GenBank-Updates@genbank.bio.net (02/21/91)
LOCUS PPHIJ2 200 bp ds-DNA VRL 21-FEB-1991
DEFINITION Human papilloma virus type 16 genome, 3' virus-host integrated
junction.
ACCESSION D00487
KEYWORDS flanking sequences; integration; virus-host junction.
SEGMENT 2 of 2
SOURCE Integrated human papillomavirus type 16 genome DNA in the cervical
carcinoma cell line QG-U, clone UD913, EcoRI-HindIII fragment.
ORGANISM Human papillomavirus
Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
Papillomavirus.
REFERENCE 1 (bases 1 to 200)
AUTHORS Shirasawa,H., Tomita,Y., Fuse,A., Yamamoto,T., Tanzawa,H.,
Sekiya,S., Takamizawa,H. and Simizu,B.
TITLE Structure and expression of an integrated human papillomavirus type
16 genome amplified in a cervical carcinoma cell line
JOURNAL J. Gen. Virol. 70, 1913-1919 (1989)
STANDARD full staff_entry
COMMENT Sequence analyses revealed that the virus-host junctions reside at
nucleotide positions 3741 and 4416. Part, 674 bp, of the viral
genome (nucleotides 3742 to 4415) containing the putative early
polyadenylation signal, was deleted.
FEATURES Location/Qualifiers
provirus <1..53
/note="HPV DNA (containing E2 ORF)"
repeat_region 43..47
/note="inverted repeat"
cellular 54..>200
/note="QG-U cellular DNA"
repeat_region 73..77
/note="inverted repeat"
BASE COUNT 55 a 49 c 44 g 52 t
ORIGIN About 7.3 kb after segment 1; 8 bp upstream of PstI site.
1 atactgcagt gtcgtctaca tggcattgga caggacataa tgtaaaacat aaagggctgg
61 gaatatatat atttttaacc ttcaccccca tcccctcagg gaattcctaa tggtgggaat
121 caccagttca atccaccggc cagctttgcg gatgagaaca cagacagacc cagccactct
181 gttgagtttt gtgtctgctg
//