GenBank-Updates@genbank.bio.net (02/06/91)
LOCUS HUMP53MUT 72 bp ds-DNA PRI 06-FEB-1991 DEFINITION Human p53 protein intronic point mutation at the splice donor site at the 3' end of exon 7. resulting in the retention of intron 7 sequence in H647 mRNA. ACCESSION M55576 KEYWORDS p53 protein. SOURCE Human lung cancer cell line H647, cDNA to mRNA. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 1 to 72) AUTHORS Takahashi,T., D'Amico,D., Chiba,I., Buchhagen,D.L. and Minna,J.D. TITLE Identification of intronic point mutations as an alternative mechanism for p53 inactivation in lung cancer JOURNAL J. Clin. Invest. 86, 363-369 (1990) STANDARD full staff_entry FEATURES Location/Qualifiers exon 1..5 /gene="p23" /codon_start=1 /partial mutation 6..6 /note="g in wt; t in cancer cell line H647 causing a readthrough of the exon/intron boundary at exon 7/intron 7 and ending at a premature stop codon at position 69." /gene="p23" /partial BASE COUNT 9 a 33 c 15 g 15 t ORIGIN 1 tccaggtcag gagccacttg ccaccctgca cactggcctg ctgtgcccca ccctctgctt 61 gccctctgac cc //