GenBank-Updates@genbank.bio.net (02/06/91)
LOCUS BSUAPKII 4907 bp ds-DNA BCT 06-FEB-1991
DEFINITION B.subtilis thioredoxin (trx), uvrB and aspartokinase II genes,
complete cds.
ACCESSION J03294 M26384
KEYWORDS aspartokinase; aspartokinase II; attenuator; kinase; promoter;
thioredoxin; uvrB protein; uvrC protein.
SOURCE B.subtilis (strain 168) DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (sites)
AUTHORS Chen,N.Y., Hu,F.M. and Paulus,H.
TITLE Nucleotide sequence of the overlapping genes for the subunits of
Bacillus subtilis aspartokinase II and their control regions
JOURNAL J. Biol. Chem. 262, 8787-8798 (1987)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Chen,N.-Y., Zhang,J.-J. and Paulus,H.
TITLE Chromosomal location of the Bacillus subtilis aspartokinase II gene
and nucleotide sequence of the adjacent genes homologous to uvrC
and trx of Escherichia coli
JOURNAL J. Gen. Microbiol. 135, 2931-2940 (1989)
STANDARD full staff_review
REFERENCE 3 (sites)
AUTHORS Zhang,J.-J., Hu,F.-M., Chen,N.-Y. and Paulus,H.
TITLE Comparison of the three aspartokinase isozymes in Bacillus subtilis
Marburg and 168
JOURNAL J. Bacteriol. 172, 701-708 (1990)
STANDARD full staff_review
REFERENCE 4 (sites)
AUTHORS Lu,Y., Chen,N.-Y. and Paulus,H.
TITLE Identification of aecA mutations in Bacillus subtilis as nucleotide
substitutions in the untranslated leader region of the
aspartokinase II operon
JOURNAL J Gen Microbiology (1991) In press
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence of [J. Biol. Chem. 262,
8787-8798 (1987)] kindly provided
by H.Paulus, 15-APR-1987. Draft entry and computer-readable
sequence [J. Gen. Microbiol. (1989) In press] kindly submitted by
H.Paulus, 20-JUL-1989.
The B.subtilis uvrB gene is homologous to the E.coli uvrC gene.
FEATURES Location/Qualifiers
CDS 11..325
/note="thioredoxin (put.)"
CDS 461..2257
/note="uvrB protein (EC 4.1.99.3)"
allele 2189..2190
/note="cc in wt; ccc in strains Marburg and GSY225"
allele 2199..2201
/note="ggc in wt; gc in strains Marburg and GSY225"
misc_signal 2235..2263
/note="ask promoter"
mRNA 2272..>3828
/note="ask mRNA"
allele 2305..2305
/note="t in wt; a in strain KA120 (allele aecA5)"
allele 2310..2310
/note="g in wt; a in strain VB217 (allele aecA3)"
allele 2310..2310
/note="g in wt; a in strain KA120 (allele aecA5)"
CDS 2352..2426
/note="aspartokinase II (ask) attenuator"
misc_signal 2352..2539
/note="ask attenuator"
allele 2467..2467
/note="c in wt; t in strain FB59 (allele aecA4)"
CDS 2602..3828
/note="aspartokinase II alpha subunit (EC 2.7.2.4)"
CDS 3337..3828
/note="aspartokinase II beta subunit"
BASE COUNT 1532 a 998 c 1117 g 1260 t
ORIGIN 1 bp upstream of EcoRI site; 253 degrees on Piggot-Hoch map.
