GenBank-Updates@genbank.bio.net (02/21/91)
LOCUS BTHCRYIA 4320 bp ds-DNA BCT 21-FEB-1991
DEFINITION B.thuringiensis delta-endotoxin gene, complete cds.
ACCESSION M35524
KEYWORDS delta-endotoxin.
SOURCE B.thuringiensis kenyae (strain HD588) DNA.
ORGANISM Bacillus thuringiensis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4320)
AUTHORS Von Tersch,M.A., Robbins,H.L., Jany,C.S. and Johnson,T.B.
TITLE Insecticidal toxins from Bacillus thuringiensis subsp. kenyae: Gene
cloning and characterization and comparison with B. thuringiensis
subsp. kurstaki CryIA(c) toxins
JOURNAL Appl. Environ. Microbiol. 57, 349-358 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable [or printed] sequence for
[Unpublished (1990)]
kindly submitted by M.A.Von Tersch, 19-JUN-1990.
Author address: M.A.Von Tersch
Ecogen Inc.
2005 Cabot Blvd. West
Loanghorne PA 19047
FEATURES Location/Qualifiers
misc_binding 228..232
/note="ribosome binding site"
CDS 239..3772
/note="delta-endotoxin"
/codon_start=239
BASE COUNT 1392 a 724 c 909 g 1295 t
ORIGIN
1 gttaacggaa tacaaaccct taatgcattg gttaaacatt gtaaagtcta aagcatggat
61 aatgggcgag aagtaagtag attgttaaca ccctgggtca aaaattgata tttagtaaaa
121 ttagttgcac tttgtgcatt ttttcataag atgagtcata tgttttaaat tgtagtaatg
181 aaaaacagta ttatatcata atgaattggt atcttaataa aagagatgga ggtaacttat
241 ggataacaat ccgaacatca atgaatgcat tccttataat tgtttaagta accctgaagt
301 agaagtatta ggtggagaaa gaatagaaac tggttacacc ccaatcgata tttccttgtc
361 gctaacgcaa tttcttttga gtgaatttgt tcccggtgct ggatttgtgt taggactagt
421 tgatataata tggggaattt ttggtccctc tcaatgggac gcatttcttg tacaaattga
481 acagttaatt aaccaaagaa tagaagaatt cgctaggaac caagccattt ctagattaga
541 aggactaagc aatctttatc aaatttacgc agaatctttt agagagtggg aagcagatcc
601 tactaatcca gcattaagag aagagatgcg tattcaattc aatgacatga acagtgccct
661 tacaaccgct attcctcttt tggcagttca aaattatcaa gttcctcttt tatcagtata
721 tgttcaagct gcaaatttac atttatcagt tttgagagat gtttcagtgt ttggacaaag
781 gtggggattt gatgccgcga ctatcaatag tcgttataat gatttaacta ggcttattgg
841 caactataca gatcatgctg tacgctggta caatacggga ttagaacgtg tatggggacc
901 ggattctaga gattgggtaa ggtataatca atttagaaga gaattaacac taactgtatt
961 agatatcgtt gctctgttcc cgaattatga tagtagaaga tatccaattc gaacagtttc
1021 ccaattaaca agagaaattt atacaaaccc agtattagaa aattttgatg gtagttttcg
1081 aggctcggct cagggcatag aaagaagtat taggagtcca catttgatgg atatacttaa
1141 cagtataacc atctatacgg atgctcatag gggttattat tattggtcag ggcatcaaat
1201 aatggcttct cctgtcggtt tttcggggcc agaattcacg tttccgctat atggaaccat
1261 gggaaatgca gctccacaac aacgtattgt tgctcaacta ggtcagggcg tgtatagaac
1321 attatcctct actttttata gaagaccttt taatataggg ataaataatc aacaactatc
1381 tgttcttgac gggacagaat ttgcttatgg aacctcctca aatttgccat ccgctgtata
1441 cagaaaaagc ggaacggtag attcgctgga tgaaatacca ccacagaata acaacgtgcc
1501 acctaggcaa ggatttagtc atcgattaag ccatgtttca atgtttcgtt caggctctag
1561 tagtagtgta agtataataa gagctcctat gttctcttgg atacatcgta gtgctgaatt
1621 taataatata attgcatcgg atagtattac tcaaatccct gcagtgaagg gaaactttct
1681 ttttaatggt tctgtaattt caggaccagg atttactggt ggggacttag ttagattaaa
