GenBank-Updates@genbank.bio.net (02/20/91)
LOCUS ECODNAZX 5239 bp ds-DNA BCT 20-FEB-1991
DEFINITION E. coli dnaZX replication gene.
ACCESSION X04275 M17218 M37084
KEYWORDS DNA polymerase III; adenylate kinase; apt protein; dnaZX gene; heat
shock protein C62.5; recR protein.
SOURCE E.coli (K12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 5239)
AUTHORS Yin,K.-C., Blinkowa,A. and Walker,J.R.
TITLE Nucleotide sequence of the Escherichia coli replication gene dnaZX
JOURNAL Nucleic Acids Res. 14, 6541-6549 (1986)
STANDARD simple automatic
REFERENCE 2 (sites)
AUTHORS Bardwell,J.C. and Craig,E.A.
TITLE Eukaryotic Mr 83,000 heat shock protein has a homologue in
Escherichia coli
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 84, 5177-5181 (1987)
STANDARD full staff_review
REFERENCE 3 (sites)
AUTHORS Yeung,T., Mullin,D.A., Chen,K.-S., Craig,E.A., Bardwell,J.C. and
Walker,J.R.
TITLE Sequence and expression of the Escherichia coli recR locus
JOURNAL J. Bacteriol. (1990) In press
STANDARD full staff_review
COMMENT The dnaZX gene codes for two proteins of different molecular
weight: the X protein (Mw 75000-83000 d) and the Z protein (Mw
52000-56500 d); the mechanism by which the two proteins are
generated is unknown. The proteins are probably subunit tau(X)
and
gamma(Z) of DNA polymerase III
EMBL features not translated to GenBank features:
key from to description
PRM 70 75 pot. -35 region
PRM 93 98 pot. -10 region
RBS 131 134 pot. rRNA binding site
SITE 256 318 pot. adenine nucleotide binding
site
The gene encoding C62.5 (htpG) is located between the dnaZ and adk
genes.
Draft entry and computer-readable sequence for [J. Bacteriol.
(1990) In press] kindly submitted
by J.R.Walker, 25-JUL-1990.
FEATURES Location/Qualifiers
CDS <1..13
/note="apt protein (C-term.; AA at 2)"
/codon_start=2
CDS 142..2073
/note="dnaZX gene product (aa 1-643)"
/codon_start=142
misc_signal 1892..1897
/note="-35 region"
misc_signal 1915..1920
/note="-10 region"
misc_binding 2114..2119
/note="ribosome binding site"
CDS 2126..2455
/note="ORF 12"
/codon_start=2126
CDS 2455..3060
/note="recR product"
/codon_start=2455
CDS 3170..5044
/note="heat shock protein C62.5"
/codon_start=3170
misc_signal 5048..5079
/note="terminator (pot.)"
CDS 5225..>5239
/note="adenylate kinase (EC 2.7.4.3)"
/codon_start=5225
BASE COUNT 1271 a 1403 c 1464 g 1101 t
ORIGIN
1 cccgggccat taattatcgc cagtcttgtg ctgcccacgc tacggacagc acaagatgtg
61 cattcagcct cgccgttctg acggggctgt gttagcatta ccccttcgtg aatccacctt
121 ccagcgtttc agagcctgcc aatgagttat caggtcttag cccgaaaatg gcgcccacaa
181 acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg
241 ttagggcgta ttcatcatgc ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct
301 atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc
361 ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc
421 gacgccgcct cgcgcaccaa agttgaagat acccgcgacc tgctggataa cgtccagtac
481 gctccggcgc gtggtcgttt caaagtttat ctgatcgacg aagtgcatat gctgtcgcgc
541 cacagcttta acgcactgtt aaaaaccctt gaagagccgc cggagcacgt taagtttctg
601 ctggcgacga ccgatccaca gaaattgccg gtgacgattt tgtcacgctg tctgcaattt
661 catctcaagg cgctggatgt cgagcaaatt cgccatcagc ttgagcacat cctcaacgaa
721 gaacatatcg ctcacgagcc gcgggcgctg caattgctgg cacgcgccgc tgaaggcagc
781 ctgcgagatg ccttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca
841 acccaggcgg tcagtgcgat gctgggtacg cttgacgacg atcaggcgct gtcgctggtt
901 gaagcgatgg tcgaggccaa cggcgagcgc gtaatggcgc tgattaatga agccgctgcc
961 cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg gcctgttgca tcgtattgcg
1021 atggtacaac tttcgcctgc tgcacttggc aacgacatgg ccgccatcga gctgcggatg
1081 cgtgaactgg cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg
1141 attggtcgca aagaattacc gtatgcgccg gaccgtcgca tgggcgttga gatgacgctg
1201 ctgcgcgcgc tggcattcca tccgcgtatg ccgctgcctg agccagaagt gccacgacag
1261 tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa cccaggtgcc gccgcaaccg
1321 caatcagcgc cgcagcaggc accgactgta ccgctcccgg aaaccaccag ccaggtgctg
1381 gcggcgcgcc agcagttgca gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg
1441 gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc tggaaagact ggcttcggtc
1501 accgatcgcg ttcaggcgcg tccggtgcca tcggcgctgg aaaaagcgcc agccaaaaaa
1561 gaagcgtatc gctggaaggc gaccactccg gtgatgcagc aaaaagaagt ggtcgccacg
