GenBank-Updates@genbank.bio.net (02/21/91)
LOCUS PPHIJ2 200 bp ds-DNA VRL 21-FEB-1991 DEFINITION Human papilloma virus type 16 genome, 3' virus-host integrated junction. ACCESSION D00487 KEYWORDS flanking sequences; integration; virus-host junction. SEGMENT 2 of 2 SOURCE Integrated human papillomavirus type 16 genome DNA in the cervical carcinoma cell line QG-U, clone UD913, EcoRI-HindIII fragment. ORGANISM Human papillomavirus Viridae; ds-DNA nonenveloped viruses; Papovaviridae; Papillomavirus. REFERENCE 1 (bases 1 to 200) AUTHORS Shirasawa,H., Tomita,Y., Fuse,A., Yamamoto,T., Tanzawa,H., Sekiya,S., Takamizawa,H. and Simizu,B. TITLE Structure and expression of an integrated human papillomavirus type 16 genome amplified in a cervical carcinoma cell line JOURNAL J. Gen. Virol. 70, 1913-1919 (1989) STANDARD full staff_entry COMMENT Sequence analyses revealed that the virus-host junctions reside at nucleotide positions 3741 and 4416. Part, 674 bp, of the viral genome (nucleotides 3742 to 4415) containing the putative early polyadenylation signal, was deleted. FEATURES Location/Qualifiers provirus <1..53 /note="HPV DNA (containing E2 ORF)" repeat_region 43..47 /note="inverted repeat" cellular 54..>200 /note="QG-U cellular DNA" repeat_region 73..77 /note="inverted repeat" BASE COUNT 55 a 49 c 44 g 52 t ORIGIN About 7.3 kb after segment 1; 8 bp upstream of PstI site. 1 atactgcagt gtcgtctaca tggcattgga caggacataa tgtaaaacat aaagggctgg 61 gaatatatat atttttaacc ttcaccccca tcccctcagg gaattcctaa tggtgggaat 121 caccagttca atccaccggc cagctttgcg gatgagaaca cagacagacc cagccactct 181 gttgagtttt gtgtctgctg //