GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS ECOPROT 4248 bp ds-DNA BCT 31-JAN-1991
DEFINITION E.coli ATP-dependent protease binding subunit (clpB) gene, complete
cds, and 16S rRNA gene, 5' end.
ACCESSION M29364 J01699
KEYWORDS 16S ribosomal RNA; ATP-binding protein; ATP-dependent protease;
ATP-protease.
SOURCE E.coli (strain K-12), clone pLC[23,30].
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 3131 to 4248)
AUTHORS Shen,W.-F., Squires,C. and Squires,C.L.
TITLE Nucleotide sequence of the rrnG ribosomal RNA promoter region of
Escherichia coli
JOURNAL Nucleic Acids Res. 10, 3303-3313 (1982)
STANDARD simple staff_review
REFERENCE 2 (bases 1 to 3130)
AUTHORS Squires,C., Ross,B.M. and Squires,C.L.
TITLE The clpB gene of E.coli specifies an ATP-dependent protease subunit
JOURNAL Unpublished (1989) 701 Fairchild Bldg. Columbia U., NY NY 10027
STANDARD simple staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1989)
701 Fairchild Bldg. Columbia U., NY NY 10027] kindly submitted
by C.L.Squires, 23-OCT-1989.
FEATURES Location/Qualifiers
CDS <1..427
/note="unidentified reading frame 1 (AA at 2)"
misc_signal 491..495
/note="-35 region (put.)"
misc_signal 514..520
/note="-10 region (put.)"
mRNA 525..3160
/note="clpB mRNA"
CDS 557..3130
/note="ATP-dependent protease binding subunit"
misc_signal 3133..3159
/note="ORF-BG transcription terminator"
rRNA 3572..>4248
/note="16S rRNA (rrnG)"
BASE COUNT 1098 a 965 c 1201 g 984 t
ORIGIN 57 minutes on the K12 map
1 agatgactgc cgactgcctc cctgtgctgt ttttgcaatc gacgggaacg aagtcgccgc
61 cgctcatgct ggctggcgtg gactgtgcgc aggcgtgctg gaagagacgg tttcctgttt
121 tgctgataat ccggaaaata ttctcgcctg gttagggccg gcaattggtc cacgcgcgtt
181 cgaagtgggg ggggaggttc gcgaggcgtt tatggcagta gacgctaaag caagtgcagc
241 tttcattcag catggtgata agtatctggc ggatatttat cagcttgccc ggcagcgtct
301 ggcgaacgtg ggtgttgagc aaattttcgg cggcgaccgt tgtacatata cggaaaatga
361 gactttcttc tcttatcgtc gcgacaagac caccggtcgt atggcaagtt tcatttggct
421 gatataacct aaagaatcaa gacgatccgg tacgcgtgat tttcttttca cattaatctg
481 gtcaataacc ttgaataatt gagggatgac ctcatttaat ctccagtagc aactttgatc
541 cgttatggga ggagttatgc gtctggatcg tcttactaat aaattccagc ttgctcttgc
601 cgatgcccaa tcacttgcac tcgggcacga caaccaattt atcgaaccac ttcatttaat
661 gagcgccctg ctgaatcagg aagggggttc ggttagtcct ttattaacat ccgctggcat
721 aaatgctggc cagttgcgca cagatatcaa tcaggcatta aatcgtttac cgcaggttga
781 aggtactggt ggtgatgtcc agccatcaca ggatctggtg cgcgttctta atctttgcga
841 caacgtggcg caaaaacgtg gtgataactt tatctcgtca gaactgttcg ttctggcggc
901 acttgagtct cgcggcaccg tggccgacat cctgaaagca gcaggggcga ccaccgccaa
