaactg cgaactcttt
4321 atcgaattgt agtaacgaac ctcgtcgtcg tacctgtcaa ttatatcatc gaatatcaac
4381 tagcagttaa ggcggaaaaa ccactctctc caccgacccg aaacgtcgtc tgattggcga
4441 tgatgagcac ggttgggatg acgacggcgt gttcaatttt gaaggcggct gctacgccaa
4501 aaccatcaaa ctgtcgaaag aagcggagcc agaaatttat catgcgattc gccgtgatgc
4561 gctgctggaa aacgtcaccg tgcgcgaaga cggcaccgtt gatttcgacg atggttccaa
4621 aaccgaaaac acccgcgtct cttacccgat ctatcatatc gataacatcg ttaagcccgt
4681 gtccaaagca ggtcacgcca ccaaggttat cttcctgacg gcggaccgtt cggcgtgttg
4741 ccgccggttt cccgtttaac cgctaaccag acgcagtacc acttcctgtc aggtttcacc
4801 gctaaactgg ccggcaccga acgcggcgtc accgaaccga cccccacctt ctccgcctgc
4861 ttcggcgccg cgttcctgac gctgcaccca acgcagtatg ctgaggtgct ggtgaaacgt
4921 atgcaggcag caggcgcgca ggcttatctg gttaacaccg gctggaatgg caccggcaaa
4981 cgtatctcca tcaaagatac gcgcgcaatt atccgacgcc attctgaacg gttctcttga
5041 taacgcggaa accttccgtc tgccgctgtt cgatctggcg atcccgactg aactgccagg
5101 cgtggatacc cacattctcg acccgcgtaa cacctacgcg tcgccggaac aatggcagga
5161 aaaagccacc gcgctggcga aactgttcat tgagaacttc gagaagtata cggatacccc
5221 ggcaggcgaa cgctggttag cgccggaccg aagctgtaag gaacaggccg gatggcggct
5281 ttgaacggcg tcatccggcg ttgagaagaa agggagggta atacctccct ttcttatgcc
5341 tcttttgtcg tc
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS ECOPCKA 1860 bp ds-DNA BCT 31-JAN-1991
DEFINITION E.coli phosphoenolpyruvate carboxykinase (pckA) gene, complete cds.
ACCESSION M59823 M34605
KEYWORDS pckA gene; phosphoenolpyruvate carboxykinase.
SOURCE E.coli (K12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1860)
AUTHORS Medina,V., Pontarollo,R., Glaeske,D., Tabel,H. and Goldie,H.
TITLE Sequence of the pckA gene of Escherichia coli K-12: Relevance to
genetic and allosteric regulation and homology of E.coli
phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma
brucei and Saccharomyces cerevisiae
JOURNAL J. Bacteriol. 172, 7151-7156 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
-35_signal 101..106
/gene="pckA"
-10_signal 127..133
/gene="pckA"
mRNA 140..1673
/gene="pckA"
/partial
/product="phosphoenolpyruvate carboxykinase"
misc_binding 247..254
/note="nucleotide binding site"
RBS 267..271
/gene="pckA"
CDS 279..1673
/EC_number="4.1.1.49"
/gene="pckA"
/codon_start=279
/product="phosphoenolpyruvate carboxykinase"
misc_binding 316..328
/note="calcium binding site"
BASE COUNT 476 a 474 c 455 g 455 t
ORIGIN
1 atcgatactt taccggttga atttgcattt tcattcagga atgcgattcc actcacaata
61 ttcccgccat ataaaccaag atttaacctt ttgagaacat tttccacacc taaaatgcta
121 tttctgcgat aatagcaacc gtttcgtgac aggaatcacg gagttttttg tcaaatatga
181 atttctccag atacgtaaat ctatgagcgt tgtcgcggtt aacaccccca aaaagacttt
241 actattcagg caatacatat tggctaagga gcagtgaaat gcgcgttaac aatggtttga
301 ccccgcaaga actcgaggct tatggtatca gtgacgtaca tgatatcgtt tacaacccaa
361 gctacgacct gctgtatcag gaagagctcg atccgagcct gacaggttat gagcgcgggg
421 tgttaactaa tctgggtgcc gttgccgtcg ataccgggat cttcaccggt cgttcacaaa
481 agataagtat atccgtccgt gacgatacca ctcgcgatac tttctggtgg gcagacaaag
541 gcaaaggtaa gaacgacaac aaacctctct ctccggaaac ctggcagcat ctgaaaggcc
601 tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt cgacgctttc tgtggtgcga
661 acccggatac tcgtctttcc gtccgtttca tcaccgaagt ggcctggcag gcgcattttg
721 tcaaaaacat gtttattcgc ccgagcgatg aagaactggc aggtttcaaa ccagacttta
781 tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa agaacagggt ctcaactccg
841 aaaacttcgt gcgtttaacc gaccgagcgc atgcagctga ttgcggcacc tggtacggcg
901 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
961 cttctatgca ctcgtccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc
1021 tttccggcaa cggtaaaaca gcctttccac gaccgaaacg tcgcctgatt ggcgatgacg
1081 aacacggctg ggacgatgac ggcgtgttta acttgaaggc ggctgggtac gcaaaaacta
1141 tcaagctgtc gaaagaagcg gaacctgaaa tctacaagct atccgtcgtg atggcgttgc
1201 tggaaaacgt caccgtgcgt gaagatggca ctatcgactt tgatgatggt tcaaaaaccg
1261 agaacacccg cgtttcttat ccgatctatc acatcgataa cattgttagg ccggtttcca
1321 aagcgggcca cggacgaagg ttatcattgc tgactgctca tgctttcggc gtgttgccgc
1381 cggtttctcg cctgactgcc gatcaaaccc agtatcactt cctctctggc ttcaccgcga
1441 aactggccgg tactgagcgt ggcatcaccg aaccgacgcc aaccttctcc gcttgcttcg
1501 gcgcggcatt cctgtcgctg cacccgactc agtacgcaga agtgctggtg aaacgtatgc
1561 aggcggcggg cgcgcaggct tatctggtta acactggctg gaacggcagt ggcaaaagta
1621 tctcgattaa agataccgcg cattatcgac gcatctcacg gttcgctgga taatcgaaac
1681 tttaactgtt acctgcatcg caatcgaatc cgggcgtaga agcgatcaat tctcgatccg
1741 cgtaacacta cgcttatcgg tcatgcagaa acgaactgcg aactctttat cgaattgtag
1801 taacgaacct cgtcgtcgta cctgtcaatt atatcatcga atatcaacta gcagttaagg
//