GenBank-Updates@genbank.bio.net (02/09/91)
LOCUS RATCKB 1444 bp ss-mRNA ROD 09-FEB-1991
DEFINITION Rat brain creatine kinase mRNA, complete cds.
ACCESSION M14400
KEYWORDS creatine kinase.
SOURCE Rat (Fisher, adult male) brain, cDNA to mRNA, clone
lambda-gt11-ckb-1.
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 67 to 1444)
AUTHORS Benfield,P.A., Henderson,L. and Pearson,M.L.
TITLE Expression of a rat brain creatine kinase-beta-galactosidase fusion
protein in Escherichia coli and derivation of the complete amino
acid sequence of rat brain creatine kinase
JOURNAL Gene 39, 263-267 (1985)
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 1444)
AUTHORS Benfield,P.A.
TITLE ;
JOURNAL Unpublished (1987) E.I.duPont de Nemours & Co. Wilmington, DE 19898
STANDARD full staff_entry
COMMENT Draft entry and printed copy of sequence for [Gene 39, 263-267
(1985)] kindly provided by
P.A.Benfield, 25-MAR-1987.
FEATURES Location/Qualifiers
mRNA <1..1444
/note="ck mRNA"
CDS 96..1241
/note="brain creatine kinase"
/codon_start=1
BASE COUNT 292 a 449 c 421 g 282 t
ORIGIN 3 bp upstream of HaeIII site.
1 cggccgtcgt tcttctgcgt gcgcccggag ctgcaagcac aagcagtctg cgttcctgct
61 ccgtcggaat cccgcctgcc gccgccgccg ccgccatgcc cttctccaac agccacaaca
121 cgcagaagct gcgcttcccg gccgaggatg aattccctga tctgagcagc cacaacaacc
181 atatggccaa ggtgctgacc cccgagctgt acgcggagct ccgtgccaag tgcacgccga
241 gcggctttac gttggacgac gccatccaga ctggcgtaga caatccgggc cacccgtaca
301 tcatgacagt gggtgcagtg gcgggcgacg aggagagtta cgacgtattc aaggaccttt
361 tcgaccccat catcgaggac cggcacggcg gctaccagcc cagtgatgag cacaagactg
421 acctcaaccc agacaacctg cagggcggcg atgacctgga ccccaactac gtgctgagct
481 cgcgggtgcg cacaggccga agcatccgcg gcttctgcct cccccctcac tgcagccgtg
541 gggagcgccg cgccatcgag aagctggcag tagaagccct gtccagccta gatggcgacc
601 tgtctggcag gtactatgcg ctcaagagca tgaccgaggc ggacgagcag cagctcattg
661 acgaccactt cctcttcgac aagcctgtgt cgcctctgct gctggcctcc ggcatggccc
721 gcgactggcc ggatgctcgc ggcatttggc acaatgacaa taagacgttc ctggtgtgga
781 tcaacgagga ggaccacctc cgggttatct ccatgcagaa agggggcaac atgaaggaag
841 ttttcacgcg attctgcact ggcctcactc agattgaaac tctcttcaag tctaagaact
901 atgagttcat gtggaaccct cacctgggct acatcctcac atgcccatcc aacctgggca
961 ctgggcttcg ggcaggcgtg cacatcaagc tgccccacct gggaaagcac gagaagttct
1021 cggaggtgct caagcgactg cggcttcaga agcgaggcac aggtggtgtg gacaccgctg
1081 ctgtgggtgg agtttttgat gtctccaacg ctgaccgcct gggcttctcg gaggtggagc
1141 tggtgcagat ggtggtggac ggagtgaagc tactcattga gatggagcag cggcttgagc
1201 agggtcagcc cattgacgac ctcatgcctg cccagaagtg aagccgtggc cctagccacc
1261 accaggctgc cgcttcctaa cttattaccc gggcagtgcc cgccatgcat ccttgatgtt
1321 tgccgcctgc cgggtgagcc ccttagcctc gctgtagaga cttctgtcgc cctgggtaga
1381 gcctttattt tttctgatgg ctaagctgtt gcagacactg aaataaatta gggtttggcc
1441 tgcc
//