GenBank-Updates@genbank.bio.net (02/21/91)
LOCUS CIBRCI 4380 bp ds-DNA BCT 21-FEB-1991
DEFINITION Plasmid ColIb-P9 site-specific recombinase (rci) gene; complete
cds.
ACCESSION D90039 J03318
KEYWORDS inverted repeat; rci gene; recombinase; shufflon.
SOURCE Plasmid ColIb-P9 (from E.coli) DNA, clones pCL003-6, pCL004.
ORGANISM Plasmid Colicin Ib-P9
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 4380)
AUTHORS Kim,S.-R. and Komano,T.
TITLE Cloning and nucleotide sequence of the ColIb shufflon
JOURNAL Plasmid 22, 180-184 (1989)
STANDARD full staff_review
COMMENT These data kindly submitted in computer readable form by: Teruya
Komano Department of Biology Tokyo Metropolitan University
Fukazawa,Setagaya-ku Tokyo 158 Japan Phone: 03-717-0111 x3513 Fax:
03-725-8101
FEATURES Location/Qualifiers
misc_binding 228..230
/note="ribosome binding site (put.)"
repeat_region 1307..1325
/note="repeat 1"
repeat_region complement(1862..1880)
/note="repeat 2"
repeat_region 1889..1907
/note="repeat 3"
repeat_region complement(2363..2381)
/note="repeat 4"
repeat_region 2390..2408
/note="repeat 5"
repeat_region complement(2896..2914)
/note="repeat 6"
misc_signal 2917..2922
/note="-35 region (put.)"
misc_signal 2940..2945
/note="-10 region (put.)"
misc_binding 2957..2961
/note="ribosome binding site (put.)"
CDS 2966..4120
/note="site-specific recombinase (rci)(384 AA)"
/codon_start=2966
BASE COUNT 1191 a 1022 c 1035 g 1132 t
ORIGIN
1 aaccgccatc gtcttaatat tggcactgga gacctgtggc ttattgcagg tattaccgca
61 tggtcagggt tatataacgc catatggtgt gtgttgctgg gcacaggagg atttgtctta
121 tggcattcaa catggtgcat aaaagggcat aaagaaggcc ctctgggtcc atggctatgt
181 tttggccatg tccttttgct gctggataat ctttatcaac cactatgggt aatctgatga
241 aaaaatatga ccgaggctgg gcatcgctgg aaactggcgc ggctttactg attgttatgc
301 tcctgattgc ctggggagcg ggcatatggc aggactatat tcaaacaaaa ggatggcaaa
361 ctgaagcacg tcttgtcagc aactggacca gtgcggcccg ctcttatata gggaaaaact
421 acacgactct ccagggcagt agtaccacaa caactcctgc cgttatcacg acaaccatgc
481 tgaaaaatac cggctttttg tccagcgggt ttactgagac aaacagcgag gggcagcggt
541 tacaggcata tgtggttcga aacgcccaaa acccggaatt actacaggca atggttgtat
601 ccagtggtgg cacaccttat ccagtgaaag cacttatcca gatggctaag gatattacca
661 ctggtcttgg tggatatatc caggacggca aaacagccac aggtgcatta cgttcctggt
721 cagtagcttt aagtaattat ggtgccaaaa gcggtaacgg gcatattgcc gtattgttat
781 cgacagatga acttagtggt gcagctgagg acactgatcg tctttacaga ttccaggtca
841 atggtcgccc tgacttaaac aaaatgcaca cggccattga tatgggatca aataacctga
901 ataacgttgg ggcagtaaat gcccagacag gtaatttcag cggcaatgtg aatggtgtaa
961 atggcacttt cagcggtcag gtaaaaggca atagcggaaa ctttgacgta aatgtcaccg
1021 ctggcggtga tatcagaagt aataatggtt ggttaattac tcgtaacagt aaaggctggc
1081 tcaatgaaac tcacggtggc ggattttata tgtccgatgg atcatgggtt cgaagtgtaa
1141 acaacaaggg catctatacc ggcggtcagg tgaaaggcgg tactgttcgg gctgatggtc
1201 gcctttatac tggtgaatac ttacaactgg aaagaactgc cgttgctggc gcatcatgtt
1261 cgcctaacgg ccttgtaggc cgcgataata caggggcaat actttcgtgc caatccggta
1321 cgtggaagac ttctggttcg ctcaatggtt cttacacaaa cttaggttca catagaggtt
1381 cattctcagg gcggaattca gggggcagta cattgtttat ttatgcatct ggaggtaatg
1441 gaggatctgc cggaggtgca tgtgcaaata catcccgact gcagggatat gttggtggga
1501 cgctaattag cgtgaatgcc agtaataacc ctgcatatgg aaaaacagcc tttatcagct
1561 ttgctgtacc tgcaggtact tcctatcaga taacatccta tccaacagaa aatacatcat
