GenBank-Updates@genbank.bio.net (02/21/91)
LOCUS ECOBGLTS 2971 bp ds-DNA BCT 21-FEB-1991
DEFINITION E.coli B glutamate carrier (gltS) gene, complete cds.
ACCESSION D00626
KEYWORDS gltS gene; glutamate carrier.
SOURCE E.coli B strain 29-78 DNA (genomic), clone pDES70 from cosmid
library.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2971)
AUTHORS Deguchi,Y., Yamato,I. and Anraku,Y.
TITLE Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene
of Escherichia coli B
JOURNAL J. Biol. Chem. 265, 21704-21708 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Ichiro Yamato
Department of Biological Science and Technology
Science University of Tokyo
2641 Yamazaki, Noda-shi
Chiba 278
Japan
Phone: 0471-24-1501 x4405
Fax: 0471-25-1841
FEATURES Location/Qualifiers
misc_signal 607..612
/note="-35 region"
misc_signal 630..635
/note="-10 region (Pribnow box)"
misc_binding 733..736
/note="SD sequence"
CDS 743..1948
/note="glutamate carrier"
misc_signal 1979..1999
/note="rho-independent transcription terminator"
BASE COUNT 624 a 692 c 830 g 825 t
ORIGIN 1 bp upstream of EcoRI site.
1 gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
61 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
121 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
181 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
241 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
301 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
361 gaccacaccc gtcctgtgga tcttcaagac ggtaaatcag ttcgctgttt tgagtctgcg
421 caaccggttg cgcattttct gactcgaggg tggaaacaga catcgaaaac aatcccgtgg
481 tagcaaagcg ggcattttag ctgaccgaaa gagaaaagca aacgtttgcc acatcctcat
541 ctaaatattt aaatgagata aatgcacttt tttatataac ttttggtatt tttctgctta
601 aaatccatgc catttgttca ttaacagctt agaatttgac ccaggaaagc ataactcgat
661 acccgtcata gttcatgaag ttgccactgc gcgatgaagt atgacgagta tgaaagagtg
721 atgcggatac aaaggagtaa ctatgtttca tctcgatact ttagcaacgc ttgttgccgc
781 aacgctgacg ttgctgctcg ggcgtaagtt ggtccattcc gtctcctttt tgaagaaata
841 caccataccg gaacctgttg cgggtggttt gttggtggcg ctggcgctac tagtactgaa
901 aaaaagcatg ggctgggaag tcaactttga tatgtccctg cgcgatccgt taatgctggc
961 tttcttcgcc accattggcc tgaacgccaa cattgccagt ttgcgtgccg gtgggcgtgt
1021 ggttggcatc ttcttgattg tggttgttgg tctgttggtg atgcaaaatg ccattggcat
1081 tggtatggct agcttgttag ggcttgatcc gctgatgggg ctgttggccg gttctattac
1141 gctttccggc ggtcacggta cgggcgctgc gtggagtaaa ttgttcattg aacgttatgg
1201 cttcaccaat gcgacggaag tggcgatggc ctgtgcaacg ttcggtctgg tgctgggcgg
1261 cttgattggc ggtccggtgg cgcgctatct ggtgaaacac tccaccacgc cgaacggtat
1321 tccggatgac caggaagtcc cgacggcgtt tgaaaagccg gatgtgggac gcatgatcac
1381 ctcgttggtg ctgattgaaa ctatcgcgct gattgctatc tgcctgacgg tggggaaaat
1441 tgttgcgcaa cttttggctg gcactgcttt tgaactgccg accttcgtct gtgtactgtt
1501 tgttggcgtg attctgagca acggtctgtc aataatgggc ttttaccgcg tctttgagcg
1561 tgcggtatcc gtgctgggta acgtaagctt gtcgttgttc ctggcgatgg cgctgatggg
1621 gctgaaactg tgggagctgg cttcgctggc gctgccgatg ctggcgattc tggtggtaca
1681 gaccatcttc atggcgttgt atgccatctt cgttacctgg cgcatgatgg gcaaaaacta
1741 cgatgcggca gtgctggctg cgggtcactg tggttttggc ctcggtgcaa cgccaacggc
1801 aatcgccaac atgcaggcga tcactgaacg ctttggcccg tcgcacatgg cgtttttggt
1861 ggtgccgatg gtcagtgcgt tctttatcga tatcgtcaat gcgctggtga ttaagctgta
1921 tttaatgttg ccgatttttg ccggttaaaa atgaaaaagc gtcggaagga accggaaacg
1981 ctacgtgaac actgtcgcca tatttttggt gatgagccgc cagtgctctg tgtatgggaa
2041 acggaatttg attacgcaga tgccgaactc aaggcgctgg cggctaaaga gtggcagcag
2101 atcagcgaat gggatttgag tgcttactat gtactcaatc tcgtttataa cgaaccgatg
2161 caaatagagt tgtttcgcta tctgtttccg ctctgcctgg cgcaatggca tgaaaccgtt
2221 ttagctggcg gttatggtga tcacttcgaa gatccttaat gaaggcattg tgctcggccc
2281 tatctctggc aggagatgat gaatgcttcc caacgccagc aggtacggca atttttactg
2341 gacaccgcgt tgcagcgtat ggataacgag cgtggtttca ataacgttct ttgctggctg
2401 gcggtgttca atactctggg tggggccgcg ccgttaattc gttctctctg gtcgcgctgg
2461 tgggcgctgg atacgccagg aaaagcggtg tgtgctattc agtatgccgc gcatctaatt
2521 tatcccatag aagccaatcc gttatggtcg caagaatgga taggctgggg gcatccatta
2581 ggccataaag atggctggtc ctctgataat cgcgcttttc ttcgccaaat gttaacacct
2641 gaaatgattg tggctggcgt acaggcagcg gctgaaattc tgcgtggtga accggaaggc
2701 gcaatggcgg cgcgaattgc acaggatgcg tatgaggcaa tggatatcct cactatccag
2761 attgaagatt tactgcgtga cttgtcttgt gatgagtctg gtcatgcgtt ggagtaacgt
2821 tccgtctccg gcatccagcg ttctatcagg gcttttgctt gttgtgggta acgttcgtga
2881 atatgtcgcg ccaggcgctg aacttccggg atcatcgcct gatcgcgcag taaatccgcc
2941 actttaaact cagcattacc cgtctgacgc g
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