GenBank-Updates@genbank.bio.net (02/12/91)
LOCUS BBRHWPA 4330 bp ds-DNA BCT 12-FEB-1991
DEFINITION B.brevis HWP gene, complete cds.
ACCESSION D90050 M33802
KEYWORDS HWP protein; cell wall protein; surface layer protein.
SOURCE B.brevis (strain HPD31) DNA.
ORGANISM Bacillus brevis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4330)
AUTHORS Ebisu,S., Tsuboi,A., Takagi,H., Naruse,Y., Yamagata,H.,
Tsukagoshi,N. and Udaka,S.
TITLE Conserved structures of cell wall protein genes among protein-
producing Bacillus brevis strains
JOURNAL J. Bacteriol. 172, 1312-1320 (1990)
STANDARD full staff_review
COMMENT These data kindly submitted in computer readable form by:
Norihiro Tsukagoshi
Department of Food Science and Technology
Faculty of Agriculture,
Nagaya University Furo-cho, Chikusa-ku Nagoya 464 Japan
Phone: 052-781-5111 x6869 Fax: 052-781-4447
FEATURES Location/Qualifiers
misc_feature 132..132
/note="transcription initiation site"
misc_feature 255..255
/note="transcription initiation site"
misc_feature 325..325
/note="transcription initiation site"
misc_feature 373..373
/note="transcription initiation site"
misc_feature 386..386
/note="transcription initiation site"
CDS 548..3898
/note="HWP protein precursor (ttg start codon)"
/codon_start=1
sig_peptide 548..706
/note="HWP protein signal peptide"
/codon_start=1
mat_peptide 707..3895
/note="HWP protein"
/codon_start=1
BASE COUNT 1401 a 804 c 999 g 1126 t
ORIGIN
1 atcgattacc aaaaaaccgc gaaagattcc gcggctgaga gctatcgttt gaccaatttg
61 cgctttgaaa cggtctggca acgaccctcg aagtgattca gccgaggagg aattatccaa
121 ccgtgagaat cagtaccaaa aagcgatcct gcagtacaat ctggctgtgg taaactttga
181 aactgccctc ggaagctaag aagaatttgt aaggttttga atggaactgg aaaaaggttc
241 aatggtgaca gtccgtccgg cgtaccctat aatacgagtt gtggcggatg tcactgctta
301 catattacaa gtgaatacga cccatgaaaa tttctttaaa ctttttttct gaggcgccgc
361 aacttttgct cgactgaggc gtttaatagg gtgtcacacg aaaaacgggg aattgtgtaa
421 aaaagattca cggattctag catttgtgtt acactagtga ttgttacatt ttacacaata
481 gctgaatata ctagagattt ttaacacaaa aaagcgaagc tgtcctgcga aaggaggtga
541 cacgcgcttg caggattcgg gctttaaaaa gaaagataga tcaacaaata ttccccaaga
601 acaatttgtt tatactagag gaggagaaca caaggttatg aaaaaggtcg ttaacagtgt
661 attggctagt gcgctcgcca tcacagttgc tccaatggct ttcgcagcag aggacacaac
721 tacagctcct aaaatggatg ctgcaatgga gaaaaccgta aaacgtctgg aagctcttgg
781 cctggtagca ggttatggca acggcgattt cggtgcagac aaaaccatca ctcgtgcaga
841 gttcgctact ctaatcgttc gcgctcgcgg tctggagcaa ggtgcgaaac tggcacaatt
901 caacactact tacacagatg ttagatccac tgactggttc gctggttttg taaacgtagc
961 ttccggtgaa gaaatcgtaa aaggtttccc ggacaaatct tttaaaccac aaaaccaagt
