GenBank-Updates@genbank.bio.net (02/01/91)
LOCUS STRLYTPN 2376 bp ds-DNA BCT 01-FEB-1991
DEFINITION S.pneumoniae autolysin (lyt) gene, complete cds.
ACCESSION M13812 M55414 M55415
KEYWORDS amidase; autolysin.
SOURCE S.pneumoniae (strain Rst7) DNA, clone pGL80.
ORGANISM Streptococcus pneumoniae
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Streptococcaceae.
REFERENCE 1 (bases 1 to 2376)
AUTHORS Garcia,P., Garcia,J.L., Garcia,E. and Lopez,R.
TITLE Nucleotide sequence and expression of the pneumococcal autolysin
gene from its own promoter in Escherichia coli
JOURNAL Gene 43, 265-272 (1986)
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 2376)
AUTHORS Diaz,E. and Garcia,J.L.
TITLE Characterization of the transcription unit encoding the major
pneumococcal autolysin
JOURNAL Gene 90, 157-162 (1990)
STANDARD full staff_entry
COMMENT Draft entry and sequence in computer-readable form [Gene 43,
265-272 (1986)] kindly
provided by R.Lopez, 01-DEC-1986.
A potential ribosome binding site is located at positions 142-146.
FEATURES Location/Qualifiers
-35_signal 305..310
/gene="lytA"
-35_signal 314..319
/gene="lytA"
-10_signal 328..334
/gene="lytA"
-10_signal 335..340
/gene="lytA"
CDS 343..456
/note="ORF"
/codon_start=343
-35_signal 349..354
/gene="lytA"
-10_signal 372..377
/gene="lytA"
CDS 584..1540
/gene="lytA"
/codon_start=584
/product="autolysin"
BASE COUNT 773 a 378 c 521 g 704 t
ORIGIN 1 bp upstream of HindIII site.
1 gatccttcct ctagtttcta gctttattga actaatcgga aaaatcgttt ttgtggtttt
61 gattattcct tgggcaggat ataagggtgt tatcctttgt gaacctctta tctgggttgc
121 catgacagtt caactgtact tctcattatt ccgtcatccc ttgataaaag aaggcaaggc
181 aatcttggca accaaagtgc aatcctagtt ggatttactg aataaaatcc atttcctcta
241 gtgaaaatcg aaaaaacttg tgttctcgtc tttagtttgg tgttgaaaat agtttaacag
301 acttttgact tcttttatat gatataataa agtatagtat ttatgaaaag gacatataga
361 gactgtaaaa atatactttt gaaaagcttt ttagtctggg gtgttattgt agatagaatg
421 cagaccttgt cagtcctatt tacagtgtca aaatagtgcg ttttgaagtt ctatctacaa
481 gcctaatcgt gactaagatt gtcttctttg taaggtagaa ataaaggagt ttctggttct
541 ggattgtaaa aaatgagttg ttttaattga taaggagtag aatatggaaa ttaatgtgag
601 taaattaaga acagatttgc ctcaagtcgg cgtgcaacca tataggcaag tacacgcaca
661 ctcaactggg aatccgcatt caaccgtaca gaatgaagcg gattatcact ggcggaaaga
721 cccagaatta ggttttttct cgcacattgt tgggaacggt tgcatcatgc aggtaggacc
781 tgttgataat ggtgcctggg acgttggggg cggttggaat gctgagacct atgcagcggt
841 tgaactgatt gaaagccatt caaccaaaga agagttcatg acggactacc gcctttatat
901 cgaactctta cgcaatctag cagatgaagc aggtttgccg aaaacgcttg atacagggag
961 tttagctgga attaaaacgc acgagtattg cacgaataac caaccaaaca accactcaga
1021 ccacgttgac ccttatccat atcttgctaa atggggcatt agccgtgagc agtttaagca
1081 tgatattgag aacggcttga cgattgaaac aggctggcag aagaatgaca ctggctactg
1141 gtacgtacat tcagacggct cttatccaaa agacaagttt gagaaaatca atggcacttg
1201 gtactacttt gacagttcag gctatatgct tgcagaccgc tggaggaagc acacagacgg
1261 caactggtac tggttcgaca actcaggcga aatggctaca ggctggaaga aaatcgctga
1321 taagtggtac tatttcaacg aagaaggtgc catgaagaca ggctgggtca agtacaagga
1381 cacttggtac tacttagacg ctaaagaagg cgccatggta tcaaatgcct ttatccagtc
1441 agcggacgga acaggctggt actacctcaa accagacgga acactggcag acaggccaga
1501 attcacagta gagccagatg gcttgattac agtaaaataa taatggaatg tctttcaaat
1561 cagaacagcg catattatta ggtcttgaaa aagcttaata gtatgcgttt tcttgtggag
1621 atatttcctt caatgtttgc tactatatta aacaaaaatc agaaagcaaa ctagaaagtt
1681 atgctcaaat aaaatctaaa tttgacaatg taaaccgagt cggatagctt taagtactgt
1741 tttgaggttg aagatacgat ttttgatagg aactcatcaa ttttagattt ttaagcagca
1801 tcaataaatt gcttccttgt tttgtcataa tttttttatt taaaaaatta tgacaaaagt
1861 gtgctattct ttttatgaga ggtgtatgaa tatgataaat gtatgtgata aatgtatgtg
1921 atgttggaaa aagaataaaa gaacttagaa tatcttcaaa tcttactcaa gataagattg
1981 ctgaatattt gtctttatat ttgtctttga atcaaagcat gattgccaaa atggaaaaag
2041 gtgaaaggaa tatcacggat ggatttaagt aataaagctt caaatcttag aaaaaagttg
2101 ggagctgatg gtgaagcgcc gatagatatt tttaaattgg tacaaaagat agaaaatttg
2161 acgctggtat tttatggact cggaaagata ttagcggagt ctgttataaa ggaactcagt
2221 tcagtctcat tgcagtcaat tcagacatgt cattaggaag gtaaagattt tctttagcac
2281 atgaactgta tcatctttat tatgatgagg tgaagaagag ttcagtcagt cttatcttga
2341 ttggtgaagg agatgaaact gaaagaaaag cggatc
//