GenBank-Updates@genbank.bio.net (02/05/91)
LOCUS BMEGDH1 2834 bp ds-DNA BCT 05-FEB-1991
DEFINITION B.megaterium glucose dehydrogenase gene and ORFs.
ACCESSION D90043
KEYWORDS glucose dehydrogenase.
SOURCE Bacillus megaterium (strain IAM1030) DNA.
ORGANISM Bacillus megaterium
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2834)
AUTHORS Mitamura,T., Ebora,R.V., Nakai,T., Makino,Y., Negoro,S., Urabe,I.
and Okada,H.
TITLE Structure of isozyme genes of glucose dehydrogenase Bacillus
megaterium IAM1030
JOURNAL J. Ferment. Bioeng. 70, 363-369 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Toshihide Mitamura
Department of Fermentation Technology
Osaka University
2-1 Yamada-oka
Suita, Osaka 565
Japan
Phone: 06-877-5111 x4373
Fax: 06-876-90
FEATURES Location/Qualifiers
misc_signal 89..94
/note="put. -35 region for ORF1"
misc_signal 116..121
/note="put. -10 region for ORF1"
misc_signal 167..172
/note="ORF2 ribosome binding site"
CDS 181..867
/note="ORF1"
misc_signal 893..928
/note="termination signal"
misc_signal 972..977
/note="put. -35 region for ORF2"
misc_signal 997..1003
/note="put. -10 region for ORF2"
misc_signal 1070..1076
/note="ORF2 ribosome binding site"
CDS 1086..1946
/note="ORF2"
misc_signal 1949..1954
/note="glucose dehydrogenase ribosome binding site"
CDS 1964..2749
/note="glucose dehydrogenase (EC 1.1.1.47)"
misc_signal 2751..2783
/note="termination signal for glucose dehydrogenase"
BASE COUNT 896 a 442 c 644 g 852 t
ORIGIN
1 gatcaggtag cgagaatctt tgatgaaggt ttttcaacca aagcaaagga aaatagagga
61 attggtttgc atttagtaaa acaaattgtt gaaaaaggaa acggtcagat cgaagtagag
121 tcagaattag atgttggaac gacttttatc attacattct ttttataggg ggagtgggaa
181 atgaataaaa aagcatggac cgtgcttctc atagaagacg atcctatggt acaagaagtg
241 aaccgccaat ttattgaaca agttgaaggg ttcactgtta tcgctgcagc ttcgaatggt
301 ttagaggggg tacagctcat taaacagcat cagcctgatt taacgattat tgatatgtat
361 atgcctagtc aagatggctt aaccacctta cagcaaattc gagcaaatgg ctataaaaca
421 gacgtgatag cagttacggc tgcaagtgat attgaaaccg tacgcaaagt tcttcaatat
481 ggcgctgtgg attatattat gaaaccgttc aagtttgaac gaatgaagca agcgcttgag
541 cagtatcgtt cgtttcaagt taaaataagt caaaaagaac atattactca gtctgaatta
601 gattctatgc tgtttcagca attcgaagaa aaagccgatt tgcttcccaa ggggctaaat
661 gcggttacgt taaggaggat acaacaatat ctttccgaac aaaatcatcc aatttctgct
721 gaagaagtgg cggacggcgt aggaattgcg cgtgttacag caagaaggta tttagagttt
781 ttagaacagg aaaacgagct gaaattatca gttgaatacg gcagagtggg gagacctatt
841 aatcgctata tgttaaaaat aaattaaatc atacagaaca gcttttattt ggaaaagctg
901 tttttttgcg ttagaaagta tatctttttc tctcctagaa caaattaagg tatacagttt
961 tcgctaccca aagaatattt cgtgcggtca ttaatccata aaatgtccct gaaaaggatt
1021 aatggcggaa aaattgggga atatgcactt tgacatttaa ttttaacaca ggaaggtttt
1081 gaaacatgga catattttta gccgtcttac cagccatatt ttggggaagc attgtgcttt
1141 ttaatgtgaa actaggcgga ggaccttata gtcaaacgct tggaaccaca ttgggagctt
1201 taattttctc catcggtatt tatatttttg tacaccctac gtttacacct ttaatctttg
1261 gggttggagt tgtttcgggg ctattttggg cagttggaca aagtaatcag ctgaaaagta
1321 ttgatttaat tggagtttct aaaacgatgc ctatttcaac ggggcttcag ttagtttcca
1381 cttcattatt tggagtaatt gtgtttcacg agtggtctac aaaaacttca atcattcttg
1441 gtgtgctcgc tcttatcttt attattgtag ggattgtttt agcatcactt caaagcaaag
1501 aagagaaaga ggctgaagaa ggaaaaggaa acttcaaaaa aggaattgtt attttattaa
1561 tttcaaccgt tggttattta gtttatgttg tagtagcccg tctatttaat gtagacggat
1621 ggtcggcttt attacctcaa gcaattggta tggttattgg aggagtattg ctgacgttca
1681 agcataagcc atttaataaa tatgcaattc gcaacattat cccaggtctt atttgggccg
1741 ctggtaatat gtttttattc atctcacaac ctaaagtagg cgtagcgaca agcttttcgc
1801 tttctcaaat gggaatcgtc atttcaacat taggcgggat cattatttta ggtgagaaga
1861 aaacgaagcg tcagttagtt gggattatta ttgggattat actgatcatc atagcaggag
1921 tcatgttagg gctcgccaaa agctaactag gaggttatta acaatgtata aagatttaga
1981 agggaaagta gttgtcataa caggttcatc taccggttta ggaaaagcaa tggcgattcg
2041 ttttgcgaca gaaaaagcta aagtagttgt gaattatcgt tctaaagaag aagaagctaa
2101 cagcgtttta gaagaaatta aaaaagtcgg cggagaggca attgccgtta aaggtgacgt
2161 aacagttgag tctgacgtga tcaatttagt tcaatcttct attaaagaat ttggaaagtt
2221 agacgttatg attaataacg caggaatgga aaatccggtt tcatctcatg aaatgtcttt
2281 aagcgattgg aataaagtaa ttgatacgaa cttaacggga gcatttttag gcagccgtga
2341 agcgattaaa tattttgtgg aaaatgatat taagggaaca gttattaaca tgtcgagtgt
2401 tcacgagaaa attccttggc cattatttgt tcattacgca gcaagtaaag gcggaatgaa
2461 gctcatgacc gaaacacttg cattagaata cgctccaaaa ggtattcgtg taaataacat
2521 tggaccggga gcgattaata caccgattaa cgctgagaaa tttgctgatc ctgagcagcg
2581 tgcggatgta gaaagcatga ttccaatggg atacattgga gagccggaag aaattgcagc
2641 ggttgctgca tggctagctt cttcagaggc aagttatgta acagggatta cgctctttgc
2701 tgacggcggt atgacccagt acccatcatt ccaagcagga cgcggataag aaaaaacgca
2761 ctctataata gagtgcgttt tttagtttcc ctgagctttt ttttggttct taggagctga
2821 ctggtgttga attc
//