1021 tacttatgct gaagcagtaa ccatgatcgt tcgtgctttg ggttatgagc catccgttcg
1081 aggtgtatgg ccgaacagca tgatctccaa aggttccgaa ctgaacattg caaaaggtat
1141 caacaaccct aacatgcagc agttcgcggc gacaatcttc aaaatgctgg acaacgctct
1201 tcgcgttaag ctgatggagc aaatcgaata cggtactgac atccgtttaa acgtaactga
1261 cgaaactctc ttgactaaat atttgaaagt taccgtacgt gatatggact gggctcacga
1321 aaagggtaac aattctgatg aattgccact tgtaacaaac gtacctgcta ttggtctggg
1381 tagtttgaaa gcaaatgaag ttactttgaa tggaaaagat gctgatctgg gtagcaacac
1441 tacttataaa gtagctgaag gcatcaatcc taacgcattt gatggtcaaa aagtacaagt
1501 gtggatcaaa gatgaccgag aaaatgtcat cgtttggatg gaaggttccg aagacgaaga
1561 tgtcgttatg gaccgtgtga gtgctctgta cctgaaaggt aaagccttca cagatgatat
1621 tgtaaaagat cttagcaagt ctgatttgga tgatgtaaaa atcgaaatgg atggtagcga
1681 gaaatcgtat cgcttgacgg aagatacgaa aatcacttat aacttcactc gtttcaacga
1741 tccagtagat gctctgagca aaatttacaa agacaacgac acgtttggtg ttaaagttgt
1801 tttgaatgat aacaatgaag ttgcatacct ccacatcatt gacgatcaaa caattgataa
1861 aagcgtaaaa ggcgttaaat acggttccaa agttattagc aaaatcgatg ctgataagaa
1921 gaaaatcacc aacttggata actccaaatt tagcgacttg gaagatcagg atgaaggaaa
1981 agacttcttg gtattccttg atggccaacc agctaaactg ggtgatctga aagaatccga
2041 tgtttactcg gtatactatg ctgatggtga taaagataag tacttggtgt ttgctaaccg
2101 taacgttgca gaaggaaaag tagaaaaagt ggtaagccgt aacaagacag acattcgtct
2161 gactgttggt ggtaaaactt acaaagtata tccagatgct tcttactccg aaaacgcaaa
2221 taaagatgtt aagaaggtta attccgactt ggatctgatt agcaaccttg atggagaaga
2281 agtaaaactt ctcttggatc catccggtcg agttcgtcac atcgaaacaa aagacgcgat
2341 cgatgatcgt aaaccattgg caatcatcac taagggtgct acctataact ccagcaaaga
2401 tacttatgac tttactgtta tgacccaaaa aggtaaaaca caaatcgtat ctttggatca
2461 aaaggacatc tacgatagat atggggttaa ctacgataaa tccaatgata agcgtcaagc
2521 tttcgaaaag gatttagttg agcttctgca acctaaggta gttaaagagg actcagctac
2581 tgatgccaat caaaccgttc ttttggaagt taattttgat tccaaaggcg aagttgataa
2641 agtcaaagtt cttgatagca agctgaaata ctccgagaaa agcacttggg ataaacttgc
2701 ggatgaagat gacgacgtcg ttggtgatta tgaagtaacc gataagactg ctgtcttcaa
2761 aatgactggt gaccttactc cagctactgg aactaaacgt ggtgaactga aaaacgctgg
2821 tactgctaaa tttaaggatg ttgctaagaa gagcgatctg aaggtttggt actcagtgga
2881 tgaagacaaa ggtgaagttc aagcgatctt cgtagtagat ggcagtggtt tgggtggcga
2941 ccatcagttt ggtatggtca aacaatatgg tactgctagt aaacaggaca ctatcactat
3001 agttacaaaa gatggtgatt ctgtcactga aaaagagtac aaattggatg gcgacgctga
3061 tgatttgaaa gttgatcaag acatcagacg tggagatgtc atttctttca cactgaattc
3121 tgatggagaa gttattgttg acgatgtagt cgaggttgta aataacaacc acattgataa
3181 cactgcttct aaatcagcta cgctcatgcc tgaagacgaa cgtcaaaaag caggaatcga