961 cattactcaa gcgattgaac aaatgcgtgg aggtgaaagc gtgaacgatc aaggtgctga
1021 agaccaacgt caggctttga aaaaatatac catcgacctt accgaacgag ccgaacaggg
1081 caaactcgat ccggtgattg gtcgtgatga agaaattcgc cgtaccattc aggtgctgca
1141 acgtcgtact aaaaataacc cggtactgat tggtgaaccc ggcgtcggta aaactgccat
1201 cgttgaaggt ctggcgcagc gtattatcaa cggcgaagtg ccggaagggt tgaaaggccg
1261 ccgggtactg gcgctggata tgggcgcgct ggtggctggg gcgaaatatc gcggtgagtt
1321 tgaagaacgt ttaaaaggcg tgcttaacga tcttgccaaa caggaaggca acgtcatcct
1381 atttatcgac gaattacata ccatggtcgg cgcgggtaaa gccgatggcg caatggacgc
1441 cggaaacatg ctgaaaccgg cgctggcgcg tggtgaattg cactgcgtag gtgccacgac
1501 gcttgacgaa tatcgccagt acattgaaaa agatgctgcg ctggaacgtc gtttccagaa
1561 agtgtttgtt gccgagcctt ctgttgaaga taccattgcg attctgcgtg gcctgaaaga
1621 acgttacgaa ttgcaccacc atgtgcaaat tactgacccg gcaattgttg cagcggcgac
1681 gttgtctcat cgctacattg ctgaccgtca gctgccggat aaagccatcg acctgatcga
1741 tgaagcagca tccagcattc gtatgcagat tgactcaaaa ccagaagaac tcgaccgact
1801 cgatcgtcgt atcatccagc tcaaactgga acaacaggcg ttaatgaaag agtctgatga
1861 agccagtaaa aaacgtctgg atatgctcaa cgaagaactg agcgacaaag aacgtcagta
1921 ctccgagtta gaagaagagt ggaaagcaga gaaggcatcg ctttctggta cgcagaccat
1981 taaagcggaa ctggaacagg cgaaaatcgc tattgaacag gctcgccgtg tgggggacct
2041 ggcgcggatg tctgaactgc aatacggcaa aatcccggaa ctggaaaagc aactggaagc
2101 cgcaacgcag ctcgaaggca aaactatgcg tctgttgcgt aataaagtga ccgacgccga
2161 aattgctgaa gtgctggcgc gttggacggg gattccggtt tctcgcatga tggaaagcga
2221 gcgcgaaaaa ctgctgcgta tggagcaaga actgcaccat cgcgtaattg gtcagaacga
2281 agcggttgat gcggtatcta acgctattcg tcgtagccgt gcggggctgg cggatccaaa
2341 tcgcccgatt ggttcattcc tgttcctcgg cccaactggt gtggggaaaa cagagctttg
2401 taaggcgctg gcgaacttta tgtttgatag cgacgaggcg atggtccgta tcgatatgtc
2461 cgagtttatg gagaaacact cggtgtctcg tttggttggt gcgcctccgg gatatgtcgg
2521 ttatgaagaa ggtggctacc tgaccgaagc ggtgcgtcgt cgtccgtatt ccgtcatcct
2581 gctggatgaa gtggaaaaag cgcatccgga tgtcttcaac attctgttgc aggtactgga
2641 tgatgggcgt ctgactgacg ggcaagggag aacggtcgac ttccgtaata cggtcgtcat
2701 tatgacctct aacctcggtt ccgatctgat tcaggaacgc ttcggtgaac tggattatgc
2761 gcacatgaaa gagctggtgc tcggtgtggt aagccataac ttccgtccgg aattcattaa
2821 ccgtatcgat gaagtggtgg tcttccatcc gctgggtgaa cagcacattg cctcgattgc
2881 gcagattcag ttgaaacgtc tgtacaaacg tctggaagaa cgtggttatg aaatccacat
2941 ttctgacgag gcgctgaaac tgctgagcga gaacggttac gatccggtct atggtgcacg
3001 tcctctgaaa cgtgcaattc agcagcagat cgaaaacccg ctggcacagc aaatactgtc
3061 tggtgaattg gttccgggta aagtgattcg cctggaagtt aatgaagacc ggattgtcgc
