GenBank-Updates@genbank.bio.net (02/08/91)
LOCUS ECOREPFIC 3319 bp ds-DNA BCT 08-FEB-1991
DEFINITION E.coli Ent plasmid P307 basic replicon REPFIC.
ACCESSION M16167
KEYWORDS RepA1 protein; RepFIC; copA gene; repA1 gene; replication protein.
SOURCE Plasmid P307 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3319)
AUTHORS Saadi,S., Maas,W.K., Hill,D.F. and Bergquist,P.L.
TITLE Nucleotide sequence analysis of RepFIC, a basic replicon present in
IncFI plasmids P307 and F, and its relation to the RepA replicon of
IncFII plasmids
JOURNAL J. Bacteriol. 169, 1836-1846 (1987)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Maas,R., Oppenheim,J., Saadi,S., Fuchs,T. and Maas,W.K.
TITLE Isolation and properties of the RepA1 protein of the IncFII
replicon RepFIC.
JOURNAL Mol. Microbiol. (1991) In press
STANDARD full staff_review
COMMENT The sequence that will appear in Molecular Microbiology is the
corrected sequence for the one which appeared in J. Bacteriol 169,
1836-1846 (1987). This sequence, which has accession number
M16167, has been corrected in the database.
FEATURES Location/Qualifiers
-35_signal 125..130
-10_signal 148..154
RBS 182..187
CDS 192..449
/note="repressor of second repA1 promoter"
/gene="copB"
-35_signal 462..467
/gene="repA1"
-10_signal 485..490
/gene="repA1"
stem_loop complement(574..665)
/note="countertranscript; inhibits translation of repA1
transcript; copy number control; expresses
incompatibility."
RBS 742..745
/gene="repA1"
CDS 750..1772
/note="initiator protein of the replicon RepFIC"
/gene="repA1"
BASE COUNT 881 a 833 c 877 g 727 t 1 others
ORIGIN 3 bp upstream of SmaI site.
1 cccggggggc cggctgtttc tggcatttcc agcttttcag tcatttcatt atcaaaatca
61 cattaaacgg tcgtaatcag acatgatttg tgcgccaaca cagatcattg tcacaattct
121 caagtcgctg atttcaaaaa actgtagtat cctctgcgaa acgatccctg tttaagtatt
181 gaggaggcga gatgtcgcag acagaaaatg cagtgacttc ctcatcaggt aacaagcgtg
241 cataccggaa aggtaaccct gttccggcca gagagagaca aagggcttct ctagctcgca
301 gaagcaacac tcataaggct tttcatgcgg ttatccaggc ccggttaaaa gacaggctga
361 gtgaactggc agatgaggaa ggtattaccc aggcgcagat gcttgaaaaa ctgattgaat
421 cagagctgaa acgtagagcg actttgtaaa tattcacatt cttgcttatc tcaggcgtga
481 gtgatagatt gctgatcgtt taaggaattt tgtggctggc cacgccataa ggtggcaggg
541 aactggttct gatgtggatt tacaggagcc agaaaagcaa aaaacccgat aatcttcatc
601 tagtttggcg acgaggagaa gattaccggg gtccacttaa accgtatagc caacaattca
661 gctatgcggg gagtatagtt atatgcccgg aaaagttcaa gacttctttc tgtgctcact
721 ccacctgcgc attgtaagtg caggatggtg tggctgaaag atacatctca caaagacact
781 ggagtcagct tcctcccgaa gagcaaatcc gtgtctggga agactatgaa gcgggaaggg
841 cgaccacttt cctggttgaa ccggaaagga agcgcacaaa gcgtcgtcgt ggtgagcact
901 ccactaaacc caaatgcgaa aatccgacct ggtatcgtcc tgcgcgctat aaggcgctga