3241 caaattggtt gttgctcgcg ttgacgaagt tgatggtaac actatttcct tgaactatgc
3301 tgacggaaag acacaaaaat attacacaaa agcatccact gcgttcattg atgtgtatga
3361 cggtcttgaa ggaattgatg gagtagatga aggcgactac atcgtaatga tcgatagcgc
3421 cgatattgac ggaactcgct ttgactatgt actggtagtt tctagcgatg atgagatccg
3481 cacgcaacac atctccacta aagcagttac ggacttcctg aacaagccaa ccagactatg
3541 taccaaatcc tggcgatggg gaagaagtag tcacggcacc aaagttaata cagttaacga
3601 tgaagcagtt gtagatggta ttgtaactct tccagctgat gcatctgtta gaaacttcaa
3661 cattgcattt gatcaagaaa ttaacagcaa agatgcaacg gtaactgtta ctaatgaaga
3721 tacgcttggt aacgtaacgg tatctgaggt tgcgacagat gcaaaagtat tgagcttcaa
3781 gactgctaaa ctggacacta caaaaactta catcatcaca gttaaaggct tgaaagataa
3841 aaacggtaaa gcagtgaaag atgtaactct ttacgttgaa tttgttgctg gagtgtgact
3901 tgttgctcta taaacactaa tagtgtgaaa tgagtgaaga agggagaagg attaaaacct
3961 tctcccttct ttaactgttt tggcgaattg aagggtgtaa cccccttccg tggaaacaac
4021 cattaatcca cgtgactaat cctcctgcat gcgttgtaag ctaactctta cagggtgtga
4081 agccaggtga agacgtaacc cttagtggtg cgggtctccg taaaagctaa ctatggttag
4141 gaaacgaatc tacgtctgag gcgtgtcagg tttggtaatt agcccttcag ctgctaatta
4201 ccccacaggc tgagatgcct ggagggttct aacgaattaa ggttgttaac gttagttcat
4261 cttgatatgt atagtaggaa cctaaggttt agcaaaaaaa gggatagcca aaatggaaca
4321 agtgagatct
//GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS BBRHWPA 4330 bp ds-DNA BCT 22-FEB-1991
DEFINITION B.brevis HWP gene, complete cds.
ACCESSION D90050 M33802
KEYWORDS HWP protein; cell wall protein; surface layer protein.
SOURCE B.brevis (strain HPD31) DNA.
ORGANISM Bacillus brevis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4330)
AUTHORS Ebisu,S., Tsuboi,A., Takagi,H., Naruse,Y., Yamagata,H.,
Tsukagoshi,N. and Udaka,S.
TITLE Conserved structures of cell wall protein genes among protein-
producing Bacillus brevis strains
JOURNAL J. Bacteriol. 172, 1312-1320 (1990)
STANDARD full staff_review
COMMENT These data kindly submitted in computer readable form by:
Norihiro Tsukagoshi
Department of Food Science and Technology
Faculty of Agriculture,
Nagaya University Furo-cho, Chikusa-ku Nagoya 464 Japan
Phone: 052-781-5111 x6869 Fax: 052-781-4447
FEATURES Location/Qualifiers
misc_feature 132..132
/note="transcription initiation site"
misc_feature 255..255
/note="transcription initiation site"
misc_feature 325..325
/note="transcription initiation site"
misc_feature 373..373
/note="transcription initiation site"
misc_feature 386..386
/note="transcription initiation site"
CDS 548..3898
/note="HWP protein precursor (ttg start codon)"
/codon_start=548
sig_peptide 548..706
/note="HWP protein signal peptide"
