GenBank-Updates@genbank.bio.net (02/06/91)
LOCUS SYNTDNA 6082 bp ds-DNA SYN 06-FEB-1991
DEFINITION Artificial T-DNA conferring expression of a
luciferase/kanamycin-resistance fusion to transgenic plants.
ACCESSION M61152 M38354
KEYWORDS NPTII; firefly luciferase; kanamycin resistance; transfer DNA.
SOURCE Synthetic DNA.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (sites)
AUTHORS Ditta,G., Stanfield,S., Corbin,D. and Helinski,D.
TITLE Broad host range DNA cloning system for gram-negative bacteria:
Construction of a gene bank of Rhizobium meliloti
JOURNAL Proc. Natl. Acad. Sci. U.S.A 77, 7347-7351 (1980)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Gardner,R.C., Howarth,A.J., Hahn,P., Brown-Luedi,M., Shepherd,R.J.
and Messing,J.
TITLE the complete nucleotide sequence of an infectious clone of
cauliflower mosaic virus by m13mp7 shotgun sequencing
JOURNAL Nucleic Acids Res. 9, 2871-2888 (1981)
STANDARD full staff_review
REFERENCE 3 (sites)
AUTHORS Beck,E., Ludwig,G., Auerswald,E.-., Reiss,B. and Schaller,H.
TITLE nucleotide sequence and exact localization of the neomycin
phosphotransferase gene from transposon tn5
JOURNAL Gene 19, 327-336 (1982)
STANDARD full staff_review
REFERENCE 4 (sites)
AUTHORS Bevan,M., Barnes,W.M. and Chilton,M.-.
TITLE structure and transcription of the nopaline synthase gene region of
t-dna
JOURNAL Nucleic Acids Res. 11, 369-385 (1983)
STANDARD full staff_review
REFERENCE 5 (sites)
AUTHORS Lee,J.S., Brown,W.E., Graham,J.S., Pearce,G., Fox,E.A.,
Dreher,T.W., Ahern,K.G., Pearson,G.D. and Ryan,C.A.
TITLE Molecular characterization and phylogenetic studies of a
wound-inducible proteinase inhibitor I gene in Lycopersicon species
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83, 7277-7281 (1986)
STANDARD full staff_review
REFERENCE 6 (sites)
AUTHORS de Wet,J.R., Wood,K.V., DeLuca,M., Helinski,D.R. and Subramani,S.
TITLE Firefly luciferase gene: Structure and expression in mammalian
cells
JOURNAL Mol. Cell. Biol. 7, 725-737 (1987)
STANDARD full staff_review
REFERENCE 7 (sites)
AUTHORS Barnes,W.M.
TITLE ;
JOURNAL Unpublished (1991)
STANDARD full staff_review
REFERENCE 8 (bases 1 to 6082)
AUTHORS Barnes,W.M.
TITLE Variable patterns of expression of Luciferase in transgenic tobacco
leaves
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 9183-9187 (1991)
STANDARD simple staff_entry
COMMENT Sequence source are the following: bases 5-505 corresponds to
2596-2096 pTi T37 as numbered in [Bevan, et al.]; bases 646-1632 to
6492-7478 of CaMV1841 as numbered in [Gardner, et al.]J02046; bases
1698-3338 firefly luciferase codons 4 through 549 (last)
nucleotides 312-1952 of reference [De Wet, et al.]; bases 3346-4125
NPTII (kanamycin resistance) codons, bp 166-945 of reference [Beck,
et al.] J01834; bases 4125 1743 3' end of tomato protease inhibitor
I gene, bp 4125-4840 of reference [Lee, et al.] M13938; conflict
4335-4340 TAATAA here is TAA in [Lee, et al.]; bases 4855-5538 3'
end of nopaline synthase gene, bp 687-2 of reference [Bevan, et
al.]; bases 5544-5573 LacZ DNA, 1390-1419 of accession J01636;
bases 5572-6082 existing restriction sites [Barnes, et al.] in 10.2
kb of vector pRK252 [Ditta, et al.], not sequenced or mapped. The
sequence at the end of this entry, containing many restriction
sites spaced by arbitrary spans of 10 unknown nucleotides, is a
computer-readable representation of the restriction sites in vector
pRK252 [Ditta, et al.] DNA. pRK252 [Ditta, et al.] has been tested
and found to lack sites for the following restriction enzymes
(partial list [Barnes, et al.]): SacI, ClaI, HpaI, XbaI, KpnI,
PstI, ScaI, NsiI, HindIII, BamH1, AflII. The Luc/Kan gene is not
expressed in E.coli, but is expressed in Agrobacteri
FEATURES Location/Qualifiers
CDS 1660..4125
/codon_start=1660
/product="luciferase/kanamycin resistance protein"
BASE COUNT 1626 a 1301 c 1321 g 1496 t 338 others
ORIGIN
1 gaattcgagg atttttcggc gctgcgctac gtccgcgacc gcgttgaggg atcaagccac
61 agcagcccac tcgaccttct nnccgaccca gacgagccaa gggatctttt tggaatgctg