1 gaattcaata atggctatcg taaaagcaac tgatcaatct ttctcagctg aaacaagcga
61 aggcgtcgta ctggcagact tctgggctcc ttggtgcgga ccttgtaaaa tgattgcacc
121 tgttcttgaa gaattggatc aagaaatggg agacaaactg aaaatcgtaa aaatcgatgt
181 agacgaaaac caagaaactg ccggaaaata cggcgttatg agcatcccga ctcttcttgt
241 gttaaaagac ggagaagtag ttgaaacttc agtcggcttc aaaccaaaag aagcgcttca
301 agagcttgta aacaaacatc tttaattttc cgctgcttac atgccagagc gattccgatt
361 gagggatcgc tttttttatt cgccaattgt gaaccataga gaaaaacaaa cgttcgtgtt
421 aaactggaaa taaaggatta aacataagga agggtaacat atgaacaaac aactgaaaga
481 aaaactcgcc ctccttcctg atcaaccggg ttgttatctc atgaaggacc ggcagcagac
541 tgtgatctac gtagggaaag caaaagtgct gaaaaacaga gtgcgctcct acttcaccgg
601 ttctcacgac gcgaaaaccc aaaggcttgt gacggaaatc gaggattttg aatatattgt
661 gacgtcctcc aatcttgaag cgcttatttt agaaatgaat ctgatcaaaa agcatgatcc
721 gaaatacaac gtcatgctca aagacgacaa aacctatcct ttcataaaac tcacccatga
781 acgccaccca aggctgattg tcacccgcaa tgtcaaaaaa gacaaagggc gctatttcgg
841 gccgtatccg aatgtacagg cagcaaggga aacaaaaaaa ctgctcgacc gtctgtatcc
901 tctcagaaaa tgctccaagc tcccggacag agtgtgcctt tactatcatc tgggccaatg
961 ccttgctccg tgtgtaaagg atatttccga agagacgaac agagagctgg ttgaaagcat
1021 tacacgtttc ttaaggggcg gctataatga ggtcaaaaaa gagcttgaag agaaaatgca
1081 tgaagctgct gagaatcttg agtttgaacg agcaaaagag cttcgtgacc aaattgccca
1141 tattgaatca acgatggaaa aacaaaaaat gacgatgaac gatctagtcg accgtgatgt
1201 gttcgcgtac gcgtacgata agggctggat gtgcgtgcag gtctttttca tccgccaagg
1261 aaagctcatt gaacgcgatg tcagcatgtt cccgctttat caggaagcag atgaagagtt
1321 cctcacgttc atcgggcagt tctattcaaa aaacaaccac ttccttccga aggagatttt
1381 agttccggac agcatagatc aatctatgat cgagcagcta ctggaaacaa acgttcacca
1441 gccgaaaaaa ggcccgaaaa aagaactgct catgcttgcc cataaaaatg cgaaaatcgc
1501 attgaaagaa aaattctctt taatcgaacg ggacgaggag cgttcaatcg gggcagtgca
1561 gaaactaggt gaggcattaa atatttatac gccgcacaga attgttgcgt ttgataactc
1621 aaatatacag gggacaaacc cggtttccgc gatgattgtg tttatcgatg gcaaaccata
1681 caaaaaggaa taccgcaaat ataaaatcaa aactgttaca gggccggatg attacggttc
1741 tatgagagag gttgtcagaa gacgctatac gagagtgctt cgtgagaatc tgccgctgcc
1801 cgatctgatt atcattgacg gaggaaaagg gcagatcaac gcagcaaggg atgtcattga
1861 aaatgaactc ggcttggata tcccgatcgc cggtttagcg aaagatgaaa aacacagaac
1921 ctcaaattta ctgatcggtg atccgctgga ggtggcgtat ctggaacgaa acagccagga
1981 attttacctc ctgcagcgca ttcaggacga ggtgcaccgt tttgcaatca gttttcacag
2041 gcaaatccgg ggaaaaagcg cgtttcaatc cgttttggac gacatcccgg gtatcggaga
2101 gaaaagaaag aaaatgctgt taaagcattt cggttccgtt aaaaaaatga aggaagcaag
2161 ccttgaggac atcaaaaaag ccggtgttcc gcagcggcgg ctcagctcct ttacgacaaa
2221 ttgcaaaaat aatgttgtcc ttttaaataa gatctgataa aatgtgaact aatttcatag
2281 ttagatcgtg ttatatggtg aagatagagg tgcgaacttc aagagtatgc ctttggagaa
2341 agatggattc tgtgaaaaag gctgaaaggg gagcgtcgcc gaagcaaata aaaccccatc
2401 ggtattattt gctggccgtg cattgaataa atgtaaggct gtcaagaaat cattttcttg
