GenBank-Updates@genbank.bio.net (02/20/91)
LOCUS ECOPGSA 955 bp ds-DNA BCT 20-FEB-1991
DEFINITION E.coli pgsA and glyW genes encoding phosphatidylglycerophosphate
synthase and Gly-tRNA-3, complete cds, and uvrC gene, 3' end.
ACCESSION M12299
KEYWORDS glyW gene; pgsA gene; phosphatidylglycerophosphate synthetase;
synthetase; transfer RNA; uvrC gene.
SOURCE E.coli K12 DNA, clones pPG1 and pPGL2019.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 955)
AUTHORS Gopalakrishnan,A.S., Chen,Y.-., Temkin,M. and Dowhan,W.
TITLE Structure and expression of the gene locus encoding the
phosphatidylglycerophosphate synthase of Escherichia coli
JOURNAL J. Biol. Chem. 261, 1329-1338 (1986)
STANDARD full staff_review
COMMENT Draft entry and computer readable sequence in [J. Biol. Chem. 261,
1329-1338 (1986)] were kindly
provided by W. Dowhan, 06-APR-1986.
FEATURES Location/Qualifiers
CDS <1..25
/note="uvrC gene (AA at 2)"
/codon_start=2
mRNA 49..>732
/note="pgsA mRNA"
CDS 82..732
/note="phosphatidylglycerophosphate synthase (pgsA; EC
2.7.8.7)"
/codon_start=82
tRNA 781..856
/note="Gly-tRNA-3"
BASE COUNT 207 a 213 c 263 g 272 t
ORIGIN BglII site; 42.0 min on K12 map.
1 gatcttctgg tcgttgaaac attgatgtct ctgtagcaac ataggggtaa tcttactgac
61 aacagatagt tacccgtcat tatgcaattt aatatcccta cgttgcttac actgttccgt
121 gtcatcctta tcccattctt tgtattggtc ttttatctgc ctgtcacctg gtcgccgttt
181 gccgccgcgc tcattttctg cgtcgcggcg gtgactgact ggttcgatgg ttttctggca
241 cgccgctgga accagagtac ccggtttggt gctttccttg accctgtggc agataaagtt
301 ctcgtggcta tcgccatggt gctggtaacc gagcattatc acagctggtg ggtgacatta
361 ccggcggcaa cgatgatcgc ccgtgaaatt attatttctg cgctacgcga atggatggcg
421 gagttgggta aacgcagtag cgtggccgtc tcttggattg ggaaagtgaa aaccactgcc
481 cagatggtgg cgttggcatg gctgctgtgg cgtccgaaca tttgggttga gtacgccggt
541 attgcacttt tctttgtggc tgcggtactg actctgtggt caatgttgca atatttgagc
601 gctgcgcggc agatttgctt gatcagtgat cgtttcggcg taattttcag caaacgatca
661 aaagtggtga aaaatatcgt tgactcatcg cgccaggtaa gtagaatgca acgcatcgaa
721 cggcggcact gattgccaga cgataataaa atcaagtgat taactgattg cttgatgaat
781 gcgggaatag ctcagttggt agagcacgac cttgccaagg tcggggtcgc gagttcgagt
841 ctcgtttccc gctccagttt aaaagacatc ggcgtcaagc ggatgtctgg ctgaaaggcc
901 tgaagaattt ggcgcgttaa caaagcggtt atgtagcgga ttgcaaatcc gtcta
//