1621 gtggtgccgg ggtattttca gtatttggat atcaaactta attgagcgtt acacacgacg
1681 caatacaacc tgtcttatgc tcgtatttgt accatttgcg tgaacctgct gggctctcaa
1741 ctacctggca gtagtgggca tcctctgcgt tgcccattct ggcaatggca cagaagtcga
1801 attgccccag ataacctgtg gtggaaagct gagtaacttt gagttttcca cctattgtcc
1861 cccacgtacc ggattggcac gtaggccagt gccaatccgg tacctggggc gctcctaaaa
1921 ttcaattcac aacgcagacc tacaacatag ctaaaaacac tcgtaattta aggctcgggg
1981 tccatgcata ttgctcatgg acttacctga atggctcacc ctttggtggt tttcaacagg
2041 tatattccga ccaaaacaac gtttggtatg tgagtaatta tgcttgggga aattatgagt
2101 ctggtgggac catatcagtc acatgcctca atcttcctgg tgctggagct taattaaagg
2161 ggcaacagta ggcatcgaca tgttcctcat caacaccatc accaataccg tacatgcgtg
2221 ttccagtcat aatgtatcca gtcggacatt gaatcggctt gtaatagatc gaaccaccac
2281 tcttaccgcc gataaagtga ttcattgcta cggatgattt gtaccaatta catgctgagt
2341 aattaatttt attaccccct gaccaccgac cggattggca ctggcctacg tgccaatccg
2401 gtacctggag aaaggtagga tctggtgagc ttcaaattgc tacagcacag gccacaggtt
2461 ggcgctttcc tggagcaaca gctacctgtc caactggaaa acgtgttaca ggtggcggcg
2521 gaatatgcac ctcacgaact ggttacatat ggcttaccag gtcattccca tcagccaata
2581 actcatggtc ggctgcgtgc gatacaaccg aagatcagaa tggttcaata actgtatatg
2641 ccatttgcca ataagctatt tcaagcaagt aatcttcata aagtaggacg aatactgtgt
2701 gtagtttttg gcttgccaag tcatctcgcc gttagagtca ggcatgtcaa taggaacgac
2761 ttctgtttct gccatttcac gcccgtcaat acgataggtg ttaatacaca gtttaaacct
2821 ccctaagacc tgtgtgtttt tgggatataa cgtaaatgtt tttatattcg tccatatcga
2881 cgctgatgat gatttccagg taccggattg gcacgcttgc agagaatgat tgttggcatt
2941 attttttgcc tcttttgagg ttagcatgcc gtctccacgc atccgtaaaa tgtccctgtc
3001 acgcgcactg gataagtacc tgaaaacagt ttctgttcac aagaaagggc atcaacagga
3061 gttttaccgg agcaatgtta tcaagcgata tcccattgcc cttcggaata tggacgaaat
3121 aacaaccgtt gatattgcta catacagaga cgttcgttta gcagaaatca acccccgaac
3181 gggtaaaccc attacaggta atactgtacg tcttgaactc gcccttctgt catctctgtt
3241 caatattgct cgtgttgaat ggggaacctg tcgtactaac ccggttgaac tggttcgcaa
3301 gccgaaagta tcctccggac gagatcgccg gctaacgtct tcagaagaac gtcgcctttc
3361 tcgctatttc cgcgaaaaaa atctgatgtt gtatgtcatt ttccatcttg cccttgagac
3421 agccatgcgg cagggcgaaa tactggcctt acgttgggag cacattgatt tgcgccacgg
3481 tgtggctcat ttacctgaaa ccaaaaacgg tcactcacgg gatgttcctc tgtccagacg
3541 tgcccgtaac tttcttcaga tgatgcccgt taatctccac ggcaatgttt ttgattacac
3601 cgcatccggc tttaaaaatg cctggagaat agccacacaa cgacttcgca tcgaggacct
3661 gcattttcac gatctacggc atgaagcaat aagccgcttc ttcgaactgg gtagcctgaa
3721 tgtaatggag attgctgcaa tatcaggaca tcgttccatg aatatgctga aacggtatac
3781 tcatcttcgt gcatggcaac tggtcagtaa acttgatgcc cgccggcggc agacacaaaa
3841 agtggcagca tggtttgtgc cgtatcctgc ccatatcacg accatcaatg aagaaaatgg
3901 gcagaaagcg catcgtattg agatcggtga ttttgataac cttcacgtca ctgccacaac
3961 gaaagaggaa gcagttcacc gcgccagtga ggttttgttg cgtacactgg ccattgcagc
4021 acagaaaggc gaacgtgtcc catctcccgg agcgttacct gttaacgacc ctgactacat
4081 tatgatttgc cctctgaacc cgggcagcac cccgctgtaa acatcattca aaacacatac
4141 agaaaggcga gttgcaacaa ctcgccaaac acattcgatc tgcatgatcg aaattattcg
4201 tttcaccaga aaaaacaccg aagatacaat aaccacatga gctgaaaaga ctttttcagt
4261 cggaggaaca atgagactga atacgaccgg tattgcggcc cgaatgatgc tctcgctgga
4321 caaaaaagca atcggcgaaa aactcatcaa cggtcgtcag ataaacccga cacctctgca
//