2461 gagggctatc tcgttgttca taatcattta tgatgattaa ttgataagca atgagagtat
2521 tcctctcatt gcttttttta ttgtggacaa agcgctcttt ctcctcaccc gcacgaacca
2581 aaatgtaaag ggtggtaata catgggtctt attgtacaaa aattcggagg cacttccgtc
2641 ggctcagtcg aaaaaattca aaatgcggca aaccgcgcaa ttgcagaaaa acagaaaggc
2701 catcaagtcg ttgtcgtcgt ttcagcaatg ggaaaatcca ctgacgaatt ggtcagcctt
2761 gcaaaagcta tttctgacca gccgagcaaa cgcgaaatgg atatgctgct ggcgacaggc
2821 gagcaggtca cgatttcact cctttcaatg gcattgcagg aaaaaggcta tgacgctgtg
2881 tcttatactg gctggcaggc gggaatccgt acggaggcca ttcacggaaa cgccagaatt
2941 acagatatcg acacttcggt tttagcagac cagcttgaaa aaggaaaaat tgtcattgtt
3001 gcaggattcc aaggcatgac agaggattgt gaaattacga cattgggccg gggcggttca
3061 gatacaacag cagttgcttt ggctgccgct ttaaaagttg ataaatgtga catatacaca
3121 gacgtgccag gcgtgtttac aaccgatccg cgctatgtga aatcagcgag aaaacttgag
3181 ggcatctcat acgatgaaat gcttgagctt gccaatttag gcgccggtgt tcttcatccg
3241 agagcagttg agttcgcgaa aaattaccaa gtgccgttag aagtgcgttc aagtacagaa
3301 accgaagcgg gaacattaat tgaggaggaa tcatccatgg aacagaattt aattgtcaga
3361 ggcattgcat ttgaagatca aatcacaaga gtaaccattt acgggctgac tagcggcctg
3421 acaactttgt ctactatttt tacaacactt gccaaaagaa acataaacgt ggatatcatt
3481 atccaaacgc aggccgagga caagactgga atttccttct ctgtcaaaac agaagatgca
3541 gaccaaaccg ttgcggtgct tgaagagtat aaagacgcgc tggaatttga gaaaatcgag
3601 acagaaagca aattggctaa agtatctatt gttggatccg gcatggtctc aaatcccggt
3661 gtagcggctg aaatgtttgc tgtactggcg caaaaaaaca ttttaatcaa aatggtcagc
3721 acatctgaaa tcaaagtgtc aacagtcgta agcgaaaatg acatggtgaa agcagtcgag
3781 tcgcttcacg atgcatttga gctttcaaaa cacccttcag ctgtgtaatg acaatcaaaa
3841 aggcgggact atgcagtcac cgcctttttg attacactgg gtctttcata tcccatttca
3901 cagtcagaac cactttatcg gctctctttt tcacttgttc atatgattcg gcaatctgat
3961 cattcatcaa ttgaatttgt tcagcgataa aaccagcctc cagctgaaag caaggttctt
4021 tctgatgctt aagccggttg gaaatgatcg gcccttcaag ttcaaattca agctcctgtt
4081 ttttagcgct tacgttggtg agggttcccc agcccgcttc ttcaaagaag gccggaaatt
4141 cctcccatga ttcaagagga aacttacggg cgagatgttt tcctgcccaa tacatcatgg
4201 aagagtaatc ttggccaagc atatctggga gaacgatctc tcgtattagt tcgtaggcat
4261 aagcgttcat ttcaatttct tttaggttgt caatagatgc ttcaaattta gacttcatat
4321 ctgtctcccc tctctcctgc gtatattata gctcaaacag gggggaggat tacagaatga
4381 tcctgtaatt cttatgaaaa attaagcaag aaatatatat tgataaaata aaatttttca
4441 atcaactaat caattcggaa aattataatt tatgtacgcg ttttcttgac gcccttttga
4501 gggaggagta aaatgaaatt gtcaataaat cttaataaag tgcttacaat tgaaagaagt
4561 gggggaagag atttagcaca tttcgcactt atcaaacagg gggtaatgta aagtctggga
4621 acagagagtt ttattttcga agattgcatt ccttgcttgg cgtcataccg gtcggcatct
4681 ttcttattca gcatttagtc gtcaaccagt ttgccgcaag gggcgctgaa gcattcaata
4741 gcgctgctca ttttatggat agcctgcctt tcaggtatgc cttggaaatt tttattatct
4801 tcttaccatt aatttatcat gcagtttatg gtgtgtacat agcgtttact gcgaaaaata
4861 acgccggtca atacagctac atgagtttct ggctattcgt cctgcag
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