GenBank-Updates@genbank.bio.net (02/06/91)
LOCUS SYNTDNA 6082 bp ds-DNA SYN 06-FEB-1991 DEFINITION Artificial T-DNA conferring expression of a luciferase/kanamycin-resistance fusion to transgenic plants. ACCESSION M61152 M38354 KEYWORDS NPTII; firefly luciferase; kanamycin resistance; transfer DNA. SOURCE Synthetic DNA. ORGANISM Artificial gene Artificial sequences; Genes. REFERENCE 1 (sites) AUTHORS Ditta,G., Stanfield,S., Corbin,D. and Helinski,D. TITLE Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti JOURNAL Proc. Natl. Acad. Sci. U.S.A 77, 7347-7351 (1980) STANDARD full staff_review REFERENCE 2 (sites) AUTHORS Gardner,R.C., Howarth,A.J., Hahn,P., Brown-Luedi,M., Shepherd,R.J. and Messing,J. TITLE the complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by m13mp7 shotgun sequencing JOURNAL Nucleic Acids Res. 9, 2871-2888 (1981) STANDARD full staff_review REFERENCE 3 (sites) AUTHORS Beck,E., Ludwig,G., Auerswald,E.-., Reiss,B. and Schaller,H. TITLE nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon tn5 JOURNAL Gene 19, 327-336 (1982) STANDARD full staff_review REFERENCE 4 (sites) AUTHORS Bevan,M., Barnes,W.M. and Chilton,M.-. TITLE structure and transcription of the nopaline synthase gene region of t-dna JOURNAL Nucleic Acids Res. 11, 369-385 (1983) STANDARD full staff_review REFERENCE 5 (sites) AUTHORS Lee,J.S., Brown,W.E., Graham,J.S., Pearce,G., Fox,E.A., Dreher,T.W., Ahern,K.G., Pearson,G.D. and Ryan,C.A. TITLE Molecular characterization and phylogenetic studies of a wound-inducible proteinase inhibitor I gene in Lycopersicon species JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83, 7277-7281 (1986) STANDARD full staff_review REFERENCE 6 (sites) AUTHORS de Wet,J.R., Wood,K.V., DeLuca,M., Helinski,D.R. and Subramani,S. TITLE Firefly luciferase gene: Structure and expression in mammalian cells JOURNAL Mol. Cell. Biol. 7, 725-737 (1987) STANDARD full staff_review REFERENCE 7 (sites) AUTHORS Barnes,W.M. TITLE ; JOURNAL Unpublished (1991) STANDARD full staff_review REFERENCE 8 (bases 1 to 6082) AUTHORS Barnes,W.M. TITLE Variable patterns of expression of Luciferase in transgenic tobacco leaves JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 9183-9187 (1991) STANDARD simple staff_entry COMMENT Sequence source are the following: bases 5-505 corresponds to 2596-2096 pTi T37 as numbered in [Bevan, et al.]; bases 646-1632 to 6492-7478 of CaMV1841 as numbered in [Gardner, et al.]J02046; bases 1698-3338 firefly luciferase codons 4 through 549 (last) nucleotides 312-1952 of reference [De Wet, et al.]; bases 3346-4125 NPTII (kanamycin resistance) codons, bp 166-945 of reference [Beck, et al.] J01834; bases 4125 1743 3' end of tomato protease inhibitor I gene, bp 4125-4840 of reference [Lee, et al.] M13938; conflict 4335-4340 TAATAA here is TAA in [Lee, et al.]; bases 4855-5538 3' end of nopaline synthase gene, bp 687-2 of reference [Bevan, et al.]; bases 5544-5573 LacZ DNA, 1390-1419 of accession J01636; bases 5572-6082 existing restriction sites [Barnes, et al.] in 10.2 kb of vector pRK252 [Ditta, et al.], not sequenced or mapped. The sequence at the end of this entry, containing many restriction sites spaced by arbitrary spans of 10 unknown nucleotides, is a computer-readable representation of the restriction sites in vector pRK252 [Ditta, et al.] DNA. pRK252 [Ditta, et al.] has been tested and found to lack sites for the following restriction enzymes (partial list [Barnes, et al.]): SacI, ClaI, HpaI, XbaI, KpnI, PstI, ScaI, NsiI, HindIII, BamH1, AflII. The Luc/Kan gene is not expressed in E.coli, but is expressed in Agrobacteri FEATURES Location/Qualifiers CDS 1660..4125 /codon_start=1660 /product="luciferase/kanamycin resistance protein" BASE COUNT 1626 a 1301 c 1321 g 1496 t 338 others ORIGIN 1 gaattcgagg atttttcggc gctgcgctac gtccgcgacc gcgttgaggg atcaagccac 61 agcagcccac tcgaccttct nnccgaccca gacgagccaa gggatctttt tggaatgctg 121 ctccgtcgtc aggctttccg acgtttgggt ggttgaacag aagtcattat cgcacggaat 181 gccaagcact cccgagggga accctgtggt tggcntgcac atacaaatgg acgaacggat 241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg 301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca 361 atctgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga 421 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg 481 ggtttctgga gtttaatgag ctaagggtac cctaagcaca tacgtcagaa accattattg 541 cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct 601 ccactgacgt tccataaatt cccctcggta tccaattaga gtctcagatc tcctttgccc 661 cggagatcac catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag 721 aaggtgacga taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa 781 agaatgctga cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct 841 acccgagtaa taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca 901 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa 961 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga 1021 ttggagtctc taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga 1081 tcgaggatct aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac 1141 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact 1201 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa 1261 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa 1321 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta 1381 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca 1441 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct 1501 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat 1561 aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc 1621 tatctctctc tagatctctc tcaatccaaa taatctgcaa tggcaattac cggatcttgg 1681 aggttactta tggatcccgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta 1741 gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct 1801 ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc 1861 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga 1921 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt 1981 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt 2041 atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg 2101 aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat 2161 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat 2221 gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat 2281 tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc 2341 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt 2401 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata 2461 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt 2521 caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa 2581 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca 2641 cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga 2701 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat 2761 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat 2821 accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt 2881 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg 2941 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc 3001 ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata 3061 ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc 3121 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag 3181 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg 3241 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag 3301 atcctcataa aggccaagaa gggcggaaag tccaaattct cgaggggatt gcacgcaggt 3361 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 3421 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 3481 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg 3541 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 3601 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 3661 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 3721 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 3781 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 3841 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 3901 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 3961 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 4021 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 4081 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaagctt gctaaggaaa 4141 taattgagaa ggaaaatcca tccataacta atattccaat attattgagt ggttctccaa 4201 ttacattaga ttatctatgt gatcgagttc gtctttttga taacatcttg ggctttgttg 4261 tacaaatgcc tgtggtgact