3121 cgtccagtaa atgataaaac gagcccttcg gggctcgttt ttgtctataa gttagacgga
3181 aaagactata tttaagatgt tttgcctgaa aagtgagcga acgataaagt ttttatattt
3241 ttcgcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
3301 aaacacgccg ccgggtcggc ggggttctcc tgagaatctc aacagagaaa agcaaagaaa
3361 tgcttgactc tgtagcggga aggcgtatta tgcacaccgc cgcgccgctg agaaaaagcg
3421 aagcggcact gctctttaac aatttatcag acaatctgtg tgggcactcg aagatacgga
3481 ttcttaacgt cgcaagacga aaaatgaata ccaagtctca agagtgaaca cgtaattcat
3541 tacgaagttt aattctttga gcatcaaact tttaaattga agagtttgat catggctcag
3601 attgaacgct ggcggcaggc ctaacacatg caagtcgaac ggtaacagga agcagcttgc
3661 tcttcgctga cgagtggcgg acgggtgagt aatgtctggg aagctgcctg atggaggggg
3721 ataactactg gaaacggtag ctaataccgc ataatgtcgc aagaccaaag agggggacct
3781 tcgggcctct tgccatcgga tgtgcccaga tgggattagc ttgttggtgg ggtaacggct
3841 cacctaggcg acgatcccta gctggtctga gaggatgacc agccacactg gaactgagac
3901 acggtccaga ctcctacggg aggcagcagt ggggaatatt gcacaatggg cgcaagcctg
3961 atgcagccat gccgcgtgta tgaagaaggc cttcgggttg taaagtactt tcagcgggga
4021 ggaagggagt aaagttaata cctttgctca ttgacgttac ccgcagaaga agcaccggct
4081 aactccgtgc cagcagccgc ggtaatacgg agggtgcaag cgttaatcgg aattactggg
4141 cgtaaagcgc acgcaggcgg tttgttaagt cagatgtgaa atccccgggc tcaacctggg
4201 aactgcatct gatactggca agcttgagtc tcgtagaggg gggtagaa
//GenBank-Updates@genbank.bio.net (02/01/91)
LOCUS ECOPROT 4248 bp ds-DNA BCT 01-FEB-1991
DEFINITION E.coli ATP-dependent protease binding subunit (clpB) gene, complete
cds, and 16S rRNA gene, 5' end.
ACCESSION M29364 J01699 V00350
KEYWORDS 16S ribosomal RNA; ATP-binding protein; ATP-dependent protease;
ATP-protease.
SOURCE E.coli (strain K-12), clone pLC[23,30].
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 3131 to 4248)
AUTHORS Shen,W.-F., Squires,C. and Squires,C.L.
TITLE Nucleotide sequence of the rrnG ribosomal RNA promoter region of
Escherichia coli
JOURNAL Nucleic Acids Res. 10, 3303-3313 (1982)
STANDARD simple staff_review
REFERENCE 2 (bases 1 to 3130)
AUTHORS Squires,C., Ross,B.M. and Squires,C.L.
TITLE The clpB gene of E.coli specifies an ATP-dependent protease subunit
JOURNAL Unpublished (1989) 701 Fairchild Bldg. Columbia U., NY NY 10027
STANDARD simple staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1989)
701 Fairchild Bldg. Columbia U., NY NY 10027] kindly submitted
by C.L.Squires, 23-OCT-1989.
FEATURES Location/Qualifiers
CDS <1..427
/note="unidentified reading frame 1 (AA at 2)"
misc_signal 491..495
/note="-35 region (put.)"
misc_signal 514..520
/note="-10 region (put.)"
