GenBank-Updates@genbank.bio.net (02/07/91)
LOCUS PSELIPAA 2628 bp ds-DNA BCT 07-FEB-1991
DEFINITION P.cepacia lipase (lipA) gene, complete cds and open reading frame
limA.
ACCESSION M58494
KEYWORDS lipase.
SOURCE P.cepacia DNA.
ORGANISM Pseudomonas cepacia
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 2628)
AUTHORS Jorgensen,S.T., Skov,K.W. and Diderichsen,B.
TITLE Cloning, sequence, and expression of a Lipase gene from Pseudomonas
cepacia: Lipase production in Heterologous hosts requires two
pseudomonas genes
JOURNAL J. Bacteriol. 173, 559-567 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 364..1458
/gene="lipA gene"
/codon_start=364
/product="lipase"
CDS 1462..2496
/gene="limA"
/codon_start=1462
BASE COUNT 426 a 873 c 950 g 379 t
ORIGIN
1 gtcgacaacg tgctgaacaa ggcctatacc gagcacctga acctcgccgg caacgcgggc
61 ttcggctatc cggcgaactc gccggtgatg gagccgggcc ggaccgcctg ggtgcgcgtg
121 agcgcgaagc tgtaacgcgt cgtgcgtgtg cgttgcagca tgcgcacgca tgaacgcggc
181 cccgcctgaa cgggcggggc gcgtcaaccg attagagaac cgtgtctagt cagggcgcaa
241 acgttcgcga gtcgtgattc actcccgcat tcgacgcaca cggtgcgcgc gacgttcgcg
301 atgcagggtg tgtgtcgatc cggcttcatt cgcaccggca gcacaataat caggagaaca
361 tgcatggcca ggacgatgcg ttccagggtg gtggcagggg cagtggcatg cgcgatgagc
421 atcgcgccgt tcgcggggac gaccgcggtg atgacgctcg cgacgacgca cgcggcaatg
481 gcggccaccg cgcccgccgc tggctacgcg gcgacgcgtt acccgatcat cctcgtgcac
541 gggctctcgg gtaccgacaa gtacgccggc gtgctcgagt attggtacgg catccaggag
601 gacctgcaac agaacggtgc gaccgtctac gtcgcgaacc tgtcgggttt ccagagcgac
661 gacggcccga acgggcgcgg cgaacagttg ctcgcttacg tgaagacggt gctcgcggcg
721 acgggggcga ccaaggtcaa tctcgtcggt cacagccagg gcggcctctc gtcgcgctat
781 gttgctgccg tcgcgcccga tctcgttgcg tcggtgacga cgatcggccc agccgatcgc
841 ggctccgaat tcgccgactt cgtgcaggac gtgctcgcgt acgatccgac cgggctttcg
901 tcatcggtga tcgccgcgtt cgtcaatgtg ttcgggatcc tgacgagcag cagccacaac
961 accaaccagg acgcgctcgc cgcactgcag acgctgacca ccgcacgggc cgccacgtac
1021 aaccagaact atccgagcgc gggcctgggt gcgccgggca gttgccagac cggtgcgccg
1081 accgaaaccg tcggcggcaa cacgcacctg ctgtattcgt gggccggcac ggcgatccag
1141 ccgacgctct ccgtgttcgg cgtcacgggc gcgacggaca cgagcaccct tccgctcgtc
1201 gatccggcga acgtgctcga cctgtcgacg ctcgcgctgt tcggcaccgg cacggtgatg
1261 atcaaccgcg gctccgggca gaacgacggg ctcgtgtcga agtgcagtgc gctgtacggc
1321 aaggtgctga gcacgagcta caagtggaac cacctcgacg agatcaacca gctgctcggc
1381 gtgcgcggcg cgtatgcgga agatcccgtc gcggtgatcc gcacgcatgc gaaccggctg
1441 aagctggcgg gcgtgtaatc gatgacggca cgaggaggac gcgcgccgct ggcgcgccgc
1501 gccgtggtct atggtgccgt ggggctggcg gcgattgccg gcgtggcgat gtggagcggc
1561 gcgggccggc atggcgggac gggcgcatcc ggcgagccgc cggatgcgtc ggcggcacgc
1621 ggaccggctg ccgcaccgcc gcaggccgcc gtgccggcaa gcacgagcct gccgccgtcg
1681 ctcgccggct ccagcgcgcc ccgcttgccg ctcgatgccg gcggccatct cgcgaaggcg
1741 cgcgcggtgc gggatttctt cgactactgc ctgaccgcgc agagcgacct gagtgcggcc
1801 gggctcgatg cgttcgtcat gcgcgagatt gccgcacagc tcgacgggac cgttgcgcag
1861 gccgaggcgc tcgacgtgtg gcaccggtat cgcgcgtatc tcgacgcact cgcgaaattg
1921 cgcgatgccg gcgcggtcga caagtcggac ctgggtgcat tgcagctcgc gctcgaccag
1981 cgcgcgtcga tcgcgtaccg gtggctcggc gactggagcc agccgttctt cggtgcggag
2041 caatggcggc agcgctacga cctcgcgcgg ctgaagatcg cgcaggaccc cgcgctgacg
2101 gatgcgcaga aggccgaacg gctcgcggcg ctcgaacagc agatgccggc cgacgaacgc
2161 gccgcgcagc agcgcgtcga ccggcagcgc gcggcgatcg accagatcgc gcaattgcag
2221 aagagcgggg cgacgcccga tgcgatgcgc gcacaactga cgcagacgct cggccccgaa
2281 gcggccgcgc gcgtcgcgca gatgcagcag gacgacgcat cgtggcagag gcgctacgcg
2341 gactacgcgg cgcagcgtgc gcagatcgag tcggccggcc tgtcgccgca ggatcgcgac
2401 gcgcagatcg ccgcgctgcg gcagcgcgtg tttacgaagc ccggcgaagc cgtgcgcgcg
2461 gcatcgctcg atcgcggggc gggcagcgcg cggtaacgct cgcggcgcgg gcggcacggc
2521 gcatgacggt cgttatgccg cgcgcgtgag atcgcgaggc aggtgttgct cgatggtatt
2581 cgcgagcgtg tcgaacgccg gccggatgcc ttcgtgcgcg acgcatgc
//