GenBank-Updates@genbank.bio.net (02/06/91)
LOCUS HUMP53MUT 72 bp ds-DNA PRI 06-FEB-1991
DEFINITION Human p53 protein intronic point mutation at the splice donor site
at the 3' end of exon 7. resulting in the retention of intron 7
sequence in H647 mRNA.
ACCESSION M55576
KEYWORDS p53 protein.
SOURCE Human lung cancer cell line H647, cDNA to mRNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 72)
AUTHORS Takahashi,T., D'Amico,D., Chiba,I., Buchhagen,D.L. and Minna,J.D.
TITLE Identification of intronic point mutations as an alternative
mechanism for p53 inactivation in lung cancer
JOURNAL J. Clin. Invest. 86, 363-369 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
exon 1..5
/gene="p23"
/codon_start=1
/partial
mutation 6..6
/note="g in wt; t in cancer cell line H647 causing a
readthrough of the exon/intron boundary at exon 7/intron 7
and ending at a premature stop codon at position 69."
/gene="p23"
/partial
BASE COUNT 9 a 33 c 15 g 15 t
ORIGIN
1 tccaggtcag gagccacttg ccaccctgca cactggcctg ctgtgcccca ccctctgctt
61 gccctctgac cc
//