mRNA 525..3160
/note="clpB mRNA"
CDS 557..3130
/note="ATP-dependent protease binding subunit"
misc_signal 3133..3159
/note="ORF-BG transcription terminator"
rRNA 3572..>4248
/note="16S rRNA (rrnG)"
BASE COUNT 1098 a 965 c 1201 g 984 t
ORIGIN 57 minutes on the K12 map
1 agatgactgc cgactgcctc cctgtgctgt ttttgcaatc gacgggaacg aagtcgccgc
61 cgctcatgct ggctggcgtg gactgtgcgc aggcgtgctg gaagagacgg tttcctgttt
121 tgctgataat ccggaaaata ttctcgcctg gttagggccg gcaattggtc cacgcgcgtt
181 cgaagtgggg ggggaggttc gcgaggcgtt tatggcagta gacgctaaag caagtgcagc
241 tttcattcag catggtgata agtatctggc ggatatttat cagcttgccc ggcagcgtct
301 ggcgaacgtg ggtgttgagc aaattttcgg cggcgaccgt tgtacatata cggaaaatga
361 gactttcttc tcttatcgtc gcgacaagac caccggtcgt atggcaagtt tcatttggct
421 gatataacct aaagaatcaa gacgatccgg tacgcgtgat tttcttttca cattaatctg
481 gtcaataacc ttgaataatt gagggatgac ctcatttaat ctccagtagc aactttgatc
541 cgttatggga ggagttatgc gtctggatcg tcttactaat aaattccagc ttgctcttgc
601 cgatgcccaa tcacttgcac tcgggcacga caaccaattt atcgaaccac ttcatttaat
661 gagcgccctg ctgaatcagg aagggggttc ggttagtcct ttattaacat ccgctggcat
721 aaatgctggc cagttgcgca cagatatcaa tcaggcatta aatcgtttac cgcaggttga
781 aggtactggt ggtgatgtcc agccatcaca ggatctggtg cgcgttctta atctttgcga
841 caacgtggcg caaaaacgtg gtgataactt tatctcgtca gaactgttcg ttctggcggc
901 acttgagtct cgcggcaccg tggccgacat cctgaaagca gcaggggcga ccaccgccaa
961 cattactcaa gcgattgaac aaatgcgtgg aggtgaaagc gtgaacgatc aaggtgctga
1021 agaccaacgt caggctttga aaaaatatac catcgacctt accgaacgag ccgaacaggg
1081 caaactcgat ccggtgattg gtcgtgatga agaaattcgc cgtaccattc aggtgctgca
1141 acgtcgtact aaaaataacc cggtactgat tggtgaaccc ggcgtcggta aaactgccat
1201 cgttgaaggt ctggcgcagc gtattatcaa cggcgaagtg ccggaagggt tgaaaggccg
1261 ccgggtactg gcgctggata tgggcgcgct ggtggctggg gcgaaatatc gcggtgagtt
1321 tgaagaacgt ttaaaaggcg tgcttaacga tcttgccaaa caggaaggca acgtcatcct
1381 atttatcgac gaattacata ccatggtcgg cgcgggtaaa gccgatggcg caatggacgc
1441 cggaaacatg ctgaaaccgg cgctggcgcg tggtgaattg cactgcgtag gtgccacgac
1501 gcttgacgaa tatcgccagt acattgaaaa agatgctgcg ctggaacgtc gtttccagaa
1561 agtgtttgtt gccgagcctt ctgttgaaga taccattgcg attctgcgtg gcctgaaaga
1621 acgttacgaa ttgcaccacc atgtgcaaat tactgacccg gcaattgttg cagcggcgac
1681 gttgtctcat cgctacattg ctgaccgtca gctgccggat aaagccatcg acctgatcga
1741 tgaagcagca tccagcattc gtatgcagat tgactcaaaa ccagaagaac tcgaccgact
1801 cgatcgtcgt atcatccagc tcaaactgga acaacaggcg ttaatgaaag agtctgatga
1861 agccagtaaa aaacgtctgg atatgctcaa cgaagaactg agcgacaaag aacgtcagta
1921 ctccgagtta gaagaagagt ggaaagcaga gaaggcatcg ctttctggta cgcagaccat
1981 taaagcggaa ctggaacagg cgaaaatcgc tattgaacag gctcgccgtg tgggggacct