1621 ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagcta
1681 gcggcagaag ccattgagcg cgacccgtgg gcggcacagg tgagccaact ttcgctacca
1741 aaactggtcg aacaggtggc gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt
1801 ctgcatttgc gctcctctca gcggcatttg aacaaccgcg gtgcacagca aaaactggct
1861 gaagcgttga gcatgttaaa aggttcaacg gttgaactga ctatcgttga agatgataat
1921 cccgcggtgc gtacgccgct ggagtggcgt caggcgatat acgaagaaaa acttgcgcag
1981 gcgcgcgagt ccattattgc ggataataat attcagaccc tgcgtcggtt cttcgatgcg
2041 gagctggatg aagaaagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc
2101 cttaacgtga ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa
2161 gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga
2221 agtcaccggc gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacacaactg
2281 ccgtcgcgca gagatcgacc cgagcctgct ggaagacgac aaagagatgc tggaagacct
2341 ggtggctgca gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat
2401 ggcctctgta tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa
2461 accagcccgc tgttaacaca gcttatggaa gcactgcgct gtctgccggg cgttggcccg
2521 aagtcggcgc agcgtatggc gttcaccgtg cttcagcgcg atcgtagcgg cgggatgcgt
2581 ctggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc actgcgccga ttgccgcact
2641 ttcaccgaac aggaagtctg taacatctgt tcgaatccgc gtcgtcagga aaacggtcaa
2701 atctgcgtgg tggagagtcc ggcggacatc tacgccattg agcagacggg gcagttttca
2761 ggtcgttatt ttgtgttgat ggggcatctg tcaccgctgg acggcatcgg tccggatgat
2821 atcgggcttg atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc
2881 gccaccaacc ccacggttga aggtgaagct accgctaact acattgccga gctttgcgcg
2941 caatatgacg tggaagccag ccgaatcgct catggcgttc cggttggcgg cgagctggaa
3001 atggtcgacg gcaccacgtt gtcacactcc cttgccgggc gtcataagat tcgtttttaa
3061 gcaaacgaga gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct
3121 cccacatcct gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca
3181 agaaactcgt ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct
3241 ctattccaat aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga
3301 caagctgcgt ttccgtgcgc tctctaaccc ggacctgtac gaaggtgatg gcgaactacg
3361 cgttcgtgtc tctttcgata aagacaagcg tacgctgacc atctccgata acggcgtggg
3421 gatgacccgc gacgaagtga ttgaccatct ggggactatc gctaaatccg gtaccaaatc
3481 attcctcgaa tccctgggtt ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg
3541 tgttggtttc tactctgcgt ttatcgtggc cgacaaagtg accgtgcgta ctcgcgcggc
3601 aggcgaaaaa ccagaaaatg gcgtcttctg ggaatcggct ggcgaaggtg aatacaccgt
3661 tgccgacatc accaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga
3721 agacgagttc ctcgatgact ggcgcgtgcg ttccatcatc agcaaatact ccgaccatat
3781 cgcgctgccg gtagagatcg aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg
3841 ggagaaaatc aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga
3901 agagtacaaa gagttctaca aacacatcgc ccacgacttt aatgatccgc tgacctggag
3961 ccacaaccgt gttgaaggta agcaggagta caccagcctg ctgtacatcc cgtcccaggc
4021 tccgtgggat atgtggaacc gcgatcataa acacggcctg aaactgtatg ttcagcgtgt
4081 gttcatcatg gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct
4141 gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt
4201 aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc
4261 gaaagacgac gcggaaaaat accagacctt ctggcaacag tttggcctgg tactgaaaga
4321 aggtccggcg gaagatttcg ctaaccagga agcgatcgcc aaactgctgc gttttgcttc
4381 tacccatacc gattcttctg cgcagaccgt atctctggaa gactacgttt cccgcatgaa
4441 agaagggcag gagaaaatct actacatcac cgcagacagc tatgcggcag cgaagagcag
4501 cccgcacctg gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat
4561 cgatgagtgg atgatgaact atctgactga gttcgacggt aaaccgttcc agtcggtgtc
4621 taaagttgac gagtcgcttg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc
4681 ggagaaagca ctgactccgt tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa
4741 agatgtccgt ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga
4801 cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt
4861 gaaatacatc ttcgaactga acccggatca cgtactggtg aaacgtgcgg cagatactga
4921 agatgaagcg aagttcagcg agtgggtaga actgctgctg gatcaggcgc tgctggcaga
4981 acgcggcacg ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc
5041 ctgatgtaat gccggatgac cttcgtgtca tccggcattt ttcttttcat catctgcact
5101 ttccgcaaat tatctcgcca ttaaccgttt cagccccagg tgcctttctt gaggcaatcg
5161 cctgttggtg gtatcgttta tcgctttttc aaaaaattcg acacatttta aggggatttt
5221 cgcaatgcgt atcattctg
//