121 ctccgtcgtc aggctttccg acgtttgggt ggttgaacag aagtcattat cgcacggaat
181 gccaagcact cccgagggga accctgtggt tggcntgcac atacaaatgg acgaacggat
241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
361 atctgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
421 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
481 ggtttctgga gtttaatgag ctaagggtac cctaagcaca tacgtcagaa accattattg
541 cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct
601 ccactgacgt tccataaatt cccctcggta tccaattaga gtctcagatc tcctttgccc
661 cggagatcac catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag
721 aaggtgacga taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa
781 agaatgctga cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct
841 acccgagtaa taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca
901 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa
961 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga
1021 ttggagtctc taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga
1081 tcgaggatct aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac
1141 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact
1201 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
1261 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
1321 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
1381 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
1441 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
1501 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
1561 aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc
1621 tatctctctc tagatctctc tcaatccaaa taatctgcaa tggcaattac cggatcttgg
1681 aggttactta tggatcccgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta
1741 gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
1801 ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc
1861 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
1921 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
1981 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
2041 atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg
2101 aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat
2161 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
2221 gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat
2281 tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc
2341 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
2401 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
2461 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt
2521 caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa
2581 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca
2641 cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga
2701 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
2761 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
2821 accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt
2881 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
2941 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc
3001 ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata
3061 ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc
3121 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
3181 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
3241 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
3301 atcctcataa aggccaagaa gggcggaaag tccaaattct cgaggggatt gcacgcaggt
3361 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
3421 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
3481 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
3541 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
3601 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
3661 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
3721 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
3781 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
3841 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
3901 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
3961 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
