GenBank-Updates@genbank.bio.net (02/21/91)
LOCUS ECOLASNII 1530 bp ds-DNA BCT 21-FEB-1991
DEFINITION E.coli L-asparaginase II (ansB) gene, complete cds.
ACCESSION M34277
KEYWORDS L-asparaginase II.
SOURCE E.coli (strain K-12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1530)
AUTHORS Jennings,M.P. and Beacham,I.R.
TITLE Analysis of the Escherichia coli gene encoding L-asparaginase II,
ansB, and its regulation by cyclic AMP receptor and FNR proteins
JOURNAL J. Bacteriol. 172, 1491-1498 (1990)
STANDARD simple staff_review
FEATURES Location/Qualifiers
mRNA 323..>1431
/note="ansB mRNA"
/evidence=EXPERIMENTAL
CDS 385..1431
/EC_number="3.5.1.1"
/note="L-asparaginase II (ansB) precursor"
/codon_start=385
sig_peptide 385..450
/note="L-asparaginase II signal peptide"
/codon_start=385
mat_peptide 451..1428
/EC_number="3.5.1.1"
/note="L-asparaginase II"
/codon_start=451
BASE COUNT 409 a 381 c 382 g 358 t
ORIGIN
1 aaatgggcgc gaaagcggtg ctgaaaagcg gcggtaaccc attacagaat gtgctgggaa
61 gcctgggaag cctggggggg ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
121 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
181 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
241 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
301 ttcacgttgt aaattgttta acgtcaaatt tcccatacag agctaaggga taatgcgtag
361 cgttcacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
421 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaaccggc
481 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagt gggtaaagtt
541 ggcgtagaaa atctggttaa tgcggtgccg caactaaaag acattgcgaa cgttaaaggc
601 gagcaggtag tgaatatcgg ctcccaggac atgaacgata atgtctggct gacactggcg
661 aaaaaaatta acaccgactg cgataagacc gacggcttcg tcattaccca cggtaccgac
721 acgatggaag aaactgctta cttcctcgac ctgacggtga aatgcgacaa accggtggtg
781 atggtcggcg caatgcgtcc gtccacgtct atgagcgcag acggtccatt caacctgtat
841 aacgcggtag tgaccgcagc tgataaagcc tccgccaacc gtggcgtgct ggtagtgatg
901 aatgacaccg tgcttgatgg ccgtgacgtc accaaaacca acaccaccga cgtagcgacc
961 ttcaagtctg ttaactacgg tcctctgggt tacattcaca acggtaagat tgactaccag
1021 cgtaccccgg cacgtaagca taccagcgac acgccattcg atgtctctaa gctgaatgaa
1081 ctgccgaaag tcggcattgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
1141 ctggtagatg cgggctatga tggcatcgtt agcgctggtg tgggtaacgg caacctgtat
1201 aaatctgtgt tcgacacgct ggcgaccgcc gcgaaaaccg gtactgcagt cgtgcgttct
1261 tcccgcgtac cgacgggcgc taccactcag gatgccgaag tggatgatgc gaaatacggc
1321 ttcgtcgcct ctggcacgct gaacccgcaa aaagcgcgcg ttctgctgca actggctctg
1381 acgcaaacca aagatccgca gcagatccag cagatcttca atcagtacta atcgcctcgc
1441 cccggtatcg tgccggggct ttttcacttc agactcacgt ccattgccaa ttttaattac
1501 cctaatgata atcaccggaa taaattattt
//