taattaatgg accttattat tgaagtaatt aagcagccac 4321 aatatgtcaa aaaataataa ttagggttca tgtatgttca ttataatgca tctcttcatg 4381 tactcttact atatataatg gaaaaaataa gtgtggctta attacatgta ctcctctcct 4441 taattaattc ctttttacat gtggtactac gttattttga ttattttaat ttatttgtct 4501 gattttgact ataacatctt tggacatata tttaattaat gtgcgagagc taagatttga 4561 attgtaattg acaaattagt cccccaaccc cactacaccc caaaaataaa aataaaacgt 4621 gacaatgtgt tataattttt tggaataaat taagaaggtt aagggatcaa gtaaacaaaa 4681 aatatgtgtg gaaagataaa tatgtattta tctttaatta gaagttttga atttgaacat 4741 aaatatatat aaaaaaaggt tatctattaa ttgatctact ctaaagtgga tattgaatat 4801 caaataaaaa actaaaaaaa agtaaagcat aacaagatct ctagaagctt ccgtcgtcga 4861 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct 4921 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta 4981 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta 5041 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc 5101 atctatgtta ctagatcgat caaacttcgg tactgtgtaa tgacgatgag caatcgagag 5161 gctgactaac aaaaggtatg cccaaaaaca acctctccaa actgtttcga attggaagtt 5221 tctgctcatg ccgacaggca taacttagat attcgcgggc tattcccact aattcgtcct 5281 gctggtttgc gccaagataa atcagtgcat ctccttacaa gttcctctgt cttgtgaaat 5341 gaactgctga ctgcccccca agaaagcctc ctcatctccc agttggcggc ggctgataca 5401 ccatcgaaaa cccacgtccg aacacttgat acatgtgcct gagaaatagg cctacgtcca 5461 agagcaagtc ctttctgtgc tcgtcggaaa ttcctctcct gtcagacggt cgtgcgcatg 5521 tcttgcgttg atgaagctag cttgctggcg taatagcgaa gaggcccgca ccggaattcn 5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttaaan nnnnnnnnnt gatcannnnn 5641 nnnnntgatc annnnnnnnn ncgatcgnnn nnnnnnncag ctgnnnnnnn nnncctnnnn 5701 naggnnnnnn nnnngtcgac nnnnnnnnnn cccgggnnnn nnncccgggn nnnnnnnnna 5761 ggcctnnnnn nnnnngaann nnttcnnnnn nnnnngcggc cgcnnnnnnn nnngcggccg 5821 cnnnnnnnnn ngcggccgcn nnnnnnnnng ccnnnnnggc nnnnnnnnnn gccnnnnngg 5881 cnnnnnnnnn ngccnnnnng gcnnnnnnnn nngccnnnnn ggcnnnnnnn nnngccnnnn 5941 nggcnnnnnn nnnnccgcgg nnnnnnnnnn ccgcggnnnn nnnnnnccgc ggnnnnnnnn 6001 nnaatattnn nnnnnnnnaa tattnnnnnn nnnnaatatt nnnnnnnnnn aatattnnnn 6061 nnnnnnaata ttnnnnnnnn nn //
GenBank-Updates@genbank.bio.net (02/13/91)
LOCUS PWB146 6082 bp ds-DNA SYN 13-FEB-1991 DEFINITION Artificial T-DNA conferring expression of a luciferase/kanamycin-resistance fusion to transgenic plants. ACCESSION M61152 M38354 KEYWORDS NPTII; firefly luciferase; kanamycin resistance; transfer DNA. SOURCE Synthetic DNA. ORGANISM Artificial gene Artificial sequences; Genes. REFERENCE 1 (sites) AUTHORS Ditta,G., Stanfield,S., Corbin,D. and Helinski,D. TITLE Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77, 7347-7351 (1980) STANDARD full staff_review REFERENCE 2 (sites) AUTHORS Gardner,R.C., Howarth,A.J., Hahn,P., Brown-Luedi,M., Shepherd,R.J. and Messing,J. TITLE The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by m13mp7 shotgun sequencing JOURNAL Nucleic Acids Res. 9, 2871-2888 (1981) STANDARD full staff_review REFERENCE 3 (sites) AUTHORS Beck,E., Ludwig,G., Auerswald,E.A., Reiss,B. and Schaller,H. TITLE Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5 JOURNAL Gene 19, 327-336 (1982) STANDARD full staff_review REFERENCE 4 (sites) AUTHORS Bevan,M., Barnes,W.M. and Chilton,M.D. TITLE Structure and transcription of the nopaline synthase gene region of T-DNA JOURNAL Nucleic Acids Res. 11, 369-385 (1983) STANDARD full staff_review REFERENCE 5 (sites) AUTHORS Lee,J.S., Brown,W.E., Graham,J.S., Pearce,G., Fox,E.A., Dreher,T.W., Ahern,K.G., Pearson,G.D. and Ryan,C.A. TITLE Molecular characterization and phylogenetic studies of a wound-inducible proteinase inhibitor I gene in Lycopersicon species JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83, 7277-7281 (1986) STANDARD full staff_review REFERENCE 6 (sites) AUTHORS de Wet,J.R., Wood,K.V., DeLuca,M., Helinski,D.R. and Subramani,S. TITLE Firefly luciferase gene: Structure and expression in mammalian cells JOURNAL Mol. Cell. Biol. 7, 725-737 (1987) STANDARD full staff_review REFERENCE 7 (sites) AUTHORS Barnes,W.M. TITLE ; JOURNAL Unpublished (1990) STANDARD full staff_review REFERENCE 8 (bases 1 to 6082) AUTHORS Barnes,W.M. TITLE Variable patterns of