GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS SPAFLAA 1543 bp ds-DNA BCT 16-JAN-1991
DEFINITION S.aurantia flagellar filament surface antigen (flaA) gene, complete
cds.
ACCESSION M24459
KEYWORDS flagellar filament antigen.
SOURCE S.aurantia (strain M1) DNA, clone pBB701.
ORGANISM Spirochaeta aurantia
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Spirochetes;
Spirochaetales; Spirochaetaceae.
REFERENCE 1 (sites)
AUTHORS Brahamsha,B. and Greenberg,E.P.
TITLE Cloning and sequence analysis of flaA, a gene encoding a
Spirochaeta aurantia flagellar filament surface antigen
JOURNAL J. Bacteriol. 171, 1692-1697 (1989)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1543)
AUTHORS Greenberg,E.P.
TITLE ;
JOURNAL Unpublished (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol. 171,
1692-1697 (1989)] kindly provided
by E.P.Greenberg, 26-APR-1989.
FEATURES Location/Qualifiers
CDS 209..1222
/note="flagellar filament surface antigen precursor"
/codon_start=209
sig_peptide 209..271
/note="put. flagellar filament surface antigen signal
peptide"
/codon_start=209
mat_peptide 272..1219
/note="put. flagellar filament surface antigen"
/codon_start=272
BASE COUNT 330 a 504 c 403 g 306 t
ORIGIN 73 bp upstream of NruI site.
1 aaaagttcct tgattcccga ccccgatgtt ctatattctc ccgtaaaggc cctcgagatt
61 ctggagacca ccgtgatggc gaactttccg ggaatctcga aggtgagggt gtcggtggcg
121 gagagccgcc ggggatttgt cgttgacaaa ggatcaagca tccatataat gaatttcagc
181 agaatccaag gtaaataagg agcactggat gaaaaggttc ttcgcgattc tgggggctgc
241 cctgttcgtg ggtaacagtg gtgccttcgc agaacaagct actctcattg atttctctaa
301 gttggttggc gaaggtaaca ccgggttgca cgctccgacg acgatcgact acagccgcca
361 ggctgggtcg gcctacagtg ccgaagataa ggcagcgatg aagatttctc tggccattcc
421 cagctgggag atcgagttgg cttcttcgtc ccagaccgtc gagaaccaga ctctttcgtt
481 ggtgaccgcc gctcccgtga agcaggacgc cgcccgctac ggtggtgaaa ccgtcatggg
541 tgttcggatt cacttcccct cgttcggcat caactccttt gccgtcatca agcctccgtt
601 caccatcccc gcctacgcca ccctcggcga cgccacggcc cagaacgcgg tggccggtgg
661 tcagttcgac ggcttcggcg tcctcaagaa cgtcggcgtc atcaagtcca tccagatcaa
721 cattctcgga cgcaactacc tgaaccgcct gtccctcctc ctcgaggacc agaacggcga
781 tgagcgcgag atcgtcatgg gctacctgaa cttcgacggt tggaagtcgc tccagtggaa
841 caaccccaac taccagaccg aagtccgcaa ccgcgacctt cagatcgtgc ccctctaccc
901 ccggtcggct cccctcatca agctcaaggg catcaagatc caccgcgacg gttcccagga
961 aggcggcgac atcgtctcct acatcaagga catcaaggtg atctacgacc aggccgtggt
1021 tgaccgcaac agcgacgtcg acgacgaggc catctggggc atcctccgcc agcgcgaaga
1081 gcagtaccgc aacttcgaac tcgccaagct cggtaacctc caggttctcc gcagcctcga
1141 gaagaagaag atggccaagg aagctgattt cgaccaggcc gctcccgccg ctgccgctgc
1201 tcgggccccc gctacgaact aatctcggat ctccgaagaa gccggctctc ttgggagccg
1261 gctttttttt tcaccacgac ttgaggaaag cccccatgaa cagccccgca aaccccagcc
1321 tcgcctccac cgttgcccag accctaggcc tgcccggtcc tgcggtgacc acggtcttcg
1381 agcttctgga ccaagaccac accatcccct tcatcgcccg gtacccggat cctctagagt
1441 cgacctgcag gcatcgaagc ttggcactgg ccgacgtttt acaacgtcgt gatctgggaa
1501 aaccctggcg ttacccaact taatcgcctt gcagcacatc ccc
//GenBank-Updates@genbank.bio.net (02/01/91)
LOCUS SPAFLAA 1425 bp ds-DNA BCT 01-FEB-1991
DEFINITION S.aurantia flagellar filament surface antigen (flaA) gene, complete
cds.