4021 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
4081 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaagctt gctaaggaaa
4141 taattgagaa ggaaaatcca tccataacta atattccaat attattgagt ggttctccaa
4201 ttacattaga ttatctatgt gatcgagttc gtctttttga taacatcttg ggctttgttg
4261 tacaaatgcc tgtggtgact taattaatgg accttattat tgaagtaatt aagcagccac
4321 aatatgtcaa aaaataataa ttagggttca tgtatgttca ttataatgca tctcttcatg
4381 tactcttact atatataatg gaaaaaataa gtgtggctta attacatgta ctcctctcct
4441 taattaattc ctttttacat gtggtactac gttattttga ttattttaat ttatttgtct
4501 gattttgact ataacatctt tggacatata tttaattaat gtgcgagagc taagatttga
4561 attgtaattg acaaattagt cccccaaccc cactacaccc caaaaataaa aataaaacgt
4621 gacaatgtgt tataattttt tggaataaat taagaaggtt aagggatcaa gtaaacaaaa
4681 aatatgtgtg gaaagataaa tatgtattta tctttaatta gaagttttga atttgaacat
4741 aaatatatat aaaaaaaggt tatctattaa ttgatctact ctaaagtgga tattgaatat
4801 caaataaaaa actaaaaaaa agtaaagcat aacaagatct ctagaagctt ccgtcgtcga
4861 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
4921 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
4981 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
5041 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
5101 atctatgtta ctagatcgat caaacttcgg tactgtgtaa tgacgatgag caatcgagag
5161 gctgactaac aaaaggtatg cccaaaaaca acctctccaa actgtttcga attggaagtt
5221 tctgctcatg ccgacaggca taacttagat attcgcgggc tattcccact aattcgtcct
5281 gctggtttgc gccaagataa atcagtgcat ctccttacaa gttcctctgt cttgtgaaat
5341 gaactgctga ctgcccccca agaaagcctc ctcatctccc agttggcggc ggctgataca
5401 ccatcgaaaa cccacgtccg aacacttgat acatgtgcct gagaaatagg cctacgtcca
5461 agagcaagtc ctttctgtgc tcgtcggaaa ttcctctcct gtcagacggt cgtgcgcatg
5521 tcttgcgttg atgaagctag cttgctggcg taatagcgaa gaggcccgca ccggaattcn
5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttaaan nnnnnnnnnt gatcannnnn
5641 nnnnntgatc annnnnnnnn ncgatcgnnn nnnnnnncag ctgnnnnnnn nnncctnnnn
5701 naggnnnnnn nnnngtcgac nnnnnnnnnn cccgggnnnn nnncccgggn nnnnnnnnna
5761 ggcctnnnnn nnnnngaann nnttcnnnnn nnnnngcggc cgcnnnnnnn nnngcggccg
5821 cnnnnnnnnn ngcggccgcn nnnnnnnnng ccnnnnnggc nnnnnnnnnn gccnnnnngg
5881 cnnnnnnnnn ngccnnnnng gcnnnnnnnn nngccnnnnn ggcnnnnnnn nnngccnnnn
5941 nggcnnnnnn nnnnccgcgg nnnnnnnnnn ccgcggnnnn nnnnnnccgc ggnnnnnnnn
6001 nnaatattnn nnnnnnnnaa tattnnnnnn nnnnaatatt nnnnnnnnnn aatattnnnn
6061 nnnnnnaata ttnnnnnnnn nn
//GenBank-Updates@genbank.bio.net (02/13/91)
LOCUS PWB146 6082 bp ds-DNA SYN 13-FEB-1991
DEFINITION Artificial T-DNA conferring expression of a
luciferase/kanamycin-resistance fusion to transgenic plants.
ACCESSION M61152 M38354
KEYWORDS NPTII; firefly luciferase; kanamycin resistance; transfer DNA.
SOURCE Synthetic DNA.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (sites)
AUTHORS Ditta,G., Stanfield,S., Corbin,D. and Helinski,D.
TITLE Broad host range DNA cloning system for gram-negative bacteria:
Construction of a gene bank of Rhizobium meliloti
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77, 7347-7351 (1980)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Gardner,R.C., Howarth,A.J., Hahn,P., Brown-Luedi,M., Shepherd,R.J.
and Messing,J.
TITLE The complete nucleotide sequence of an infectious clone of
cauliflower mosaic virus by m13mp7 shotgun sequencing
JOURNAL Nucleic Acids Res. 9, 2871-2888 (1981)
STANDARD full staff_review
REFERENCE 3 (sites)
AUTHORS Beck,E., Ludwig,G., Auerswald,E.A., Reiss,B. and Schaller,H.
TITLE Nucleotide sequence and exact localization of the neomycin
phosphotransferase gene from transposon Tn5
JOURNAL Gene 19, 327-336 (1982)
STANDARD full staff_review
REFERENCE 4 (sites)
AUTHORS Bevan,M., Barnes,W.M. and Chilton,M.D.
TITLE Structure and transcription of the nopaline synthase gene region of
T-DNA
JOURNAL Nucleic Acids Res. 11, 369-385 (1983)
STANDARD full staff_review
REFERENCE 5 (sites)
AUTHORS Lee,J.S., Brown,W.E., Graham,J.S., Pearce,G., Fox,E.A.,
Dreher,T.W., Ahern,K.G., Pearson,G.D. and Ryan,C.A.