expression of Luciferase in transgenic tobacco leaves JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 9183-9187 (1990) STANDARD simple staff_entry COMMENT Sequence source are the following: bases 5-505 corresponds to 2596-2096 pTi T37 as numbered in [Bevan, et al.]; bases 646-1632 to 6492-7478 of CaMV1841 as numbered in [Gardner, et al.]J02046; bases 1698-3338 firefly luciferase codons 4 through 549 (last) nucleotides 312-1952 of reference [De Wet, et al.]; bases 3346-4125 NPTII (kanamycin resistance) codons, bp 166-945 of reference [Beck, et al.] J01834; bases 4125 1743 3' end of tomato protease inhibitor I gene, bp 4125-4840 of reference [Lee, et al.] M13938; conflict 4335-4340 TAATAA here is TAA in [Lee, et al.]; bases 4855-5538 3' end of nopaline synthase gene, bp 687-2 of reference [Bevan, et al.]; bases 5544-5573 LacZ DNA, 1390-1419 of accession J01636; bases 5572-6082 existing restriction sites [Barnes, et al.] in 10.2 kb of vector pRK252 [Ditta, et al.], not sequenced or mapped. The sequence at the end of this entry, containing many restriction sites spaced by arbitrary spans of 10 unknown nucleotides, is a computer-readable representation of the restriction sites in vector pRK252 [Ditta, et al.] DNA. pRK252 [Ditta, et al.] has been tested and found to lack sites for the following restriction enzymes (partial list [Barnes, et al.]): SacI, ClaI, HpaI, XbaI, KpnI, PstI, ScaI, NsiI, HindIII, BamH1, AflII. The Luc/Kan gene is not expressed in E.coli, but is expressed in Agrobacterium FEATURES Location/Qualifiers CDS 1660..4125 /codon_start=1 /product="luciferase/kanamycin resistance protein" BASE COUNT 1626 a 1301 c 1321 g 1496 t 338 others ORIGIN 1 gaattcgagg atttttcggc gctgcgctac gtccgcgacc gcgttgaggg atcaagccac 61 agcagcccac tcgaccttct nnccgaccca gacgagccaa gggatctttt tggaatgctg 121 ctccgtcgtc aggctttccg acgtttgggt ggttgaacag aagtcattat cgcacggaat 181 gccaagcact cccgagggga accctgtggt tggcntgcac atacaaatgg acgaacggat 241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg 301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca 361 atctgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga 421 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg 481 ggtttctgga gtttaatgag ctaagggtac cctaagcaca tacgtcagaa accattattg 541 cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct 601 ccactgacgt tccataaatt cccctcggta tccaattaga gtctcagatc tcctttgccc 661 cggagatcac catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag 721 aaggtgacga taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa 781 agaatgctga cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct 841 acccgagtaa taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca 901 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa 961 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga 1021 ttggagtctc taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga 1081 tcgaggatct aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac 1141 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact 1201 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa 1261 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa 1321 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta 1381 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca 1441 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct 1501 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat 1561 aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc 1621 tatctctctc tagatctctc tcaatccaaa taatctgcaa tggcaattac cggatcttgg 1681 aggttactta tggatcccgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta 1741 gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct 1801 ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc 1861 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga 1921 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt 1981 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt 2041 atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg 2101 aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat 2161 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat 2221 gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat 2281 tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc 2341 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt 2401 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata 2461 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt 2521 caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa 2581 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca 2641 cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga 2701 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat 2761 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat 2821 accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt 2881 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg 2941 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc 3001 ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata 3061 ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc 3121 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag 3181 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg 3241 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag 3301 atcctcataa aggccaagaa gggcggaaag tccaaattct cgaggggatt gcacgcaggt 3361 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 3421 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 3481 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg 3541 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 3601 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 3661 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 3721 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 3781 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 3841 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 3901 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 3961 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 4021 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 4081 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaagctt gctaaggaaa 4141 taattgagaa ggaaaatcca tccataacta atattccaat attattgagt ggttctccaa 4201 ttacattaga ttatctatgt gatcgagttc gtctttttga taacatcttg ggctttgttg 4261 tacaaatgcc tgtggtgact taattaatgg accttattat tgaagtaatt aagcagccac 4321 aatatgtcaa aaaataataa ttagggttca tgtatgttca ttataatgca tctcttcatg 4381 tactcttact atatataatg gaaaaaataa gtgtggctta attacatgta ctcctctcct 4441 taattaattc ctttttacat gtggtactac gttattttga ttattttaat ttatttgtct 4501 gattttgact ataacatctt tggacatata tttaattaat gtgcgagagc taagatttga 4561 attgtaattg acaaattagt cccccaaccc cactacaccc caaaaataaa aataaaacgt 4621 gacaatgtgt tataattttt tggaataaat taagaaggtt aagggatcaa gtaaacaaaa 4681 aatatgtgtg gaaagataaa tatgtattta tctttaatta gaagttttga atttgaacat 4741 aaatatatat aaaaaaaggt tatctattaa ttgatctact ctaaagtgga tattgaatat 4801 caaataaaaa actaaaaaaa agtaaagcat aacaagatct ctagaagctt ccgtcgtcga 4861 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct 4921 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta 4981 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta 5041 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc 5101 atctatgtta ctagatcgat caaacttcgg tactgtgtaa tgacgatgag caatcgagag 5161 gctgactaac aaaaggtatg cccaaaaaca acctctccaa actgtttcga attggaagtt 5221 tctgctcatg ccgacaggca taacttagat attcgcgggc tattcccact aattcgtcct 5281 gctggtttgc gccaagataa atcagtgcat ctccttacaa gttcctctgt cttgtgaaat 5341 gaactgctga ctgcccccca agaaagcctc ctcatctccc agttggcggc ggctgataca 5401 ccatcgaaaa cccacgtccg aacacttgat acatgtgcct gagaaatagg cctacgtcca 5461 agagcaagtc ctttctgtgc tcgtcggaaa ttcctctcct gtcagacggt cgtgcgcatg 5521 tcttgcgttg atgaagctag cttgctggcg taatagcgaa gaggcccgca ccggaattcn 5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttaaan nnnnnnnnnt gatcannnnn 5641 nnnnntgatc annnnnnnnn ncgatcgnnn nnnnnnncag ctgnnnnnnn nnncctnnnn 5701 naggnnnnnn nnnngtcgac nnnnnnnnnn cccgggnnnn nnncccgggn nnnnnnnnna 5761 ggcctnnnnn nnnnngaann nnttcnnnnn nnnnngcggc cgcnnnnnnn nnngcggccg 5821 cnnnnnnnnn ngcggccgcn nnnnnnnnng ccnnnnnggc nnnnnnnnnn gccnnnnngg 5881 cnnnnnnnnn ngccnnnnng gcnnnnnnnn nngccnnnnn ggcnnnnnnn nnngccnnnn 5941 nggcnnnnnn nnnnccgcgg nnnnnnnnnn ccgcggnnnn nnnnnnccgc ggnnnnnnnn 6001 nnaatattnn nnnnnnnnaa tattnnnnnn nnnnaatatt nnnnnnnnnn aatattnnnn 6061 nnnnnnaata ttnnnnnnnn nn //