2041 ggcgcggatg tctgaactgc aatacggcaa aatcccggaa ctggaaaagc aactggaagc
2101 cgcaacgcag ctcgaaggca aaactatgcg tctgttgcgt aataaagtga ccgacgccga
2161 aattgctgaa gtgctggcgc gttggacggg gattccggtt tctcgcatga tggaaagcga
2221 gcgcgaaaaa ctgctgcgta tggagcaaga actgcaccat cgcgtaattg gtcagaacga
2281 agcggttgat gcggtatcta acgctattcg tcgtagccgt gcggggctgg cggatccaaa
2341 tcgcccgatt ggttcattcc tgttcctcgg cccaactggt gtggggaaaa cagagctttg
2401 taaggcgctg gcgaacttta tgtttgatag cgacgaggcg atggtccgta tcgatatgtc
2461 cgagtttatg gagaaacact cggtgtctcg tttggttggt gcgcctccgg gatatgtcgg
2521 ttatgaagaa ggtggctacc tgaccgaagc ggtgcgtcgt cgtccgtatt ccgtcatcct
2581 gctggatgaa gtggaaaaag cgcatccgga tgtcttcaac attctgttgc aggtactgga
2641 tgatgggcgt ctgactgacg ggcaagggag aacggtcgac ttccgtaata cggtcgtcat
2701 tatgacctct aacctcggtt ccgatctgat tcaggaacgc ttcggtgaac tggattatgc
2761 gcacatgaaa gagctggtgc tcggtgtggt aagccataac ttccgtccgg aattcattaa
2821 ccgtatcgat gaagtggtgg tcttccatcc gctgggtgaa cagcacattg cctcgattgc
2881 gcagattcag ttgaaacgtc tgtacaaacg tctggaagaa cgtggttatg aaatccacat
2941 ttctgacgag gcgctgaaac tgctgagcga gaacggttac gatccggtct atggtgcacg
3001 tcctctgaaa cgtgcaattc agcagcagat cgaaaacccg ctggcacagc aaatactgtc
3061 tggtgaattg gttccgggta aagtgattcg cctggaagtt aatgaagacc ggattgtcgc
3121 cgtccagtaa atgataaaac gagcccttcg gggctcgttt ttgtctataa gttagacgga
3181 aaagactata tttaagatgt tttgcctgaa aagtgagcga acgataaagt ttttatattt
3241 ttcgcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
3301 aaacacgccg ccgggtcggc ggggttctcc tgagaatctc aacagagaaa agcaaagaaa
3361 tgcttgactc tgtagcggga aggcgtatta tgcacaccgc cgcgccgctg agaaaaagcg
3421 aagcggcact gctctttaac aatttatcag acaatctgtg tgggcactcg aagatacgga
3481 ttcttaacgt cgcaagacga aaaatgaata ccaagtctca agagtgaaca cgtaattcat
3541 tacgaagttt aattctttga gcatcaaact tttaaattga agagtttgat catggctcag
3601 attgaacgct ggcggcaggc ctaacacatg caagtcgaac ggtaacagga agcagcttgc
3661 tcttcgctga cgagtggcgg acgggtgagt aatgtctggg aagctgcctg atggaggggg
3721 ataactactg gaaacggtag ctaataccgc ataatgtcgc aagaccaaag agggggacct
3781 tcgggcctct tgccatcgga tgtgcccaga tgggattagc ttgttggtgg ggtaacggct
3841 cacctaggcg acgatcccta gctggtctga gaggatgacc agccacactg gaactgagac
3901 acggtccaga ctcctacggg aggcagcagt ggggaatatt gcacaatggg cgcaagcctg
3961 atgcagccat gccgcgtgta tgaagaaggc cttcgggttg taaagtactt tcagcgggga
4021 ggaagggagt aaagttaata cctttgctca ttgacgttac ccgcagaaga agcaccggct
4081 aactccgtgc cagcagccgc ggtaatacgg agggtgcaag cgttaatcgg aattactggg
4141 cgtaaagcgc acgcaggcgg tttgttaagt cagatgtgaa atccccgggc tcaacctggg
4201 aactgcatct gatactggca agcttgagtc tcgtagaggg gggtagaa
//