/codon_start=548
mat_peptide 707..3895
/note="HWP protein"
/codon_start=707
BASE COUNT 1401 a 804 c 999 g 1126 t
ORIGIN
1 atcgattacc aaaaaaccgc gaaagattcc gcggctgaga gctatcgttt gaccaatttg
61 cgctttgaaa cggtctggca acgaccctcg aagtgattca gccgaggagg aattatccaa
121 ccgtgagaat cagtaccaaa aagcgatcct gcagtacaat ctggctgtgg taaactttga
181 aactgccctc ggaagctaag aagaatttgt aaggttttga atggaactgg aaaaaggttc
241 aatggtgaca gtccgtccgg cgtaccctat aatacgagtt gtggcggatg tcactgctta
301 catattacaa gtgaatacga cccatgaaaa tttctttaaa ctttttttct gaggcgccgc
361 aacttttgct cgactgaggc gtttaatagg gtgtcacacg aaaaacgggg aattgtgtaa
421 aaaagattca cggattctag catttgtgtt acactagtga ttgttacatt ttacacaata
481 gctgaatata ctagagattt ttaacacaaa aaagcgaagc tgtcctgcga aaggaggtga
541 cacgcgcttg caggattcgg gctttaaaaa gaaagataga tcaacaaata ttccccaaga
601 acaatttgtt tatactagag gaggagaaca caaggttatg aaaaaggtcg ttaacagtgt
661 attggctagt gcgctcgcca tcacagttgc tccaatggct ttcgcagcag aggacacaac
721 tacagctcct aaaatggatg ctgcaatgga gaaaaccgta aaacgtctgg aagctcttgg
781 cctggtagca ggttatggca acggcgattt cggtgcagac aaaaccatca ctcgtgcaga
841 gttcgctact ctaatcgttc gcgctcgcgg tctggagcaa ggtgcgaaac tggcacaatt
901 caacactact tacacagatg ttagatccac tgactggttc gctggttttg taaacgtagc
961 ttccggtgaa gaaatcgtaa aaggtttccc ggacaaatct tttaaaccac aaaaccaagt
1021 tacttatgct gaagcagtaa ccatgatcgt tcgtgctttg ggttatgagc catccgttcg
1081 aggtgtatgg ccgaacagca tgatctccaa aggttccgaa ctgaacattg caaaaggtat
1141 caacaaccct aacatgcagc agttcgcggc gacaatcttc aaaatgctgg acaacgctct
1201 tcgcgttaag ctgatggagc aaatcgaata cggtactgac atccgtttaa acgtaactga
1261 cgaaactctc ttgactaaat atttgaaagt taccgtacgt gatatggact gggctcacga
1321 aaagggtaac aattctgatg aattgccact tgtaacaaac gtacctgcta ttggtctggg
1381 tagtttgaaa gcaaatgaag ttactttgaa tggaaaagat gctgatctgg gtagcaacac
1441 tacttataaa gtagctgaag gcatcaatcc taacgcattt gatggtcaaa aagtacaagt
1501 gtggatcaaa gatgaccgag aaaatgtcat cgtttggatg gaaggttccg aagacgaaga
1561 tgtcgttatg gaccgtgtga gtgctctgta cctgaaaggt aaagccttca cagatgatat
1621 tgtaaaagat cttagcaagt ctgatttgga tgatgtaaaa atcgaaatgg atggtagcga
1681 gaaatcgtat cgcttgacgg aagatacgaa aatcacttat aacttcactc gtttcaacga
1741 tccagtagat gctctgagca aaatttacaa agacaacgac acgtttggtg ttaaagttgt
1801 tttgaatgat aacaatgaag ttgcatacct ccacatcatt gacgatcaaa caattgataa
1861 aagcgtaaaa ggcgttaaat acggttccaa agttattagc aaaatcgatg ctgataagaa
1921 gaaaatcacc aacttggata actccaaatt tagcgacttg gaagatcagg atgaaggaaa
1981 agacttcttg gtattccttg atggccaacc agctaaactg ggtgatctga aagaatccga
2041 tgtttactcg gtatactatg ctgatggtga taaagataag tacttggtgt ttgctaaccg