ACCESSION M24459
KEYWORDS flagellar filament antigen.
SOURCE S.aurantia (strain M1) DNA, clone pBB701.
ORGANISM Spirochaeta aurantia
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Spirochetes;
Spirochaetales; Spirochaetaceae.
REFERENCE 1 (sites)
AUTHORS Brahamsha,B. and Greenberg,E.P.
TITLE Cloning and sequence analysis of flaA, a gene encoding a
Spirochaeta aurantia flagellar filament surface antigen
JOURNAL J. Bacteriol. 171, 1692-1697 (1989)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1425)
AUTHORS Greenberg,E.P.
TITLE ;
JOURNAL Unpublished (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol. 171,
1692-1697 (1989)] kindly provided
by E.P.Greenberg, 26-APR-1989.
FEATURES Location/Qualifiers
CDS 209..1222
/note="flagellar filament surface antigen precursor"
/codon_start=209
sig_peptide 209..271
/note="put. flagellar filament surface antigen signal
peptide"
/codon_start=209
mat_peptide 272..1219
/note="put. flagellar filament surface antigen"
/codon_start=272
BASE COUNT 304 a 466 c 375 g 280 t
ORIGIN 73 bp upstream of NruI site.
1 aaaagttcct tgattcccga ccccgatgtt ctatattctc ccgtaaaggc cctcgagatt
61 ctggagacca ccgtgatggc gaactttccg ggaatctcga aggtgagggt gtcggtggcg
121 gagagccgcc ggggatttgt cgttgacaaa ggatcaagca tccatataat gaatttcagc
181 agaatccaag gtaaataagg agcactggat gaaaaggttc ttcgcgattc tgggggctgc
241 cctgttcgtg ggtaacagtg gtgccttcgc agaacaagct actctcattg atttctctaa
301 gttggttggc gaaggtaaca ccgggttgca cgctccgacg acgatcgact acagccgcca
361 ggctgggtcg gcctacagtg ccgaagataa ggcagcgatg aagatttctc tggccattcc
421 cagctgggag atcgagttgg cttcttcgtc ccagaccgtc gagaaccaga ctctttcgtt
481 ggtgaccgcc gctcccgtga agcaggacgc cgcccgctac ggtggtgaaa ccgtcatggg
541 tgttcggatt cacttcccct cgttcggcat caactccttt gccgtcatca agcctccgtt
601 caccatcccc gcctacgcca ccctcggcga cgccacggcc cagaacgcgg tggccggtgg
661 tcagttcgac ggcttcggcg tcctcaagaa cgtcggcgtc atcaagtcca tccagatcaa
721 cattctcgga cgcaactacc tgaaccgcct gtccctcctc ctcgaggacc agaacggcga
781 tgagcgcgag atcgtcatgg gctacctgaa cttcgacggt tggaagtcgc tccagtggaa
841 caaccccaac taccagaccg aagtccgcaa ccgcgacctt cagatcgtgc ccctctaccc
901 ccggtcggct cccctcatca agctcaaggg catcaagatc caccgcgacg gttcccagga
961 aggcggcgac atcgtctcct acatcaagga catcaaggtg atctacgacc aggccgtggt
1021 tgaccgcaac agcgacgtcg acgacgaggc catctggggc atcctccgcc agcgcgaaga
1081 gcagtaccgc aacttcgaac tcgccaagct cggtaacctc caggttctcc gcagcctcga
1141 gaagaagaag atggccaagg aagctgattt cgaccaggcc gctcccgccg ctgccgctgc
1201 tcgggccccc gctacgaact aatctcggat ctccgaagaa gccggctctc ttgggagccg
1261 gctttttttt tcaccacgac ttgaggaaag cccccatgaa cagccccgca aaccccagcc
1321 tcgcctccac cgttgcccag accctaggcc tgcccggtcc tgcggtgacc acggtcttcg
1381 agcttctgga ccaagaccac accatcccct tcatcgcccg gtacc
//