961 gcgggcagct cgggcaccgc tataaccgtc tggtgaaaaa ggacccggtg accggcgaac
1021 agagcctgcg catgcacatg tcgcgacatc ctttttacgt gcagaaacgg acgttcgctg
1081 gccgtaaata tgctttccgt ccggaaaaac aacgcctcct cgatgctgtc tggccggttc
1141 tggtcagttt tagtgatgcc ggcacacaca cagtggggat gagtgtttcc cgcctggcta
1201 aagaaatcag cccgaaagac agcaaaggaa aggttatccc cgaactcgag gtgacggtct
1261 cccggctttc ccgtctgctg gcagagcagg tgcgttttgg tgtgctgggt atgtcggaag
1321 aaacaatgtg ggaccgtgaa acccgccagc gtctgccacg ctatgtctgg ataacaccgg
1381 cagggtggca gatgctgggc gtcgacatgg ttaaacttca cgaacagcag cagaagcggc
1441 tgcgtgaaag tgaaatccgc cagcagctca tccgggaagg cgtactgcgt gaggatgaag
1501 atatctccgt acatgcggcc agaaaacgct ggtatctgca gcgcagccag gatgcgctga
1561 aacaccgtcg ggcgaaagct gcagcccgca agcgtgctaa tcttctgaag aagctacctg
1621 ccgaccagca gatttatgag atgtcacagc atatcctgaa gcgtatgccg ccggatgaag
1681 cctactggtg cacgccggaa cgcctgcagc aactggccat cagggagctg tatcagcttg
1741 aactgacgct ggctgcaccg ccaccgcact agacagcacc atttcctcag cactgaatca
1801 gtcgcatccg gggctttcgg cgctggttcc gttcgaccag aaactccccg taaccacctg
1861 aaatatcctc atctggccat atctgacgca aagtcactcc cctgtcgtca gaatgtggcc
1921 acgtcgtttc agttatccac ataaatccgc aaataaagag ttttaagaag ctgcaaacca
1981 aaaacagcaa acctgcaata tagtcttacc ccagttactt aatcccctgc gttgcttcgc
2041 ctcagggaaa gtctttatct ctgaaacgcc tatgaacaac gtacaaagag gccttcgctt
2101 gcaggcaaag gccgctcaga ctttaaaagt actccacgca aggcggcccc gacggagcca
2161 ttttagttag aacactcaaa tgcgaccacc aagaaaaacc tagtcccgtg cagaactgaa
2221 accacaaagc cccccccctc ataacttaaa agcgcccccg gcccgaaagg gccggaacag
2281 agtcgctttt aattatgaat gttgtaacta cattatcatc gctgtcagtc ttctggctgg
2341 acgtactgag tacacgctcg taagcggccc tgacggcccg ctaacgcgga gatacgcccc
2401 gactgcgggt aaacccttgt cgggaccact ccgaccgcgc acagaagctc tgtcatggct
2461 gaaagcgggt atagcttagc aggaccggga tgagtaaggt gaaatctatc aatacgtacc
2521 ggcttacgcc gggcttcggc ggttttactc cggtataata tgaaacaaca aagtgccgcc
2581 ttacatgccg ctggcgcggc atatcttggt gacaatatct gaatcgttat atactgcgta
2641 tatacgtagt aatgacgagg tgataaatgg cacaggttaa tatgagttta agaatcgacg
2701 ctgaactgaa ggatgctttt atggctgctg caaaaagcat ggaccgtaat ggctctcagt
2761 taatccggga ttttatgcgc cagaccgttg aacggcagca taatacctgg ttccgtgacc
2821 aggttgcggc aggacgtcag caactcgaga gtgtgcttcc ccatgacatg gtcgaatctt
2881 ctgctgctgc atggcgggat gaaatgagca ggaaggttgc cgataaatga atggagatat
2941 tctggaccat gctggccagc ccaggacaga agcgcattcg tgagtacatt gcgagagaac
3001 ctgatggccg ccatgaactg gagaacggat tgttattcag ccagcagcct ggccgggcag
3061 ccgtataaag gccgcaaggc ttgaaggtac ccgggactgg ttattcaccc gccattttgt
3121 tctgggttta tgaggtcgat agccagtggg gaaaagtgta tatcctgcgt gtattgcata
3181 ctgcacagaa gtggccatag aggggctgtc cggatataaa aatgtctgac ggagatgcaa
3241 gaaccatgaa taatgatgag ctggccacca gacgtgcgca ggcgattgcc gaagacagat
3301 gtttctcgaa agcccntct
//