GenBank-Updates@genbank.bio.net (02/19/91)
LOCUS YSGMEL 3612 bp ds-DNA PLN 19-FEB-1991
DEFINITION S.carlsbergensis alpha-galactosidase (MEL) gene, complete cds.
ACCESSION M58484
KEYWORDS alpha-galactosidase; melibiase.
SOURCE S.carlsbergensis (strain NCYC396) DNA.
ORGANISM Saccharomyces carlsbergensis
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 3612)
AUTHORS Turakainen,H., Korhola,M. and Aho,S.
TITLE Cloning, sequence and chromosomal location of a MEL gene from
Saccharomyces carlsbergensis NCYC396
JOURNAL Unpublished (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
protein_bind 1156..1172
/note="GAL4 protein binding site."
TATA_signal 1311..1317
/gene="MEL"
CDS 1391..2806
/EC_number="3.2.1.22"
/product="alpha-galactosidase"
/gene="MEL"
/codon_start=1391
terminator 2829..2866
/gene="MEL"
BASE COUNT 1117 a 676 c 714 g 1105 t
ORIGIN Chromosome X.
1 gaattcgaga agactgaaaa attaaagtgt tcaaacccta tgttcactaa caactaacta
61 aatctatata ttggtcatct tagagattac agtacctaga gtactcgaga tatttcaaaa
121 tattcatgac acctgagaca actaaccaga tcatatcgat atatcagaag attttctcac
181 gccatgcccg ttttgattga gcaccttatt tatacttcct gttcactaaa aaatacctta
241 aactctcaca aatgaattat ttgaaatatt gaaccacatc gcaatcaata caatatcaaa
301 agtacacatc tcttcacaag gtttctctag cacgtataat acggcttggt tgtaaaggaa
361 tacactgcaa aatttttctc aaaaatgctt ttttcatagt cttttagttg cggtttagat
421 ctcggtattg ccaagtactt caacagatcc accactgccc tactctttac cgatggtgaa
481 ggaacctcaa tactgaaaca ttggttctgc tgagagaata aaataaaaaa ttgattagta
541 ttgggaaaat actactccaa gactattaca tctgtatgcc tgttatcgca tagatcaaag
601 agtggagtaa tttgaaaagt acagaaagcg gaaattgaat ttttcaaatc attgagttat
661 ttttttagaa ctagttaaga gtgctgatgg gacattgtaa caacattttt cccacaaacc
721 ttccgattca tcatattgcg gggaatatta tgatatagta aggcaaaggg gacacgttta
781 gataggtacc agattctgtt ttaaacgacg ctactaatct cttttattag atgaattacc
841 atcaaaggat cgtggggtat cactcaacat acaccgtctg ctattcattt cattcaatgc
901 accctagctc cctactccca acaatgttaa acccctacga accctgacaa ggcggatcgg
961 agacgaataa ttctgaaaca tactccagag ctccgaactg accctttttg aactcaacac
1021 aaaaattaac cgcagttaaa gctgataatg attatgtgtt tgttaggata tcaaagatct
1081 tgtgcttaga cattctcgtt aaggttttag ttgggacccc ttagacccct cccgaaatac
1141 gacagtccat tacttcggcc tagtgttgtc cgcgacgggg caaaattaaa atgcctaacc
1201 tgaaatgcct tctctatact acatgatgaa atattatgaa ccataggatg aaaggtagct
1261 gatgaaggtt attaaaatga taagatagga tgaagctagc tgatgaaggt tataaaaatg
1321 gagataagat taatgataaa attcaagcgg tatacagttg ttgtttgttc ctagtgaaaa
1381 aaaagtatca atgtttcttt tatatctttt tacctctttc gccgctgtga gcggtgttct
1441 cggttcgtca ccgagttaca atggccttgg cctcacccct caaatgggtt gggacaattg