1741 tagtagtgga aataacattc agaatagagg gtatattgaa gttccaattc acttcccatc
1801 gacatctacc agatatcgag ttcgtgtacg gtatgcttct gtaaccccga ttcacctcaa
1861 cgttaattgg ggtaattcat ccattttttc caatacagta ccagctacag ctacgtcatt
1921 agataatcta caatcaagtg attttggtta ttttgaaagt gccaatgctt ttacatcttc
1981 attaggtaat atagtaggtg ttagaaattt tagtgggact gcaggagtga taatagacag
2041 atttgaattt attccagtta ctgcaacact cgaggctgaa tataatctgg aaagagcgca
2101 gaaggcggtg aatgcgctgt ttacgtctac aaaccaacta gggctaaaaa caaatgtaac
2161 ggattatcat attgatcaag tgtccaattt agttacgtgt ttatcggatg aattttgtct
2221 ggatgaaaag cgagaattgt ccgagaaagt caaacatgcg aagcgactca gtgatgaacg
2281 caatttactc caagattcaa atttcaaaga cattaatagg caaccagaac gtgggtgggg
2341 cggaagtaca gggattacca tccaaggagg ggatgacgta tttaaagaaa attacgtcac
2401 actatcaggt acctttgatg agtgctatcc aacatatttg tatcaaaaaa tcgatgaatc
2461 aaaattaaaa gcctttaccc gttatcaatt aagagggtat atcgaagata gtcaagactt
2521 agaaatctat ttaattcgct acaatgcaaa acatgaaaca gtaaatgtgc caggtacggg
2581 ttccttatgg ccgctttcag cccaaagtcc aatcggaaag tgtggagagc cgaatcgatt
2641 cgcgccacac cttgaatgga atcctgactt agattgttcg tgtagggatg gagaaaagtg
2701 tgcccatcat tcgcatcatt tctccttaga cattgatgta ggatgtacag acttaaatga
2761 ggacctaggt gtatgggtga tctttaagat taagacgcaa gatgggcacg caagactagg
2821 gaatctagag tttctcgaag agaaaccatt agtaggagaa gcgctagctc gtgtgaaaag
2881 agcggagaaa aaatggagag acaaacgtga aaaattggaa tgggaaacaa atatcgttta
2941 taaagaggca aaagaatctg tagatgcttt atttgtaaac tctcaatatg atcaattaca
3001 agcggatacg aatattgcca tgattcatgc ggcagataaa cgtgttcata gcattcgaga
3061 agcttatctg cctgagctgt ctgtgattcc gggtgtcaat gcggctattt ttgaagaatt
3121 agaagggcgt attttcactg cattctccct atatgatgcg agaaatgtca ttaaaaatgg
3181 tgattttaat aatggcttat cctgctggaa cgtgaaaggg catgtagatg tagaagaaca
3241 aaacaaccaa cgttcggtcc ttgttgttcc ggaatgggaa gcagaagtgt cacaagaagt
3301 tcgtgtctgt ccgggtcgtg gctatatcct tcgtgtcaca gcgtacaagg agggatatgg
3361 agaaggttgc gtaaccattc atgagatcga gaacaataca gacgaactga agtttagcaa
3421 ctgcgtagaa gaggaaatct atccaaataa cacggtaacg tgtaatgatt atactgtaaa
3481 tcaagaagaa tacggaggtg cgtacacttc tcgtaatcga ggatataacg aagctccttc
3541 cgtaccagct gattatgcgt cagtctatga agaaaaatcg tatacagatg gacgaagaga
3601 gaatccttgt gaatttaaca gagggtatag ggattacacg ccactaccag ttggttatgt
3661 gacaaaagaa ttagaatact tcccagaaac cgataaggta tggattgaga ttggagaaac
3721 ggaaggaaca tttatcgtgg acagcgtgga attactcctt atggaggaat agtctcatgc
3781 aaactcaggt ttaaatatcg ttttcaaatc aattgtccaa gagcagcatt acaaatagat
3841 aagtaatttg ttgtaatgaa aaacggacat cacctccatt gaaacggagt gatgtccgtt
3901 ttactatgtt attttctagt aatacatatg tatagagcaa cttaatcaag cagagatatt
3961 ttcacctatc gatgaaaata tctctgcttt ttcttttttt atttggtata tgctttactt
4021 gtaatcgaaa ataaagcact aatagggtgt ttttgcccat cccttcggga aatcaagact
4081 aaaatgaaaa ataaacagaa aatataaggc tcttactttg tggatatgac cacaaagtaa
4141 gagccttatt tcattaaatt tgttcataca tttttccttg tagtcttttg ttttcatcct
4201 ttaatcgcct attctcgtac tctacttcct tgattcgatc ccgtaataat tgaatcattg
4261 catctttatt ttcatcactc attttccgtt tttcgaattt tggagataca gctcgttgct
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