2101 taacgttgca gaaggaaaag tagaaaaagt ggtaagccgt aacaagacag acattcgtct
2161 gactgttggt ggtaaaactt acaaagtata tccagatgct tcttactccg aaaacgcaaa
2221 taaagatgtt aagaaggtta attccgactt ggatctgatt agcaaccttg atggagaaga
2281 agtaaaactt ctcttggatc catccggtcg agttcgtcac atcgaaacaa aagacgcgat
2341 cgatgatcgt aaaccattgg caatcatcac taagggtgct acctataact ccagcaaaga
2401 tacttatgac tttactgtta tgacccaaaa aggtaaaaca caaatcgtat ctttggatca
2461 aaaggacatc tacgatagat atggggttaa ctacgataaa tccaatgata agcgtcaagc
2521 tttcgaaaag gatttagttg agcttctgca acctaaggta gttaaagagg actcagctac
2581 tgatgccaat caaaccgttc ttttggaagt taattttgat tccaaaggcg aagttgataa
2641 agtcaaagtt cttgatagca agctgaaata ctccgagaaa agcacttggg ataaacttgc
2701 ggatgaagat gacgacgtcg ttggtgatta tgaagtaacc gataagactg ctgtcttcaa
2761 aatgactggt gaccttactc cagctactgg aactaaacgt ggtgaactga aaaacgctgg
2821 tactgctaaa tttaaggatg ttgctaagaa gagcgatctg aaggtttggt actcagtgga
2881 tgaagacaaa ggtgaagttc aagcgatctt cgtagtagat ggcagtggtt tgggtggcga
2941 ccatcagttt ggtatggtca aacaatatgg tactgctagt aaacaggaca ctatcactat
3001 agttacaaaa gatggtgatt ctgtcactga aaaagagtac aaattggatg gcgacgctga
3061 tgatttgaaa gttgatcaag acatcagacg tggagatgtc atttctttca cactgaattc
3121 tgatggagaa gttattgttg acgatgtagt cgaggttgta aataacaacc acattgataa
3181 cactgcttct aaatcagcta cgctcatgcc tgaagacgaa cgtcaaaaag caggaatcga
3241 caaattggtt gttgctcgcg ttgacgaagt tgatggtaac actatttcct tgaactatgc
3301 tgacggaaag acacaaaaat attacacaaa agcatccact gcgttcattg atgtgtatga
3361 cggtcttgaa ggaattgatg gagtagatga aggcgactac atcgtaatga tcgatagcgc
3421 cgatattgac ggaactcgct ttgactatgt actggtagtt tctagcgatg atgagatccg
3481 cacgcaacac atctccacta aagcagttac ggacttcctg aacaagccaa ccagactatg
3541 taccaaatcc tggcgatggg gaagaagtag tcacggcacc aaagttaata cagttaacga
3601 tgaagcagtt gtagatggta ttgtaactct tccagctgat gcatctgtta gaaacttcaa
3661 cattgcattt gatcaagaaa ttaacagcaa agatgcaacg gtaactgtta ctaatgaaga
3721 tacgcttggt aacgtaacgg tatctgaggt tgcgacagat gcaaaagtat tgagcttcaa
3781 gactgctaaa ctggacacta caaaaactta catcatcaca gttaaaggct tgaaagataa
3841 aaacggtaaa gcagtgaaag atgtaactct ttacgttgaa tttgttgctg gagtgtgact
3901 tgttgctcta taaacactaa tagtgtgaaa tgagtgaaga agggagaagg attaaaacct
3961 tctcccttct ttaactgttt tggcgaattg aagggtgtaa cccccttccg tggaaacaac
4021 cattaatcca cgtgactaat cctcctgcat gcgttgtaag ctaactctta cagggtgtga
4081 agccaggtga agacgtaacc cttagtggtg cgggtctccg taaaagctaa ctatggttag
4141 gaaacgaatc tacgtctgag gcgtgtcagg tttggtaatt agcccttcag ctgctaatta
4201 ccccacaggc tgagatgcct ggagggttct aacgaattaa ggttgttaac gttagttcat
4261 cttgatatgt atagtaggaa cctaaggttt agcaaaaaaa gggatagcca aaatggaaca
4321 agtgagatct
//