TITLE Molecular characterization and phylogenetic studies of a
wound-inducible proteinase inhibitor I gene in Lycopersicon species
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83, 7277-7281 (1986)
STANDARD full staff_review
REFERENCE 6 (sites)
AUTHORS de Wet,J.R., Wood,K.V., DeLuca,M., Helinski,D.R. and Subramani,S.
TITLE Firefly luciferase gene: Structure and expression in mammalian
cells
JOURNAL Mol. Cell. Biol. 7, 725-737 (1987)
STANDARD full staff_review
REFERENCE 7 (sites)
AUTHORS Barnes,W.M.
TITLE ;
JOURNAL Unpublished (1990)
STANDARD full staff_review
REFERENCE 8 (bases 1 to 6082)
AUTHORS Barnes,W.M.
TITLE Variable patterns of expression of Luciferase in transgenic tobacco
leaves
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 9183-9187 (1990)
STANDARD simple staff_entry
COMMENT Sequence source are the following: bases 5-505 corresponds to
2596-2096 pTi T37 as numbered in [Bevan, et al.]; bases 646-1632 to
6492-7478 of CaMV1841 as numbered in [Gardner, et al.]J02046; bases
1698-3338 firefly luciferase codons 4 through 549 (last)
nucleotides 312-1952 of reference [De Wet, et al.]; bases 3346-4125
NPTII (kanamycin resistance) codons, bp 166-945 of reference [Beck,
et al.] J01834; bases 4125 1743 3' end of tomato protease inhibitor
I gene, bp 4125-4840 of reference [Lee, et al.] M13938; conflict
4335-4340 TAATAA here is TAA in [Lee, et al.]; bases 4855-5538 3'
end of nopaline synthase gene, bp 687-2 of reference [Bevan, et
al.]; bases 5544-5573 LacZ DNA, 1390-1419 of accession J01636;
bases 5572-6082 existing restriction sites [Barnes, et al.] in 10.2
kb of vector pRK252 [Ditta, et al.], not sequenced or mapped. The
sequence at the end of this entry, containing many restriction
sites spaced by arbitrary spans of 10 unknown nucleotides, is a
computer-readable representation of the restriction sites in vector
pRK252 [Ditta, et al.] DNA. pRK252 [Ditta, et al.] has been tested
and found to lack sites for the following restriction enzymes
(partial list [Barnes, et al.]): SacI, ClaI, HpaI, XbaI, KpnI,
PstI, ScaI, NsiI, HindIII, BamH1, AflII. The Luc/Kan gene is not
expressed in E.coli, but is expressed in Agrobacterium
FEATURES Location/Qualifiers
CDS 1660..4125
/codon_start=1
/product="luciferase/kanamycin resistance protein"
BASE COUNT 1626 a 1301 c 1321 g 1496 t 338 others
ORIGIN
1 gaattcgagg atttttcggc gctgcgctac gtccgcgacc gcgttgaggg atcaagccac
61 agcagcccac tcgaccttct nnccgaccca gacgagccaa gggatctttt tggaatgctg
121 ctccgtcgtc aggctttccg acgtttgggt ggttgaacag aagtcattat cgcacggaat
181 gccaagcact cccgagggga accctgtggt tggcntgcac atacaaatgg acgaacggat
241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
361 atctgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
421 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
481 ggtttctgga gtttaatgag ctaagggtac cctaagcaca tacgtcagaa accattattg
541 cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct
601 ccactgacgt tccataaatt cccctcggta tccaattaga gtctcagatc tcctttgccc
661 cggagatcac catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag
721 aaggtgacga taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa
781 agaatgctga cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct
841 acccgagtaa taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca
901 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa
961 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga
1021 ttggagtctc taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga
1081 tcgaggatct aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac
1141 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact
1201 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
1261 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
1321 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
1381 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
1441 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
1501 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
1561 aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc
1621 tatctctctc tagatctctc tcaatccaaa taatctgcaa tggcaattac cggatcttgg
1681 aggttactta tggatcccgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta
1741 gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
1801 ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc
1861 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
1921 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