1501 gaacacattt gcctgcgacg tcagtgagca attgcttttg gataccgctg accggatttc
1561 tgaaatagga ctaaaggatt tgggttacac ttatgttata ttggatgact gctggtctag
1621 tggcagaact gcaaatggta cactcgttgc agacaaggaa aagtttccca atggtatgag
1681 tcatgtggct gaccacctgc ataacaacaa ttttctcttt ggcatgtact catctgctgg
1741 tgagtacact tgtgcaggat accctggatc tttaggacac gaggaagaag atgcggagtt
1801 ttttgctagc aatggtgttg attacttaaa gtacgacaac tgttacaaca aaggtcagtt
1861 tggtgcacca gaaacttcct acaaacgtta caaggctatg tctgatgctt tgaacaaaac
1921 aggcagacct atattttatt ctttgtgtaa ctggggtcaa gatttgacgc attattgggg
1981 gtctgatata gcaaactcct ggagaatgag tggggatatt tatcctcagt ttactcgtcc
2041 tgacagtagg tgtccttgcg atggtgacca gtttgattgc gcatatgctg gttttcattg
2101 ttctatcatg aatattctaa acaaagcagc tccaatgggg caaaatgcgg gaataggtgg
2161 ctggaacgat ctggataact tagaagtcgg tgtcggaaac ttgacagatg atgaagagaa
2221 agcgcatttc tctatgtggg caatggttaa gtctccacta gtcatcggcg ctgatgtgaa
2281 tcacttaaaa gcgtcttcat attcaattta cagtcaagct tccgtaatag caatcaacca
2341 ggatcccaag ggagtaccag caacaagagt ttggagacat caagtgccac aaactgacaa
2401 gtatggtcaa ggtgaaattc aattttggag cggtccactt gataacgggg atcaagttat
2461 tgctctctta aatgggggaa tcaaaccaag accaatgaat acgaacttgg aagaaatttt
2521 ctttgacagc tacttaggtt tcgagcaatt gtcctcaaac tgggatattt atgacttatg
2581 ggctaacaga gttgacaatg caacatcggc taacattctg aataataaca gcgtaggaaa
2641 tgctactatt tacaatgcta ccgcactatc atacaaggat gggatggcaa agaatgatac
2701 cagattattc ggtaccaaaa tcggcagtat ttctccagat ggcttgctta acacaactgt
2761 tccagcacac ggaattgctt tctataggtt aagacggtct acttaataag aagatttcca
2821 tcaaccgggt aggttttttc ttcagattag tttaaagggt acatttttta tacgttatgc
2881 aaagcgtttt gaaattttta ccagcaagtg acgagacgac agtatttata tctttacaga
2941 cgcaatcacg ggtagcgccg catggttaag agtgcccgaa tttattaaat cagtcttagt
3001 ataatgatgg gtctaagtta taatagacta tgttgcaagt gccaaaataa cacctttttg
3061 agaaaattaa tttccctgcg gtttttaaac cataaagtat cgtttgaatt gttagcaaat
3121 ctggcctacg tgagagttat caaacactat tggtagtgtc cgcaaaaaac ttttctcttg
3181 tgaatgatgc tcccctaaca tcatggttgc ttgacgtgaa agtatgataa aagaagattc
3241 attgtaagcg ctagaataac tcaatagctc ttccttgtta tttcagcttt gggttttttt
3301 tttaagttgt gcaacagacc gatcatattc aattttcatt tattcatatt tagctcgaaa
3361 aaataaaatt aatttcaggt gatattagta gttcaactgc aatgaagtct gtgttccaaa
3421 ccgcgtaaat ttagtatata tatatatata tatctatcaa ttacaataca gtgtggacaa
3481 cttcaaaaca cacatgccct ggtatgtcct tggtttctgc tagttccaaa acaacttgcc
3541 tactgactcg atggcggatt tttcgttatg gaatcgcctt atttgtttct agcggtattt
3601 gccttggaag ct
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