GenBank-Updates@genbank.bio.net (02/19/91)
LOCUS ASNACAINA 2633 bp ds-DNA BCT 19-FEB-1991
DEFINITION A.nidulans acyl-coenzyme A:Isopenicillin N acyltransferase (penDE)
gene, complete cds.
ACCESSION M58293
KEYWORDS acyl-coenzyme A:Isopenicillin N acyltransferase; acyltransferase.
SOURCE A.nidulans DNA.
ORGANISM Aspergillus nidulans
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 1 to 2633)
AUTHORS Tobin,M.B., Fleming,M.D., Skatrud,P.L. and Miller,J.R.
TITLE Molecular characterization of the acyl-coenzyme A:Isopenicillin N
acyltransferase gene (penDE) from Penicillium chrysogenum and
Aspergillus nidulans and activity of recombinant enzyme in
Escherichia coli
JOURNAL J. Bacteriol. 172, 5908-5914 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS join(431..466,520..694,750..901,961..1671)
/product="acyl-coenzyme A:Isopenicillin N
acyltransferase"
/gene="penDE"
/codon_start=431
exon 301..466
/number=1
/gene="penDE"
exon 520..694
/number=2
/gene="penDE"
exon 750..901
/number=3
/gene="penDE"
exon 961..1740
/number=4
/gene="penDE"
mRNA join(301..466,520..694,750..901,961..1740)
/gene="penDE"
BASE COUNT 685 a 700 c 595 g 653 t
ORIGIN
1 attgcacagg cccttttcgc tccacccgca gcgccacgaa tccggttggc atcggcgcga
61 tgtcacctgc aatggaacgg cgataggatc ttgatgctca ccagccaatc acaggttctc
121 caagaagcac tcgggatcca cgggttccgt ctcagtagcg gctgcgtaca cgatcctaac
181 ctaaaagaac cacaggccaa gcccacatgc atagcatgtt gatttccctg gcccggcgcc
241 ggccgaacct tggtctaggg ctttgtgcgc tctgtcgatt ccgtgtcaaa actggaccag
301 gcagcaggaa catgtctgta ttctagatct catcggtact tgccgccaac tcacccaaat
361 cgcgatctcg aatgcaccta tctagctagc caccctgttc aatattttct actggacgct
421 ccgaagaaaa atgcttcacg taacttgcca aggtaccccc tccgaagtaa gtccataccc
481 gaatgtatat ttgcccatat ttaacgccta tacttccaga tcggctatca ccatggctct
541 gctgccaaag gcgagattgc gaaagccatt gacttcgcaa ctggcctcat tcatggcaaa
601 acaaaaaaga cacaggcgga gcttgaacag ctcctcaggg agttggagca ggtgatgaaa
661 cagcgctggc cgagatacta tgaggaaatc tgcggtatgt ttacctacgt tctttacttg
721 ctgaatcacc ccgttgacgg aattttcagg aatcgcaaag ggtgcggaac gcgaagtatc
781 ggagattgtc atgctcaaca ctcgtacgga attcgcgtac gggctcgtag aagcccggga
841 cgggtgcacc actgtttact gcaaaacccc caatggagcg ctacagggcc agaactggga
901 cgtaggttat tagatccacg gtctgctatc tattttcctt gctaaccgcc attccggcag
961 ttcttcaccg caaccaaaga aaacttgatc cagttaacaa tttgtcagcc gggtctaccc
1021 actatcaaaa tgattacaga agctggtatc attggcaaag tgggtttcaa cagtgctggt
1081 gtcgctgtca attacaatgc actacaccta catggcctcc gtcccactgg cctcccctcg
1141 catctcgcgc tgcgcatggc cctcgaaagt acatctccgt ctgaggcgta tgaaaaaatc
1201 gtctcgcaag ggggcatggc ggctagcgcg ttcatcatgg tgggcaacgc acacgaggcc
1261 tacgggctag agttctcgcc catcagcttg tgcaagcaag ttgctgacac caatgggcgg
1321 atagtgcata cgaaccactg cctcctcaac catgggccat cggcgcaaga gcttaatccc
1381 ctgccggact cgtggagccg ccacgggcgg atggaacatc tcctctctgg ttttgacggc
1441 acgaaggagg catttgcgaa gttgtgggag gacgaagaca actaccctct ctcgatctgc
1501 cgggcatata aggaagggaa aagtagaggc tccactcttt tcaacatcgt cttcgatcat
1561 gtgggccgga aggcaacagt gcggctgggc cggcccaata accctgatga gacctttgtc
1621 atgaccttta gcaatctgga taccaagtcc gcgatccaag ccaacatttg accaatatct
1681 ctttccaacc gatgccttct gcagtcttct ccagccatag cgtactacac cgtgaacact
1741 tctatgtatc ttgacaacta gatacaagtc aaaaggcgaa tcaaataaag tcgcatgttt
1801 gggagaatgc acgccagagt gaaatgctga atcctgttgg ctttcacaac caggtgccta
1861 aagtatagca atgcaacgaa ggcatgccta gtctagcttc tttccctaaa catgctttaa
1921 atctaatacg agaattattt aaagcattcc agaactacta ctaacttcct attaccctgg
1981 agtttcctgt ttcggaaggg ttgacctctc aatccttcct aaccttgtga gtctagagta
2041 agctctgcaa tcttgtttaa cctacctagg actcacgaag actccctcag tatgaagttg
2101 ataaaggttc tttgtgctcc gagcccgcaa gggaaatcag tgctgagata tactggctgg
2161 gctctatatg ttgtagagtc aagtgttgac aatttgaatc ctcgctaatg tcacggctct
2221 gcaagccggg atcccgagtt ggtaaagcat gcaacagcaa gccccatttt atctacatat
2281 tagctacgat tatcctaatc attgctttta gaataatctc taaatttgga gccagcaggt
2341 cacagagctc actctaggca ccccgggcac agtgctcgaa atagcgtcgt gccgttatcc
2401 agcttcatac attggtgtaa aagaagaatc gagccatttc tggccaccca tctgataaag
2461 ttaggcctag ctccgtgaag acattccaca aaaatctctc catgaacata attcttctca
2521 gtgcttagtt tcttgaacat gagctacaga atggtttttt gaatatttca attggctttc
2581 caccataata tatgattata gttactaatt agactacttc tatagcaaag ctt
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