1981 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
2041 atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg
2101 aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat
2161 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
2221 gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat
2281 tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc
2341 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
2401 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
2461 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt
2521 caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa
2581 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca
2641 cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga
2701 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
2761 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
2821 accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt
2881 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
2941 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc
3001 ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata
3061 ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc
3121 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
3181 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
3241 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
3301 atcctcataa aggccaagaa gggcggaaag tccaaattct cgaggggatt gcacgcaggt
3361 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
3421 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
3481 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
3541 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
3601 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
3661 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
3721 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
3781 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
3841 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
3901 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
3961 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
4021 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
4081 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaagctt gctaaggaaa
4141 taattgagaa ggaaaatcca tccataacta atattccaat attattgagt ggttctccaa
4201 ttacattaga ttatctatgt gatcgagttc gtctttttga taacatcttg ggctttgttg
4261 tacaaatgcc tgtggtgact taattaatgg accttattat tgaagtaatt aagcagccac
4321 aatatgtcaa aaaataataa ttagggttca tgtatgttca ttataatgca tctcttcatg
4381 tactcttact atatataatg gaaaaaataa gtgtggctta attacatgta ctcctctcct
4441 taattaattc ctttttacat gtggtactac gttattttga ttattttaat ttatttgtct
4501 gattttgact ataacatctt tggacatata tttaattaat gtgcgagagc taagatttga
4561 attgtaattg acaaattagt cccccaaccc cactacaccc caaaaataaa aataaaacgt
4621 gacaatgtgt tataattttt tggaataaat taagaaggtt aagggatcaa gtaaacaaaa
4681 aatatgtgtg gaaagataaa tatgtattta tctttaatta gaagttttga atttgaacat
4741 aaatatatat aaaaaaaggt tatctattaa ttgatctact ctaaagtgga tattgaatat
4801 caaataaaaa actaaaaaaa agtaaagcat aacaagatct ctagaagctt ccgtcgtcga
4861 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
4921 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
4981 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
5041 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
5101 atctatgtta ctagatcgat caaacttcgg tactgtgtaa tgacgatgag caatcgagag
5161 gctgactaac aaaaggtatg cccaaaaaca acctctccaa actgtttcga attggaagtt
5221 tctgctcatg ccgacaggca taacttagat attcgcgggc tattcccact aattcgtcct
5281 gctggtttgc gccaagataa atcagtgcat ctccttacaa gttcctctgt cttgtgaaat
5341 gaactgctga ctgcccccca agaaagcctc ctcatctccc agttggcggc ggctgataca
5401 ccatcgaaaa cccacgtccg aacacttgat acatgtgcct gagaaatagg cctacgtcca
5461 agagcaagtc ctttctgtgc tcgtcggaaa ttcctctcct gtcagacggt cgtgcgcatg
5521 tcttgcgttg atgaagctag cttgctggcg taatagcgaa gaggcccgca ccggaattcn
5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttaaan nnnnnnnnnt gatcannnnn
5641 nnnnntgatc annnnnnnnn ncgatcgnnn nnnnnnncag ctgnnnnnnn nnncctnnnn
5701 naggnnnnnn nnnngtcgac nnnnnnnnnn cccgggnnnn nnncccgggn nnnnnnnnna
5761 ggcctnnnnn nnnnngaann nnttcnnnnn nnnnngcggc cgcnnnnnnn nnngcggccg
5821 cnnnnnnnnn ngcggccgcn nnnnnnnnng ccnnnnnggc nnnnnnnnnn gccnnnnngg
5881 cnnnnnnnnn ngccnnnnng gcnnnnnnnn nngccnnnnn ggcnnnnnnn nnngccnnnn
5941 nggcnnnnnn nnnnccgcgg nnnnnnnnnn ccgcggnnnn nnnnnnccgc ggnnnnnnnn
6001 nnaatattnn nnnnnnnnaa tattnnnnnn nnnnaatatt nnnnnnnnnn aatattnnnn
6061 nnnnnnaata ttnnnnnnnn nn
//